东北稻蟹中18种微量元素含量及健康风险评价

为探讨东北地区（黑龙江省、吉林省和辽宁省）稻田养殖河蟹的微量元素含量水平和食用安全性，于2017年9月在东北主要养殖区采集稻田养殖河蟹56份，利用电感耦合等离子体质谱法（ICP-MS）检测了河蟹体内18种微量元素的含量，并采用单因子污染指数（P_i）和每周可耐受摄入量（PTWI）、目标危害商值（THQ）法分别评价了其污染程度和食用安全性。结果表明：河蟹中检出的大多数微量元素含量远高于该地区池塘养殖的淡水鱼类；吉林省河蟹体内的Cr、Cd、U等10种微量元素含量与其他两省份相比存在显著性差异（P<0.05）；所有样品的Cu、Pb、Cd和Cr含量均未超出国家食品安全标准；5种重金属（Cu、Zn、Pb、Cd和Cr）的成人每周实际摄入量（AWI）低于食品添加剂联合专家委员会（JECFA）推荐的每周可耐受摄入量；5种重金属和其他4种有害微量元素（Ba、U、Ag和Be）的人体每日摄入量（EDI）低于美国环保署（USEPA）推荐的参考剂量（RfD）。研究表明食用东北稻田养殖河蟹摄入的微量元素对食用人群没有明显的健康风险。在检测的18种微量元素中，Ba、Pb和Cd对复合污染物风险指数（HI）贡献率较高，是主要的风险元素。     

Biochemical and Microbiological Assessment of Crucifix Crab (Charybdis feriatus) Stored at 4°C

Charybdis feriatus is a highly prized crab in the local and international markets of East Asian countries. In local markets, crabs are sold live, iced, or refrigerated at 2°C – 4°C. The present study was carried out to determine the shelf life of whole crab stored at 4°C for 192 h using a set of biochemical, microbiological, and sensorial parameters. Biochemical indicators such as the total volatile base nitrogen (TVB-N) and trimethylamine nitrogen (TMA-N) increased significantly during storage and within an acceptable limit up to 144 h. Regarding the biogenic amines, cadaverine and spermidine increased significantly (p < 0.05) during the storage period. Among them, cadaverine could be proposed as a useful spoilage indicator for this crab species. The mesophilic and psychotropic bacterial count exceeded the limit of acceptance after 144 h of storage. Sensory attributes were gradually diminished with the storage time. Sensory scores were found to correlate well with both mesophiles and psychrotrophs. Based on the results of this study, the meat quality of Charybdis feriatus can be maintained up to 144 h when properly stored at a temperature of 4°C ± 1°C.     

Quality assessment of newly hatched mud crab,Scylla serrata,larvae

Starvation and exposure to formalin were investigated as possible stress tests for evaluating the quality of mud crab, Scylla serrata, larvae. For the starvation stress test, newly hatched zoeae stocked in 150‐ml containers were either starved or fed rotifers. Similarly, newly hatched zoeae were stocked in containers with seawater of 0 (control), 20, 30 and 40 mg/L formalin for the formalin stress test. The zoeae from the same batches were used for seed production to monitor their performance and validate the results of stress tests. Starvation was found to be unsuitable for larval quality evaluation. However, the impact of initial food deprivation on the newly hatched larvae indicates that feeding immediately after hatching is necessary for mud crab larvae. Exposure of larvae to 40 mg/L formalin for 3 hr appeared to be a reliable and practical method for larval quality assessment as the survival of larvae in the mass production tanks validated the classification of good and poor quality batches in the stress tests. On this basis, a hatchery operator can decide which batch should be cultured further. Finally, there appears to be a link between the quality of larvae and the performance at the megalopa and early juvenile crabs.     

