Secondary sexual traits and semen characteristic of chicken germline chimeras

Birds obtained by embryo engineering are used to study embryo development and to produce transgenic birds. As this method of producing birds still generate strong emotions of the public opinion head ornaments, testes and semen characteristics of sex chimera roosters were examined to check whether they differ from chickens obtained by non‐manipulated methods. Measurements of head ornaments, testes and semen were correlated with each other. Semen quality factor (SQF) was calculated, as well as the level of fluctuating asymmetry (FA) of bilateral traits (wattles and testes). Positive correlation was found for comb width and wattle length and comb thickness and sperm concentration. Semen characteristics and FA did not exceed the level encounter in other chicken lines. Results obtained indicate that germline chimeras are similar in appearance of secondary sexual traits, and semen and testes characteristics to chickens produced in non‐manipulated way.     

对公路、桥梁工程质量问题及其预探浅析

本文作者从管理和施工两个方面对公路、桥梁工程质量问题及其预防控制措施进行了较全面的论述。     

负水头条件下的水平一维土壤吸渗特征

该文分析了不同负水头条件下土壤水分运动特征,并通过室内模拟试验研究了不同负水头条件下水平一维土壤的吸渗特征,根据Brooks-corey模型推求了描述负水头条件下土壤吸渗公式,并分析了各参量间的函数关系。分析了Philip吸渗公式在负水头条件下的适用性,并对所建立的公式进行检验。研究结果表明：上述理论关系可以很好描述负水头条件下水平一维土壤吸渗过程。     

稳流器对摇臂式喷头水力损失影响的数值模拟计算

稳流器是喷头内的主要过流部件,喷头内过流部件的压力损失是评价喷头好坏的指标。利用ANSYS/FLOTRAN有限元分析软件,根据喷头内部流体流态,激活标准K-ε湍流模型,对装有不同型式稳流器的PY130型喷头内部三维流场进行数值模拟。经过建模、分网、加载、计算这一流程,对喷头内流场进行微观化分析,得出速度矢量分布图和压力等值分布图,直观地反映了喷管内部流场紊流程度变化和压力变化并可得出喷头水力损失值,为喷头的优化设计提供合理的依据。     

Response of black, cranberry, kidney, and white bean to linuron

Dry bean producers in Ontario, Canada, have few herbicide options available for annual broad-leaved weed management and there is little information on the tolerance of dry bean to linuron. The tolerance of black, cranberry, kidney, and white bean to the pre-emergence (PRE) application of linuron at the rates of 0, 500, 1000, 1500, 2000, and 2500 g ai ha⁻¹ was evaluated in field studies conducted in 2005 and 2006 at Exeter and in 2006 at Ridgetown, Ontario. The four market classes differed in their response to linuron. Cranberry and kidney bean were more tolerant to the PRE application of linuron than black and white bean. Linuron applied PRE caused as much as 12% injury in cranberry and kidney bean, 47% injury in black bean, and 56% injury in white bean. Linuron applied PRE at 500–2500 g ai ha⁻¹ had no effect on the height of cranberry and kidney bean but decreased the height by 7, 8, and 15% in black bean and by 10, 13, and 23% in white bean at 1500, 2000, and 2500 g ai ha⁻¹, respectively. Linuron applied PRE at the rates evaluated did not cause any adverse affect on the yield of cranberry, kidney, and white bean but black bean yield was reduced by 16% at 2500 g ai ha⁻¹. Based on these results, there is not an adequate margin of crop safety for the PRE application of linuron in black and white bean at rates >1000 g ai ha⁻¹. However, there is a potential for the use of linuron PRE for weed management in cranberry and kidney bean at the rates evaluated.     

