磷化铝间歇熏蒸防治鱼粉螨类的研究

本研究介绍了饲料鱼粉螨类的有效防治方法．采用11．5g／m2的磷化铝片剂，分两次间歇熏蒸大量孳生螨类和昆虫的棉纱布袋和编织袋包装的鱼粉，达到了有效杀灭鱼粉中大量孳生的粉尘螨、跗蠊螨、齿蠊螨、河野脂螨、食根嗜木螨、马六甲肉食螨、转开肉食螨、特氏肉食螨等8种螨类和烟草甲、白腹皮蠹、拟白腹皮蠹、赤颈郭公虫、赤足郭公虫、黑菌虫的效果。     

Nuclear transfer in loach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion

To study nuclear transfer in the loach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 °C in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days. It was found that gastrula cells incubated at 4 °C had the same totipotence as blastula cells. The optimal UV dosage for inactivation of the oocyte chromatin was 180–240 mJ cm⁻². Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.     

Movement of Xanthomonas campestris pv. vitians in the stems of lettuce and seed contamination

Xanthomonas campestris pv. vitians , the causal agent of bacterial leaf spot of lettuce (BLS), can be seedborne, but the mechanism by which the bacteria contaminates and/or infects lettuce seed is not known. In this study, the capacity of X. campestris pv. vitians to enter and translocate within the vascular system of lettuce plants was examined. The stems of 8- to 11-week-old lettuce plants were stab-inoculated, and movement of X. campestris pv. vitians was monitored at various intervals. At 4, 8, 12 and 16 h post-inoculation (hpi), X. campestris pv. vitians was recovered from 2 to 10 cm above (depending on stem length) and 2 cm below the inoculation site. Xanthomonas campestris pv. vitians was also recovered from surface-disinfested stem sections of spray-inoculated plants. Together, these results are consistent with X. campestris pv. vitians invading and moving systemically within the vascular system of lettuce plants. To investigate the mechanism of seed contamination, lettuce plants at the vegetative stage of growth were spray-inoculated with X. campestris pv. vitians and allowed to develop BLS. Seed collected from these plants had a 2% incidence of X. campestris pv. vitians external colonization, but no bacteria were recovered from within the seed.     

不同声音对草鱼幼鱼负趋音性行为反应影响研究

声驱鱼技术作为辅助过鱼措施之一,承担着保证鱼类洄游顺利通过过鱼设施,继而保护鱼类资源和恢复河流连通性的重要作用。本研究采用交替播音的形式,以草鱼(Ctenopharyngodon idellus)幼鱼为研究对象进行负趋音性实验,旨在探究草鱼幼鱼面对不同声音的行为反应。实验水槽(3.6 m×1.1 m×1.0 m)布置于下牢溪周围水域,平均水深为0.5 m,平均流速为0.06 m/s。实验使用1种单频音(1 000 Hz)和5种复杂音(鱼游动声、引擎声、短吻鳄叫声、打桩声和游艇声),声压级(sound pressure level)为(117.69±2.77) dB re 1 μPa,对照组为未播放声音时草鱼的行为反应数据。结果显示,播放复杂音时,草鱼的反应次数、趋音速度、运动时间比均显著高于单频音和对照组(P<0.001),草鱼的初次反应时间、平均反应时间均显著低于单频音和对照组(P<0.001);复杂音中,受到游艇声刺激的草鱼反应次数和趋音速度最大,受到鱼游动声刺激的草鱼反应次数、趋音速度最小;复杂音中,受到游艇声刺激的草鱼初次反应时间最短,为(23.40±5.13) s;受到引擎声刺激的草鱼初次反应时间最长,为(146.00±7.82) s,显著低于其他复杂音(P<0.05);受到游艇声和打桩声刺激的草鱼平均反应时间最短,分别为(26.52±3.01) s和(28.76±4.07) s;受到鱼游动声刺激的草鱼平均反应时间最长,为(64.76±17.82) s;复杂音中,受到鱼游动声刺激的草鱼运动时间比最高,为(98.47±0.48)%;受到引擎声刺激的草鱼运动时间比最低,为(94.58±0.54)%;播放单频音时,草鱼的反应次数、初次反应时间、平均反应时间、运动时间比均与对照组无显著差异(P>0.05)。本研究表明,5种复杂音(鱼游动声、引擎声、短吻鳄叫声、打桩声和游艇声)对草鱼幼鱼具有驱赶效果。本研究在丰富鱼类负趋音性研究的同时,为实际工程中声驱鱼辅助过鱼设施的设计和优化提供了科学依据。     

嘉陵江下游鱼类资源现状调查

2003年5月至10月对嘉陵江下游的鱼类资源进行了调查,并对该水域鱼类资源变动的原因进行了探讨。计调查226船次,统计渔获物1153kg,测量鱼类标本19378尾。共记录鱼类92种,隶属于5目14科61属,其中有13种为该水域新记录。结果表明嘉陵江水域生态环境的变化导致鱼类物种组成、种群结构发生了变化;珍稀鱼类、洄游性或半洄游性鱼类的种群数量急剧减少甚至绝迹,如中华鲟、达氏鲟、白鲟、胭脂鱼、等。     

