杏鲍菇秋葵咀嚼片配方优化与质量标准研究

为了优选杏鲍菇秋葵咀嚼片配方,以杏鲍菇粗多糖、杏鲍菇超细粉为主要原料,采用全粉末直接压片技术,选取片重差异、崩解时限、硬度及脆碎度的综合评定值为评价指标,通过响应面法确定最佳配方,并制定产品的质量指标。结果表明,杏鲍菇秋葵咀嚼片的最佳配方为杏鲍菇粗多糖38%、杏鲍菇超细粉49%和黄秋葵超细粉13%,制成的咀嚼片表面光滑、色泽均匀。综上,采用多指标综合评分法优化杏鲍菇秋葵咀嚼片配方是可行的,所得工艺配方稳定可靠,这为我国咀嚼片剂工艺创新及其产业化应用研究提供了新思路。     

结石通片对小鼠的急性毒性试验及其对乙二酰胺致大鼠肾结石的作用研究

[目的] 研究结石通片对小鼠的急性毒性作用,对健康大鼠的利尿作用,以及对乙二酰胺致大鼠肾结石的调节作用。[方法] 取ICR小鼠40只,雌、雄各半,随机分为对照组与给药组,每组20只,观察结石通片给药前后小鼠的体重变化和死亡情况;另取SD大鼠36只,雌、雄各半,分为对照组、低剂量组（给予5%结石通片溶液）和高剂量组（给予10%结石通片溶液）,统计各组大鼠24 h排尿量;并采用42只雄性SD大鼠,考查结石通片对给予乙二酰胺（2%掺喂）后大鼠体重、肾重和肾重比的影响。[结果] 给予结石通片溶液后,连续观察7 d,小鼠的各项生理活动未见明显异常,给药组与对照组相比体重差异不显著（P>0.05）;与对照组比较,给予结石通片溶液后大鼠24 h内排尿量明显增加（P<0.01）;与对照组比较,给药组随着给药剂量的增加,结晶和结石的数量极显著（P<0.01或P<0.001）减少,形成结石的动物数量极显著（P<0.01或P<0.001）减少。[结论] 结石通片对小鼠无明显急性毒性作用,对大鼠利尿作用明显,并对乙二酰胺致大鼠肾结石具有明显抑制作用。     

狗尾草压缩特性试验研究

目前国内的压捆机生产出的草产品密度还比较低,向高密度发展是一个必然的发展趋势。为此,通过对狗尾草的高密度压缩试验,研究其压缩过程的基本规律,获得了体积模量与压缩密度、压力与压缩密度及压缩密度与压缩量之间的变化规律,旨在为压缩设备及压缩工艺的优化提供理论依据。     

不同剂量阿苯达唑治疗鼠旋毛虫病效果初探

使用不同剂量阿苯达唑对人工感染旋毛虫的小白鼠进行疗效试验,观察其不同剂量的杀虫效果.结果表明：应用阿苯达唑20 mg/（kg·d）×7治疗鼠旋毛虫病有很好的治疗效果,为阿苯达唑临床应用提供科学参考.     

Albendazole sulphoxide enantiomers in pregnant rats' embryo concentrations and developmental toxicity

Three single oral doses (8.5, 10, and 14 mg/kg) of a racemic formulation of albendazole sulphoxide (ABZSO) were administered to pregnant rats on day 10 of gestation. Mother plasma and embryo concentrations of ABZSO enantiomers and albendazole sulphone (ABZSO(2)) were determined 9 h after administration. The (-)-ABZSO enantiomer showed higher peak concentrations in both maternal plasma and embryo than the (+) enantiomer. An increase in embryo concentrations of ABZSO enantiomers and ABZSO(2) was only observed when dose rose to 14 mg/kg. There was an increase in resorption when the dose increased, but significant differences were only found in the higher dose group when compared with the other groups. The incidence of external and skeletal malformations (mostly of the tail, vertebrae and ribs) rose significantly in the 10 mg/kg group, producing almost 20% and 90% of malformed fetuses, respectively, and gross external and skeletal abnormalities in the thoracic region and limbs were also found.     

