Natural bixin as a potential carotenoid for enhancing pigmentation and colour in goldfish (Carassius auratus)

Discovering natural carotenoids for colour enhancement and health benefits of fish is important to develop new feed formulations. We have purified natural bixin from achiote seeds and evaluated the effect of colour enhancing and pigmentation in goldfish. Varying levels of bixin‐based diets were prepared with 420 g kg^?1 of crude protein and 120 g kg^?1 of lipid content. Our results clearly showed that bixin (0.05, 0.10, 0.20 and 0.60 g kg^?1) based diets significantly (P < 0.05) enhanced the skin and fin colour at 30 and 60 days compared to diet without bixin. Interestingly, diet which contains 0.20 g kg^?1 bixin and commercial feed (with astaxanthin) had similar effect on carotenoid deposition in skin. Moreover, total carotenoid deposition in fin was higher than in skin of all bixin‐containing diets. However, 0.60 g kg^?1 bixin‐containing diet had lower specific growth rate (1.01 ± 0.01) and higher feed conversion ratio (2.05 ± 0.19) compared to the control group. The present results demonstrate that achiote bixin can be successfully used as an alternative natural carotenoid source against synthetic astaxanthin in fish feed. Our data indicate that 0.20 g kg^?1 is a suitable dietary level of bixin to ensure strong pigmentation, acceptable growth and feed utilization in goldfish.     

湖南省西南部蝶类资源研究

用采集和饲养相结合的方法收集到湘西南蝴蝶标本293种和亚种，分属11科148属。首次发现湖南新记录143种。一批世界精品蝶，中国珍蝶和在湘西南有分布而全世界仅有1－5种的属。次次列出我省珍稀蝶39种。对湘西南蝶类分布区系及特点进行了详尽分析，同时提出了保护，利用蝶类资源的建议。     

浙西南山区蝴蝶种类调查

经调查和鉴定,在浙西南采集到蝴蝶169种,分别隶属于10科,其中凤蝶科19种,环蝶科3种,粉蝶科12种,眼蝶科30种,蛱蝶科54种,弄蝶科25种,灰蝶科22种,珍蝶科1种,岘蝶科2种,斑蝶科1种.采到浙江新纪录5种,待命名1种,隶属国家保护名录4种.     

空气罐与超压泄压阀联合水锤防护特性

提出了一种空气罐与超压泄压阀联合设置的停泵水锤防护方案,并基于瞬变流计算的特征线法建立了空气罐、液控蝶阀、超压泄压阀等边界条件的数学模型,模拟了停泵工况下系统的压力变化过程.结合工程实例,对比分析了空气罐单独防护、空气罐与两阶段关闭泵后液控蝶阀联合防护以及空气罐与超压泄压阀联合防护对停泵水锤的影响.在空气罐体型一定时,对超压泄压阀的启闭规律进行了敏感性分析.计算结果表明,空气罐与两阶段关闭泵后液控蝶阀联合防护方案对输水系统正负水锤防护均不利;而空气罐与超压泄压阀联合防护方案对输水系统正负水锤均有较好的防护效果,与空气罐单独防护方案相比,泵后高压管段最高压应力由1.343 MPa降至1.087 MPa,在满足承压标准1.2 MPa的基础上安全裕度提高了9.4%,空气罐体型也由200 m³缩减至160 m³.超压泄压阀应在5 s内开启至全开度,且开启后持续时间应接近1个相长.     

Reproductive ability of triploid ornamental (koi) carp (Cyprinus carpio L.) × goldfish (Carassius auratus L.) hybrids and characteristics of their offspring

The purpose of this study was to investigate reproductive ability of backcross triploid koi (Cyprinus carpio L.) × goldfish (Carassius auratus L.) hybrids. These triploids have been obtained by crossing of F₁ hybrid females producing diploid eggs with males of parental species. Triploid hybrid females, when crossed with goldfish or koi males, produced mostly aneuploid fish with ploidy range from approximately 2.2n–3.2n with a mean value 2.5n; some fish in crosses of triploid females with koi males were tetraploid (4.0n). Since analysed fish had in their genomes one haploid set from parental males, the data indicate that triploid hybrid females mostly produced aneuploid eggs with ploidy range from approximately 1.2n–2.2n and a mean ploidy around 1.5n while some eggs were triploid. Triploid hybrid males were completely sterile and have not released any sperm after hormonal injection. Despite their low viability, some aneuploid fish obtained from triploid hybrid females were raised in indoor recirculating systems until the age of 2 years and their reproductive ability has been evaluated. One aneuploid female with ploidy 2.1n produced larvae with ploidy range from 2.9n to 3.4n with a mean ploidy of 3.1n when crossed with a koi male; about 60% of obtained larvae had ploidy from 3.0n to 3.2n. These data indicate that this female produced mostly eggs with unreduced ploidy level.     

