口服盐酸特比萘芬对幼犬血液常规及生化指标的影响

试验表明，盐酸特比萘芬不同饲喂剂量均可导致犬淋巴细胞百分比、中性粒细胞百分比、白细胞数等血常规检测指标异常。盐酸特比萘酚在幼犬肝脏和肾脏中的毒性是慢性累积的，临床应用中推荐使用盐酸特比萘芬剂量为 5 mg/kg.d进行口服，连续给药不能超过 21d。     

黄连中盐酸小檗碱的提取纯化及抑菌活性研究

为了探究盐酸小檗碱在植物病原真菌方面的抑制作用,以黄连根粉为材料,采用酸水法提取和乙醇重结晶获得盐酸小檗碱纯品,采用生长速率法研究了盐酸小檗碱对常见植物病原菌的抑制活性。结果表明,盐酸小檗碱的提取率为3.3%,经高效液相色谱法测得其含量为93.56%;抑菌活性试验表明,盐酸小檗碱在100μg/mL时对玉米大斑病菌的抑制率达99.9%,EC_(50)为8.20μg/mL,表现出比药剂对照噁霉灵和百菌清更高的抑制活性;进一步的试验表明,与噁霉灵及百菌清相比,盐酸小檗碱对玉米大斑病菌的抑制作用具有持效期长的特点。     

高效液相色谱-串联质谱法测定猪毛中盐酸可乐定和盐酸赛庚啶

建立了高效液相色谱-串联质谱法测定猪毛中盐酸可乐定和盐酸赛庚啶的方法。猪毛样品用0.1 mol/L NaOH溶解后,用0.1 mol/L HCl溶液调节pH值至7.0,经乙腈提取后用UPLCMS/MS进行分析。分析时采用Waters BEH C18色谱柱(100 mm×2.1 mm,1.7μm),以0.10%甲酸水/甲醇作为流动相进行梯度洗脱,电喷雾正电子(ESI~+)模式电离,多反应监测(MRM)模式检测,外标法进行定量。结果表明:盐酸可乐定和盐酸赛庚啶标准溶液在1.0~50μg/L范围内,峰面积与含量呈线性相关。盐酸可乐定和盐酸赛庚啶的检出限均为0.20μg/kg,样品中添标平均回收率在85%~110%之间,相对标准偏差(RSD)小于10%。     

Optimization of Stachydrine Hydrochloride in Leonurus Extraction Method by HPLC-ELSD

The study was aimed to extract stachydrine hydrochloride in leonurus by HPLC-ELSD,and establish hydrochloride stachydrine determination methods for providing theoretical basis of leonurus extraction process optimization.The chromatographic conditions:venusil HILIC hydrophilic column;Mobile phase was acetonitrile -0.2% acetic acid solution (volume ratio 80:20);The flow rate was 1.0 mL/min;And the column temperature was 30 ℃;The detector was ELSD and drift tube temperature was 80 ℃ with the atomization chamber temperature 50 ℃ and carrier gas volume flow 1.0000 mL/min.The results showed that there was a good linear relationship when the stachydrine hydrochloride content was 0.49 to 4.91 μg (R²=0.9993);There was the highest rate of active ingredients extraction of when the extraction time was 1 h,material to water ratio was 20:1 and extracted for 2 times.Combined with production practice,the final optimization scheme was that the extraction time was 1.5 h,material to water ratio was 20:1 and extracted for 2 times.In summary,the determination of stachydrine hydrochloride in leonurus should use HPLC-ELSD method which had high specificity,good stability,response and reproducibility and easy to handle.This method could provide the theoretical basis for optimizing the leonurus extraction process.     

Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA

A detection method of dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection,and asragulus polysacharin injection was established by UPLC-PDA.The Waters ACQUITY UPLC^® BEH C18 column(2.1 mm×50 mm,1.7 μm) was used,the mobile phase consisted of 0.75% triethylamine phosphatic liquor/solution (pH 3.0)-methyl alcohol-acetonitrile(50:45:5),the flow rate was 0.3 mL/min,the column temperature had remained 25℃,photodiode array detector wavelength range was 190 to 400 nm,the recorded wavelength was 242 nm,the injection volume was 10 μL.The linear range of dexamethasone sodium phosphatel was 10.0 to 200.0 mg/L (R²=0.9999),the mean recovery and RSD for dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection and asragulus polysacharin injection were 97.54% and 1.14%,101.59% and 0.19%,100.92% and 0.92%,99.82% and 0.73%,100.08% and 0.62%,respectively.This method was accurate,easy and fast,it was suitable for detection of dexamethasone sodium phosphatein houttuynia injection and other four kinds of injections.     