Repression of deoxynivalenol accumulation and expression of Tri genes in Fusarium culmorum by fungicides in vitro

A defined medium was developed in which to monitor deoxynivalenol (DON) accumulation, fungal growth and expression of genes involved in trichothecene biosynthesis (designated Tri genes). In liquid culture, DON accumulated shortly after maximum expression of Tri6 and coincident with expression of Tri5. This was generally 96 h after inoculation. The effects of sublethal concentrations of the fungicides azoxystrobin, trifloxystrobin, kresoxim-methyl and tebuconazole on biosynthesis of the trichothecene DON by Fusarium culmorum were studied using this medium. The strobilurin fungicides trifloxystrobin and azoxystrobin significantly reduced the accumulation of DON in culture medium at a range of concentrations. Kresoxim-methyl, also a strobilurin, and tebuconazole, a triazole, did not significantly reduce the accumulation of DON, although levels were lower than those in nonamended cultures. Trifloxystrobin significantly reduced the accumulation of DON when added to cultures before initiation of trichothecene biosynthesis. RT-PCR assays of the expression of Tri6 and Tri5 genes indicated that trifloxystrobin acted by inhibiting the initiation of trichothecene biosynthesis.     

金银花尺蠖幼虫头部化学感受器扫描电镜观察

为了解金银花尺蠖Heterolocha jinyinhuaphaga Chu幼虫头部化学感受器的种类、形态、数量和分布,利用扫描电镜技术观察其老熟幼虫头部形态及触角和口器上的化学感受器。结果表明,金银花尺蠖老熟幼虫头部呈椭圆形;触角上着生1个毛形感受器、3个刺形感受器、1个栓锥形感受器和2个锥形感受器;上颚分布有1对毛形感受器;下颚分布有3个毛形感受器、2个刺形感受器、2个栓锥形感受器和8个锥形感受器;下唇须上有1个栓锥形感受器和1个刺形感受器;在吐丝器前方两侧有1对刺形感受器。金银花尺蠖幼虫触角上的化学感受器较少,口器附肢上化学感受器较多。     

玉米丝黑穗病原菌冬孢子萌发条件研究

通过不同条件对玉米丝黑穗病菌冬孢子萌发率影响的试验研究表明,甲醛处理对玉米丝黑穗病菌冬孢子萌发有明显促进作用,萌发率达88.89%,冬孢子在麦芽糖和水琼脂培养基上萌发率最高达84.49%。在不同温度和pH条件下冬孢子萌发率差异较大。冬孢子在28℃时最适合萌发,达89.67%,在pH为7时萌发率最高达89.02%。     

2018年我国小麦赤霉病菌对多菌灵抗性检测

以多菌灵为主的苯并咪唑类杀菌剂一直是小麦抽穗扬花期防控赤霉病的主要手段之一。本研究对2018年我国主要麦区采集的1 464株赤霉病菌菌株进行多菌灵抗性分子检测。共检测出多菌灵抗性菌株97株,抗性频率为6.63%,同时发现抗性菌株以F167Y突变频率最高,其次为E198Q和F200Y。通过比较不同省份间多菌灵抗性发生频率发现,长江中下游麦区赤霉病菌群体抗性频率明显高于黄淮麦区群体。本研究相比之前研究中的抗性频率大幅度上升,表明在多菌灵的选择压力下,多菌灵抗性种群发展迅速。为防止抗性群体的进一步发展,致使多菌灵防治赤霉病失效,应采用混配、复配药剂、不同作用机理的杀菌剂交替轮换使用来防治小麦赤霉病。     

麦冬水提物对猫肾细胞的影响研究

麦冬是我国传统中药之一,兼备保健和治疗作用。为了探究麦冬能否应用在宠物食品中,本试验以不同浓度的麦冬水提物(3.91 ～8000 μg/mL)为受试药物,从细胞的形态、增殖、凋亡及周期4个方面对猫肾细胞(F81)进行安全性评价。结果表明：在试验浓度范围内,麦冬水提物没有抑制F81的增殖,且在3.91～62.5 μg/mL的浓度范围内,凋亡及周期结果经统计学分析均无显著性差异,与对照组接近,且细胞形态良好。说明在一定浓度范围内,麦冬水提物对F81细胞无毒害作用。