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1.
The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza⁃tion. Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health. Nowadays, the common detection methods are instrument-based for carbamate pesticide residue, which dis⁃ satisfy the request of an on-site rapid, sensitive and simultaneous detection for multiple carbamate pesticide resi⁃ dues. Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity, a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min. Under the optimized conditions, the limits of detection(LODs)were found to be 0.11 and 0.09 ng/g, while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively. By using spiked peanut samples, it recorded recoveries of 88.4%-98.3%, 90.1%-118.8% for carbofuran and carbaryl respectively. The RSD of intraarray were found at 9.0%, 3.5%, while the RSD of inter-array were recorded at 12.3%, 16.3% respectively. The de⁃ tection results of the microarray were highly consistent with that of HPLC-MS/MS. This proposed microarray provid⁃ ed an on-site high-sensitivity, simultaneous and rapid detection of carbofuran and carbaryl, and could be widely used in the fields of agricultural product quality safety, food safety and environmental monitoring.  相似文献   
2.
Two experiments were conducted to evaluate the effects of Fe injection timing after birth on suckling and subsequent nursery and growing-finishing pig performance. The injectable Fe source used in both experiments was GleptoForte (Ceva Animal Health, LLC., Lenexa, KS). GleptoForte contains gleptoferron which is a Fe macromolecule complex. In Exp. 1, a total of 324 newborn pigs (DNA 241 × 600, initially 1.6 ± 0.04 kg body weight [BW]) within 27 litters were used. Two days after birth, all piglets were weighed, and six barrows and six gilts per litter were allotted to 1 of 6 treatments consisting of no Fe injection or 200 mg of injectable Fe provided in a single injection on d 2, 4, 6, 8, or 10 of age. Pigs were weaned (~21 d of age) and allotted to nursery pens with all pigs in each pen having received the same Fe treatment. In Exp. 2, a total of 1,892 newborn pigs (PIC 359 × C40; initially 1.5 ± 0.02 kg BW) within 172 litters were used. One day after birth, piglets were weighed, and 11 pigs within each litter were allotted to 1 of 6 treatments consisting of no Fe injection or 200 mg of injectable Fe provided on d 1, 3, 5, or 7 of age, or 200 mg on d 1 plus 200 mg on d 12 of age. Pigs were weaned (19 d of age) and placed in a commercial wean-to-finish facility in a total of 15 pens with equal representation of treatments in each pen. In both experiments, not providing an Fe injection after birth decreased (P < 0.05) preweaning average daily gain (ADG), weaning weight, and hemoglobin and hematocrit values compared with all other treatments. In Exp. 1, increasing the age that piglets received an Fe injection until 4 or 6 d after birth provided marginal evidence for an improvement (quadratic; P = 0.070) in preweaning ADG. For the nursery period, increasing the age that piglets received an Fe injection improved (quadratic; P = 0.013) d 80 BW, but there was no evidence of a difference (P > 0.10) in d 173 BW at the end of the grow-finish period. In Exp. 2, increasing the age that piglets received a 200 mg Fe injection showed no evidence of difference (P > 0.10) for subsequent nursery and growing-finishing ADG. In both experiments, hemoglobin and hematocrit values were decreased (linear; P < 0.05) at weaning with increasing age when pigs received an Fe injection. These experiments suggest that providing a 200 mg Fe injection within 7 d after farrowing is sufficient for optimizing preweaning and subsequent growth performance.  相似文献   
3.
在丝绸之路经济带的大背景下,中国农产品的贸易发展速度加快,经济链条得到延伸。陕西省作为丝路经济带的起点,其农产品的发展却面临着来自国内外农产品的强力冲击,为了能够更好地满足经济全球化的市场需求,促进我国农业经济的发展,强化对于陕西省农产品的品牌建设。本文通过对陕西省农产品品牌现状的分析,围绕品牌观念、技术支撑、市场开发三个方面,基于政府和企业角度探讨陕西省农产品品牌建设的对策,并据此提出了加快陕西省特色农产品品牌发展的建议,以期对陕西省农产品品牌建设提供创新思路。  相似文献   
4.
