Microscopic detection of reactive oxygen species generation in the compatible and incompatible interactions of Alternaria alternata Japanese pear pathotype and host plants

 Reactive oxygen species (ROS) generation was examined in the interaction of Alternaria alternata Japanese pear pathotype and host plants using three methods: nitro blue tetrazolium (NBT) method for microscopic detection of O₂ ⁻, diaminobenzidine (DAB) methods for microscopic detection of H₂O₂, and cerium chloride methods for ultrastructural detection of H₂O₂. ROS generation was detected by NBT and DAB methods at appressoria on leaves of susceptible cultivars and heat-shocked leaves of resistant cultivars but not in leaves of resistant cultivars. Ultrastructural detection by the cerium chloride method identified ROS generation at cell walls of appressoria and penetration pegs in susceptible, resistant leaves and heat-shocked leaves. These differences in the ultrastructural and microscopic data in resistant areas were due to the restriction of ROS generation in limited areas, the side facing the plant surface, of appressoria and penetration pegs. Therefore, ROS generation was apparently induced regardless of the resistance or susceptibility of the cultivar with the difference being in the volumes generated. After evaluating the pathological role of ROS generation in fungal structures, such generation was found to be associated with early penetration of cell walls in pear plants. Additionally, ROS generation in plants was also found in degrading pectin layers near infected hyphae and in plasma membrane modification sites in susceptible leaves but not in resistant leaves. ROS generation in susceptible leaves might be accompanied with plasma membrane damage, although the role of ROS generation in the pectin layers is not clear. ROS generation in both fungal and plant cells during their interaction was likely associated with the expression of susceptibility. Received: June 3, 2002 / Accepted: July 31, 2002     

Numerical Simulation for B900FD-1 Bulletproof Armor Plate Against Penetration

ANSYS/LS-DYNA is used to simulate the penetration process of B900FD - 1 bulletproof armor plate, Comparisons and analysis were made between numerical simulation and experiment results. The numerical simulations shows that the Johnson-Cook constitutive model could simulate the physical and mechanical behaviors of ductile materials under shock-loaded. The ultimate-thickness of B9OOFD-1 bulletproof armor plate under 500m/s impact velocity is analyzed by more numerical simulations.     

Botryosphaeria dothidea在苹果果实上的侵染过程(英文)

对Botryosphaeria dothidea通过皮孔侵入果实的过程进行了光学显微镜和扫描电镜观察,发现接种后2d分生孢子完成萌发和附着胞形成过程。接种后10d,菌丝体在皮孔表面缓慢生长。从20￣30d菌丝体加速扩展并形成几个分枝,通常从皮孔外围侵入果实,但仅限于皮孔表面组织。从40～50d,菌丝体扩展更为繁茂,一些菌丝开始突入皮孔的第2层组织。接种皮孔中可检测到多聚半乳糖醛酸酶(PG),但活性比对照高。皮孔有3层,第1层为含果胶的死组织,易被病菌侵染降解,底部的第3层非常坚固,是阻止病菌侵染的有效屏障。随果实发育,皮孔大小、数目和裂口均有规律地增加。我们的研究结果进一步证实了苹果轮纹病的潜伏侵染特性。     

Calcium ion promotes successful penetration of powdery mildew fungi into barley cells

To determine whether Ca²⁺ promotes powdery mildew penetration, Ca²⁺-treated barley coleoptiles were inoculated with conidia of pathogenic and nonpathogenic fungi. Penetration efficiency of the pathogenic powdery mildew Blumeria graminis was enhanced by Ca²⁺ treatment, but that of the necrotrophic pathogen Helminthosporium sp. remained unaffected. Similarly, when actin-dependent penetration resistance is suppressed with cytochalasin A, Ca²⁺ treatment specifically enhanced penetration of the nonpathogenic powdery mildew Erysiphe pisi but not that of other nonpathogens. Calmodulin inhibitors suppressed the promotive effect of Ca²⁺ on B. graminis penetration. These results suggest that barley powdery mildew specifically requires Ca²⁺ and calmodulin for penetration.     

