不同饲养水平对瑶山鸡生产性能和胴体品质的影响

文章旨在研究不同饲养水平对瑶山鸡生产性能和胴体特征的影响。试验将400只平均体重一致的1日龄瑶山鸡随机分为2组,每组4个重复,每个重复50只。2组分别饲喂代谢能水平为3155 Kcal/kg、粗蛋白质水平为225 g/kg的高能高蛋白日粮和代谢能水平为2965 Kcal/kg、粗蛋白质水平为195 g/kg的低能低蛋白日粮。试验共开展10 w。同时将240只产蛋高峰期瑶山鸡随机分为2组,每组4个重复,每个重复30只,饲喂上述日粮4 w,试验结束后收集受精蛋进行孵化。结果:高能高蛋白组种鸡在8和10 w的体重和采食量均显著高于低能低蛋白组(P<0.05),而料重比显著降低(P<0.05)。高能高蛋白组种鸡受精率和孵化率显著高于低能低蛋白组(P<0.05),而残次率、胚胎死亡率和出壳前死亡率显著降低(P<0.05)。1~10 w时高能高蛋白组种鸡体重均显著高于低能低蛋白组(P<0.05),与低能低蛋白组相比,高能高蛋白组屠体重、可食用部分显著升高(P<0.05),腿肌占比显著降低(P<0.05)。结论:中等生长速度的瑶山肉种鸡可以更有效地利用高能量高蛋白(代谢能:3155 Kcal/kg,粗蛋白质:225 g/kg)饲料。     

采食后奶牛瘤胃短链脂肪酸及其吸收相关蛋白表达的研究

本试验旨在研究奶牛采食前后瘤胃中短链脂肪酸(SCFA)浓度的变化及其吸收相关蛋白表达量的差异。试验选用3头体重(720±30)kg且装有瘘管的健康荷斯坦牛(动物伦理审查编号为SXAU-EAW-2019-C002013),采食精粗比为40:60的日粮(10kg),试验预试期10d,于第11天饲喂前开始取样,采用气相色谱法检测奶牛采食前(0 h)和采食后(1、2、3、4、5、6、7、8 h)瘤胃液中SCFA浓度;并采用荧光定量PCR方法检测瘤胃上皮组织中与SCFA吸收相关的蛋白表达量。结果表明:在采食后1 h奶牛瘤胃中SCFA浓度最高(P<0.05);在采食后一段时间内(2~5h)与SCFA吸收相关蛋白表达量上调(P<0.05),AE2、MCT1基因表达量均在5 h最高,PAT1、NHE3基因表达量均在4 h最高,MCT4基因表达量在4、5、6h均较高,NHE1基因表达量在2h达到最高;AE2、MCT1、MCT4、NHE1基因表达量与SCFA浓度负相关或正相关(P<0.05),AE2、MCT1、MCT4基因表达量与瘤胃内pH正相关(P<0.05)。以上结果初步揭示,在采食后一定时间内,瘤胃中与SCFA吸收相关蛋白表达受SCFA浓度和pH的调节。     

球孢白僵菌-玉米共生体对亚洲玉米螟取食行为的影响

为明确球孢白僵菌-玉米共生体对亚洲玉米螟幼虫取食行为及取食选择性的影响，本文通过室内控制试验，采用培养皿叶盘法，研究了共生体对亚洲玉米螟初孵幼虫和3龄幼虫的选择、非选择性取食的影响。结果表明，在48 h非选择性试验中，亚洲玉米螟初孵幼虫在球孢白僵菌-玉米共生体叶片组织（处理组）上的取食率显著低于未接种球孢白僵菌玉米叶片（对照组），第48 h时在共生体和对照玉米上的幼虫取食率分别为35.7%和62.5%，差异极显著；3龄幼虫在共生体茎秆组织上的取食率与对照组玉米差异不显著。在48 h选择性试验中，初孵幼虫在共生体玉米叶片组织上的取食率显著低于对照组，共生体玉米叶片组织上的幼虫取食率随时间的增加呈下降趋势，第48 h时在共生体和对照玉米上的幼虫取食率分别为6.3%和20.6%，差异极显著；3龄幼虫在共生体和对照组玉米茎秆组织上的幼虫取食率随时间的增加呈上升趋势，其中对照组幼虫取食率显著高于处理组，第48 h时在处理组和对照组玉米上的幼虫取食率分别为33.1%和45.6%，差异极显著。本研究初步表明，球孢白僵菌-玉米共生体对亚洲玉米螟幼虫的取食行为有显著的抑制和忌避作用。     

