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1.
Comparison of effects of dietary‐specific fatty acids on growth and lipid metabolism in Nile tilapia
Yan Liu Jia‐Jing Wen Li‐Jun Ning Jian‐Gang Jiao Fang Qiao Li‐Qiao Chen Mei‐Ling Zhang Zhen‐Yu Du 《Aquaculture Nutrition》2019,25(4):862-872
The dominant fatty acids (FAs) in oils are often used to explain different nutritional effects of dietary oils in fish. However, the amounts of dominant FAs among oils are different, and the nutritional roles of these important FAs in fish have not been precisely compared at similar levels in feeding trials. In the present study, different amounts of palmitic acid were added to safflower oil (SO), olive oil (OO) and fish oil (FO) to obtain comparable amounts (about 550 g/kg of total FAs) of 18:2n‐6, 18:1n‐9 and 20:5n‐3 + 22:6n‐3 and subsequently fed to Nile tilapia (11.1 ± 0.01 g) for 8 weeks. The results showed similar growth among groups but FO group obtained lower fat deposition, serum ALT and AST activities, compared to OO. Lipogenesis‐related gene expressions were higher in OO group than FO group in liver, muscle and adipose tissue, but there were only few differences in these genes between SO and FO groups. Lipid catabolism genes in FO group were higher than OO and SO groups in adipose tissue, but not in muscle, and the significantly higher expressions of CPT1b and PPARα were only observed in liver. Overall, dietary 18:2n‐6, 20:5n‐3 and 22:6n‐3 were beneficial to normal growth and lipid metabolism, whereas high amount of 18:1n‐9 induced lipid deposition and liver damage in Nile tilapia. 相似文献
2.
为了比较饲料中添加鱼油、茶籽油、亚麻籽油以及碘酸钾对中华鳖()稚鳖生长和脂类代谢的影响,本研究配制了4种油脂含量为10%的饲料,分别为鱼油料(FO,对照组)、鱼油+碘酸钾料(FO+PI,碘酸钾添加量为75 mg/kg)、茶籽油料(TO)和亚麻籽油料(LO),饲喂初重为(5.06±0.05)g的中华鳖66 d。FO+PI组和LO组中华鳖稚鳖的存活率显著低于FO组(<0.05)。各组中华鳖稚鳖血浆中葡萄糖、总胆固醇和甘油三酯水平无显著差异(ACACA)的表达水平,上调了中华鳖-499的表达水平(-23b的表达水平(<0.05)。FO+PI组和TO组的稚鳖肝脏细胞内脂滴空泡较少,同时TO显著影响了肠道组织的黏膜褶。结论认为,相比于鱼油组,在饲料中添加10%茶籽油、亚麻籽油和添加75 mg/kg碘酸钾不会引起稚鳖生长差异,但是亚麻籽油和碘酸钾降低了稚鳖的存活率,茶籽油和碘酸钾影响肝脏的脂类代谢。 相似文献
3.
Dietary Lipid Levels Affect the Growth Performance,Lipid Deposition,and Antioxidative Capacity of Juvenile Scaleless Carp,Gymnocypris przewalskii,on the Qinghai‐Tibetan Plateau 下载免费PDF全文
Qiwei Qin Yanlin Tong Ruman Zhu Guoqian Xu Yuan Shi Jianquan Shi Rui Ma 《Journal of the World Aquaculture Society》2018,49(4):788-797
A 56‐d growth trial was conducted to evaluate the growth performance, lipid deposition, and antioxidative capacity of juvenile scaleless carp, Gymnocypris przewalskii, on Qinghai‐Tibetan Plateau. One‐year‐old juveniles (initial weight: 15.99 ± 0.02 g) were fed practical diets with different lipid levels of 4, 5.5, 7, 8.5, and 10%, respectively. Results showed that the best specific growth rate (SGR), feed conversion rate, and total antioxidative capacity (T‐AOC) in hepatopancreas were observed in fish fed the diet with 7% lipid level. Fish fed with high lipid diets (8.5 and 10%) had significantly higher condition factor and viscerosomatic index as well as lipid in muscle, intestine, mesenteric fat tissue, and whole body. Highest level of hepatopancreas lipase and lipoprotein lipases activities as well as malondialdehyde content and the lowest level of feed intake and T‐AOC content in hepatopancreas and intestine were also observed in fish fed with high lipid diets (8.5 and 10%) (P < 0.05). In conclusion, based on SGR and hepatopancreas T‐AOC content, dietary lipid requirement of juvenile G. przewalskii was estimated to be 7.35 and 7.39%, respectively. 相似文献
4.
