全文获取类型
收费全文 | 239篇 |
免费 | 49篇 |
国内免费 | 7篇 |
专业分类
农学 | 1篇 |
5篇 | |
综合类 | 29篇 |
农作物 | 2篇 |
水产渔业 | 100篇 |
畜牧兽医 | 156篇 |
植物保护 | 2篇 |
出版年
2023年 | 2篇 |
2022年 | 12篇 |
2021年 | 12篇 |
2020年 | 19篇 |
2019年 | 16篇 |
2018年 | 24篇 |
2017年 | 30篇 |
2016年 | 18篇 |
2015年 | 9篇 |
2014年 | 20篇 |
2013年 | 21篇 |
2012年 | 27篇 |
2011年 | 12篇 |
2010年 | 12篇 |
2009年 | 12篇 |
2008年 | 12篇 |
2007年 | 10篇 |
2006年 | 8篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 6篇 |
2002年 | 1篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1994年 | 1篇 |
排序方式: 共有295条查询结果,搜索用时 18 毫秒
1.
2.
Rubn Alonso Contreras‐Tapia María Elena Castellanos‐Pez S. Nandini Marcela Ivonne Benítez‐Díaz‐Mirn Gabriela Garza‐Mourio 《Aquaculture Research》2020,51(5):2102-2111
The effect of a commercial probiotic (NanoCrusta, Altacrusta, Mexico City, Mexico) on the growth performance of Brachionus calyciflorus Pallas, 1766, was evaluated. In a first approach, probiotics were supplied in four densities (2.0 × 103, 1.1 × 105, 2.1 × 105 and 2.1 × 106 cells/ml), alone and in combination with Chlorella vulgaris (1 × 106 cells/ml). The test rotifer did not grow on the probiotic alone. However, when probiotics + C. vulgaris were added, the maximum densities (Dmax; ind/ml) and population growth rates (r) observed were higher. In the second experiment, probiotics were supplied at five higher densities (2.1 × 106, 4.2 × 106, 8.5 × 106, 1.7 × 107 and 3.4 × 107 cells/ml) with C. vulgaris and a control treatment with only C. vulgaris (probiotic‐free). Treatments supplied with probiotics between 2.1 × 106 and 1.7 × 107 cells/ml showed significantly higher Dmax and r than the control treatment. The results showed a positive effect of probiotic bacteria when supplied with C. vulgaris. The best outcome showed a Dmax 2.16 times and an r 1.63 times higher than the density of the control treatment. Growth rates were higher in the treatments with probiotics compared to the control. We conclude that application of NanoCrusta is feasible to improve B. calyciflorus production, but the effects need to be tested in larger scales. 相似文献
3.
本研究从节瓜根际分离到一株具有防病促生功能的荧光类假单胞菌FP1761。该菌株对部分植物病原真菌和细菌具有拮抗能力,能够解钾、解有机磷和无机磷,可产生氨、蛋白酶、嗜铁素、吲哚乙酸。生物测定表明菌株FP1761可显著促进小麦生长。生理生化、平均核苷酸相似度、16S rDNA和多基因分析将FP1761鉴定为摩拉维亚假单胞菌(Pseudomonas moraviensis)。菌株FP1761基因组草图全长6.12 Mb,(G+C)含量为59.9%,共编码5467个基因序列。将该菌株与种内3个代表性菌株进行泛基因组和核心基因组分析,共产生 4 357个共有基因,菌株FP1761特有基因327个。利用antiSMASH对菌株次生代谢基因簇进行预测,发现其含有8个潜在的次生代谢产物基因簇。其中两个基因簇与嗜铁素pyoverdine合成相关,未见聚酮类合成基因。基因组分析发现,该菌株具有与病原性假单胞菌相似的III型分泌系统,但丢失了效应蛋白调控因子hrpS和转运相关的hrpH和hrpK1基因。对全基因组扫描,菌株FP1761仅保留了病原性假单胞菌的保守效应蛋白AvrE和HopAA1-1。FP1761是目前已发现的唯一具有III型分泌系统的摩拉维亚假单胞菌。本研究表明摩拉维亚假单胞菌FP1761具有潜在的植物防病促生功能,但其III型分泌系统与植物益生互作机制有待进一步解析。 相似文献
4.
5.
