Study on the Comparison of Different Methods of Simplifying Capacity Spectrum

It is necessary to simplify the capacity spectrum into bilinear form so as to get characteristic point of hysteretic bone curve(especially the yield point and the yielded stiffness),ductility and equivalent damping of the structure.Three general methods of calculating yield point and yielded stiffness of equivalent SDOF are introduced and the yield point and the yielded stiffness of three frame structures are compared using above given methods in this paper.The hysteretic bone curves of equivalent SDOF are decided by the equivalent yield force and displacement,the maximum plastic displacements of equivalent SDOF under rare earthquake are calculated,and it is compared with the maximum plastic displacements of member structures using nonlinear dynamic analysis.Finally,the advice of simplifying capacity spectrum is advanced.     

实时荧光定量PCR检测凡纳滨对虾和罗氏沼虾卵黄蛋白原mRNA在卵巢和肝胰腺中的表达

卵黄磷蛋白作为卵黄蛋白的主要成分, 可为甲壳动物胚胎和早期幼体发育提供能量, 为研究其来源及合成规律, 实验应用SYBR GreenⅠ荧光定量PCR法检测了凡纳滨对虾和罗氏沼虾性腺不同发育时期卵巢和肝胰腺两种组织中卵黄蛋白原mRNA的表达水平。结果发现,凡纳滨对虾和罗氏沼虾的卵巢和肝胰腺中都有卵黄蛋白原mRNA的表达。随着卵巢的发育, 凡纳滨对虾卵巢中卵黄蛋白原mRNA的相对表达量在前5个阶段不断增加, 分别为1.1, 5.9, 10.4, 26.9, 85.0, 恢复期急剧减少, 为1.6。肝胰腺中的相对表达量也不断增加, 分别为1.3, 3.3, 7.1, 37.3, 51.6, 恢复期急剧减少, 为1.0。罗氏沼虾肝胰腺中卵黄蛋白原mRNA的相对表达量在前四个阶段不断增加, 分别为3.4, 12.6, 15.2, 38.9, 抱卵期急剧减少, 为2.9;而卵巢在整个发育过程中对卵黄蛋白原合成的贡献比较小, 分别为1.0, 1.3, 1.7, 4.8, 1.5。研究表明, 两种虾类的肝胰腺和卵巢均具合成卵黄蛋白的功能, 而且在不同的卵巢发育阶段呈现明显的规律性。