羊传染性脓疱病研究进展

羊传染性脓疱病主要感染山羊和绵羊,近年来发现其也可感染包括人在内的多种动物,为一种人兽共患病。对羊传染性脓疱病的病原学、流行特点、临床症状、病理变化、诊断及防治等方面进行了综述。     

Variant surface glycoproteins from Venezuelan trypanosome isolates are recognized by sera from animals infected with either Trypanosoma evansi or Trypanosoma vivax

Salivarian trypanosomes sequentially express only one variant surface glycoprotein (VSG) on their cell surface from a large repertoire of VSG genes. Seven cryopreserved animal trypanosome isolates known as TeAp-ElFrio01, TEVA1 (or TeAp-N/D1), TeGu-N/D1, TeAp-Mantecal01, TeGu-TerecayTrino, TeGu-Terecay03 and TeGu-Terecay323, which had been isolated from different hosts identified in several geographical areas of Venezuela were expanded using adult albino rats. Soluble forms of predominant VSGs expressed during the early infection stages were purified and corresponded to concanavalin A-binding proteins with molecular masses of 48–67 kDa by sodium dodecyl sulfate-polyacrylamide gel electropohoresis, and pI values between 6.1 and 7.5. The biochemical characterization of all purified soluble VSGs revealed that they were dimers in their native form and represented different gene products. Sequencing of some of these proteins yielded peptides homologous to VSGs from Trypanosoma (Trypanozoon) brucei and Trypanosoma (Trypanozoon) evansi and established that they most likely are mosaics generated by homologous recombination. Western blot analysis showed that all purified VSGs were cross-reacting antigens that were recognized by sera from animals infected with either T. evansi or Trypanosoma (Dutonella) vivax. The VSG glycosyl-phosphatidylinositol cross-reacting determinant epitope was only partially responsible for the cross-reactivity of the purified proteins, and antibodies appeared to recognize cross-reacting conformational epitopes from the various soluble VSGs. ELISA experiments were performed using infected bovine sera collected from cattle in a Venezuelan trypanosome-endemic area. In particular, soluble VSGs from two trypanosome isolates, TeGu-N/D1 and TeGu-TeracayTrino, were recognized by 93.38% and 73.55% of naturally T. vivax-infected bovine sera, respectively. However, approximately 70% of the sera samples did not recognize all seven purified proteins. Hence, the use of a combination of various VSGs for the diagnosis of animal trypanosomosis is recommended.     

Urinary and Plasma Catecholamines and Metanephrines in Dogs with Pheochromocytoma,Hypercortisolism, Nonadrenal Disease and in Healthy Dogs

Background

Diagnosis of pheochromocytoma (PC) is based on a combination of clinical suspicion, finding an adrenal mass, increased plasma, and urine concentrations of catecholamine metabolites and is finally confirmed with histopathology. In human medicine, it is controversial whether biochemically testing plasma is superior to testing urine.

Objectives

To measure urinary and plasma catecholamines and metanephrines in healthy dogs, dogs with PC, hypercortisolism (HC), and nonadrenal diseases (NAD) and to determine the test with the best diagnostic performance for dogs with PC.

Animals

Seven PC dogs, 10 dogs with HC, 14 dogs with NAD, 10 healthy dogs.

Methods

Prospective diagnostic clinical study. Urine and heparin plasma samples were collected and stored at −80°C before analysis using high‐pressure liquid chromatography (HPLC) coupled to electrochemical detection or tandem mass spectrometry were performed. Urinary variables were expressed as ratios to urinary creatinine concentration.

Results

Dogs with PC had significantly higher urinary normetanephrine and metanephrine : creatinine ratios and significantly higher plasma‐total and free normetanephrine and plasma‐free metanephrine concentrations compared to the 3 other groups. There were no overlapping results of urinary normetanephrine concentrations between PC and all other groups, and only one PC dog with a plasma normetanephrine concentration in the range of the dogs with HC and NAD disease. Performances of total and free plasma variables were similar. Overlap of epinephrine and norepinephrine results between the groups was large with both urine and plasma.

Conclusion and clinical importance

Measurement of normetanephrine is the preferred biochemical test for PC and urine was superior to plasma.     

