甘蔗抗虫机制的研究进展

甘蔗是重要的糖料作物也是潜在的能源作物,而甘蔗的生长过程中长期受到各种虫害威胁。目前用于防治甘蔗害虫主要是通过化学防治和生物防治,带来的害虫耐药性和环境安全问题不容忽视。选育和利用抗虫品种,挖掘甘蔗自身抗虫特性是目前公认的最根本、经济有效和对环境影响最少的办法。笔者首先介绍了不同甘蔗品种对甘蔗螟虫的抗性等级,再从甘蔗的物理抗性和生理生化抗性2个方面阐述甘蔗的抗虫原理,并重点介绍了甘蔗的诱导型生理生化防御体系。全文还就目前甘蔗抗虫研究现状提出建议和展望,以期为培育甘蔗抗性品种以及蔗田害虫综合治理提供参考。     

Detection and Pharmacokinetics of Etoricoxib in Thoroughbred Horses

Etoricoxib, a selective inhibitor of cyclooxygenase-2, is used in the treatment of many inflammatory diseases and dental pain in humans. The aim of this study was to determine the pharmacokinetics and metabolism of etoricoxib in horses. Six horses weighing an average of 475 ± 25 kg were administered a single oral dose of etoricoxib at 1 mg/kg body weight. The results show that the drug reached a maximum concentration of 505.2 ± 67.8 ng/mL in 48 minutes after administration. The elimination half-life was calculated to be 10.20 ± 1.30 hours. Mass spectrometric analysis confirmed that etoricoxib is metabolized in horses via the oxidation of its 6′-methyl group to form a hydroxyl methyl etoricoxib which can further be oxidized to form either an acid or be glucuronidated. In addition, the 1′-N terminal of 6′-hydroxymethyl metabolite is oxidized to form the corresponding 1′-N oxide metabolite. The present results have clearly demonstrated that etoricoxib is mainly excreted in urine as metabolites. From these data, it is also possible to postulate a detection time for the metabolites which in turn can assist in the control of illegal use of the drug in horse racing.     

小麦灌浆后期青枯骤死原因分析及控制

小麦灌浆后期植株衰老与活性氧自由基毒害和膜脂质过氧化密切相关。灌浆后期株体内活必砼谢平衡失调是青枯发生的主要内因：造成青枯的外因除与“Ｖ”型变温有关外，雨后骤晴的强光与高温协同作用亦是青枯发生的重要外因一。灌浆后期“Ｖ”型变温使麦株各器均受到伤害，尤其穗下茎受到伤害。青枯能否发生取决于植体内酶活力的高低、非酶清除剂含量的多少以及“Ｖ”型变温的强度和持续时间。选择优良品种、适时播种、合理施肥和加强     

酵母蛋白饲喂泌乳奶牛试验报告

本试验通过在荷斯坦泌乳牛日粮中添加酵母蛋白部分替代豆粕,研究其对泌乳牛干物质采食量、产奶量、乳成分及身体健康情况的影响,选取400头胎次、产奶量相近的中国荷斯坦泌乳牛分为对照组和试验组各200头,试验组每头牛每天饲喂500g酵母蛋白,通过60d的试验,结果表明试验组和对照组奶牛平均日产奶量差异不显著（P〉0.05）,分别为32.54kg/d与32.34kg/d。在产奶量不变的基础上,试验组平均干物质采食量降低1.7kg（DM）/d,产奶效率提高,与对照组相比差异极显著（P〈0.01）。     

阴阳离子平衡对山羊瘤胃内环境及养分消化代谢的影响

选用安有永久瘤胃瘘管黄淮山羊6只,随机分为3组,分别饲喂3种不同阴阳离子平衡（A组332mEq／kgDM、B组418mEq／kgDM、C组496mEq／kgDM）的日粮,研究日粮阴阳离子平衡对瘤胃消化代谢的影响。结果表明：提高日粮阴阳离子水平可以有效提高瘤胃液的pH值,在饲喂后8h差异显著（P〈0．05）;青干草DM、CF的降解率在瘤胃内随时间的增加而增加,在同一时间段试验B组较高,试验A组最低,且在12h、24h、48h时组间差异显著（P〈0．05）。CF的降解率趋势与DM相同,组间差异显著（P〈0．05）。     