Effect of Bacillus cereus as a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia 下载免费PDF全文
Miao Wang Guanbin Liu Maixin Lu Xiaoli Ke Zhigang Liu Fengying Gao Jianmeng Cao Huaping Zhu Mengmeng Yi Deguang Yu 《Aquaculture Research》2017,48(6):3163-3173
We evaluated the effects of Bacillus cereus, as an additive in water and feed, on the gut microbiota and immunological parameters of Nile tilapia (Oreochromis niloticus) fingerlings. Experiments were performed in tanks and net cages respectively. Experiment 1: Tilapia were housed in tanks for 42 days, and B. cereus was added to the water at 1.0 × 104 cfu mL?1 (Treatment 1) and 1.0 ×105 cfu mL?1 (Treatment 2) weekly. For the control, no probiotic was added. Experiment 2: Tilapia were housed in cages for 42 days, and the feed was supplemented with B. cereus at 1.0 × 107 cfu g?1 (Treatment 1) and 1.0 × 108 cfu g?1 (Treatment 2) weekly. For the control, no probiotic was added. Each treatment contained three replicates, with 50 male tilapias per replicate. The fish from the probiotic treatments in both tank and cage experiments had significantly higher serum lysozyme and peroxidase activities than the control. In the cage experiment, alkaline phosphatase and total superoxide dismutase activities in tilapia were significantly higher in probiotic treatments compared with the control. The results of polymerase chain reaction‐denaturing gradient gel electrophoresis showed that B. cereus supplementation in the feed and water affected the autochthonous gut bacteria community of tilapia and stimulated various potentially beneficial bacteria. Therefore, B. cereus, as a water or feed additive, could enhance the immune status and affect the gut microbiota of tilapia. Bacillus cereus was more effective as a feed supplement rather than a water additive for enhancing the immune status of tilapia. 相似文献
6.
Probiotic efficiency of Bacillus sp. in Labeo rohita challenged by Aeromonas hydrophila: assessment of stress profile,haemato‐biochemical parameters and immune responses 下载免费PDF全文
Ankita Nandi Goutam Banerjee Suhas Kumar Dan Koushik Ghosh Arun Kumar Ray 《Aquaculture Research》2017,48(8):4334-4345
Fish are always susceptible to a wide variety of deadly pathogens which cause a huge loss in aquaculture industries. In this investigation, we have demonstrated the in vivo probiotic efficiency of Bacillus sp. MVF1 (GenBank Acc. No. KP256503) in Labeo rohita challenged with pathogenic strain of Aeromonas hydrophila (MTCC 1739). To check the probiotic potential of the selected bacterial strain, fish were divided into four groups: control, D1, D2 and D3. A total of 100 days (70 days probiotic feeding + 71th day sampling and 28 days challenged test + 29th day sampling) of feeding trial was conducted. To establish the probiotic potential of Bacillus sp. MVF1, certain haematological parameters (haemoglobin, total erythrocyte and leucocyte count), serum biochemical parameters (total protein, albumin and globulin), immune parameters (serum lysozyme and total IgM levels) and hepatic stress profile (malondialdehyde production, superoxide dismutase and catalase activity) have been measured. Our results demonstrated that red blood cell number, white blood cell number and haemoglobin content were much higher in D2 group fish compared to other groups and control fish. Similarly, total protein contents, albumin concentration, globulin concentration, lysozyme activity and IgM production were also recorded to be highest in D2 group fish. This finding clearly indicated the probiotic potential of Bacillus sp. MVF1 in L. rohita. Furthermore, our results also demonstrated that 1 × 107 CFU g?1 feed (D2) provides better immunity compared to 1 × 105 (D1) and 1 × 109 (D3). Due to beneficial effects, the bacterium Bacillus sp. MVF1 might be useful in aquaculture industries to reduce the disease susceptibility. 相似文献
7.
