全文获取类型
收费全文 | 16489篇 |
免费 | 1353篇 |
国内免费 | 1100篇 |
专业分类
林业 | 690篇 |
农学 | 1261篇 |
基础科学 | 172篇 |
637篇 | |
综合类 | 5507篇 |
农作物 | 1304篇 |
水产渔业 | 1099篇 |
畜牧兽医 | 5045篇 |
园艺 | 771篇 |
植物保护 | 2456篇 |
出版年
2024年 | 46篇 |
2023年 | 285篇 |
2022年 | 481篇 |
2021年 | 615篇 |
2020年 | 635篇 |
2019年 | 680篇 |
2018年 | 476篇 |
2017年 | 651篇 |
2016年 | 706篇 |
2015年 | 656篇 |
2014年 | 881篇 |
2013年 | 912篇 |
2012年 | 1093篇 |
2011年 | 1058篇 |
2010年 | 909篇 |
2009年 | 910篇 |
2008年 | 838篇 |
2007年 | 884篇 |
2006年 | 781篇 |
2005年 | 639篇 |
2004年 | 570篇 |
2003年 | 480篇 |
2002年 | 393篇 |
2001年 | 441篇 |
2000年 | 430篇 |
1999年 | 362篇 |
1998年 | 276篇 |
1997年 | 213篇 |
1996年 | 211篇 |
1995年 | 219篇 |
1994年 | 195篇 |
1993年 | 157篇 |
1992年 | 179篇 |
1991年 | 135篇 |
1990年 | 114篇 |
1989年 | 97篇 |
1988年 | 67篇 |
1987年 | 62篇 |
1986年 | 37篇 |
1985年 | 18篇 |
1984年 | 18篇 |
1983年 | 15篇 |
1982年 | 20篇 |
1981年 | 11篇 |
1980年 | 22篇 |
1979年 | 21篇 |
1978年 | 10篇 |
1976年 | 8篇 |
1956年 | 5篇 |
1955年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 78 毫秒
1.
为改善有刺黄龙果在引种地区的生长势,提高其抗逆性,本研究探讨了不同砧木和嫁接方法对有刺黄龙果嫁接育苗的影响,以期筛选到适宜有刺黄龙果的砧木和嫁接方法。结果表明,不同砧木和嫁接方法对有刺黄龙果的成活率、出芽率和新芽长势有显著影响。嫁接30天时,芽接、楔接、套接和平接法的愈合率显著高于插接和靠接法。嫁接45天时,平接、靠接和楔接法的出芽率较高,分别是76.7%、76.7%和66.7%。嫁接苗田间定植2个月时,平接、靠接和楔接法嫁接苗长势较快。其中楔接法嫁接效率高,适合工厂化批量嫁接。白肉品种‘无刺黄龙’和红肉品种‘金都1号’、‘富贵红’是有刺黄龙果嫁接的优良砧木,嫁接2个月时出芽率分别为86.7%、90.0%和86.7%,且新芽高度和宽度均大于其他砧木。综上所述,适宜有刺黄龙果规模化嫁接育苗的嫁接方法是楔接法,砧木是‘金都1号’、‘富贵红’和‘无刺黄龙’。 相似文献
2.
3.
选取泌乳天数、体重、产奶量相近的荷斯坦高产泌乳牛340头,随机分为试验组和对照组两组,每组170头。试验时间共28d,对照组全期饲喂全混合配方日粮(TMR),试验组分四阶段进行,每阶段7d,分别饲喂以10%、20%、30%、40%粉碎大麦粉替代玉米的TMR。结果发现:随着大麦粉替代玉米比例的增加,试验组采食量呈现下降趋势,其中30%、40%替代期采食量极显著低于对照组(P<0.01);与对照组相比,大麦粉替代不同比例玉米对奶牛产奶量影响不一致,20%、30%替代期产奶量增加0.4、0.3kg/d,10%、40%替代期分别下降0.6、0.7kg/d,但差异均不显著(P>0.05);试验组10%、40%替代期乳脂率极显著高于对照组(P<0.01),而20%、30%替代期乳脂率与对照组差异不显著(P>0.05);试验组10%替代期乳蛋白率极显著高于对照组(P<0.01),而30%、40%替代期显著低于对照组(P<0.05),20%替代期乳蛋白率较对照组下降0.012个百分点,但差异不显著(P>0.05)。综上所述,饲粮中用大麦粉替代20%比例的玉米饲喂奶牛,在不影响采食量、乳脂率、乳蛋白率情况下可提升产奶量并降低饲养成本,从而提高经济效益,为最佳替代比例。 相似文献
4.