M. Torfi Mozanzadeh V. Yavari J.G. Marammazi N. Agh E. Gisbert 《Aquaculture Nutrition》2017,23(3):470-483
Four isonitrogenous diets containing different carbohydrate:lipid (CHO:L) ratios (0.3, 0.6, 1.1 and 1.8) were tested in triplicate groups (16 fish per replicate) of silvery‐black porgy juveniles for 8 weeks. Growth performance was not affected by different dietary CHO:L ratios (P > 0.05); however, the viscerosomatic index, the intraperitoneal fat, whole‐body lipid, energy and n‐3 long chain polyunsaturated fatty acids levels increased with decreasing dietary CHO:L ratios (P < 0.05). Fish fed with 1.8 CHO:L diet had the lowest apparent digestibility coefficients of protein and lipid, as well as the lowest plasma haemolytic and lysozyme activities (P < 0.05). Red blood cell counts and plasma glucose levels were higher in fish fed with 1.1 and 1.8 CHO:L ratio diets than in the other groups (P < 0.05). Plasma alkaline phosphatase and alanine aminotransferase, as well as the level of lipid peroxidation and total antioxidant capacity in the liver and plasma increased as dietary CHO:L ratios decreased (P < 0.05). The results of the current study indicated that the diets with CHO:L ratios between 0.6 and 1.1 are optimal for silvery‐black porgy, whereas higher ratios may result in hyperglycaemia and immune suppression, and lower CHO:L ratios may lead to oxidative stress and liver dysfunction. 相似文献
5.
Deyan Stratev Teodora Popova Georgi Zhelyazkov Ivan Vashin Lilko Dospatliev Elitsa Valkova 《Journal Of Aquatic Food Product Technology》2017,26(7):871-879
The study was designed to assess the seasonal variations in the quality and lipid profile of mussel meat (Mytilus galloprovincialis) harvested along the Bulgarian coast of the Black Sea. The trial period lasted from June to October, and the sampling was carried out in the area of Varna Bay. Technological quality of the mussels was determined by measuring the water holding capacity (WHC) and cooking and roasting losses. Proximate composition, total aerobic plate count, and fatty acid analysis were carried out. Technological parameters and nutritional quality of the mussel meat were strongly affected by the season. During the summer, the mussels had higher mass, meat content, and meat yield (p < 0.001) compared to the early autumn, which corresponds to the lowest protein content during this season. Lipid profile showed seasonal variations in both individual and total fatty acid amounts (p < 0.001). Saturated fatty acids (SFA) had the highest content in summer, while monounsaturated fatty acids (MUFA) decreased gradually until the autumn. The content of polyunsaturated fatty acids (PUFA) reached its peak in October, contributing to the most favorable values of the nutritional indices of lipids in the early autumn. 相似文献
6.
