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草鱼出血病细胞培养弱毒疫苗的制备及其免疫效果   总被引:1,自引:1,他引:10  
本文报道经筛选的GCHV-841毒株在草鱼吻端成纤维细胞系中进行适应性培养,弱毒驯化,细胞培养弱毒疫苗的制备,安全性观察,不同稀释度和剂量的免疫保护率测定及保存试验,GCHV-841毒株在PSF细胞中继代驯化至53代达到减毒目的。筛选得53-59代作毒株在PSF细胞中继代驯化至53代达到减毒目的,筛选得53-59代作毒种制备的细胞制备细胞弱毒疫使用安全,用稀释10-10^4倍的疫苗,0.1-0.3  相似文献
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张雪娟 《水产学报》1994,18(3):190-190
评《水产动物疾病学》《水产动物疾病学》一书已于1993年11月由上海科学技术出版社出版了。它是我国出版的第一本内容包括淡水、海水及海淡水增养殖鱼类、甲壳类、软体类、两栖类和爬行类等水产经济动物疾病的病原、流行情况、症状、病理、诊断、预防和治疗知识的专...  相似文献
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A genetically attenuated strain of Aeromonas salmonicida has been developed that has a complete deletion of the aroA gene (Brivax II), making it suitable for development as a commercial vaccine. Brivax II was effectively cleared from Atlantic salmon, Salmo salar, a species highly susceptible to furunculosis, confirming that it is attenuated. Clearance rate was dependent on the vaccine dose administered, being longer with higher doses. Immunological studies using Brivax II injected in rainbow trout, Oncorhynchus mykiss, confirmed that live vaccines stimulate a greater response, in terms of generating leucocytes able to proliferate to a subsequent encounter with antigen, relative to killed vaccines. Development of strains of Brivax II as carriers of heterologous antigens was also investigated. Escherichia coli -galactosidase was chosen as the model antigen, and three strains containing plasmids with the LacZ gene were constructed (Brivax 12, Brivax 61 and Brivax 107). All three strains were shown to express -galactosidase in vivo in rainbow trout and to be cleared effectively. Interestingly, Brivax 107 was cleared faster than the other two Lac+ strains and had the highest level of -galactosidase activity. The two strains expressing lower levels of activity also behaved differently in vivo, in that Brivax 12 accumulated derivatives expressing lower levels of -galactosidase activity, suggesting that mutants are being selected in vivo. The potential advantages of live vaccines over killed vaccines are discussed.  相似文献
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