紫外分光光度法测定阿苯达唑片含量的不确定度评定

对紫外-可见分光光度法测定阿苯达唑片含量的不确定度进行评定以及测量过程中各种不确定度来源进行分析与评估,含量测定结果可表示为（95．7±2．0）％（k=2）。结果表明,仪器的吸光度、重量差异、称样量和移液管的体积引入的不确定度为测量结果不确定度的主要影响因素。     

高效液相色谱法测定银翘片中牛蒡苷含量的方法建立

为完善银翘片质量标准,采用高效液相色谱法对银翘片中牛蒡苷的含量测定进行了研究。色谱柱为C18（4．6mm×250mm,5μm）,流动相为乙腈-0．1％冰乙酸溶液（25：75）,检测波长为280nm,流速1mL/min,柱温25℃,进样量10μL。牛蒡苷进样浓度在0．1～2mg／mL范围内与峰面积呈良好的线性关系（r=0．9999）;样品平均回收率为96．5％（n=6）,RSD为1．3％。本方法简便、准确度高、重复性好,为进一步控制银翘片的质量提供了依据。     

草莓奶片配方的优化研究

本文是在原味奶片的基础上添加了草莓粉、草莓香精、柠檬酸和食用色素等原料，并通过单因素实验、正交实验和感官评定的方法确定草莓奶片的最佳产品配方。结果表明最佳配方参数为草莓果粉添加量为20‰、草莓香精添加量为3．5‰、柠檬酸添加量5．0‰、食用色素添加量0．0025‰。     

Influence of Nao Xue Kang tablet on dynamic expression of PAR-1 after acute intracerebral hemorrhage in rats

AIM: To study the dynamic expression of protease-activated receptors-1 (PAR-1) after intracerebral hemorrhage (ICH) and the influence of Nao Xue Kang Tablet (NXKT) on it's expression. METHODS: 72 Wistar rats were divided into normal group, ICH model groups (ICH, 6 h, 24 h, 3 d, 7 d), Nao Xue Kang groups (NXKT, 6 h, 24 h, 3 d,7 d). ICH models were produced with the induction of collagenase typeⅦ-S, except normal group. Immunohistochemical method was used to detect PAR-1 protein and RT-PCR technique was used to detect PAR-1 mRNA in brain tissues around the haematoma at different time points of different groups. RESULTS: PAR-1 protein and mRNA were mildly positive in normal group. In ICH model groups, intensity of PAR-1 expression started to increase at 6 h, and further increased at 24 h. PAR-1 expression reached the peak at 3 d and began to descend. At 7 d the decent was obvious. At 6 h, 24 h, 3 d, and 7 d time points, the PAR-1 protein positive cell number and PAR-1 mRNA absorbance ratio in ICH model and NXKT groups were significantly higher than those in normal group (P<0.05 or P<0.01). The PAR-1 protein positive cell number and PAR-1 mRNA absorbance ratio in NXKT group were significantly lower than in ICH model group (P<0.05 or P<0.01). CONCLUSION: After ICH, PAR-1 is continuously activated because of the stimulation of thrombin. Action of thrombin after ICH may be mediated by PAR-1; NXKT may inhibit the activation of PAR-1, so the praxiology is improved. This may be one of the main mechanisms that NXKT could facilitate the recovery of nervous function.     

甲苯咪唑及丙硫苯咪唑对大白鼠不同感染期的旋毛虫肌幼虫杀虫效力

试验采用甲苯咪唑、丙硫苯咪唑单独给药及联合给药,以300ppm混入饲料连续饲喂的方式,对105只人工感染旋毛虫的大白鼠,在不同感染期采用长短不同的给药时间。结果表明:不同感染期的旋毛虫肌幼虫以甲苯咪唑(150ppm)与丙硫苯咪唑(150ppm)联合给药疗效最佳,其次为甲苯咪唑。较经济的给药时间为:感染期在40～90天之间,横纹肌感染量为2343条/克,给药10天;感染期在90～165天之间,横纹肌感染量为2080条/克,给药15天,感染165天以上,横纹肌感染量为2080条/克,给药20天。