助剂对高效氯氰菊酯在甘蓝叶片表皮渗透性的影响

 研究了3类助剂（矿物油、高级脂肪酸、非离子表面活性剂）对非内吸性杀虫剂高效氯氰菊酯在甘蓝叶片表皮渗透性的影响,证明药液的渗透性在一定范围内与药剂中助剂含量和给药后的时间成正相关。助剂间一定比例的配合对该渗透性具有协同作用。通过添加助剂后药液的各种物理性状进行测试及助剂溶液改变甘蓝表面蜡质结构的电镜观察,初步讨论了各类助剂影响渗透性的作用机理。     

Changes in penetration resistance of Ultisols from southern China as affected by shearing

Shear stresses and soil properties modified due to stress play an important role during formation of seals in a series of rainfall events and during tillage. The objectives of the study were to evaluate the effects of the penetrometer geometry on the penetration resistance as affected by shearing under different initial soil conditions and to use the information on soil strength to elucidate shearing process. Nine homogenous air-dried soils (<2 mm) were sprayed and stored so as to obtain equilibrium soil water contents. The moist soils were sheared by horizontal displacement of layers of soil particles/aggregates in between hands in one direction. The soil cores were prepared with comparable bulk density before the measurement of maximum penetration resistance (P_max) with a small flat tip and a cone tip penetrometers. At a wide range from 0.05 to 6.2 MPa, P_max was linearly correlated between the small flat tip and the cone tip penetrometers. The conversion ratio was higher under the saturation condition irrespective of the shearing effect. The penetrometer with the small flat tip was more sensitive for the weak soils. Shearing generally increased P_max in most cases, but it decreased P_max for some sandy soils under both saturated and unsaturated conditions and for a clayey soil under the saturated condition. The soil consisting of swelling clay exerted a decrease in P_max. Rearrangement and/or sliding of particles/aggregates and increase in soil suction during shearing were attributed to the increase in P_max. Increase in porosity due to the aggregation during shearing was ascribed to the decrease in P_max. In addition, it was shown that agricultural cultivation resulted in a reduction in soil strength.     

Hydro-physical responses of gypseous and non-gypseous soils to livestock grazing in a semi-arid region of NE Spain

Pasture productivity depends on soil hydro-physical properties, which in turn are deeply affected by livestock grazing. However, the comparative response of different soil types, and particularly gypseous soil types, to grazing has hardly been studied before. This paper compares the effect of grazing on the soil hydro-physical properties of silty gypseous (Gy) and non-gypseous (NGy) soils located in a semi-arid region (Middle Ebro Valley, NE, Spain). Two different soil managements were selected: ungrazed natural shrubland (N) and grazed shrubland (GR) soils. The gypsum, CaCO₃ and organic matter content (OM), soil texture, soil bulk density (ρ_b), penetration resistance (PR), saturated sorptivity (S), hydraulic conductivity (K), and the water retention curve (WRC) for undisturbed soil samples from 1 to 10 cm depth soil layer were measured. The ρ_b and PR in NGy soils were significantly higher than those observed in the Gy ones. Soil compaction due to grazing treatment tended to increase ρ_b and decrease the K and S values. While no differences in PR were observed in the Gy soils between grazing treatments, the PR measured in the NGy soils under GR was significantly higher than the corresponding values observed under N. Differences in K and S between GR and N treatments were only significant (p < 0.05) in NGy soils, where K and S values under the N treatment were almost four times greater than the corresponding values measured under GR. Overall, no differences in the WRCs were observed between soil types and grazing treatments. While the WRCs of NGy soils were not significantly affected by the grazing treatment, Gy soils under N treatment present a significantly higher level of soil macropores than under GR treatment. The hydro-physical features of Gy soils tended to be less affected by grazing than those of the NGy soils. These results suggest that livestock grazing, in both Gy and NGy soils, has a negative effect on the physical soil properties, which should be taken into account by land managers of these semi-arid regions where silty gypseous and non-gypseous areas coexist.     