Pulmonary Vein‐to‐Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease

Background

Early recognition of left‐sided congestive heart failure (CHF) in dogs with degenerative mitral valve disease (DMVD) is important because it influences medical therapy, timing of follow‐up, and outcome.

Hypothesis

Pulmonary vein diameter‐to‐pulmonary artery diameter ratio (PV/PA) measured by echocardiography can predict CHF.

Animals

Ninety‐eight client‐owned dogs, 37 controls, and 61 dogs with DMVD.

Methods

Prospective clinical cohort study. History, physical examination and Doppler‐echocardiography were performed. Dogs were classified as International Small Animal Cardiac Health Council class I, II or III. Congestive heart failure was identified in a subset of 56 dogs based on radiographic findings. The PV/PA was measured in bidimensional (2D) and M‐mode by 2 investigators blinded to the radiologists’ conclusions.

Results

Interobserver coefficients of variation for PV/PA acquisition and measurement were <10%. The PV/PA in control dogs was approximately 1 and increased with class of heart failure. The presence of CHF could be best predicted by measuring PV/PA in 2D echocardiography (cut‐off, 1.7; area under the curve, 0.98; CI, 0.97–0.98; P < .001) with a sensitivity of 96% and a specificity of 91%.

Conclusion and clinical importance

The PV/PA is a simple and reproducible echocardiographic variable that increases with class of heart failure and may help discriminate dogs in CHF from asymptomatic dogs with DMVD. Additional studies are required to determine whether PV/PA might provide additional information in the integrated interpretation of Doppler‐echocardiographic indices of left ventricular filling pressures and could be used for rapid assessment of CHF in dogs in a critical care setting.     

Diagnostic Leaf Nutrient Norms and Identification of Yield-Limiting Nutrients of Mango in Eastern Plateau and Hill Region of India

The leaf nutritional status of mango orchards was assessed using diagnosis and recommendation integrated system (DRIS). The DRIS norms, which showed higher variance and lower coefficients of variation, are found to have greater diagnostic precision. As per DRIS indices, a relative deficiency for magnesium, zinc, and boron corresponding to relative sufficiency for calcium, nitrogen, phosphorus, sulfur, and potassium was detected in 9-year-old mango orchards. For the younger orchards (6–7 year old), the order of requirement of nutrients was found to be calcium > sulfur > potassium > boron > nitrogen > phosphorus > magnesium > zinc. Boron was found as the most yield-limiting elements in all age group of plants. When the DRIS indices were compared on the basis of soil pH, calcium and magnesium were most yield-limiting nutrients below pH 5.5, while nitrogen, zinc, and boron were found to be most limiting above pH 5.5.     

《兽医临床诊断学》教学改革效果分析

对近3年<兽医临床诊断学>教学改革的效果进行了分析,具体包括教学方法改革效果、实践教学改革效果、考核方式改革效果以及其他方面的改革效果,旨在为兽医临床诊断学及其他课程教学提供有益参考.     

2010年7月张家界市连续两次暴雨过程诊断分析

利用常规观测资料和NCEP再分析资料,对2010年7月在张家界市发生的连续2次暴雨过程的环流背景与动力、热力、不稳定条件、水汽条件等进行了诊断分析。结果表明,这次张家界市连续2次暴雨过程与副高的南北摆动密切相关,随着副热带高压的每一次南撤,带动中低层切变线和地面弱冷空气南下,在张家界触发强对流,形成了强降雨天气。高低空急流在暴雨形成中起着重要作用,低空急流不但为暴雨区带来丰富的水汽和良好的水汽辐合,且在暴雨区中低层形成不稳定层结和上升运动,是对流不稳定能量的建立者和不稳定能量释放的触发者;暴雨出现在垂直运动发展旺盛的上空,强的上升运动与强降水对应关系很好;水汽通量散度中心未来的移动方向对暴雨落区具有较好的指示作用。当某地K指数由大变小,同时SI指数由小变大,不稳定能量EK由极小值逐渐变大,有利于该地区对流发生和中尺度系统生成,正是该地区暴雨爆发时间段。使用恩施多普勒雷达观测资料分析组合反射率因子（CR37）产品发现,张家界市连续2次暴雨过程降水表现为积云为主的混合性降水回波特征。