Preparation and Identification of Monoclonal Antibody Against Swainsonine

In this test,BALB/c mice were immunized by SW-OVA for the preparation of monoclonal antibody against swainsonine (SW). Hybridoma cells that could secrete specific antibody against SW were prepared by hybridoma technique,and then the strain that secreted monoclonal antibody against SW designated 1F10 was prepared,and the chromosome average number of 1F10 cell was 45 to 50 couples. The ELISA titers of cell supernatant were 1:25 600, and that of ascites were 1:80 000.The subclasses of monoclonal antibody was IgG1,and the affinity constant was 1.14×10¹⁰,the purity of ascites antibodies was up to 98%,and the recovery rate was 80%.The result of sodium dodecyl sulfate polyacrylamide gel electropheresis proved that purified antibody had been obtained that the molecular weight of H-chain and L-chain of antibody was about 50 and 25 ku. The monoclonal antibody against SW specifically bound to SW determined by Western blotting. The linear range was 4 to 128 μg/mL (R²=0.9969) and no cross-reactivity was detected with BSA,gelatin,polylysine,me-Gal,etc. The result laid the foundation for immunodetection on SW and immunological prevention of animal toxic disease.     

SPF鸡感染网状内皮组织增生症病毒后血清和卵黄的抗体反应相关性比较

为进一步研究采用卵黄抗体检测代替血清抗体检测的可行性，采用已建立的卵黄抗体ELISA检测方法，比较同一时期卵黄抗体与血清抗体的相关性。人工感染23周龄无特定病原体（SPF）鸡，每周采集全血分离血清，每天收集种蛋，ELISA方法检测血清、种蛋中的REV抗体，并进行比较，结果表明：攻毒后第2周开始血清抗体和卵黄抗体呈阳性，并达到峰值，之后抗体水平缓慢下降，两者具有相似的消长规律；对同一时期的卵黄抗体和血清抗体S/P比值进行统计学比较，P值小于0.05，相关系数为0.931，表明两者差异性不显著，相关性很好；阴阳性符合率比较，阳性符合率为97.8%，阴性符合率为100%，总体符合率为98.4%，阴阳性复合率很高。试验证明，同一时期的卵黄抗体和血清抗体的相关性很好，可用卵黄抗体检测方法代替血清抗体检测，从而为监测SPF鸡群感染REV提供新的技术手段。