芒果蒂腐病菌对芒果采后乙烯释放和ACC含量的影响

测定芒果蒂腐病菌(Botryodiplodia theobromae)对芒果果实乙烯代谢的影响,结果表明:B.theobromae在芒果果肉培养基上不产生乙烯,但是B.theobromae代谢活动能诱导芒果果肉中产生大量的1-氨基环丙烷-1-羧酸(ACC),导致芒果快速释放乙烯,随着发病程度增加和贮藏期延长,乙烯释放速率快速增加。高浓度乙烯使芒果果肉快速软化,在25℃条件下,果实在发病后3～5 d就完全腐烂。     

The effect of temperature on the energy budget of the Manila clam, <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>

In this study, the energy budget of the Manila clam, Ruditapes philippinarum, was evaluated after one-week acclimation periods at 5, 10, 15, 20, and 25°C. Small clams (151 ± 12 mg DW) and large clams (353 ± 16 mg DW) were fed with the microalgae, Isochrysis galbana. Filtration rate, ingestion rate, assimilation efficiency, oxygen-consumption rate, and ammonia excretion rate were measured. Both filtration rate and ingestion rate of small and large clams were found to be related to temperature. The highest Q ₁₀ values were measured in the range 15–20°C for both small and large clams. Assimilation efficiency of both small and large clams was not significantly influenced by temperature, although the maximum mean values were detected at 20°C. Oxygen consumption rate and ammonia excretion rate of small and large clams were found to be related directly to temperature over the entire range, with a maximum being detected at 25°C. The highest Q ₁₀ value was estimated in the range 10–15°C with regard to oxygen consumption rate, and in the range of 15–20°C with regard to ammonia excretion rate. Scope for growth (SFG) was positive at all temperatures, achieving a maximum value at 20°C in both small and large clams, primarily as a consequence of the enhanced ingestion rate which offset the concomitant elevation in the metabolic rate. In this study we have estimated the thermal optimum for this species at 20°C.     

3H—萱草根素在小鼠体内吸收分布,排泄和代谢的初步观察

本文应用放射性同位素示综技术,初步观察了~3H-萱草根素在小鼠体内吸收分布、排泄和代谢的全过程。~3H-萱草根素口服后0.5小时,小鼠血液中放射性即达到高峰,并以低浓度维持96小时以上;其随血液循环分布于各组织器官,以胃肠组织含量最高,其次是肝脏和脾脏。萱草根素有蓄积性;一次给药和以同等剂量分次(每天一次,三天)投药后的相同时间,各组织中~3H-萱草根素的含量差异不显著。主要排泄途径是粪便,其次是尿液。经有机溶剂处理后的尿液分为水、脂二相,其放射性测量和薄板层析放射自显影结果均表明:~3H-萱草根素在体内经过代谢后结构发生了变化,从尿液中排出的不是萱草根素原形,而是其代谢产物。     

放牧气候环境因素对泌乳母羊消化代谢的影响

试验选取6只内蒙古细毛羊健康泌乳母羊，分别于泌乳前期（Ⅰ、Ⅱ期）、泌乳中期（Ⅲ期）和泌乳后期（Ⅳ期），采用消化代谢、气体能量代谢等试验方法，运用灰色关联度分析法，探讨放牧条件下主要气候因素（气温、气湿、风速）对母羊不同泌乳期营养物质消化代谢的影响。结果表明，在本试验条件下，试验羊产热量受风速影响最大，机体能量存留量受气温影响最明显，而来食量、消化率、N存留量及总能代谢率等项指标与空气湿度关系最密切。