首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  完全免费   3篇
  水产渔业   3篇
  2018年   1篇
  2017年   1篇
  2016年   1篇
排序方式: 共有3条查询结果,搜索用时 15 毫秒
1
1.
  1. Biological invasions are a global threat to biodiversity, and many arise from deliberate introductions.
  2. The American freshwater fish Micropterus salmoides and Ameiurus spp. (Ameiurus melas and Ameiurus nebulosus) were introduced to Europe for recreational fishing, Gambusia holbrooki and Gambusia affinis were introduced for mosquito population control, and Lepomis gibbosus was introduced as an ornamental species. The Asiatic Pseudorasbora parva was acquired inadvertently as an accompanying species in fish consignments.
  3. This article presents a novel approach for detecting these species directly from water samples based on a panel of five taxon‐specific primers within 16S rDNA.
  4. The primers were validated from tissue, in aquarium experiments, and from Ebro River water samples (Spain). With a simple polymerase chain reaction (PCR) protocol, followed by visualization in agarose gel or capillary electrophoresis, it was possible to detect these species from environmental DNA concentrations as low as 0.89–100 pg mL–1.
  5. This sensitive and economical tool can be used to control European invasions of these species and to preserve native biodiversity.
  相似文献
2.
  1. This study presents a species‐specific DNA‐based marker for detection of the zebra mussel Dreissena polymorpha, recognized as one of the worst invasive species worldwide.
  2. The marker was developed in silico and experimentally tested on environmental samples. Gel and capillary electrophoreses for visualization of the PCR products were compared.
  3. Marker specificity and sensitivity were assessed in vitro by cross‐amplifications and serial dilutions, respectively. The method allows detecting at least 0.7 ng of Dreissena DNA per μL and cross‐species amplification was not found in any case.
  4. Next‐generation sequencing (NGS) metabarcoding (PCR amplification and massive sequencing of a DNA barcode) was used as an independent method for verifying presence of Dreissena DNA molecules in environmental plankton samples collected from the south‐eastern Baltic Sea.
  5. The consistency between NGS results reporting presence of Dreissena and positive PCR amplification of the marker from the plankton samples confirmed the efficacy of this highly reproducible, fast, cheap and technically easy method.
Copyright © 2016 John Wiley & Sons, Ltd.  相似文献
3.
  1. Knowledge of species distribution is of utmost importance for conservation and management of endangered freshwater mussels. Conventional monitoring approaches are often time consuming and costly. The use of environmental DNA (eDNA) is a relatively new approach and considered as an effective tool to detect the presence of target species in aquatic environments. The aim of this study was to establish a nested PCR system to detect eDNA of Margaritifera margaritifera and to discuss the advantages and disadvantages of eDNA in mussel surveys compared with classical monitoring.
  2. DNA of M. margaritifera was detected in 2 L water samples collected directly downstream (25 m) from pearl mussel populations (population size from 800–20 000), with an internal‐nested PCR approach greatly increasing the detection sensitivity (down to 10 fg target DNA). eDNA detection at greater distances downstream (500 and 1 000 m) of these populations failed, possibly due to DNA degradation or dilution processes. eDNA was also detected downstream of an extinct population, most likely resulting from overlooked mussels or the release of DNA from dead shells.
  3. The eDNA approach proposed herein may be helpful in initial screening of streams that are otherwise difficult to monitor, or in the detection of buried juvenile mussels without disturbing their habitat. However, it cannot replace monitoring of population demography, and of other important information for conservation, and should thus only be seen as a supplementary tool.
Copyright © 2015 John Wiley & Sons, Ltd.  相似文献
1
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号