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巢式群体可以利用多个亲本解析复杂性状的遗传机制。本研究利用1个共同亲本与6个基础亲本所配置巢式组合F2:3家系的种子脂肪含量数据,分析了花生脂肪含量的遗传模型,旨在探明不同的基础亲本组合中脂肪含量性状的遗传差异,为制定脂肪含量遗传改良的亲本选配和后代选择策略提供依据。6个组合的共同亲本为高脂肪含量的普通型大果品种豫花15号,其他6个基础亲本为不同脂肪含量和不同植物学类型的品种。结果表明,在不同杂交组合中脂肪含量的遗传模式有所不同,6个组合分别符合无主基因模型、1对主基因加性显性模型和2对主基因等显性模型3种遗传模式。各种遗传效应的估计值也各不相同,主基因遗传力从32%到80%,说明不同杂交组合中,控制脂肪含量的基因位点差异及其重组和分离方式不同。高脂肪含量双亲杂交后代的高脂肪含量个体较多,但主基因遗传力较低,不宜在早代实施表型选择;双亲脂肪含量差异较大的后代脂肪含量变异幅度更大,能够选择到不同脂肪含量的类型。本研究也表明,巢式组合具有较丰富的脂肪含量变异类型,揭示出脂肪含量性状遗传的复杂性和多基因调控的特点,为较全面地了解脂肪含量的遗传提供了基础。该巢式群体也将有助于进一步开展脂肪含量的QTL定位研究。 相似文献
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为明确矿质元素指纹对粉葛产地鉴别的可行性,筛选出鉴别粉葛产地的有效指标,本研究采用电感耦合等离子体质谱仪(ICP-MS)测定陕西、湖北、广西3个地域粉葛和土壤样品中B、Na、Mg、P、K、Ca、Mn、Co、Cu、Zn、Rb、Sr、Mo、Cd、Cs、Ba、La、Ce、Pr、Nd、Sm和Ti 22种矿质元素含量,并结合主成分分析、逐步判别分析和聚类分析对不同地域样品进行分类。结果表明,除B、Cu元素外,其余元素含量在不同地域粉葛间均存在显著差异(P<0.05);不同地域间粉葛矿质元素含量表现出独有的地理指纹特征,经逐步判别分析筛选出Mg、P、Co、Rb和Sr 5种元素,经回代检验和交叉检验的整体判别率分别为97.7%和93.0%;相关性分析结果表明,粉葛中Rb、Sr和Ti元素含量与对应土壤中的元素呈极显著正相关(P<0.01)。因此,矿质元素指纹结合多元统计分析能够在一定程度上实现不同产地粉葛的鉴别,这为粉葛产地溯源的可行性提供了技术支持。 相似文献
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摘 要:【目的】为了保护和发展极小种群植物川柿,开展此研究。【方法】采用观测法,连续3年对其生物学特性研究;种子萌发方法:(1)后熟0、30、45、60天,剥离清洗播种;(2)后熟60天,以GA300、600、1000 mg/L溶液浸种1h;播种基质为河沙(保持一定湿度),放置室内,统计萌发时间和出苗率。【结果】(1)川柿花期4-5月,10月为果熟期,单果直径32.7±5.80mm,单果重23.0±5.30g;种子肾形、千粒重1070g;成年植株呈隔年开花、结果现象;(2)种子经“后熟+河沙播种”,可萌发,其中以后熟60天的出苗率较高,为65%;直接播种种子,出苗率5%;“后熟+GA溶液浸种+河沙播种”,出苗率均高于65%,其中以GA600mg/L浸泡1h处理,出苗率最高为83.3%。【结论】极小种群植物川柿为常绿叶乔木,有隔年开花、结果现象; “果后熟60天+GA600mg/L浸泡种子1h +室内河沙播种”可提高种子萌发率,为川柿种子繁殖提供参考。 相似文献
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为了有效修复日渐退化的雅鲁藏布江中游河谷地区山地植被,采用群落生态学方法对雅鲁藏布江河谷亚高山灌丛草地群落进行了研究分析.结果表明:该区灌丛草地群落共有维管束植物166种,隶属于47科115属,种类组成较为丰富,属内种系相对贫乏.地理成分及科属分析表明,该区灌丛草地群落具有明显的温带性质;生活型和叶的性质分析表明,该区灌丛草地群落的外貌主要是由落叶全缘叶单叶矮高位芽植物所决定的.群落结构简单,仅有灌木层和草本层.雅鲁藏布江河谷半干旱区亚高山灌丛草地群落的总特点是以地面芽、微形叶、草质叶、单叶、全缘叶为特征,反映了青藏高原高山、寒、旱的性质.在分析灌丛草地群落特征的基础上,还对其生态修复进行了探讨. 相似文献
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Lili Guo Dalong Guo Wei Zhao 《The Journal of Horticultural Science and Biotechnology》2018,93(4):416-424
Tree peony (Paeonia suffruticosa Andr.), considered by many to be the national flower of China, is famous for its ornamental, medicinal and culinary attributes. In this study, a total of 8,663 Simple Sequence Repeats (SSRs) were detected by means of a microsatellite search of unigene sequences identified from the de novo assembly of sequence data from different genotypes of tree peony from the Zhongyuan group. Among 100 randomly selected SSR markers, 25 were successfully amplified and showed polymorphism in 31 tree peony accessions. The number of polymorphic alleles ranged from 3 to 11 for each locus and the polymorphism information content value (PIC) ranged from 0.58 to 0.85, with a mean of 0.73, indicating a high level of discriminative capability. To analyse the genetic diversity, a phylogenetic tree was constructed, which demonstrated that tree peonies of similar flower colour were clustered together. The large number of tree peony SSR markers identified in this study will be valuable in studies in genetic diversity and linkage map construction, in gene localisation and cloning and in molecular-marker-assisted breeding in tree peony. 相似文献
8.