西双版纳南览河流域常见观赏蝶种及其寄主植物资源调查

西双版纳勐海县境内南览河流域具有良好的天然植被,蝴蝶种类繁多。为了开展重要观赏蝶种的人工繁育试验,2013—2018年对南览河中低海拔地带的常见观赏蝴蝶及其野生寄主植物资源开展了进一步的深入调查,结果表明,经过5年定点调查和沿线观察,在区域内发现兼具较高观赏价值及人工繁育潜力的蝶种46个;通过观察野外成虫的产卵活动,结合野外套袋及室内饲养幼虫,发现蝴蝶寄主植物38种,初步查明了相关蝶种的野生寄主植物资源。调查工作可为筛选出当地适生优良寄主植物、进一步开展相关蝶种的人工养殖试验提供参考。     

复杂泵系统边界条件建模及水锤计算

本文对复杂泵系统水锤数学模型进行了分析与推导 ,建立了带盲管的多台并联泵向不同阻力特性的多管道供水的水锤计算方程组并对该非线性双曲方程进行了求解 ,对该系统三次水锤事故进行了模拟计算 ,找出了三次事故的原因。     

蝴蝶多样性与地形相关性研究——以大峪流域为例

蝴蝶物种丰富度是蝴蝶多样性研究的热点之一,以大峪流域的蝴蝶为研究对象,借助GIS技术,结合数字高程模型（DEM）数据,研究了大峪流域内的蝴蝶多样性沿海拔梯度分布格局,及其与地形复杂度之间的关系。结果表明：随着海拔的升高,蝴蝶的科、属、种丰富度和密度均呈逐渐降低的趋势;蝴蝶的丰富度和密度与地形复杂度成正相关,随地形复杂度的降低而降低。     

jnk3基因在金鱼和斑马鱼不同发育时期胚胎与成体不同组织中的分化表达

JNKs是丝裂原活化蛋白激酶(MAPK)家族重要成员,参与应激反应和动物体轴的构建。为了进一步了解JNK家族在动物发育中的功能,首次分离和克隆了金鱼jnk3基因,研究了jnk3在金鱼和斑马鱼组织特异性和胚胎发育特异性表达状况。金鱼jnk3基因cDNA总长1 794 bp,其中阅读框1 293 bp,编码430个氨基酸。对jnk3基因序列及其推导的氨基酸序列进行同源性分析,结果显示,jnk3基因在脊椎动物较为保守,金鱼jnk3基因核苷酸序列与斑马鱼、人的同源性分别为94.1%和80.7%,金鱼jnk3基因氨基酸序列与斑马鱼、人的同源性分别为99.7%和93.4%。jnk3基因在鱼类成体中的表达存在着显著的组织差异,在金鱼和斑马鱼脑和精巢组织的表达具专一性,此外在斑马鱼心脏中也检测到jnk3的表达。RT-PCR检测结果显示,在鱼类胚胎发育中,神经胚期开始检测到jnk3基因mRNA表达,从神经胚到心跳期胚胎,jnk3基因表达水平逐渐增高,此后直至出膜一直保持较高的表达水平。研究表明,jnk3基因可能在鱼类后期的胚胎发育和成体脑、精巢与心脏发育中具有重要作用。     

河南省旱灾对称性特征及趋势判断

根据河南省1955-2010年干旱受灾面积历史资料,统计出其发生特大干旱年份起始年的数列。首先,两次运用三元、四元、五元可公度法对河南省旱灾的发生趋势进行预测:第一次预测出2011年会发生特大干旱,结果在2011年确实有干旱存在;第二次把2011年加入原数列,预测出下一次可能发生旱灾的时间。其次,运用蝴蝶结构图法对旱灾的时间对称性进行分析。结果表明:河南省的特大干旱符合可公度特征并存在时间对称性规律,时间间隔以2a、12a、19a最为显著,且2013,2016年左右河南省可能发生特大干旱,概率分别为58.3%、54.4%。