饲养方式对产蛋前母鸡肉质、肌肉物理特征和感官评价的影响

文章旨在研究不同饲养方式对产蛋前蛋鸡胸肌和腿肌化学成分、物理特征和感官评价的影响。试验选择1日龄海兰褐壳蛋鸡900只,随机分为3组,每组5个重复,每个重复60只。蛋鸡在1~10周龄和11~20周龄时分别饲喂相同的生长前期和生长后期日粮,3个组蛋鸡分别通过笼养、平养(稻谷粉作为垫料)和散养。试验结束后,对蛋鸡屠宰,收集胸肌和腿肌进行分析。结果:平养和笼养组蛋鸡胸肌粗脂肪含量较散养组分别显著提高114.81%和74.07%(P<0.05),但散养组蛋鸡胸肌铁含量较平养组显著提高37.99%(P<0.05),同时胶原含量较笼养组显著提高25.28%(P<0.05)。散养组蛋鸡腿肌粗脂肪和能量含量最低(P<0.05),但散养组粗蛋白质、铁、锌含量分别较笼养组显著提高8.85%、40.26%和17.16%(P<0.05)。散养组蛋鸡胸肌硬度和韧性较笼养组分别显著提高42.69%和27.76%(P<0.05)。笼养组腿肌蒸煮损失较平养组显著提高8.01%(P<0.05),散养组腿肌硬度和韧性分别较平养组显著提高35.24%和133.25%(P<0.05)。与笼养组相比,散养组胸肌风味和咀嚼性显著高于笼养组(P<0.05),同时散养组胸肌总可接受度显著高于平养组(P<0.05)。散养组腿肌风味、多汁性和咀嚼性均显著高于笼养和平养组(P<0.05),同时该组总可接受度较平养组显著提高6.72%(P<0.05)。结论:散养产蛋前蛋鸡的感官指标表现最佳,同时腿肌和胸肌粗脂肪含量最低,粗蛋白质和胶原蛋白含量高,可以改善肌肉口感。     

鸡皮刺螨实验室饲养与繁殖技术研究进展

鸡皮刺螨是一种吸血性外寄生虫,会引起鸡消瘦、贫血,并导致产蛋下降,造成巨大经济损失。为给科研提供可靠虫源,并对杀螨药物、疫苗以及其他防控手段进行效果评价,国内外学者进行了鸡皮刺螨实验室的人工饲养与繁殖技术研究。文章综述了国内外鸡皮刺螨实验室饲养与繁殖技术的研究进展,将其总结归纳为使用动物饲养繁殖技术与离体饲养繁殖技术两类,同时指出了各项技术在应用过程中存在的问题,为该技术的进一步完善与发展提供相关参考。     

Effects of natural feeding stimulants and glutamic acid supplementation on the feed intake,growth performance and digestive enzyme activities of red sea bream (Pagrus major) fed fish meal‐free soy protein concentrate (SPC)‐based diet

Two experiments were conducted for red sea bream (Pagrus major). In experiment 1, the optimum level of glutamic acid and natural feeding stimulants to enhance feed intake were determined and found that glutamic acid level of 0.5% and fish meat hydrolysate (FMH) were effective. In experiment 2, fish were fed with soy protein concentrate (SPC)‐based diet with synthetic feeding stimulants (Basal diet), the Basal diet with FMH (FMH diet), the FMH diet with glutamic acid (FMHG diet) and with fish meal diet (FM diet) as a control until satiation for 8 weeks. Feed intake of FMHG‐fed fish was significantly higher than others (p < 0.05). Specific growth rate and the feed conversion ratio of FMHG were comparable to those of FM‐fed fish (p > 0.05). Relative visceral fat ratio and crude lipid content of any SPC‐based diet‐fed fish tended to be lower than those of FM diet‐fed fish. There were no significant differences in trypsin and lipase activities hepatopancreas among treatments. SPC can be utilized as a sole protein source in a diet for red sea bream. The lower growth performance in SPC‐based diet‐ fed fish was not due to poor digestive enzyme secretion but could be associated with lipid utilization disorder.     