Effects of dietary protein to energy ratios on growth,body composition and digestive enzyme activities in Chinese mitten‐handed crab,Eriocheir sinensis 下载免费PDF全文
Yanyan Cui Qianqian Ma Samwel Mchele Limbu Zhenyu Du Nannan Zhang Erchao Li Liqiao Chen 《Aquaculture Research》2017,48(5):2243-2252
This study aimed to determine the optimal protein to energy ratio (P/E ratio) and evaluate the effect of dietary protein and lipid levels on growth performance, body composition and digestive enzymes activities in Chinese mitten‐handed crab, Eriocheir sinensis. Nine practical diets containing three levels both for protein (DP 30%, 35% and 40%) and lipid (DL 2%, 7% and 12%) with P/E ratios ranging from 13.69 to 19.79 mg KJ?1 were fed to four replicates of crabs (3.39 ± 0.10 g) for 10 weeks. Weight gain increased significantly with the increase in DP level at each DL level. Moreover, weight gain increased in crabs fed with diets containing DL level from 2% to 12% and DP level from 30% to 35%. However, the diet containing 40% DP and 12% DL levels significantly decreased the growth performance and protein efficiency of the crabs. The whole crab and hepatopancreas lipid contents also increased as dietary lipid increased, but not dietary protein. The total protease activity increased significantly with the increase in dietary protein at each lipid level. The lipase activity was statistically comparable among different DL levels at each DP level. Taken together, the crab fed the diet containing 35% protein and 12% lipid levels with P/E 15.77 mg KJ?1 revealed optimal growth, feed utilization efficiency and digestive enzymes activities. Moreover, our study indicated that the higher dietary lipid level at a relatively lower dietary protein level could provide protein sparing effect in Eriocheir sinensis. 相似文献
7.
以辽宁盘锦光合蟹业有限公司养殖基地为试验场所,研究施以3种肥料(化肥、鸡肥、化肥与鸡肥的混和肥)对土池培育小球藻的主要营养指标——脂质含量和脂肪酸组成的影响。试验结果表明,施混合肥所培养的小球藻,其藻细胞密度略高于化肥组和鸡肥组;混合肥组小球藻的脂质含量(鲜质量的5.36%)也高于化肥组(鲜质量的4.02%)和鸡肥组(鲜质量的2.89%);混合肥组小球藻的多不饱和脂肪酸和高度不饱和脂肪酸的含量显著优于单一施化肥或鸡肥的小球藻。因此,在土池中施混合肥更有利于培育出高质量的小球藻。 相似文献
8.
J.T. Wang T. Han X.Y. Li Y.X. Yang M. Yang S.X. Hu Y.D. Jiang S. Harpaz 《Aquaculture Nutrition》2017,23(5):994-1002
An 8‐week feeding trial was conducted to assess dietary protein and lipid levels on growth performance, feed utilization and body composition of juvenile red‐spotted grouper (7.85 ± 0.03 g fish?1). Nine semi‐purified diets were formulated containing varying protein levels (440–520 g kg?1, dry matter) and lipid levels (60–120 g kg?1, dry matter). The weight gain of juvenile Epinephelus akaara was affected by dietary protein (p = .005) and its interaction with dietary lipid (p = .020). Viscerosomatic index, intraperitoneal fat ratio and whole‐body lipid level increased with increasing dietary lipid level (p < .001). Nitrogen retention was not affected by dietary protein and lipid, while lipid retention decreased with increasing dietary lipid level (p < .001). The plasma blood urea nitrogen increased with increasing dietary protein level (p = .003). This study showed that diet with 520 g kg?1 protein and 60 g kg?1 lipid with 30.58 mg kJ?1 P:E provided a maximal growth for this species. Moreover, an increase in dietary lipid levels (from 60 to 90 g kg?1) could reduce the protein requirement (from 520 to 480 g kg?1) without affecting the growth performance, while higher fat deposition was observed in fish fed high‐lipid diets. 相似文献
9.