The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine. 相似文献
5.
6.
Cassiicolin毒素蛋白是橡胶树棒孢霉落叶病菌的主要致病因子。本研究对分离自海南省5个市县的橡胶树棒孢霉落叶病菌进行了鉴定,形态学鉴定结合分子生物学鉴定结果表明,病原菌为多主棒孢。利用Cassiicolin毒素不同亚型编码基因特异性引物和菌饼活体接种法分别鉴定了分离菌株的毒素蛋白类型和致病性,结果显示,分离的35个菌株都含有Cas5基因,未发现含其他类型的毒素基因,表明海南橡胶树棒孢霉落叶病菌为含Cas5毒素蛋白优势群体;所有菌株对橡胶树品种‘RRIM600’都能够致病,但是在不同抗性品种上致病性存在一定差异。 相似文献
7.
针叶树是芬兰、瑞典、挪威、美国、加拿大等欧美国家主要的商品林树种。容器苗是针叶树重要的育苗类型。与裸根苗比较,容器苗具有育苗周期更短、造林季节可延长、苗木适应性更广、苗木质量更一致的优点。因此,容器育苗成为精准林业发展的必然趋势。为了借鉴欧美容器育苗的技术方法,提高我国容器苗的质量,为我国针叶树容器苗发展提供参考,文中主要从4个方面综述了欧美国家检测与提高苗木质量的方法,即列举了针叶树常用的苗木质量检测指标,总结了育苗容器与育苗基质的改良进展,介绍了养分、水分、光照与温度等培养条件的优化手段,简要概述了容器苗病虫害防控的进展,提出了我国相关树种容器苗生产的启示与借鉴。 相似文献
8.
为研究大麦籽粒支链淀粉、直链淀粉和β-葡聚糖积累特性,及淀粉各组分与β-葡聚糖的关系,以2个皮大麦、1个裸大麦和1个糯裸大麦为试验材料,测定花后7、14、21、28d淀粉各组分及β-葡聚糖含量。结果表明,甘啤6号淀粉各组分含量随灌浆推进均逐渐升高,在成熟期达到最大值;甘垦啤7号和甘垦6号均呈先升高后降低趋势,在花后21d有1个峰值。糯大麦C 2-1直链淀粉含量显著低于非糯大麦;支链淀粉含量显著高于非糯大麦,整个灌浆期呈先升高后降低趋势。β-葡聚糖含量均随灌浆时间延长逐渐升高,成熟期含量最高;整个灌浆期糯大麦C 2-1含量显著高于非糯大麦。相关分析表明,直链淀粉/支链淀粉比值与β-葡聚糖含量呈极显著负相关,可以将直链淀粉/支链淀粉比值作为高β-葡聚糖品种选育的一个指标。Logistic方程拟合发现,直链淀粉、β-葡聚糖最终积累量与积累起势与有效积累时间有关,支链淀粉最终积累量取决于最高积累速率和平均积累速率。 相似文献
9.
Vunjia TIONG Shih-Keng LOONG Haryanti Azura MOHAMAD WALI Kim-Kee TAN Pui-Fong JEE Fang-Shiang LIM Jing-Jing KHOO Pooi-Fong WONG Li-Yen CHANG Sazaly ABUBAKAR 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):280
Corneal lesions appearing as white mass beneath intact epithelium, with ocular discharge in one mouse, was observed in a batch of laboratory-raised BALB/c mice (n=9 of 56). The affected mice remained active, well-groomed and had normal appetite. Isolates recovered from swab cultures of the external and internal contents of the eye had partial 16S rRNA gene sequence of 99.1% similarity to Streptococcus cuniculi. No previous report of S. cuniculi infection in laboratory rodents has been presented. The isolate was susceptible to all antibiotics tested. We suggest S. cuniculi is an opportunistic bacteria in laboratory mice but are uncertain of its source. Our findings revealed that S. cuniculi is able to colonize laboratory mice and should be considered when mice present with eye lesion or ocular discharge. 相似文献
10.
M. A. Macedo A. K. Inoue-Nagata T. N. Z. Silva D. M. S. Freitas J. A. M. Rezende J. C. Barbosa M. Michereff-Filho A. R. Nascimento A. L. Lourenção A. Bergamin Filho 《Plant pathology》2019,68(1):72-84
Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies. 相似文献