RP—HPLC法同时测定白头翁汤中盐酸小檗碱、盐酸药根碱、盐酸巴马汀的含量

目的：建立反向高效液相色谱方法同时测定白头翁汤中盐酸小檗碱、盐酸药根碱、盐酸巴马汀的含量。方法：色谱柱为XPODS—AC。。（250minx4．6mm,5I．Lm）;采用线性梯度洗脱方法,流动相：溶液A为乙腈,溶液B为O．05mol／L磷酸二氢钾（磷酸调pH值至3．0）。0—40min溶液A：溶液B为5：95—45：55;流速：1．0mL／min;柱温：室温;检测器：DAD检测器;检测波长：340nm;进样量：10μL。结果：盐酸小檗碱、盐酸药根碱和盐酸巴马汀分别在62．5-l000μg／mL、46．875—750μg／mL和37．5—600μg／mL范围内线性良好;平均加样回收率分别为99．22％（RSD为3．25％）、98．46％（RSD为1．99％）和99．20％（RSD为1．69％）。结论：本方法简便、灵敏、准确,适用于白头翁汤的质量控制。     

Metabolism mediated interaction of α-asarone and Acorus calamus with CYP3A4 and CYP2D6

The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC₅₀ values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC₅₀ values (46.84 ± 1.83-32.99 ± 2.21 μg/ml) comparing to the standard inhibitors and lower IC₅₀ value than α-asarone (65.16 ± 2.37-42.15 ± 2.45 μg/ml).     

盐酸吡利霉素治疗奶牛乳房炎临床药效学及对乳品质影响的研究

本研究依据对黑龙江省部分牧场奶牛乳房炎致病菌分离鉴定以及药敏试验结果,选取盐酸吡利霉素、头孢噻呋钠和复方阿莫西林为试验药物,对患有隐性乳房炎的奶牛进行治疗并对乳品质进行跟踪监测。结果表明盐酸吡利霉素对隐性乳房炎的治愈率最高,对临床型乳房炎的治疗效果略差于复方阿莫西林,盐酸吡利雷素与复方阿莫西林对两种类型乳房炎治疗的总有效率相当,但盐酸吡利霉素对乳品质（尤其是降低体细胞数方面）的改善作用显著好于复方阿莫西林治疗组。     

Photostabilization of biocontrol agents by berberine

We studied the photostabilizing effect of the isoquinoline alkaloid berberine on the following three model photounstable biocontrol agents: the bacterium Bacillus thuringiensis ( Bt ), the baculovirus Spodoptera littoralis nucleopolyhedrosis virus ( SlNPV ), and the entomopathogenic fungus Beauveria bassiana . The photoprotection provided by berberine was evaluated following various periods of simulated sunlight-UV irradiation. Insecticidal activity of Bt and SlNPV , as well as the rate of B. bassiana spore germination served as parameters for the photostabilizing effects. Only a berberine- Bt complex is effective, as practically no photostabilization was evident by just mixing the two components. The UV-sensitive SlNPV and B. bassiana spores were considerably photostabilzed by berberine. The role of the cationic nature of the berberine molecule and the importance of its absorbance spectrum in photostabilization of biocontrol agents are discussed.     

曙红Y褪色光度法测定盐酸普罗帕酮

在pH=3.2的HCl-NaAc缓冲溶液中,曙红Y(EY)与盐酸普罗帕酮(PPF)形成1∶1的离子缔合物,导致溶液的吸收光谱变化,在518 nm处产生明显的褪色作用,并在550 nm处出现新的吸收峰.PPF的质量浓度在0.2～4.0 μg/mL范围内与褪色波长处的吸光度变化值呈良好的线性关系,据此建立一种测定PPF的分光光度新方法.方法简便快捷,灵敏度较高,摩尔吸光系数(ε)为4.16×104 L/(mol·cm).研究了适宜的反应条件和影响因素,并考察了共存物质的影响,表明方法具有较好的选择性,可用于PPF片剂含量测定.