以重庆市江津区为研究区,采集2019年生产的63份大米籽粒和64份玉米籽粒,用电感耦合等离子质谱仪(ICP–MS)和原子荧光光谱仪(AFS)测定大米和玉米籽粒中砷(As)、钡(Ba)、镉(Cd)、铜(Cu)、铁(Fe)、锰(Mn)、钼(Mo)、镍(Ni)和锌(Zn)的质量分数,并利用环境健康风险模型评价江津区大米和玉米中重金属的健康风险。结果表明:江津区大米和玉米籽粒中As最大质量分数分别为176.0、162.8 μg/kg,低于国家食品卫生As标准(<200 μg/kg);江津区3份大米和6份玉米样品中Cd质量分数超过国家食品卫生Cd标准(大米Cd质量分数<200 μg/kg、玉米Cd质量分数<100 μg/kg);大米籽粒中As和Mo质量分数均值显著高于玉米籽粒的(P<0.05),Cd质量分数均值略高于玉米籽粒的;大米籽粒中As、Cu、Mn和Zn的单因子污染指数均小于1,属于清洁水平,3份大米籽粒样品中Cd属于轻度污染;大米和玉米籽粒重金属综合污染指数分别为1.09和1.57,属于轻度污染,江津区玉米籽粒中重金属污染程度大于大米籽粒;大米籽粒中重金属As和Cd的致癌风险分别占健康总风险的82.7%和16.8%,表明江津区大米籽粒中As含量虽低于国家食品卫生标准安全值,但其健康风险较高,其次为Cd。可见,重庆地区需重点监控农作物中As和Cd的健康风险。  相似文献   
5.
为探明除草剂胁迫对3种牧草的化感作用,本研究采用室内生测法,采用不同推荐剂量的草铵膦和草甘膦分别处理紫花苜蓿(Medicago sativa)、黑麦草(Lolium perenne)和红豆草(Onobrychis viciaefolia)幼苗,测定3种牧草浸提液对受体小白菜(Brassica chinensis)种子萌发和幼苗生长的影响,并结合化感效应值对牧草的化感活性进行分析,旨在为牧草田的杂草防治及科学使用除草剂提供理论参考。结果表明:3种牧草浸提液对受体植物发芽率、发芽势、发芽指数、苗高、最大根长及活力指数均具有一定的影响,对最大根长的影响最大;受2种除草剂胁迫后,供试牧草的化感作用均显著高于清水处理,其中草甘膦胁迫后的表现更为明显,其对供试牧草化感作用的强弱顺序为:红豆草>紫花苜蓿>黑麦草。综上,除草剂胁迫下可明显增强紫花苜蓿、红豆草和黑麦草的化感作用。  相似文献   
6.
为探究圆柏(Sabina chinensis)的化感作用,本研究采用培养皿滤纸法研究圆柏叶片浸提液对地肤(Kochia scoparia)、稗草(Echinochloa crusgalli)、藜(Chenopodium album)、反枝苋(Amaranthus retroflexus)、马齿苋(Portulaca oleracea)5种杂草种子和向日葵(Helianthus annuus)、燕麦(Avena sativa)、油菜(Brassica campestris)、荞麦(Fagopyrum esculentum)4种作物萌发和生长的影响,并用圆柏叶片、树枝、树皮粉末和浸提液进行盆栽抑草效果检测。结果表明:圆柏叶片浸提液对5种杂草种子萌发和幼苗生长具有显著的抑制作用,对地肤、藜、反枝苋萌发及生长的化感效应指数均在-0.98以上;盆栽抑草结果表明,圆柏不同部位对5种杂草种子萌发和稗草及反枝苋株高生长均有较好的抑制作用,叶片化感强度大于树皮和树枝,叶片粉末拌土处理抑草效果好于浸提液叶面喷施;对作物的化感作用结果表明,圆柏叶片浸提液稀释1.5倍时,抑制荞麦种子萌发但不影响其株高及根生长,不影响燕麦种子萌发但降低其株高和根长生长,抑制油菜的发芽势和发芽指数但不影响其种子萌发率,促进油菜株高及根长生长,不影响向日葵种子萌发和生长。综上所述,圆柏具有良好的抑草活性,对作物影响较小。  相似文献   
7.
