全文获取类型
收费全文 | 108篇 |
免费 | 12篇 |
国内免费 | 7篇 |
专业分类
农学 | 1篇 |
1篇 | |
综合类 | 50篇 |
农作物 | 1篇 |
水产渔业 | 61篇 |
畜牧兽医 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2016年 | 2篇 |
2015年 | 9篇 |
2014年 | 3篇 |
2013年 | 6篇 |
2012年 | 3篇 |
2011年 | 7篇 |
2010年 | 9篇 |
2009年 | 8篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 2篇 |
2005年 | 4篇 |
2003年 | 4篇 |
2002年 | 5篇 |
2000年 | 5篇 |
1999年 | 7篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1986年 | 4篇 |
排序方式: 共有127条查询结果,搜索用时 16 毫秒
1.
Snježana Zrnčić Dražen Oraić Ivana Giovanna Zupičić Željko Pavlinec Dragan Brnić Žaklin Acinger Rogić Ivica Sučec Dieter Steinhagen Mikolaj Adamek 《Journal of fish diseases》2020,43(6):673-685
Common carp (Cyprinus carpio) is a very important fish species for warm-water aquaculture in Croatia. All Croatian carp farms are subjected to a surveillance programme for the presence of koi herpesvirus (KHV), causing a deadly disease called koi herpesvirus disease (KHVD). However, there is no surveillance for other viral pathogens of importance like carp edema virus (CEV), a causative agent of koi sleepy disease (KSD). During regular testing within the KHVD surveillance programme, we tested samples for CEV simultaneously. The screening indicated possible outbreaks of KHVD and KSD. During 2016, KHVD broke out in an isolated area and soon thereafter a KHV eradication programme was successfully performed. However, during 2018 and 2019, two additional mortality events occurred in lakes in the southern part of Croatia during the spring. Samples from both events tested positive for CEV. An epidemiological investigation confirmed the introduction of infected carps from an infected farm to one of the lakes. To prevent the spreading of CEV into open waters, it is of utmost importance to introduce CEV testing before fish movement or to perform regular testing of all carp farms in the country to determine CEV prevalence for the purpose of implementation of control measures. 相似文献
2.
为研究水环境中Ni~(2+)对鲤鱼鳃和肝脏的组织损伤,将鲤鱼(Cyprinuscarpio)暴露在Ni~(2+)浓度为0、0.55、0.62、0.72、0.83、0.96、1.09、1.25mmol/L的水环境中进行急性毒性试验,并通过组织切片观察鳃和肝脏的组织病理变化。结果显示,Ni~(2+)对鲤鱼的96h半数致死浓度(LC_(50))为0.72 mmol/L,安全浓度(SC)为0.007 2 mmol/L。高浓度Ni~(2+)(1.25 mmol/L)试验组鲤鱼的鳃小片呼吸上皮细胞坏死、脱落,肝血窦淤血,肝细胞核固缩;中低浓度Ni~(2+)(0.72~1.09mmol/L)试验组鲤鱼的鳃小片呼吸上皮水肿浮离、细胞增生,血窦充血,肝细胞胞浆空泡化。表明水环境中的Ni~(2+)浓度高于0.007 2mmol/L时会对鲤鱼的鳃和肝脏造成急性毒性,导致鳃和肝脏出现明显的组织病理变化。 相似文献
3.
添加葡萄籽等植物提取物对湘云金鲫生长性能及鱼体成分的影响 总被引:1,自引:0,他引:1
选用1344尾平均体重为105 g的健康湘云金鲫鱼种。随机分为4个处理,每个处理2个重复。Ⅰ组投喂基础饲料,Ⅱ、Ⅲ、Ⅳ组在基础饲料中分别添加4 m g/kg的黄酶素及10、20 g/kg葡萄籽等植物提取物。30 d后测定湘云金鲫的生长性能和鱼体成分。结果显示:Ⅲ组与Ⅰ组、Ⅱ组、Ⅳ组比较,增重率分别提高53.77%、45.43%、20.16%(P<0.05),饵料系数分别降低33.06%、31.02%、16.44%(P<0.05);鱼体蛋白质含量显著提高(P>0.05),体脂肪含量无显著变化(P>0.05)。因此,葡萄籽等植物提取物作为饲料添加剂能显著提高湘云金鲫的生长性能、降低饵料系数、改善鱼体品质,本试验条件下,其适宜添加量为10 g/kg。 相似文献
4.
鲤鱼白细胞介素-1β全长cDNA的克隆·鉴定及其差异表达分析 总被引:1,自引:1,他引:0
[目的]进行鲤鱼白细胞介素-1β(IL-1β)全长cDNA的克隆、鉴定及其差异表达分析。[方法]利用DD-RTPCR方法获得差异表达cDNA片段,对有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库进行筛选,克隆了鲤鱼IL-1β的全长cDNA,并进行了序列分析和差异表达分析。[结果]获得的阳性克隆含有1个大小为831 bp编码276个氨基酸的完整开放阅读框。聚类分析表明,鲤鱼IL-1β氨基酸序列与日本鲤鱼紧密聚为一支,氨基酸序列的同源性达95%,之后聚类顺序依次为鲫鱼、斑马鱼、猪、牛、马、人和小鼠。差异表达分析表明,经有丝分裂原刺激后前期(4 h)白细胞中IL-1β的表达量显著增大,但随着时间推移(12,24 h)并非一直较同期大,表达量总体趋势成峰形。[结论]为进一步研究IL-1β在体内的表达方式、功能特点和调控机理以及在炎症反应、应急反应和免疫应答的作用机制奠定了基础。 相似文献
5.