Growth,survival rate and fatty acid composition of sterlet (Acipenser ruthenus) larvae fed fatty acid‐enriched Artemia nauplii

We evaluated the growth and survival rate of sterlet (Acipenser ruthenus) larvae fed Artemia nauplii enriched with Olioω3 or Red Pepper commercial emulsions (BernAqua NV, Belgium). Sterlet larvae, 0.022 ± 0.002 g body weight, were randomly assigned to one of three feeding regimes with two different feeding durations. After administering live feed for 7 or 14 days, larvae were weaned onto commercial food and reared to 36 days posthatching (28 days of feeding). There were no significant differences in body weight among groups at the end of the trial. A significantly higher survival rate (p < 0.05) was observed in larvae fed Artemia enriched with Red Pepper for 14 days compared to other feeding regimes. Based on the analysis of growth parameters, we can conclude that 7 days of live feeding to be sufficient for efficient rearing of sterlet larvae. And longer duration of live feeding with use of special enrichment can be recommended for a higher survival rate.     

Impact of Feeding Status on the Efficacy of Depuration for Decontaminating Vibrio parahaemolyticus in Pacific Oysters (Crassostrea gigas)

Vibrio parahaemolyticus causes acute human gastroenteritis and is often linked to consumption of raw oysters. Previous investigations indicated that refrigerated seawater depuration at 12.5°C could significantly reduce V. parahaemolyticus contamination in Pacific oysters; however, further optimization is necessary to achieve the regulatory target of >3.52 log most probable number (MPN)/g reduction. The current study investigated influences of algal feeding on efficacy of depuration to reduce V. parahaemolyticus in raw oysters. A V. parahaemolyticus cocktail (10290, 10292, 10293, BE 98–2029, 027-1c1) was mixed in artificial seawater (70 L) to inoculate oysters (n = 35) at 4–5 log MPN/g. Inoculated oysters were subjected to depuration with feed (algae = 0.036 ml/gram of oyster) and without feed at 12.5°C. Oysters (n = 5) were analyzed for V. parahaemolyticus using a three-tube MPN method after 0, 1, 3, 5, and 6 days of depuration. Depuration (6 days) achieved average V. parahaemolyticus reductions of 2.75 log MPN/g and 3.03 log MPN/g in the fed and unfed systems, respectively; however, feeding status did not significantly impact the efficacy of depuration to reduce V. parahaemolyticus in Pacific oysters. Further optimization of depuration is necessary to achieve the regulatory target for V. parahaemolyticus decontamination in raw oysters.     

明轮驱动虾塘自主导航投饵船设计与可靠性试验

基于明轮驱动的虾塘投饵船能够适应养殖池塘复杂的环境、满足全塘抛撒的要求，可靠性是其进行推广的关键。采用滚塑工艺设计了全封闭投饵船体，利用免油脂润滑不锈钢链轮和明轮作为驱动机构，以避免对水体的污染，螺旋输送饵料装置可满足船载投饵过程中重心位置稳定的要求，通过GPS+电子罗盘的方式实现了自主导航定位和姿态控制需求。根据虾塘投饵和控制性能要求，进行投饵船直线运动和转弯运动模型的构建，采用PID航向、航速运动控制算法进行巡航路径控制，池塘测试平均速度为0.72m/s，直行和转弯最大偏航量分别为0.8m和0.5m。40d的养殖塘现场试验结果表明，自主导航投饵船在复杂路径下运行平稳，可满足虾塘饵料投喂要求，同时对强风、大雨等恶劣环境进行了可靠性测试，发现并解决了相关问题。