基于PLC的蟹笼渔船诱饵填盒与塞笼自动化流水线方案设计

蟹笼渔船诱捕饵料在使用过程需要经过破冰分离、切块、填盒、塞笼等工序,效率低、劳动强度大。为提升蟹笼渔船的生产效率,需要实现饵料使用自动化。提出了一种实现饵料使用自动化的流水线生产方案,并通过Pro/ENGINEER Wildfire 5.0进行流水线的结构设计建模与原理分析,采用GX works3基于三菱FX5U系列PLC完成了流水线PLC控制梯形图程序设计,并在软件上进行在线模拟测试。结果显示:该流水线的结构设计理论上能满足蟹笼渔船饵料使用的自动化需求,控制系统的设计符合流水线的动作顺序控制,端口运行正常,未发生错误提示。该流水线生产方案的提出为蟹笼渔船诱捕饵料实现自动化提供了条件,同时也打破了蟹笼饵料的传统塞入方式,即由内部塞入改为外部挤入。控制系统的设计为蟹笼渔船诱捕饵料自动切块填盒与塞笼流水线的实际生产使用打下了理论基础。     

基于机器视觉的水下河蟹识别方法

为了探测河蟹在池塘中的数量及分布情况,为自动投饵船提供可靠的数据反馈,提出了基于机器视觉的水下河蟹识别方法。该方法通过在投饵船下方安装摄像头进行河蟹图像实时采集,针对水下光线衰减大、视野模糊等特点,采用优化的Retinex算法提高图像对比度,增强图像细节,修改基于深度卷积神经网络YOLO V3的输入输出,并采用自建的数据集对其进行训练,实现了对水下河蟹的高精度识别。实验所训练的YOLO V3模型在测试集上的平均精度均值达86. 42%,对水下河蟹识别的准确率为96. 65%,召回率为91. 30%。实验对比了多种目标检测算法,仅有YOLO V3在识别准确率和识别速率上均达到较高水平。在同一硬件平台上YOLO V3的识别速率为10. 67 f/s,优于其他算法,具有较高的实时性和应用价值。     

Characterization of Aquimarina hainanensis isolated from diseased mud crab Scylla serrata larvae in a hatchery

Mass mortality due to necrosis signs occurred in hatchery-reared zoea stage larvae of the mud crab Scylla serrata in Okinawa, Japan, and a causative bacterium was isolated. In this study, we identified and characterized the bacterium by genome analysis, biochemical properties and pathogenicity. The bacterium was a Gram-negative, non-motile, long rod, forming yellow colonies on a marine agar plate. It grew at 20–33°C (not at 37°C) and degraded chitin and gelatin. Phylogenetic analysis of the 16S rRNA gene sequence identified the bacterium as Aquimarina hainanensis. Genome sequence data obtained from Illumina MiSeq generated 29 contigs with 3.56 Mbp in total length and a G + C content of 32.5%. The predicted 16 chitinase genes, as putative virulence factors, had certain homologies with those of genus Aquimarina. Experimental infection with the bacterium conducted on larvae of four crustacean species, brine shrimp Artemia franciscana, freshwater shrimp Caridina multidentata, swimming crab Portunus trituberculatus and mud crab S. serrata, revealed that this bacterium was highly virulent to these species. The present study suggests that the bacterium caused mass mortality in mud crab seed production was A. hainanensis and can be widely pathogenic to crustaceans.     

Use of reflex indicators for measuring vitality and mortality of the snow crab (Chionoecetes opilio) in captivity

The snow crab (Chionoecetes opilio) is one of the most important commercial crabs in the world; it is heavily exploited in Atlantic Canada, Alaska, the Sea of Japan and the Barents Sea. Catches in the Barents Sea north of Norway have increased dramatically in the last decade. Most of the world's catch is processed, frozen and exported overseas. However, recently there has been considerable interest in exporting live snow crab, particularly in Norway. The stress of capture and live transport can result in significant mortalities. In order to establish a live export industry for snow crab, the welfare of the animal must be monitored throughout all the steps of the live transport process. In this study the reactions of snow crabs exposed to increasing periods of air exposure were measured in terms of reflex indicators, incidence of mortality, blood lactate levels and blood protein and haemocyanin. Although this is not a specific live holding or live transport process the aim was to test the suitability of reflex indicators to reflect vitality (stress) and not just to predict mortality. This would be compared with traditional blood biochemistry techniques for measuring crustacean stress. The study demonstrated that the reflex index score (RIS) is suitable to assess the vitality of snow crab. Longer air exposure periods render higher mortality rates and less vital individuals. The authors believe using vitality reflex indicators would be a suitable way of measuring crab welfare during the live holding and transport process.     