A comparison between marine and terrestrial invertebrate meals for mirror carp (Cyprinus carpio) diets: Impact on growth,haematology and health 下载免费PDF全文
Invertebrate meals (e.g. polychaetes and insects) present novel and sustainable high‐quality nutrient sources for use in fish feed formulations. To test this innovative source, an eleven‐week feeding trial was conducted evaluating the effects of replacing the fishmeal (FM) component as an example of a superior protein source (FM CTRL) with ragworm meal (RW, Nereis virens) and/or silkworm pupae (SWP, Bombyx mori) in mirror carp (Cyprinus carpio) diets. Three experimental diets with partial replacement of FM (diets: RW + FM, SWP + FM and RW + SWP + FM) were formulated. All diets were formulated to be iso‐nitrogenous, iso‐lipidic and iso‐energetic. Growth performance and feed utilization indices were assessed, and the feeding trial concluded with the analysis of haematological parameters to provide an indication of carp physiological and health status. Mean weight gain was greatest in mirror carp fed RW + FM (60.83 fish?1 day?1; P < 0.05 vs. all other diets) followed by SWP + FM (40.62 g fish?1 day?1; P < 0.05 vs. all other diets). The least weight gain was achieved in fish fed FM + SWP + RW+ and FM CTRL (34.34 and 33.96 g fish?1 day?1, respectively; not significantly different from each other). Fish fed on RW + FM diet had significantly lower plasma ammonia concentrations than any other dietary groups (P = 0.04). Mirror carp fed on SWP + FM diet (111.52 units mL?1) were observed to have a marked enhancement in alternative complement activity than FM CTRL (79.21 units mL?1, P = 0.041). Both ragworm and silkworm pupae meal present attractive sustainable functional feed component in carp diets, with benefits on enhancing growth performance and specific physiological parameters. 相似文献
6.
甲砜霉素在鲤鱼中的药代动力学研究 总被引:1,自引:0,他引:1
本实验在(26±2)℃的养殖水温下,采用高效液相色谱–串联质谱法(HPLC-MS/MS)研究了以30 mg/(kg·bw)的剂量对鲤鱼(Cyprinus carpio)进行单次投喂药饵后甲砜霉素(Thiamphenicol,TAP)在鲤鱼体内的药物代谢动力学。通过DAS 2.0动力学软件分析TAP在鲤鱼体内的药–时数据,结果表明符合一级吸收二室模型。TAP在肌肉、肾脏、肝脏、鱼皮、鳃、脾脏和血浆各组织的药物达峰时间(T_(peak))分别为16、2、16、8、0、2和16 h,达峰浓度(C_(max))分别为15.6、35.3、12.4、9.0、33.0、11.6 mg/kg和21.0 mg/L;药–时曲线下面积(AUC)分别为1084.5、1578.1、777.3、541.1、0.1、478.1 mg/(kg·h)和485.1 mg/(L·h),消除半衰期(t_(1/2β))分别为11.4、100.2、54.2、41.1、69.5、38.0和71.9 h。TAP在鲤鱼体内各组织的分布和消除速率相差较大;在肾脏中的药物达峰时间短且达峰浓度高于其他组织,其消除半衰期也明显高于其他组织,推测肾脏是鲤鱼体内TAP蓄积和代谢的主要器官。按照农业部《动物性食品中兽药最高残留限量》文件规定,TAP在水产动物中最高残留限量(MRL)不得高于50μg/kg,本研究中,肌肉、肾脏、肝脏、鱼皮、脾脏和血浆的TAP残留量低于MRL的时间分别从第16、16、12、12、12、10和12天开始,将肌肉和肾脏作为TAP药物残留的靶组织,建议休药期不得低于16 d。 相似文献
7.
8.
染发废水对鲤鱼急性毒性试验研究 总被引:1,自引:0,他引:1
以鲤鱼为受试生物,将其暴露于不同浓度的染发废水中,通过半静止式试验,根据鲤鱼在各时间段的死亡率求出半致死浓度、安全浓度以及毒性累积程度系数等特征参数,探讨染发废水对鲤鱼的急性毒性,评价染发废水的毒性等级。结果表明:染发废水急性毒性等级为中等毒;染发废水在48 h内稳定性较差,48 h后才能趋于完全稳定;染发废水对鱼类急性毒性试验时间至少可缩短至48 h。 相似文献
9.
To assess strain related differences in growth performance and growth patterns under the same culture environment, four strains of common carp, two each of the scale carp, Cyprinus carpio var. communis (Chinese big-belly carp and long bodied carp) and mirror carp, C. carpio var. specularis (scattered carp and linear carp) were communally stocked in three fertilized earthen ponds of 0.14 ha each at 5,000 fish ha?1 in the ratio of 1:1:1:1 during an 11-month (February to December) culture cycle. Chinese big-belly carp grew larger than other groups, among which there were no significant differences. Scale carp strains performed relatively better than mirror carp at higher temperatures and then essentially stopped growing as temperatures declined into winter. The strains of mirror carp, on the other hand continued growing well later into the cold season. 相似文献
10.
CaCl2对鲫鱼血清Ca^2+浓度的影响 总被引:1,自引:0,他引:1
鲫鱼体腔注入0.68MCaCl2后2-16小时血清Ca^2+浓度显著高于正常对照组,但其数值随时间的延长而逐渐降低,16小时后继续降低,到48小时其值显著低于正常对照组,低血钙状态持续到96小时,我们认为Ca^2+浓度的这种变化特征与斯坦尼小体的功能密切相关。 相似文献