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为探究“金花散茶”及其“金花菌粉”对被动吸烟(Cigarette smoking environment,CSE)小鼠肺组织受损的预防及修复机制,建立C57BL/6小鼠CSE模型,以600 mg∙kg-1剂量的金花散茶茶汤(Eurotium cristatum tea extract,ECTE)及金花菌粉浸提液(Eurotium cristatum powder extract,ECPE)进行灌喂处理。与CSE模型组相比,小鼠灌喂ECPE和ECTE后,肺组织病理学切片显示其可保护小鼠肺组织形态结构完整;酶联免疫分析显示,灌喂ECPE和ECTE可显著抑制小鼠血清IL-6、IL-8、IL-1β、IFN-γ和TNF-α表达量上调;Western blot结果表明,灌喂ECPE和ECTE对小鼠肺组织p-JAK2、p-STAT3、p-JAK2/JAK2、p-STAT3/STAT3高表达起到抑制作用。以上研究结果表明,灌喂ECPE、ECTE对CSE肺受损小鼠具有明显保护作用,总体趋势为ECPE组优于ECTE组、预防组优于治疗组。  相似文献   
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为探究回乳期奶牛血清中促乳素(PRL)、雌二醇(E2)、孕酮(P4)、信号转导和转录激活因子5(STAT5)含量和产奶量变化规律及其相关性,选择规模化奶牛场产奶量为(15.43±0.60)kg,将干乳的妊娠后期奶牛20头为研究对象。干乳开始当天记为第0天,分别在第0、1、3、5、7、9和11天上午8:00采集奶牛尾静脉血,ELISA法检测血清中PRL、E2、P4和STAT5含量并对结果进行统计学处理,分析其在奶牛回乳过程中的变化规律及其相关性。结果表明:回乳期奶牛血清PRL含量和产奶量在0~1d变化不显著(P0.05),3~11d依次降低且差异极显著(P0.01);血清E2含量0~1d、3~5、7~9d、9~11d之间差异均不显著(P0.05),依次呈阶梯式下降趋势;血清STAT5含量同样呈下降趋势,回乳0~5d、7~11d差异不显著(P0.05),但0~5d与7~11d两阶段间差异极显著(P0.01);血清P4含量从回乳0~11d变化不显著(P0.05);回乳期奶牛产奶量与PRL、E2、STAT5含量呈极显著正相关(P0.01),PRL、E2和STAT5含量变化两两间均呈极显著正相关(P0.01);P4含量变化与PRL、E2、STAT5含量和产奶量变化的相关性均不显著(P0.05)。综上所述,在奶牛回乳期间,血清中PRL、E2和STAT5含量均呈降低趋势并显著正相关,而P4含量变化不显著且与其他激素无显著相关性,为进一步研究泌乳相关激素调控奶牛回乳的作用机理提供理论依据。  相似文献   
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为探索EGCG对Nicotine诱导肺癌细胞增殖的抑制作用,本研究通过MTT实验筛选出EGCG、Nicotine对肺腺癌细胞H1299的最佳作用浓度,利用实时荧光定量PCR技术检测EGCG、Nicotine对H1299细胞中Bax、Bcl-2、Jak2和Stat3基因mRNA相对表达量的变化。实验结果表明,EGCG对H1299细胞的半抑制浓度IC50值约为32μmol·L-1(24 h)、15μmol·L-1(48 h);1μmol·L-1的Nicotine对H1299细胞促增殖作用明显;以15μmol·L-1的EGCG预处理H1299细胞24 h可显著下调1μmol·L-1 Nicotine的促增殖作用(P0.05)。1μmol·L-1的Nicotine处理H1299细胞可明显降低JAK2/STAT3信号通路中Bax基因mRNA的表达,增加Bcl-2、Jak2、Stat3基因的mRNA表达;15μmol·L-1 EGCG预处理H1299细胞可反向调控Nicotine诱导所致JAK2/STAT3信号通路中Jak2、Stat3和Bax、Bcl-2基因表达量的变化,结果具有显著性差异(P0.05)。由此可知,EGCG对Nicotine诱导的肺腺癌H1299细胞增殖及JAK2/STAT3信号通路中促增殖基因mRNA的表达起抑制作用。  相似文献   
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AIM: To investigate the effects of 17-AAG on apoptosis and cell cycle of HCT-15 cells and to clarify the related mechanisms. METHODS: MTT method was employed to evaluate the inhibitory effects of 17-AAG with Aifferent time and different doses on the proliferation of HCT-15 cells. The cells were stained with Annexin V-FITC/propidiumiodide and measured by flow cytometry. The expression of STAT3, cyclin D1, Cyt C, caspase 9 and caspase 3 at mRNA and protein levels was determined by RT-PCR and Western blotting. RESULTS: Treatment with 17-AAG at concentration of 1.25~20 mg/L for 24 h and 48 h significantly inhibited the activity of HCT-15 cells at both time-and concentration-dependent manners. Treatment with 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h significantly induced apoptosis and cell cycle arrest of HCT-15 cells. The exposure of 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h to the HCT-15 cells significantly down-regulated the expression of STAT3 and cyclin D1 at mRNA and protein levels, but up-regulated Cyt C, caspase 9 and caspase 3 mRNA and protein in a concentration-dependent manner. CONCLUSION: 17-AAG inhibits the cell activity, induces apoptosis and G1 arrest by down-regulating the expression of cyclin D1, and promoting the mitochondria apoptosis through STAT3 pathway.  相似文献   
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本研究通过探讨STAT5A基因SNP与山羊生长性状关联性,旨在为山羊选种选育提供更好的科学依据。实验以贵州白山羊和贵州黑山羊为研究对象,采用DNA池法及PCR-SSCP技术检测STAT5A基因单核苷酸多态性。结果发现,在贵州白山羊和贵州黑山羊STAT5A基因内含子10均检测到1个SNP位点G127A,表现为3种基因型,分别命名为GG、GA和AA。基因型与生长性状关联分析显示,贵州黑山羊基因型GA个体的胸围和体重指标显著高于基因型GG个体(p〈0.05),而其余3个指标均差异不显著(p〉0.05);贵州白山羊基因型GA个体的体斜长、胸围和管围指标显著高于基因型GG个体(p〈0.05),而其余指标均差异不显著(p〉0.05)。研究结果提示:STAT5A基因可能是影响山羊体重、体斜长、管围和胸围的主效基因或与主效基因连锁,G127A位点可望作为提高山羊个体生长性状的分子遗传标记。  相似文献   
