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鱼源小肠结肠炎耶尔森氏菌的耐药性研究   总被引:1,自引:1,他引:0       下载免费PDF全文
本试验采用聚合酶链反应(PCR)方法检测鱼源小肠结肠炎耶尔森氏菌的四环素类耐药基因(tetA、tetC、tetM)、磺胺类耐药基因(sul1、sul2、sul3)和氨基糖苷类耐药基因(aph(3')-Ⅲa、aac(6')-Ⅰb、ant(3')-Ⅰa、aac(3)-Ⅱa),并采用κ-B法检测该菌对14种抗生素的耐药表型。结果表明,小肠结肠炎耶尔森氏菌8种耐药基因(tetC、tetM、sul1、sul2,aph(3')-Ⅱa、aac(6')-Ⅰb、ant(3')-Ⅰa、aac(3)-Ⅱa)被检测出,而tetA、sul3基因未被检测出。该菌株对复方新诺明、磺胺异恶唑、红霉素、利福平、洁霉素和头孢噻吩6种抗生素耐药,对氟哌酸、氟苯尼考和头孢曲松等8种抗生素敏感。这为治疗小肠结肠炎耶尔森氏菌引起的鱼病积累了资料。  相似文献
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To detect the drug resistance genotype and phenotype produced by Aeromonas veronii isolated from different fish.PCR method was used to detect the aminoglycoside antibiotics resistance genes (aac (3)-Ⅱa, aac (6')-Ⅰb, ant (3")-Ⅰa, aph (3')-Ⅱa), sulfonamides resistance genes (Sul1, Sul2, Sul3) and tetracyclines resistance genes (tetA, tetC, tetM) in isolates. The drug susceptibility to seven Aeromonas veronii strains toward 22 kinds of antibiotics were performed with Kirby-Bauer disc diffusion method. The results showed that the detected resistance genes were aac (3)-Ⅱa (71.4%), aac (6')-Ⅰb (85.7%), Sul2 (85.7%) and tetA (28.5%),while the other resistant genes of ant (3")-Ⅰa, aph (3')-Ⅱa, Sul1, Sul3, tetC and tetM were not checked out. The seven Aeromonas veronii isolates were sensitive to Fosfomycin (100%), Polymyxin B (100%), Furazolidone (85.7%) and Ofloxacin (71.4%). They were resistant to Ampicillin (100%), Acetylspiramycin (100%), Trimethoprim/Sulfamethoxazole (85.7%), Sulfisoxazole (85.7%) and Tetracycline (85.7%). Drug resistance genotype had some correlation with the drug resistance phenotype.  相似文献
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为探究鸡源肺炎克雷伯菌的流行性和耐药情况,本试验采集蛋鸡新鲜粪便60份(雏鸡36只/产蛋鸡24只),通过分离培养、VITEK2 Compact生化鉴定、特异性基因PCR扩增和微量肉汤稀释法对分离菌株进行了菌种鉴定、耐药表型、耐药基因以及毒力基因检测。结果显示,从粪便样本中共分离出48株肺炎克雷伯菌;分离菌株对氨苄西林、大观霉素、四环素、氟苯尼考、磺胺异噁唑和复方新诺明表现出高度耐药,耐药率范围为50.00%~100.00%,对奥格门丁、庆大霉素、头孢类和喹诺酮类药物耐药程度较低,耐药率范围为14.58%~27.08%,对黏菌素和美罗培南敏感;75.02%的菌株表现为多重耐药,最高表现为8重耐药,占10.42%。耐药基因和毒力基因检测结果显示,48株肺炎克雷伯菌共检出aadA1、tetA、oqxA、oqxB、blaTEM和qnrB 等6种耐药基因,以及entB、wabG、uge和kfuBC 等4种毒力基因,本试验结果可为临床用药、动物源细菌耐药性监测和健康养殖提供数据支持。  相似文献
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【Objective】 This study was aimed to investigate the drug resistance phenotype and drug resistance gene carrying status of Acinetobacter pleuropneumoniae (APP),a pathogen of porcine contagious pleuropneumia (PCP) in some large-scale pig farms in Guangdong province,and to analyze and compare their correlation,so as to provide theoretical basis for effective prevention and control of PCP.【Method】 The dead pig materials of suspected PCP collected from large-scale pig farms in different regions of Guangdong province from 2019 to 2021 were isolated,identified and sequenced by pathogen isolation and culture,Gram staining,biochemical test,PCR amplification and other methods.Then the drug sensitivity test was carried out on the isolated strains,and their drug resistance genes were detected by PCR to determine their drug resistance phenotype and the carrying rate of drug resistance genes,and the consistency between them was analyzed and compared.【Result】 The isolates needed to be grown in culture plates containing serum and NAD.Gram staining results showed that the isolated bacteria were red,which could be determined as Gram-negative bacteria.Through biochemical test,PCR results and sequencing analysis,20 strains of APP were isolated,and did not show obvious regional characteristics.All strains showed multiple drug resistance,and about 50% of the strains showed 8 or more drug resistance.The drug resistance rates to tetracyclines,sulfonamides,chloramphenicols and macrolides were 77.5%,65.0%,55.0% and 48.8%,respectively.Further analysis showed that the isolated bacteria were mainly resistant to tetracycline,sulfaisoxazole,florfenicol and lincomycin,but sensitive to cefazolin (Pioneer V) and azithromycin.blaCMY, aph(2″)-Ⅰb,sul1,sul2,sul3,tetA,tetB,tetM,tetO,tetR and floR were detected in 23 main drug resistance genes,and the carrying rates were 85%,85%,50%,60%,75%,30%,100%,85%,100%,70% and 80%,respectively.No quinolones,macrolides and lincomycin related resistance genes were detected.【Conclusion】 APP isolated from pigs in Guangdong province showed extensive drug resistance and multiple drug resistance.The drug resistance gene was basically consistent with the drug resistance phenotype,indicating that the carrying of drug resistance gene was one of the main reasons for bacterial resistance to antibiotics,but there might also be other undetected drug resistance genes or new drug resistance mechanisms.  相似文献
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