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1.
为探究琥珀酸黄杆菌(Flavobacterium succinicans)对非生物胁迫下多年生黑麦草(Lolium perenne)的缓解效应,本试验研究了菌株DSM4002的生物学特性,并在盆栽条件下,分析了干旱(维持相对含水量(30±5)%处理7 d和14 d)、低温(4℃处理3 d和7 d)和盐胁迫(150 mM NaCl处理5 d和10 d)下根际接种DSM4002对多年生黑麦草生长和生理特性的影响。结果表明,DSM4002具有分泌吲哚乙酸(Indole-3-acetic acid,IAA)、溶磷和产铁载体能力,对壮观霉素、卡那霉素等具有抗性,对氯霉素敏感。同时,接种DSM4002能够提高正常生长、干旱、低温和盐胁迫下多年生黑麦草的生物量、抗氧化酶活性、可溶性糖和脯氨酸含量,降低相对电导率和丙二醛(Malondialdehyde,MDA)含量。综上,琥珀酸黄杆菌促进多年生黑麦草的生长与其自身具有分泌IAA、溶磷和产铁载体能力有关,而且它能够调节植物体内氧化还原平衡,从而增强多年生黑麦草的抗旱、抗寒和抗盐能力。 相似文献
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为了研究铁调素调节蛋白(hemojuvelin,HJV)在硬骨鱼中抵御病原菌感染和维持自身铁稳态过程中的作用,实验扩增了尼罗罗非鱼铁调素调节蛋白基因(Onhjv)的开放阅读框(ORF),分析其在健康尼罗罗非鱼各组织中的分布模式及在抵御病原菌感染和调节铁稳态中的相关作用。结果显示,Onhjv的ORF全长由1 248个碱基组成,编码415个氨基酸,在不同物种之间具有一定的保守性。实时荧光定量PCR(qRT-PCR)结果显示,Onhjv在尼罗罗非鱼各组织中广泛分布,并在肝脏中的表达量最高。在无乳链球菌或嗜水气单胞菌感染后,Onhjv在肝脏、脾脏、肠和鳃中的表达量均显著上调。体外头肾单核/巨噬细胞和肝细胞中Onhjv表达量在受到这2种病原菌应激下也显著上调。此外,在1和10μmol/L FeCl_3溶液刺激后,Onhjv表达量在肝脏、脾脏、肠和鳃等组织,以及头肾单核/巨噬细胞和肝细胞中的表达量也呈显著上调。受重组罗非鱼IL-6蛋白[(r)OnIL-6]刺激后,头肾单核/巨噬细胞中Onhjv表达量显著上调,表明炎症因子可以促进Onhjv表达。研究表明,尼罗罗非鱼铁调素调节蛋白在宿主抵御病原菌感染和维持铁稳态的过程中发挥作用。本实验为探究HJV在硬骨鱼中的生物学功能提供了参考,同时为进一步研究铁代谢在宿主防御病原菌感染过程中的重要作用提供理论指导。 相似文献
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Wheat endosperm texture (hardness) largely determines end-product suitability. Since its development 25 years ago, the single kernel classification system (SKCS, a mechanical instrument that measures, among other properties, the force imparted on a kernel during crushing) has been used in breeding programs to differentiate soft wheats from hard wheats. Nominally, these have a soft to hard SKCS hardness index (HI) range of 25–75 (dimensionless units). However, in recent years, breeders have developed extremely soft (‘Super Soft’) lines having SKCS HI < 0. Until now, these very low SKCS HIs have not been corroborated with traditional methodologies that characterize mechanical strength. Herein, we report on the relationships between SKCS HI and three compressive strength properties (maximum stress, Young's modulus, and work) in Super Soft wheat. With respective correlation coefficients of 0.76, 0.66, and 0.75, we have found that the relationships between SKCS HI and compressive strength agree with prior research involving ordinary soft and hard wheats. 相似文献
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[Objective]The energy productivity potential of Saccharum spontaneum L.germplasm resources as energy plants was discussed in this paper.[Method]The energy productivity potential of Saccharum spontaneum L.germplasm resources collected from the wild was assessed from the aspects of cellulose,semicellulose,lignin and crude ash contents and caloric value.[Result]The total content of cellulose and semicellulose ranged from 41.75%to 69.13%,the lignin content ranged from 2.16%to 11.75%,the crude ash content ranged from 4.79%to 9.34%and the caloric value ranged from 16.00 to 17.69 MJ/kg among the 30 Saccharum spontaneum L.germplasms.Compared to other plants,Saccharum spontaneum L.had higher contents of cellulose and semicellulose,a higher caloric value but lower contents of lignin and crude ash.[Conclusion]From the perspective of producing fuel ethanol with lignocellulose,Saccharum spontaneum L.has a great potential for development as an energy plant. 相似文献
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Caroline Larsson Anne Vitger Rasmus B Jensen Peter Junghans Anne-Helene Tauson 《Acta veterinaria Scandinavica》2014,56(1)
Background
Overweight and obesity are the most common nutritional disorders in dogs and may lead to various secondary diseases and decreased lifespan. In obesity research, measurement of energy expenditure (EE) and determination of the energy requirements are essential. The objective with this study was to validate and evaluate the suitability of the oral 13C-bicarbonate technique (o13CBT) for measuring EE in dog obesity studies. A further objective was to investigate the impact of body weight (BW) reduction and changes in body composition on the EE when measured under conditions corresponding to the basal metabolic rate (BMR).Results
The EE in five privately owned, overweight dogs was measured simultaneously with the o13CBT and indirect calorimetry (IC) for comparison of the results. Two measurements per dog were performed under the same standardised conditions (i.e. fasted and resting state) at the start, and after completing a 12-week BW reduction program. Additionally, measurements of body composition by Dual-energy X-ray absorptiometry (DEXA) were conducted at the beginning and at the end of the BW reduction program. There were no differences in EE results obtained by the o13CBT and IC. Overweight and the BW reduction did not affect the estimates for the respiratory quotient (RQ) or the recovery factor for the 13C-tracer (RF), both needed when using the o13CBT. The dogs lost 16% (SD ± 2.0) of their initial BW in reduced fat mass (P < 0.001), whereas fat free mass (FFM) remained unchanged. There was no effect of the BW reduction on the determined EE expressed in kJ/kg BW/d, or in kJ/kg BW0.75/d. However, EE was lower (P < 0.001) after the BW reduction program when expressed in relation to FFM (kJ/kg FFM/d).Conclusions
Results from the present study show that the o13CBT can be a used in obesity research to determine EE in fasted dogs and under resting conditions. Furthermore, the results suggest that the BMR does not change with reduced BW in overweight dogs as long as the FFM remains unchanged. This indicates that the BMR to maintain one gram of fat is equal to maintaining one gram of FFM in overweight dogs. 相似文献9.
旅游业作为世界上最大的产业部门,其能源消耗和碳排放已经引起关注,其中旅游交通作为旅游业能源消耗和碳排放最主要的部门,许多学者对其进行了研究.科学全面地分析旅游交通能源消耗和碳排放有助于推动旅游交通的可持续发展,进而促进旅游业更健康地发展.分析了当前旅游交通能耗和碳排放的研究方法,然后从旅游交通能源消耗和碳排放的测度、影响因素、对环境的影响和响应对策等4个方面,综述了其主要的研究内容,最后对中国旅游交通能源消耗和碳排放的研究提出了建议. 相似文献
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