Feng-yong GE Na ZHENG Liu-ping ZHANG Wen-kun HUANG De-liang PENG Shi-ming LIU 《农业科学学报》2018,17(12):2734-2744
The resistance of soybean (Glycine max (L.) Merr.) to soybean cyst nematode (SCN, Heterodera glycines Ichinohe), which is a devastating pathogen in soybean production and causes a large quantity of annual yield loss worldwide, can shift during the long-term interaction and domestication. It is vital to identify more new resistance genetic sources for identification of novel genes underlying resistance to SCN for management of this pathogen. In the present study, first, two ethane methylsulfonate-mutagenesis soybean M2 populations of PI 437654, which shows a broad resistance to almost all of SCN races, and Zhonghuang 13, which is a soybean cultivar in China conferring strong resistance to lodging, were developed. Many types of morphological phenotypes such as four- and five-leaflet leaves were observed from these two soybean M2 populations. Second, 13 mutants were identified and confirmed to exhibit alteration of resistance to SCN race 4 through the forward genetic screening of 400 mutants of the PI 437654 M2 population, the rate of mutants with alteration of SCN-infection phenotype is 3.25%. Third, these identified mutants were further verified not to show any changes in the genomic sequences of the three known SCN-resistant genes, GmSHMT08, GmSNAP18 and GmSANP11, compared to the wild-type soybean; and all of them were still resistant to SCN race 3 similar to the wild-type soybean. Taken together, we can conclude that the 13 mutants identified in the present study carry the mutations of the new gene(s) which contribute(s) to the resistance to SCN race 4 in PI 437654 and can be potentially used as the genetic soybean sources to further identify the novel SCN-resistant gene(s). 相似文献
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Hong JIANG Shi SUN Wen-Wen SONG Cun-Xiang WU Ting-Ting WU Shui-Xiu HU Tian-Fu HAN 《作物学报》2018,44(10):1448-1458
In 2014 and 2015, 60 Maturity Group (MG) III soybean varieties from different geographic regions were chosen to characterize the maturity-related traits and to identify the alleles of the maturity genes (E alleles) in these varieties. The MGIII soybean varieties shared the similar growth period but owned different growth period structure. The spring-sowing MGIII soybean varieties from Northern China and the US bloomed earlier (shorter VP) and exhibited lower R/V values than those from Yellow-Huai-Hai River Valley (YHH) and South China. Six combinations of E alleles were identified in the tested Chinese varieties, among them E1e2E3E4 and e1-asE2E3E4 were found to distribute in larger areas of different regions and cover more ecotypes than other four combinations. In contrast, the type of e1-asE2E3E4 was the only E allele combination in the eight US standard varieties, indicating that the Chinese MGIII varieties were more abundant in genetic variations for the maturity-related traits. By comparing the average effect of each E allele on the maturity-related traits in MGIII soybean varieties under different sowing dates, it was found that the varieties with more dominant E alleles showed longer VP, shorter RP and smaller R/V value. The effects of different E alleles on flowering time and maturity were different, and the effect of the dominant E alleles on spring- sowing soybeans was stronger than that on the summer-sowing ones. The agronomic traits for MGIII varieties from different regions were significantly different and that correlated with the growth period structure. The height of bottom pods of MGIII soybean varieties from Northern China was negatively correlated with R/V, while the number of pods per plant was positively correlated with R/V. Branch number, pod number per plant and 100-seed weight of YHH MGIII varieties were not correlated with R/V. The branch number of southern soybean varieties was significant negative correlated with the VP. These results provide a basis for the improvement of the growth period structure of soybean varieties and adaptability of soybeans to multiple environments. 相似文献
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Dan Zhao Hongwen Bi Zicheng Zhu Jianhui Liu Junmin Zhang 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2017,67(2):155-163
Cucurbita maxima is one of the most widely cultivated Cucurbitaceae in Heilongjiang province, China. The objectives of this study were to clarify the genetic diversity of 44 accessions from different geographical origins using morphological and molecular characteristics and to compare the consistency of these morphological and molecular characteristics. Morphological characteristics showed a large qualitative variability primarily according to fruit-related traits. Twenty-eight random-amplified polymorphic DNA (RAPD) markers produced 128 bands. Both morphological characteristics and RAPD markers grouped 44 accessions of C. maxima into four clusters, and the similarity coefficients ranged from 0.70 to 0.91 and from 0.40 to 0.98, respectively. DNA polymorphisms were highly consistent with phenotypic traits on rootstock C. maxima. The relationships between C. maxima from different origins were not clearly defined via morphological characteristics and RAPD analysis, suggesting some traits of C. maxima were specific to geographical location had disappeared or were weak in Heilongjiang province, which would not facilitate pumpkin breeding. Hybrid generations and their parent plants or sister lines were grouped into sub-clusters and showed little genetic distance according to both evaluation methods. Overall, morphological characteristics and RAPD markers were consistent and revealed high genetic diversity between C. maxima landraces from different origins. 相似文献