Evaluation of the Effect of Sprout Soybeans on the Iron Status of Anemic Adolescent Girls in Rural China

Plant Foods for Human Nutrition - Soybeans are a major source of nonheme iron in Chinese diet. Germination is considered to be effective in improving iron bioavailability in soybeans. The study is...     

抗AIV(H9)独特型杂交瘤细胞株检测方法的建立

采用兔抗AIV IgG作为检测抗原,通过预试确定了包被抗原的工作浓度为800μg／mL,以间接ELISA 方法检测融合细胞培养上清液,筛选到产生抗AIV鸡和兔种间共有独特型抗体的杂交瘤细胞,间接ELISA试 验中P／N值达12,证明杂交瘤细胞分泌目的单克隆抗体旱现强阳性。运用该检测方法,排除了分泌抗鸡同种 型表位以及超变区其他表位抗体的杂交瘤细胞,从而为筛选分泌抗H9亚型AIV独特型单克隆抗体的杂交瘤 细胞确定了可靠的方法。     

改进的QuEChERS-高效液相色谱-串联质谱法同时测定蔬菜水果中百菌清及其代谢物残留

建立了同时检测蔬菜水果中百菌清及其代谢物4-羟基百菌清的高效液相色谱-串联质谱分析方法。样品经酸化乙腈提取后,采用配备大气压化学电离源的三重四极杆串联质谱仪测定。结果表明:百菌清及其代谢物4-羟基百菌清在0.005~0.2 mg/L范围内峰面积与质量浓度的线性关系良好,决定系数（R2）大于0.99;方法定量限为0.01 mg/kg。在0.01、0.05和0.2 mg/kg 3个添加水平下,百菌清及其代谢物4-羟基百菌清在结球甘蓝、黄瓜、葡萄和苹果4种基质空白样品中的平均回收率为83%~104%,相对标准偏差（RSD）为 0.73%~7.7% (n=6)。该方法简单快速、准确可靠,适合蔬菜水果中百菌清及其代谢物4-羟基百菌清的定量测定。     

霜霉威吡虫啉腐霉利在结球生菜上的消解动态及相关因子的影响

通过开展结球生菜生产中所用主要农药--霜霉威、吡虫啉、腐霉利的消解规律研究,探讨生菜农药消解与农药种类、浓度及外部因子的关系,为结球生菜生产过程中的安全管理提供参考依据。以目前我国生产上主要使用的结球生菜品种--＂皇帝＂为材料,2007年春季露地种植,按霜霉威、腐霉利、吡虫啉最大推荐用量（计算浓度分别为1203.3、500.0、40.0mg·kg-1）和最大推荐用量的2倍（计算浓度分别为2406.6、1000.0、80.0mg·kg-1）两个农药剂量处理,分别于6月6日施药后当日、2、6、9d取样测定农药残留含量,并记录环境因子温度、光照的变化。结果表明,不论是按最大推荐用量还是最大推荐用量2倍喷施,吡虫啉、霜霉威、腐霉利在生菜上均表现为起始和最后阶段消解快、中间阶段消解平缓的特点;按日本肯定列表制度对生菜吡虫啉、霜霉威和腐霉利最大残留限量（分别为5.0、10.0和5.0mg·kg-1）要求,对于吡虫啉,即使按最大推荐用量2倍喷施,其喷施8h后的残留量仅为1.5mg·kg-1,说明春季露地生菜上喷施吡虫啉的安全性较高;对于霜霉威,按最大推荐用量喷施,施药6d后的残留量为10.7mg·kg-1,接近肯定列表制度的要求,施药9d后的残留量降至8．4mg·kg-1,表明霜霉威自喷药至采收的间隔期应至少在7d以上才可能是安全的;对于腐霉利,到试验结束时,不论何种喷施浓度,其残留量均超过5．0mg·kg-1的要求,其使用安全性需要引起重视。在影响吡虫啉、霜霉威、腐霉利在生菜上消解的因素方面,统计分析表明,外部因子（时间、温度、光照）对3种农药消解具有显著作用（P〈0．05）,农药种类在对其消解率影响方面达到极显著差异（P〈0．01）,其中吡虫啉最易消解,其次为霜霉威,腐霉利消解最慢,但3种农药喷施浓度的高低对各自消解率均无显著影响（P〉0．05）。     

