大白菜细胞质雄性不育的分子鉴定及序列分析

为了获得3种大白菜细胞质雄性不育系Ogu CMS,Pol CMS,CMS96和保持系间的多态性以及定位大白菜CMS96不育系所属的不育类型,利用设计的atp6,orf222单一和混合引物PCR扩增3组11份同核异质大白菜细胞质雄性不育系和保持系mtDNA。结果表明,atp6引物在大白菜Ogu CMS不育系扩增的200 bp片段为其特异带;orf222引物仅在大白菜Pol CMS和CMS96不育系有扩增产物,但二者有3点完全不同:大白菜Pol CMS不育系扩增产物为675bp,CMS96不育系扩增产物为669 bp,二者相差6个核苷酸,后者定名为大白菜CMS96-orf222。大白菜CMS96-orf222与甘蓝型油菜Nap CMS的nad5c基因和Nap-orf222基因同源性均为99%,E值为0.0;大白菜Pol CMS的675 bp序列具有ORF224开放阅读框,没有保守结构域,而大白菜CMS96的669 bp序列具有ORF222开放阅读框和保守结构域YMF19。另外,atp6和orf222混合引物多重PCR扩增产物存在明显多态性:800 bp为大白菜保持系的差异带型;2 300 bp和1 500 bp为大白菜Pol CMS不育系特异带型;200 bp为大白菜Ogu CMS不育系特异带型;690 bp为大白菜CMS96不育系特异带型。该方法仅用一次PCR反应快速地将3种大白菜细胞质雄性不育系和保持系一次性全部区分开,为大白菜分子育种和常规育种更好地相结合提供了简单、快速、准确和重复性好的方法和手段。     

Effect of Antibiotic-containing Extenders on Taylorella equigenitalis Contaminated Semen

Taylorella equigenitalis is a gram-negative coccobacillus and the causative agent of a transmissible venereal disease in horses known as contagious equine metritis. Outbreaks of contagious equine metritis have been documented in various countries since 1977, with the most recent discovery in the United States in December 2008. During disease occurrences, culturing semen samples for T equigenitalis before breeding may help to prevent transmission of this disease; however, little is known about the antimicrobial activity of equine semen extenders against the organism. The purpose of this study was to investigate the infectivity levels of T equigenitalis in three equine semen extenders inoculated with known concentrations of the organism. The semen extenders used for this study included INRA 96, E-Z Mixin BF, and VMDZ. In addition, Timentin was added to INRA 96 at three different concentrations (0.5, 1.0, and 1.5 mg/mL) to investigate possible synergistic effects of antibiotic supplementation of extenders. Results were based on the visual counting of the colonies on chocolate Eugon agar plates. Both INRA 96 (with added Timentin) and VMDZ (as supplied by the manufacturer) significantly reduced the numbers of T equigenitalis isolated from semen extenders as compared with INRA 96 (as supplied by the manufacturer) or the antibiotic free E-Z Mixin BF. Our findings indicate that INRA 96 (with added Timentin) or VMDZ may significantly decrease the growth of T equigenitalis in extended semen; however, it is also important to consider the possible effects of antibiotic supplemented extenders on sperm longevity and fertility in addition to eliminating specific pathogens in semen.     

皖稻96的特征特性及栽培技术

皖稻96是安徽省农科院水稻研究所采用系谱法选育成的优质双季皖粳糯新品种。两年安徽省区域,平均产量7 593.5 kg/hm2,比对照M1148增产7.5%,全生育期128 d,适宜播种期为6月15~25日。     

Effect of temperature on copper toxicity and hematological responses in the neotropical fish Prochilodus scrofa at low and high pH

Copper sulfate has been widely used to control algae and pathogens in fish culture ponds. However, its toxic effects on fish depend not only on its concentration in water but also on water quality. The susceptibility of the neotropical freshwater fish Prochilodus scrofa to copper was evaluated at two temperatures with low and high water pH. Juvenile fish were acclimated at 20 and 30 °C and exposed to copper (static bioassay system) in water with pH 4.5 and 8.0. The 96 h-LC₅₀ were determined at each temperature and pH, as were the hematological parameters. The 96 h-LC₅₀ for copper (98 and 88 μg Cu L^− 1 in water with pH 4.5 and 16 and 14 μg Cu L^− 1 in water with pH 8.0 for fish kept at 20 and 30 °C, respectively) was significantly dissimilar in pH 4.5 and 8.0, but no difference was found between 20 and 30 °C in the same water pH. At 20 °C, regardless of the water pH, the hematocrit (Hct) increased while the red blood cells (RBC) and hemoglobin (Hb) concentration decreased compared to control pH 7.0. Copper exposure in water pH 4.5 and 8.0 causes an increase on the Hct, RBC and Hb concentrations in relation to the controls pH 4.5 and 8.0. At 30 °C, the changes on the blood parameters depended on the water pH and, after copper exposure at low and high pH, the blood changes indicated more complex responses. The changes in hematological parameters of the fish, regardless of the pH and water temperature, indicate ionoregulatory or respiratory disturbances that imply an increase in energy consumption to restore homeostasis instead of other physiological functions such as weight gain and growth.     

玉米杂交种济单八号的品种特性及配套栽培技术

济单八号,原名济丰四号,济单98-1,是济源市农业科学研究所选育,2003年4月通过河南省农作物品种审定委员会审定。该品种表现突出,株型紧凑,抗病抗倒,综合性状优良。     

高产、优质、多抗玉米新杂交种济单94-2选育及推广应用

济单94-2是河南省济源市农业科学研究所于1994年以自育系济533为母本，外引系选京02为父本组配而成的玉米单交种。该品种属中秆大穗中熟品种，1997～2001年在河南省、国家区域试验、生产试验中表现出高产、稳产、优质、多抗、适应性强等特点。一般产量在7500kg／hm^2以上，适宜在黄淮海夏玉米区和西北春玉米区种植。     

优质高产香型杂交水稻新香优96的选育与应用

新香优96是湖北省荆州市种子总公司用优质香型不育系新香A与自育优质恢复系R96配组育成的中熟香型杂交晚稻新组合。该组合具有米质优、产量高、耐肥耐寒、抗倒伏、熟期适宜、适应性广等特点。2002年2月通过湖北省农作物品种审定。1998—2002年在荆州市累计示范种植6000hm^2以上。     

采绒革盖菌胞外菊粉酶产生条件的研究

新分离到一株能产生较高水平菊粉酶的采绒革盖菌（ＣｏｒｉｏｌｕｓｖｅｒｓｉｃｏｌｏｒＺＱＷ－９６）。通过对培养基组成及培养条件的优化研究发现，用菊芋粉抽提液代替菊粉，氮源为玉米浆，添加０．０４％的吐温—８０的发酵培养基，在ｐＨ５．８、２４℃、转速为１５０ｒ／ｍｉｎ，装液量为７５ｍｌ／５００ｍｌ三角瓶条件下发酵６天后，胞外菊粉酶活性达２．２４ｕ／ｍｌ，比原来提高了１．３倍。关于该菌产菊粉酶的研究，国内外均未见报道     

苹果新种质B96-1的选育及其抗性评价初报

苹果新种质B96-1是我们于1996年以金冠作母本、富士花药培养纯系富85-1作父本杂交育成。果实圆柱形,平均单果重200.0g,果皮黄绿色,阳面带红晕,无果锈;果肉黄白色,肉质松脆,汁液多,风味酸甜适度,品质上等;可溶性固形物含量14.60%,果肉硬度7.03kg/cm2;果实贮至翌年3—4月,不皱皮;抗寒、高抗苹果早期落叶病和苹果果实轮纹病。