Root growth conditions in the topsoil as affected by tillage intensity

Many studies have reported impeded root growth in topsoil under reduced tillage or direct drilling, but few have quantified the effects on the least limiting water range for root growth. This study explored the effects of tillage intensity on critical soil physical conditions for root growth in the topsoil. Samples were taken from a 7-year tillage experiment on a Danish sandy loam at Foulum, Denmark (56°30′ N, 9°35′ E) in 2008. The main crop was spring barley followed by either dyer's woad (Isatis tinctoria L.) or fodder radish (Raphanus sativus L.) cover crops as subtreatment. The tillage treatments were direct drilling (D), harrowing 8-10 cm (H), and ploughing (P) to 20 cm depth. A chisel coulter drill was used in the H and D treatments and a traditional seed drill in the P treatment. Undisturbed soil cores were collected in November 2008 at soil field moisture capacity from the 4-8 and 12-16 cm depths.We estimated the critical aeration limit from either 10% air-filled porosity (ε_a) or relative gas diffusivity (D/D₀) of 0.005 or 0.02 and found a difference between the two methods. The critical limit of soil aeration was best assessed by measuring gas diffusivity directly. Root growth was limited by a high penetration resistance in the D and H soils (below tillage depth). Poor soil aeration did not appear to be a significant limiting factor for root growth for this sandy loam soil, irrespective of tillage treatment. The soil had a high macroporosity and D/D₀ exceeded 0.02 at field capacity. Fodder radish resulted in more macropores, higher gas diffusivity and lower pore tortuosity compared to dyer's woad. This was especially important for the H treatment where compaction was a significant problem at the lower depths of the arable layer (10-20 cm depth). Our results suggest that fodder radish could be a promising tool in the amelioration of soil compaction.     

Für eine Verdopplung der Ca-Gehalte der Äpfel sind auch unter günstigen Bedingungen mindestens 12 Spritzungen mit Kalziumchlorid erforderlich

Zusammenfassung Siebenjährige Apfelbäume der Sorten Elstar, Gala, Karmijn de Sonnaville, Pinova und Pilot wurden 2003 von Juni bis September 12-mal mit 400 l/ha CaCl₂-Lösungen gesprüht. Kontrollen (Fu) waren Bäume, die nur mit Fungiziden (Discus, Dithane Ultra oder Kumulus) behandelt wurden. Bei den Behandlungen FuCa wurde den Fungiziden CaCl₂ (ansteigend von 5–25 g/l) zugegeben. Die Behandlungen FuCaTe enthielten zusätzlich das Netzmittel Plantacare 1200 UP, um die Benetzung der Früchte (Oberflächenspannung 29–30 mN/m) zu verbessern. Die Ca-Konzentrationen in den Kontrollfrüchten (Schale plus 5 mm Fruchtfleisch) variierten von 3,6 (Elstar) bis 9,0 mg/100 mg Frischsubstanz (Pilot). Durch die Fruchtdüngung (FuCa) stiegen die Konzentrationen auf 6,3 bzw. 13,3 mg/100 g Frischsubstanz (FS). Zugabe des Netzmittels (FuCaTe) erhöhte die Ca-Gehalte weiter auf 6,8 bzw. 14,9 mg/100 g FS. Bezogen auf die Behandlung Fu entspricht das einer Erhöhung der Ca-Konzentrationen der Früchte auf 163 bzw. 193%. Der Tensideffekt auf die Ca-Konzentrationen der Früchte wird auf die schnellere Penetration des CaCl₂ infolge besserer Benetzung zurückgeführt, da ein Einfluss auf die Retention der Spritzbrühe ausscheidet. Von der applizierten Ca-Dosis in Höhe von 26 kg/ha wurden nur 2,3 kg in den Früchten (50 t/ha) wieder gefunden, was einer Wiederfindungsrate von 8,8% entspricht. Die beobachtete Erhöhung der Ca-Konzentrationen der Früchte durch 12 Spritzungen dürfte in der Nähe des maximal Möglichen liegen, da die Witterungsbedingungen günstig und die Ca-Konzentrationen der Spritzbrühen hoch waren.