饲料脂肪水平对俄罗斯鲟幼鱼生长、血液生化指标及抗氧化性能的影响 总被引:2,自引:0,他引:2
以鱼油、豆油、亚麻籽油等比例混合,配成脂肪含量分别为5.45%(L1)、7.00%(L2)、10.44%(L3)、14.37%(L4)、16.34%(L5)、18.91%(L6)及20.15%(L7)的等氮饲料,饲喂俄罗斯鲟(Acipenser gueldenstaedtii)幼鱼[(6.20±0.02)g]8周。实验结果表明,L3组的终末体质量、增重率最大,但与L2组间无显著差异(P0.05),L3组的蛋白质效率显著高于其它组(P0.05),且饲料系数最低。L6和L7组终末体质量、增重率和蛋白质效率显著低于L2和L3组(P0.05)。将幼鱼的增重率与饲料脂肪水平进行回归分析,得出俄罗斯鲟幼鱼在饲料脂肪水平为10.69%时生长最佳。随着饲料脂肪水平的升高,各组鱼的肝体比和体粗脂肪有上升趋势,其中肝体比在饲料脂肪含量大于14.37%时显著增大(P0.05)。血清中的甘油三酯、胆固醇含量也随脂肪水平升高而显著提高(P0.05),提示脂肪过高会影响俄罗斯鲟幼鱼的生长,降低蛋白质效率,导致脂肪在体内的过量积蓄。L7组鱼血清中高密度脂蛋白胆固醇含量最高,而L1组鱼的低密度脂蛋白胆固醇含量最高。肝脏脂蛋白酯酶活性随饲料脂肪水平的提高而升高,脂肪酸合成酶活性则随脂肪水平升高而降低,提示饲料脂肪水平的提高会促进鱼体脂肪的分解代谢。肝脏丙二醛含量和超氧化物歧化酶活性均随着饲料脂肪水平的提高显著升高(P0.05),表明脂肪含量过高会导致脂质过氧化,引发机体抗氧化酶活性的升高。综合俄罗斯鲟幼鱼的生长、血液指标及抗氧化性能,建议生产中将幼鱼的饲料脂肪水平控制在7.00%~10.69%。 相似文献
10.
Influence of dietary lipid on growth performance and some lipogenesis‐related gene expression of tongue sole (Cynoglossus semilaevis) larvae 下载免费PDF全文
Yuhui Yuan Songlin Li Lu Zhang Kangsen Mai Wei Xu Qinghui Ai 《Aquaculture Research》2017,48(3):767-779
A 30‐day feeding trial was conducted to investigate the effects of dietary lipid levels on growth performance, activities of digestive enzymes, fatty acid composition and some lipogenesis‐related gene expression of half‐smooth tongue sole (Cynoglossus semilaevis) larvae. Five isoproteic diets were formulated with graded lipid levels (6.68%, 9.84%, 13.47%, 17.89% and 21.88% dry weight) using fish oil as the main lipid source. Each diet was randomly allocated to triplicate groups of 150 larval tongue sole (35 DAH, 54 ± 1 mg). Fish were fed five times daily to apparent satiation during the feeding experiment. Results showed that, the survival rate (SR) of larvae increased significantly firstly, and thereafter decreased significantly. The specific growth rates (SGR) of larvae fed the diet with 13.47% lipid were significantly higher than other treatments. Larvae fed 9.84% or 13.47% dietary lipid showed higher trypsin, lipase, leucine aminopeptidase and alkaline phosphatase (AP) activities than other treatments, whereas amylase activity nearly showed reverse trend with them. The fatty acid composition of the tongue sole larvae was well correlated with dietary fatty acid profile. Expression of acetyl‐CoA carboxylase alpha (ACC1) was found to be slightly negatively correlated with dietary lipid level, and high dietary lipid level depressed the expressions of acetyl‐CoA carboxylase beta (ACC2) and fatty acid synthase (FAS) mRNA expression significantly, implying that larvae may cope with high dietary lipid mainly through down‐regulating lipogenesis‐related gene expression of FAS and ACC2. Besides, on the basis of SGR, the optimal dietary lipid level for larval tongue sole was estimated to be 13.56% using second‐order polynomial curve. 相似文献