鱼蟹稻田飞虫捕食装置风光互补供能系统的研究

针对鱼蟹稻田飞虫捕食装置的离网型户外供能需求的问题，着重研究该装置的供能系统方案。基于户外供能合理性要求，采用小型风力发电机和太阳能电池板自主互补发电来进行能源供应；对小型风力发电机和太阳能电池板分别进行Matlab/Simulink建模，着重对叶轮的风能利用系数、叶尖速比和桨距角的相关性进行量化分析，利用单因素分析法对太阳能电池板进行分析；在桨距角确定的条件下，适当取值叶尖速比，能够获得最大风能利用系数。同时，在不同温度和辐射的条件下，太阳能电池板能够取得功率曲线的峰值，为风光互补的最大功率获取提供理论上的支持。针对离网型风光互补系统能源的合理化利用，提出混合供能的能量管理策略，符合该装置的供能需求，对户外同类型装置供能设计提供一定的参考。     

中国北方野生雄绒螯蟹成蟹可食率和生化成分分析

比较了2017年10月由我国绥芬河、图们江和辽河采集的质量为80~160 g的野生雄绒螯蟹成蟹的组织系数、总可食率、可食组织中一般化学组成及脂肪酸组成。试验结果显示:(1)3个种群雄体性腺指数、肝胰腺指数和出肉率差异显著,但3个种群总可食率无显著差异(P>0.05)。(2)就性腺而言,图们江种群水分含量最高,绥芬河种群脂肪含量最高,辽河种群蛋白质和灰分含量最高(P<0.05);辽河种群肝胰腺水分含量显著高于其他2个种群,但灰分含量显著低于其他2个种群,绥芬河种群肝胰腺中的脂肪含量最高(P<0.05);绥芬河种群肌肉的水分最低,蛋白质、脂肪和灰分含量最高,图们江种群肌肉中的脂肪含量最低,辽河种群肌肉的蛋白质和灰分含量最低。(3)绥芬河种群雄体性腺总长链多不饱和脂肪酸含量最高,图们江种群绒螯蟹肝胰腺和肌肉中总长链多不饱和脂肪酸含量最低(P<0.05);辽河种群的可食组织中n-3/n-6的值最低,图们江种群可食组织n-3/n-6的值最高。综上,绥芬河、图们江和辽河3个种群绒螯蟹野生雄体的总可食率无显著差异,但是可食部位的常规生化和脂肪酸含量存在显著差异。     

福建中南部近海四种主要作业蟹类种类组成及其分布特点

蟹类是福建海区重要的经济渔获种类之一。本文基于2009年1月-011年12月期间进行的蟹类调查资料，分析了福建中南部近海四种捕捞作业渔获物中蟹类的种类组成和分布特点。结果表明：福建中南部近海海域有蟹类94种，隶属16科、53属，以亚热带和热带的暖水性种为主，与南海关系最为密切，而与黄、渤海关系较为疏远。从蟹种分布区域看，这些蟹类在浅海均有分布，其中26．6％的蟹类既分布于浅海又分布于潮间带。从蟹种个体大小看，福建海区蟹类多数属于中小型种类。高强度捕捞加剧了对蟹类资源的破坏，因此需要调整捕捞作业结构，拓展外海蟹类捕捞作业，开展资源动态监测和苗种放流增殖工作，并实施TAC管理制度。