酒类酒球菌的批式与流加培养特性

在葡萄酒后发酵过程中,酒类酒球菌(Oenococcus oenos)被认为是最主要的苹果酸—乳酸转化微生物,选育并制备价格低廉的优质活性酒类酒球菌菌剂,用于葡萄酒后熟,对葡萄酒的品质改良具有重要意义。通过对酒类酒球菌的培养,考察了不同培养基组成下的批式及流加培养对细胞生长特性的影响。结果表明,3种培养基中,以番茄汁培养基批式培养的菌浓度最大,达到了14×108cfu/mL;ATB培养基次之,为3.3×108cfu/mL;MRS最小,为2.5×108cfu/mL。通过在3种培养基中流加高浓度葡萄糖培养基,来维持培养基的初始糖浓度的批式培养,可以较有效提高细胞培养密度约2倍。     

夏季莱州湾及附近水域理化环境及营养现状评价

根据2007年8月对莱州湾及附近水域的调查资料，讨论了温度、盐度、pH、溶解氧（DO）、溶解无机氮（DIN）和活性磷酸盐（PO4^3--P）等理化因子的分布特征，并对该水域的营养水平和有机污染状况进行了评价。结果表明，莱州湾西部和西南部水域表层和底层的DIN含量较高，均呈向东北方向递减的趋势；表层PO4^3--P含量分布较为均匀，在湾口中部水域有一小范围的高值区，并呈向四周逐渐递减分布。底层水平基本与表层一致，但其高值区范围较大，等值线较为密集。营养盐与盐度的相关分析表明，DIN与盐度呈显著负相关，表明DIN主要来自河流径流的输送；而PO4^3--P与盐度相关性不十分显著，这表明莱州湾PO4^3--P的补充并非主要来自河流径流输入，而是以有机物的分解矿化再生为主，同时，浮游植物的大量繁殖也是一个重要的影响因素。由水域环境质量评价结果看，N/P值均大于Redfield比值16，P显得相对缺乏。根据营养化评价模式和有机污染指数计算结果，2007年夏季莱州湾水域营养水平基本属于磷中等限制潜在性富营养，有机污染程度属3级，表明该调查水域受到轻度有机污染。     

Changes in cell adhesion molecules and composition of complement activation in patients with acute myocardial infarction

AIM: To explore the possible changes in cell adhesion molecules and composition of complement activation in patients with acute myocardial infarction (AMI). METHODS: The expression of leukocyte CD18, soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular-cell adhesion molecule-1 (sVCAM-1) and composition of complement activation (sC5b-9) concentrations of patients with AMI (67 cases), old myocardial infarction (OMI, 42 cases) and 38 healthy volunteers were measured using enzyme-linked immunosorbent assay(ELISA). RESULTS: The expression of leukocyte CD18, sICAM-1,sVCAM -1 and sC5b-9 were significantly higher in AMI patients than that in normal controls and OMI patients(P＜0.01). Interestingly, it was also found that the expression of leukocyte CD18, sICAM-1,sVCAM-1 and sC5b-9 concentrations were much higher in patients with ventricular arrhythemia (VA) and the died than that in patients without VA and survivals (P＜0.01). Furthermore, the leukocyte CD18 expression, sICAM-1 and sVCAM-1 were positively correlated to sC5b-9 in AMI patients (r=0.648,0.652,0.668,0.698,0.914,0.725,0.737,0.752,0.792,P＜0.01),and leukocyte CD18 expression was positively correlated to sICAM-1 and sVCAM-1(r=0.662,0.683,0.695,0.738,0.744,0.745, P＜0.01).CONCLUSION:The interaction of cell adhesion molecules and composition of complement activation might participate in the occurance and development of AMI,and closely related to the seriousness of patients'condition and prognosis.     