张伟强 《农学学报》2021,11(8):48-52
以福建特色水果‘油㮈’为试材,在等肥料成本条件下,进行水肥一体化施肥和常规施肥下‘油㮈’树枝梢生长长度和粗度、果实品质、产量表现及施肥成本的比较研究,以期为山地水肥一体化施肥提供参考。结果表明,水肥一体化施肥可在一定程度上促进当年枝梢长度和粗度的增长,提高果实可溶性糖、可溶性固形物及Vc含量,降低可滴定酸含量,提高果实平均单果重、可食率、产量。等价施肥条件下,水肥一体化施肥节省人工成本1104.0元/hm2。建议结合降水量等气候条件及山地土壤特点、生态环境等对水肥一体化施肥技术进行优化。  相似文献   
8.
为明确不同施氮水平下生物可降解膜覆盖对机插水稻生长和产量的影响,2019年和2020年在中国水稻研究所富阳试验基地开展大田试验,设置普通机插条件下0及13 kg/667 m2两个氮肥水平,生物可降解膜覆盖机插种植下13 kg/667 m2和 11 kg/667 m2 两个氮肥水平。结果表明,相同施氮量下,与不覆膜相比,覆膜机插水稻产量2019年和2020年分别增加6.1%和2.6%,成熟期干物质积累量分别增加17.4%和5.3%;在覆膜机插条件下,氮肥减施15%,与氮肥不减施处理相比水稻产量及干物质积累量差异不大,但氮肥偏生产力显著提高,说明覆膜机插在保障一定产量的条件下,能够减少氮肥施用,避免过量施肥造成环境污染,为水稻稳产和绿色高效提供一种新的种植方式。  相似文献   
9.
This study used two different collectors made of polypropylene (PP) rope and polyethylene (PE) fishing net to determine Mediterranean mussel's settlement, growth, condition index (CI), meat yield (MY) and shell thickness index (STI) during the period spent between June 2017 and November 2018. With this regard, chlorophyll a, water temperature, salinity, pH and particulate matter were defined through water samples that were collected on monthly basis triplicate. The mean chlorophyll a level was recorded as 0.32 ± 0.31 μg/L, while water temperature was 19.73 ± 5.14°C, salinity was 35 ± 2.070‰, pH was 8.12 ± 0.04, and TPM was 14.91 ± 10.48 mg/L. As a result of the cultivation period of 8 months spent following the first intense grip, the length of the mussels is measured as 31.79 ± 6.20 mm. Based on the Pearson correlation analysis results, the most important environmental parameter affecting Mediterranean mussels growth in length on PP rope collectors is the temperature (p < .05). The STI and CI were determined to be related with environmental temperature parameters, while no determinations could be obtained concerning the correlation of MY with environmental parameters. Assessment of Mediterranean mussels’ growth rate in PP rope collectors proved significant differences (p < .05) between March and November 2018. As no Mediterranean mussels are observed on PE fishing nets during the samplings performed throughout the study period, it is determined that PE fishing nets are not suitable collector types for Mediterranean mussel cultivation.  相似文献   
10.
以杂交稻组合隆两优华占及其2个亲本隆科638S和华占为试验材料,萌发期设置6个NaCl质量分数梯度(0.2%、0.4%、0.6%、0.8%、1.0%和1.2%)和苗期设置3个NaCl质量分数梯度(0.4%、0.8%和1.2%)来模拟盐胁迫试验,以清水为对照,分析盐胁迫对杂交稻及其亲本种子萌发和幼苗生长的影响。结果表明,在各质量分数的盐胁迫处理下,萌发期各项指标均不同程度地受到盐胁迫影响,多数指标值均随盐质量分数升高而下降,且质量分数0.6%是隆两优华占及其亲本的耐盐临界值。在0.2%~0.6%NaCl胁迫下3个品种发芽率、发芽指数、活力指数、胚芽长的盐胁迫抑制率在品种间存在显著差异,进一步通过隶属函数值综合评价,得出3个品种萌发期耐盐性由强到弱分别为隆两优华占、华占和隆科638S;苗期盐胁迫下3个品种中隆两优华占地上部分K^+/Na^+比值最大,表明其耐盐性最强。综合萌发期隶属函数值和苗期K^+/Na^+比值可以得出,杂交组合隆两优华占与其亲本比较,耐盐性更强,说明水稻的耐盐性具有一定的杂种优势。  相似文献   
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