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探讨连翘酯苷对人工感染传染性支气管炎病毒雏鸡的肺组织JAK/STAT信号通路的调节作用,分别设连翘酯苷预防组高、中、低和治疗组高、中、低6个剂量组,并设利巴韦林阳性对照组、空白对照组和病毒对照组。提取雏鸡肺组织总RNA,用相对荧光定量RT-PCR的方法测定雏鸡体内干扰素-α,JAK,STAT-1mRNA的相对表达量。结果表明连翘酯苷预防组高剂量100 mg/kg、中剂量50 mg/kg和治疗组中剂量100 mg/kg对雏鸡体内干扰素-α的mRNA水平表达有诱生作用(P〈0.01),治疗组中剂量显著上调JAK及STAT-1的表达,与JAK/STAT信号通路一些因子的上调相关,其机制部分与肺组织的JAK/STAT信号通路激活和相关信号的表达有关,这是连翘酯苷发生抗病毒作用的机制之一。  相似文献   
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AIM: To observe the effects of Yangxue-Jiedu (YXJD) decoction on imiquimod-induced psoriasis-like lesions in STAT3 transgenic mice.METHODS: STAT3 transgenic mice (n=24) were randomly divided into 4 groups: control group (using purified water for oral administration), model group (topical 5% imiquimod 42 mg and using purified water for oral administration), YXJD groups (topical 5% imiquimod 42 mg and using YXJD decoction for oral administration), and methotrexate (MTX) group (1 mg/kg MTX solution for oral administration, with the same topical imiquimod as model group). On day 7, the skin lesions were collected for examination. The lesions were evaluated according to the psoriasis area and severity index (PASI). The skin barrier function was evaluated by assessing oil and water components in the skin. The inflammation of psoriasis-like lesions was assessed by histological method. The expression of proliferating cell nuclear antigen (PCNA) and CD3 was assessed by immunohistochemical staining. The mRNA expression of IL-17A, IL-17C, IL-22 and RORγt was detected by real-time PCR. The levels of JAK/STAT3 pathway-related proteins in isolated T cells were determined by Western blot.RESULTS: Administration of YXJD decoction inhibited imiquimod-induced keratinocyte proliferation and infiltration of CD3+ T cells in psoriatic lesions, and ameliorated the epidermal barrier by up-regulation of the oil and water components in psoriatic lesions. Meanwhile, administration of YXJD decoction improved the systemic immune responses by reducing the weight of the spleen. The inflammatory cytokines IL-17A, IL-17C, IL-22 and RoRγt, and the levels of JAK/STAT3 pathway-related proteins STAT3, p-STAT3, JAK3 and p-JAK3 were decreased by administration of YXJD decoction.CONCLUSION: YXJD decoction likely alleviates imiquimod-induced psoriasis-like lesions in the STAT3 transgenic mice by inhibiting the phosphorylation of STAT3, and reducing the expression of IL-17A, IL-17C, IL-22 and RORγt.  相似文献   
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AIM: To investigate the effects of sodium valproate (VPA) on the proliferation of multiple myeloma cell lines RPMI8226 and U266 and the regulation of IL-6/JAK/STAT signaling pathway. METHODS: The cells were treated with different concentrations of VPA for 12 h and 24 h. The growth of RPMI8226 cells and U266 cells was detected by MTT assay. Apoptotic rates and cell cycle were analyzed by flow cytometry. The mRNA expression of STAT3, STAT5 and STAT target genes Bcl-xL, Mcl-1, c-Myc, CCND1 and VEGF was measured by RT-PCR. Western blotting analysis was used to determine the total proteins and protein phosphorylation levels of JAK2 and STAT5. RESULTS: VPA inhibited the growth and induced the apoptosis of RPMI8226 cells and U266 cells in a concentration- and time-dependent manner. The levels of IL-6 in the culture supernatants of RPMI8226 cells and U266 cells treated with VPA were significantly higher than that in negative control group. VPA down-regulated the mRNA expression of STAT3, STAT5, Bcl-xL, Mcl-1, c-Myc, CCND1 and VEGF. After treated with VPA, the protein levels of p-JAK2, JAK2, p-STAT5 and STAT5 in RPMI8226 cells and U266 cells were significantly lower than those in control group. CONCLUSION: VPA inhibits the proliferation of PRMI8226 cells and U266 cells in vitro. The modulation of IL-6/JAK/STAT signaling pathway may be involved in its potential mechanisms.  相似文献   
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