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  1. Biological invasions are a global threat to biodiversity, and many arise from deliberate introductions.
  2. The American freshwater fish Micropterus salmoides and Ameiurus spp. (Ameiurus melas and Ameiurus nebulosus) were introduced to Europe for recreational fishing, Gambusia holbrooki and Gambusia affinis were introduced for mosquito population control, and Lepomis gibbosus was introduced as an ornamental species. The Asiatic Pseudorasbora parva was acquired inadvertently as an accompanying species in fish consignments.
  3. This article presents a novel approach for detecting these species directly from water samples based on a panel of five taxon‐specific primers within 16S rDNA.
  4. The primers were validated from tissue, in aquarium experiments, and from Ebro River water samples (Spain). With a simple polymerase chain reaction (PCR) protocol, followed by visualization in agarose gel or capillary electrophoresis, it was possible to detect these species from environmental DNA concentrations as low as 0.89–100 pg mL–1.
  5. This sensitive and economical tool can be used to control European invasions of these species and to preserve native biodiversity.
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2.
  1. This study presents a species‐specific DNA‐based marker for detection of the zebra mussel Dreissena polymorpha, recognized as one of the worst invasive species worldwide.
  2. The marker was developed in silico and experimentally tested on environmental samples. Gel and capillary electrophoreses for visualization of the PCR products were compared.
  3. Marker specificity and sensitivity were assessed in vitro by cross‐amplifications and serial dilutions, respectively. The method allows detecting at least 0.7 ng of Dreissena DNA per μL and cross‐species amplification was not found in any case.
  4. Next‐generation sequencing (NGS) metabarcoding (PCR amplification and massive sequencing of a DNA barcode) was used as an independent method for verifying presence of Dreissena DNA molecules in environmental plankton samples collected from the south‐eastern Baltic Sea.
  5. The consistency between NGS results reporting presence of Dreissena and positive PCR amplification of the marker from the plankton samples confirmed the efficacy of this highly reproducible, fast, cheap and technically easy method.
Copyright © 2016 John Wiley & Sons, Ltd.  相似文献
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