基于水声学方法的嘉陵江中游蓬安段鱼类资源研究

研究嘉陵江中游鱼类资源时空动态特征,了解变化过程及影响因素,提出嘉陵江鱼类资源保护的建议和措施,同时为长江上游及其支流的鱼类资源保护提供基础数据。2018年7月（夏）、10月（秋）以及2019年1月（冬）、4月（春）,运用Biosonics DT-X分裂波束科学回声探测仪对嘉陵江中游蓬安段金溪航电工程干流江段进行了水声学探测,并辅以常规渔获物调查。共采集到鱼类6科38种,其中蛇鮈、鲫、银飘鱼、黄尾鲴等中小型鱼类在数量上占优;鱼类密度分别为春季（0.8296±0.43）、夏季（0.8705±0.38）、秋季（0.5082±0.25）、冬季（0.3939±0.13）尾/m3;鱼体平均目标强度分别为春季（-63.15±5.03）、夏季（-52.85±14.45）、秋季（-46.42±15.85）、冬季（-44.77±15.28）dB;鱼体平均重量分别为春季0.03、夏季1.08、秋季11.19、冬季20.40 g/尾;鱼类资源量分别为春季0.4096、夏季0.2907、秋季0.3919、冬季0.5622 kg/m3;坝下鱼类密度显著高于坝上（F=12.67,P<0.05）;坝上（F=7...     

Therapeutic Intervention with Dietary Chitosan Nanoparticles Alleviates Fish Pathological and Molecular Systemic Inflammatory Responses against Infections

Marine bio-sourced chitosan nanoparticles (CSNP) are antimicrobial and immunomodulatory agents beneficial for fish medicine. Herein, dietary CSNP was investigated for the amelioration of the systemic inflammatory responses of an induced fish model. One hundred and forty-four rainbow trout were assigned to one pathogen-free and non-supplemented group (negative control), and three challenged groups: non-supplemented (positive control), CSNP-preventive, and CSNP-therapeutic. After a feeding experiment extended for 21 days, the organosomatic indices (OSI) and molecular aspects were assessed. After a challenge experiment extended for further 28 days, CSNP-therapeutic intervention was assessed on fish survival and systemic inflammatory responses on pathology, histo-morphology, and molecular aspects. With CSNP administration, OSI nonsignificantly decreased and the relative expression of targeted inflammatory-mediator genes was significantly increased. The CSNP-therapeutic fish showed an RPS of 80% as compared to the positive control group, and CSNP-therapeutic administration retained the highest gene expression augmentation up to 28 days after the challenge. Notably, the splenic reticulin fibers framework of the CSNP-therapeutic group retained the highest integrity among the groups during the infection. After recovery, reticulin fibers density in the CSNP-therapeutic samples was significantly higher than in the negative control group, which indicates high innate immunity. Thus, CSNP showed promising biotherapeutic features enhancing fish resistance against infections.     

Histological, digestive, metabolic, hormonal and some immune factor responses in Atlantic salmon, Salmo salar L., fed genetically modified soybeans

The paper reports the second and final part of an experiment aiming to study physiological and health-related effects of genetically modified (GM) soybean meal (SBM) type Roundup Ready soybean (RRS) in diets for post-smolt Atlantic salmon. For 3 months salmon were fed diets containing 172 g kg(-1) full-fat SBM from RRS (GM-soy) or an unmodified, non-isogenic line (nGM-soy), or a reference diet with fishmeal as the sole protein source (FM). Slight differences in anti-nutrient levels were observed between the GM and nGM-soy. Histological changes were observed only in the distal intestine of the soy-fed fish. The incidence of moderate inflammation was higher in the GM-soy group (9 of 10 sampled fish) compared with the nGM-soy group (7 of 10). However, no differences in the concomitant decreases in activities of digestive enzymes located in the brush border (leucine aminopeptidase and maltase) and apical cytoplasm (acid phosphatase) of enterocytes or in the number of major histocompatibility complex class II+ cells, lysozyme activity, or total IgM of the distal intestine were observed. GM compared with nGM-soy fed fish had higher head kidney lysozyme (11,856 vs. 10,456 units g(-1) tissue) and a tendency towards higher acid phosphatase (0.45 vs. 0.39 micromol h(-1) kg(-1) body mass in whole tissue) activities, respectively. Plasma insulin and thyroxin levels, and hepatic fructose-1,6-bisphosphatase and ethoxyresorufin-O-deethylase activities were not significantly affected. It is not possible, however, to conclude whether the differences in responses to GM-soy were due to the genetic modification or to differences in soy cultivars in the soy-containing diets. Results from studies using non-modified, parental line soybeans as the control group are necessary to evaluate whether genetic modification of soybeans in diets poses any risk to farmed Atlantic salmon.     

Priority habitats for the conservation of large river fish in the Ganges river basin

• 1. Three classes of habitat used by groups of fish species classified as conservation and management priorities were developed for the Gerua River (also known as the Girwa River, Karnali River) in the Ganges river basin. This river is large (mean annual discharge ca 1500 m³ s^?1, up to 900 m wide), surrounded by protected lands of India and Nepal, and upstream of major diversions and river alterations.
• 2. Fish and habitat sampling was conducted at 45 sites from 2000 to 2003. Data were analysed for 2172 fish of 14 species. Species and life stages found occupying a statistically distinct subset of the river habitats were grouped to identify classes of river habitat for conservation.
• 3. Most species and life‐stage groups specialized on specific habitat conditions revealed by multivariate analyses of variance and a principal component analysis. The most numerous and diverse group (six species, 15 life stages) was associated with deep depositional habitats with sandy substrate. Two species covering three life stages were primarily oriented to erosional habitat marked by fast current velocity with pebble and cobble substrate. A third group of three species of adults and juveniles were intermediate in habitat use.
• 4. River conservation for fish faunas should maintain both erosional and depositional channel habitats with depths, substrates, and current velocity inclusive of the ranges reported. The erosional and depositional nature of the key habitats requires that rivers be maintained with flows capable of channel‐forming functions.

Copyright © 2006 John Wiley & Sons, Ltd.