The aim of this study was to determine the effects of dietary probiotics on reproductive performance, growth, haematological and biochemical parameters of female rainbow trout broodstock. A total of 60 broodstocks with an average initial weight of 2,267.4 ± 54.9 g were divided into four groups and three replicates in raceway ponds. Fish were fed with diets containing 0 (control), 1 × 109 (P1), 2 × 109 (P2) and 4 × 109 (P3) CFU probiotic per kg diet twice a day for 8 weeks before spawning season. Results revealed that higher levels of dietary probiotics (P3) enhanced protein efficiency ratio and decreased feed conversion ratio (p < .05). There were no significant differences in haematological and other growth parameters among different treatments. According to the results, probiotic‐fed treatments had the lowest levels of cholesterol (237.7 ± 4.3 mg/dl) and the highest levels of total protein (11.3 ± 0.3 g/dl) and albumin (6.9 ± 0.3 g/dl), so that differences were found between P3 and control groups in all cases (p < .05). Based on the observations from reproductive parameters, the highest egg diameter, working and relative fecundities, fertilization and hatching rates, eyed eggs survival and alevins survival rates up to the absorption of the yolk sac were observed in P3 treatment (p < .05). Moreover, eyeing, hatching and yolk sac absorbing stages in P3 treatment were earlier than those in other treatments (p < .05). The results of present study demonstrated that probiotic supplementation improved reproductive performance and some growth and biochemical parameters in female rainbow trout broodstock and the best performance was observed in fish fed 4 × 109 CFU probiotic per kg diet. Due to an increase in egg production and egg size in probiotic‐fed treatments, this feed additive can be used as a suitable ingredient to increase high‐quality egg production in rainbow trout breeders. 相似文献
8.
The feeding trial was conducted to evaluate the potentials of Clostridium butyricum in the diet of tilapia. Fish (~14 g) were fed with basal diet supplemented with 0 (Control), 0.5 (C‐1), 1 (C‐2), 2 (C‐3), 4 (C‐4) and 8 (C‐5) g/kg commercial probiotic‐containing C. butyricum (1.5 × 108 CFU/g) for 8 weeks. The results showed that weight gain significantly increased, and feed conversion ratio decreased in the C‐2, C‐3 and C‐4 groups (p < .05). The protein retention (except C‐1 group), lipid retention and apparent digestibility coefficient (ADC) of dry matter in probiotic supplementation groups were significantly enhanced, and ADC of protein in the C‐4 group was also improved (p < .05). The supplementation of probiotic significantly increased villus height in anterior intestines and reduced the numbers of intestinal Escherichia coli (p < .05). High‐throughput sequencing showed that top three phyla namely Planctomycetes in all probiotic‐containing groups, Proteobacteria in the C‐1 and C‐2 groups and Chloroflexi in the C‐3 group had higher level than the NC group. The cumulative mortality was reduced by dietary probiotic after challenging with Aeromonas hydrophila (p < .05). In conclusion, C. butyricum can be supplemented at 1–2 g/kg feed for promoting the growth, feed utilization, gut health and microbiota of tilapia. 相似文献
9.
Fermenting different raw materials (corn, soybean meal and cottonseed meal) with three kinds of strains (Lactobacillus, yeast and Bacillus subtilis) were employed to optimize the fermentation parameter with sensory evaluation, pH and amount of probiotics retention.The results showed that the time to reach stable pH of materials with different substrates was different.Cottonseed meal had the longest time to reach stable pH followed by soybean meal, while corn had the shortest time under the condition of same water content.Different substrates had no effect on initial pH of corn, soybean meal and cottonseed meal.The count of the probiotics was also influenced by different substrates.When pH reached steady state, corn had the largest number of the probiotics followed by soybean meal and cottonseed meal.The color and smell of three materials after fermented had distinctive features.But cottonseed meal had the highest viscosity followed by soybean meal and corn.Because of shorter time and more microorganisms, corn was better than soybean meal and cottonseed meal to be fermenting medium. 相似文献
10.
为了研究复合益生菌菌液对奶牛产奶量、乳品质量、牛群健康和肠道菌群的影响,选择20头条件相近的健康奶牛随机分为试验组和对照组,采用菌液与饲料混合的方法饲喂牛群,考察其对奶牛产奶量,肠道菌群乳品质量和牛群健康的影响。结果表明,食用复合益生菌液的每头牛比对照组平均每天可以多产2.9kg牛奶,提高产奶量14.8%,差异极显著(P0.01),经济效益显著;复合益生菌菌液可以促进肠道菌群调整,使有益菌-主要是乳酸菌成为优势菌群,并可以抑制霉菌和降解霉菌毒素,降低牛乳中体细胞数,预防隐性乳腺(房)炎。 相似文献