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AIM:To investigate the effect of oridonin on the invasion and migration of human lung cancer NCI-H460 cells. METHODS:NCI-H460 cells were divided into high-dose (HD), middle-dose (MD) and low-dose (LD) oridonin groups (cultured with 40, 20 and 10 μmol/L of oridonin, respectively, as experimental groups), and normal (N) group (treated without oridonin as control). The cell growth was observed. The cell proliferation was detected by MTT assay. Boyden chamber was used to determine the cell invasive capacity. The cell migration was also measured. The levels of MMP-2 and MMP-9 were assayed by Western blotting. RESULTS:The cell counts in the experimental groups were lower than that in N group. The cell proliferation was inhibited as the inhibitory rates were 48.94%, 36.17% and 19.15% for HD group, MD group and LD group, respectively. The numbers of the invasive cells were 26.67±5.16 for HD group, 36.17±5.08 for MD group, and 44.33±5.50 for LD group. The migration rates in the experimental groups were lower than that in N group. The expression of MMP-2 and MMP-9 decreased dependent on the oridonin dose as follows: HD group < MD group < LD group < N group. CONCLUSION:Oridonin inhibits the invasion and migration of NCI-H460 lung cancer cells, and reduces the expression of MMP-2 and MMP-9.  相似文献   
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[目的]应用RP-HPLC法测定大叶香茶菜中冬凌草甲素的含量。[方法]RP-HPLC法色谱条件:色谱柱为Varian Microsorb-MV 100-5C18柱,流动相为甲醇∶水(1∶1),流速1.0 ml/min,检测波长λ=238 nm,柱温30℃。[结果]冬凌草甲素加样回收率为98.6%,RSD为2.04%(n=5);大叶香茶菜叶中冬凌草甲素的含量为4.79 g/kg。[结论]该法简便可靠,适用于大叶香茶菜中冬凌草甲素含量测定。  相似文献   
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AIM: To investigate the effects of oridonin on the invasion and migration of hepatocelluar carcinoma cells. METHODS: Human hepatocelluar carcinoma MHCC-97H cells were cultured and treated with 5, 10 or 20 μmol/L oridonin. The migration ability was measured by wound healing assay. The invasion ability was examined by Transwell invasion assay. The adhesion capabilities were evaluated by adhesion assay. The protein levels of LIM kinase-1 (LIMK-1), cofilin and phosphorylated cofilin (p-cofilin) were determined by Western blot. RESULTS: Oridonin inhibited the migration, invasion and adhesion abilities of MHCC-97H cells in a dose-dependent manner (P<0.05). After oridonin treatment, the expression of cofilin had no obvious change, but the protein levels of LIMK-1 and p-cofilin decreased significantly. CONCLUSION: Oridonin inhibits the invasion and migration of MHCC-97H cells. The mechanism may be related with the regulatory effect of oridonin on LIMK-1/cofilin signal transduction pathway.  相似文献   
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