Change of Gq-phosphoinositide signaling pathway in left ventricular tissue in rats with chronic heart failure and reverse effect of benazepril on it

AIM: To investigate the changes of Gq-phosphoinositide pathway in left ventricular tissue of rats with chronic heart failure in order to assess the role of this signal pathway in the formation of heart failure. METHODS: Male Sprague-Dawle rats were divided into three groups: control, chronic heart failure and benazepril therapy group. Chronic heart failure was induced with adriamycin. Rats in benazepril group received benazepril 10 mg·kg-1·d-1 and adriamycin at the same time. Hemodynamic measurement was carried out after 4 weeks. The expression of Gα q/11 protein in left ventricle was detected by Western blotting analysis and activity of phospholipase C was measured by the method of hydrolysis of nuclear substrate. RESULTS: Compared with control group, the ±dp/dtmax in chronic heart failure group significantly decreased, and protein Gα q/11 expression, basic and stimulated phospholipase C activity significantly increased (P<0.01). The ±dp/dtmax in benazepril group was significantly lower than that in control but obviously higher than that in chronic heart failure group (P<0.05). Gα q/11 expression, basic and stimulated phospholipase C activity in benazepril group were significantly higher than those in control but obviously lower than those in heart failure group (P<0.01). CONCLUSION: Gq-phosphoinositide signaling pathway may play a role in the formation of chronic heart failure. Benazepril partialy attenuates the activation of phosphoinositide signaling pathway.     

Development of Microsatellite Markers in Sea Perch,Lateolabrax Japonicus,from Codominant Amplified Fragment Length Polymorphism Bands

A fast and cost‐effective protocol to develop candidate microsatellite markers from sea perch, Lateolabrax japonicus, was described here. Ten suites of codominant bands that contained seven microsatellites were discovered in this marine fish, in which no microsatellite development was reported previously. All the seven microsatellites were found to be polymorphic among tested 20 individuals of sea perch. Five of the above seven loci conformed to Hardy–Weinberg equilibrium (HWE) as determined by using the Markov‐Chain method implemented, the other two loci significantly deviated from HWE, both of them showed a large heterozygote excess (H_E). Out of 21 possible pair‐wise comparisons among the seven loci applied to sea perch, none showed significant linkage disequilibrium (LD) (P > 0.008). Five additional fish species assessed for cross‐species amplification revealed that some of the loci appear to be applicable in close‐related species.     

霜霉威吡虫啉腐霉利在结球生菜上的消解动态及相关因子的影响

通过开展结球生菜生产中所用主要农药——霜霉威、吡虫啉、腐霉利的消解规律研究,探讨生菜农药消解与农药种类、浓度及外部因子的关系,为结球生菜生产过程中的安全管理提供参考依据。以目前我国生产上主要使用的结球生菜品种——皇帝为材料,2007年春季露地种植,按霜霉威、腐霉利、吡虫啉最大推荐用量(计算浓度分别为1203.3、500.0、40.0mg·kg-1)和最大推荐用量的2倍(计算浓度分别为2406.6、1000.0、80.0mg·kg-1)两个农药剂量处理,分别于6月6日施药后当日、2、6、9d取样测定农药残留含量,并记录环境因子温度、光照的变化。结果表明,不论是按最大推荐用量还是最大推荐用量2倍喷施,吡虫啉、霜霉威、腐霉利在生菜上均表现为起始和最后阶段消解快、中间阶段消解平缓的特点;按日本肯定列表制度对生菜吡虫啉、霜霉威和腐霉利最大残留限量(分别为5.0、10.0和5.0mg·kg-1)要求,对于吡虫啉,即使按最大推荐用量2倍喷施,其喷施8h后的残留量仅为1.5mg·kg-1,说明春季露地生菜上喷施吡虫啉的安全性较高;对于霜霉威,按最大推荐用量喷施,施药6d后的残留量为10.7mg·kg-1,接近肯定列表制度的要...