排序方式: 共有32条查询结果,搜索用时 15 毫秒
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青山羊精子中乳酸脱氢酶同工酶的研究 总被引:1,自引:0,他引:1
用琼脂糖凝胶电泳技术,对山东青山羊精子中LDH同工酶进行研究。结果表明,9只青山羊精子中均检出4条LDH同工酶谱带。即LDH-1、LDH-3、LDH-4和LDH-x。LDH-x酶带位于LDH-4和LDH-5之间,电泳迁移率近于LDH-5,含量为LDH的75%,65℃处理5min稳定。本文结合LDH-x同工酶特殊功能,对其生理意义和临床意义进行讨论。 相似文献
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Gama FG Santana AE Filho Ede C Nogueira CA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(1):85-88
BACKGROUND: Cerebrospinal fluid (CSF) is produced in the cerebral ventricles through ultrafiltration of plasma and active transport mechanisms. Evaluation of proteins in CSF may provide important information about the production of immunoglobulins within the central nervous system as well as possible disturbances in the blood-brain barrier. OBJECTIVE: The objective of this study was to measure the concentration and fractions of protein in CSF samples using a membrane microconcentrator technique followed by electrophoresis, and to compare the protein fractions obtained with those in serum. METHODS: CSF samples from 3 healthy dogs and 3 dogs with canine distemper virus infection were concentrated using a membrane microconcentrator having a 0.5 to 30,000 d nominal molecular weight limit (Ultrafree, Millipore, Billerica, MA, USA). Protein concentration was determined before and after concentration. Agarose gel electrophoresis was done on concentrated CSF samples, serum, and serial dilutions of one of the CSF samples. RESULTS: Electrophoretic bands were clearly identified in densitometer tracings in CSF samples with protein concentrations as low as 1.3 g/dL. The higher CSF protein concentration in dogs with distemper was mainly the result of increased albumin concentration. CONCLUSION: The microconcentrating method used in this study enables characterization of the main protein fractions in CSF by routine electrophoresis and may be useful for interpreting the underlying cause of changes in CSF protein concentrations. 相似文献
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不同凝胶电泳对玉米自交系DNA多态检测的影响 总被引:1,自引:0,他引:1
利用10个SSR(简单序列重复)引物对,对10个玉米自交系进行同源位点扩增,用琼脂糖凝胶及聚丙烯酰胺凝胶两种电泳方法分离,检测其DNA多态性。结果表明,其间的多态性检测结果差异较大。聚丙烯酰胺凝胶电泳较琼脂糖凝胶电泳能检出更多的等位基因位点,多态信息含量值亦较高,自交系间遗传相似系数的变幅小于琼脂糖凝胶检测中的相应值。聚丙烯酰胺凝胶电泳的分辨率高于琼脂糖凝胶电泳,对自交系的系统聚类更为准确、可靠,更能真实地反映自交系间的系谱关系。 相似文献
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为了筛选适宜于甜菜品种纯度鉴定的DAMD引物,笔者利用12 个不同的甜菜品种分别对26 条DAMD引物进行扩增,并分别采用6%聚丙烯酰胺凝胶电泳和2%的琼脂糖凝胶电泳对扩增产物进行检测。结果表明,从26 条DAMD引物中筛选出16 条扩增清晰的引物,这16 条引物共扩增出139 条带,其中多态性条带为122 条,多态性百分比为87.7%,这16 条引物可以作为甜菜品种纯度和真实性鉴定的核心引物,同时发现聚丙烯酰胺凝胶电泳与琼脂糖凝胶电泳的检测结果差异不大,而琼脂糖凝胶具有制作方便、检测简单以及不使用任何有毒药剂等优点,推荐甜菜DAMD扩增产物的检测使用琼脂糖凝胶电泳。 相似文献
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Crivellente F Bonato M Cristofori P 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):73-78
BACKGROUND: Serum protein analysis in both humans and experimental animal species has so far been carried out by labor-intensive techniques, such as agarose gel electrophoresis (AGE). OBJECTIVE: The objective of this study was to evaluate capillary electrophoresis (CE) as an alternative technique to AGE for the analysis of serum proteins from healthy animals. METHODS: Blood samples were collected into tubes without anticoagulant from 6 fasted healthy male mice, rats, dogs, marmosets, and humans. Serum proteins were separated by CE using a technique standardized for the analysis of human proteins, and the results (efficiency, resolution, and precision) were compared with those obtained through AGE. RESULTS: Compared with AGE, CE resulted in narrower peaks and more peaks. The efficiency of protein separation by CE was significantly higher for all species, and resolution (R) was significantly higher in samples from dogs. Using rat serum, intraday reproducibility was lower for all protein fractions, and interday reproducibility was lower for most peaks, compared with AGE. CONCLUSIONS: We conclude that CE is a viable alternative to AGE for the determination of protein electrophoresis in a routine veterinary clinical pathology laboratory. The minimal sample requirement (2 microL), complete automation, and quantitative results make CE an especially valuable technique for protein analysis in experimental animal models. 相似文献
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黄瓜SRAP-PCR反应体系的建立 总被引:7,自引:1,他引:6
为了建立黄瓜适宜的SRAP(相关序列多态性)反应体系,以长形白皮少刺黄瓜B-2-2和圆形白皮黄瓜Y-3为材料,分析了不同来源的Taq酶及其浓度、Mg2 浓度、dNTP浓度、DNA模板浓度、引物浓度对扩增结果的影响,并比较了琼脂糖凝胶和变性聚丙烯酰胺凝胶电泳检测扩增产物多态性的差异。结果表明,在12.5μL反应体系中,Taq酶适用Takara酶,其最适用量为0.5U;DNA模板最适浓度为30 ng,Mg2 最适浓度为2.0 mmol/L,引物最适浓度为0.96μmol/L,dNTP最适浓度为200μmol/L。6%的变性聚丙烯酰胺电泳检测扩增产物效果比琼脂糖检测效果好。用不同黄瓜材料的基因组DNA两次SRAP-PCR扩增,6对引物均能扩增出清晰且重复性好的谱带。因而建立的黄瓜SRAP反应体系稳定可靠。 相似文献