全文获取类型
收费全文 | 92474篇 |
免费 | 8781篇 |
国内免费 | 6680篇 |
专业分类
林业 | 8869篇 |
农学 | 10542篇 |
基础科学 | 2447篇 |
10606篇 | |
综合类 | 40076篇 |
农作物 | 9231篇 |
水产渔业 | 4618篇 |
畜牧兽医 | 10986篇 |
园艺 | 4879篇 |
植物保护 | 5681篇 |
出版年
2024年 | 122篇 |
2023年 | 1442篇 |
2022年 | 2714篇 |
2021年 | 3215篇 |
2020年 | 3429篇 |
2019年 | 2800篇 |
2018年 | 2509篇 |
2017年 | 4057篇 |
2016年 | 4943篇 |
2015年 | 4363篇 |
2014年 | 4800篇 |
2013年 | 6007篇 |
2012年 | 7819篇 |
2011年 | 7123篇 |
2010年 | 5685篇 |
2009年 | 5460篇 |
2008年 | 5293篇 |
2007年 | 5854篇 |
2006年 | 5212篇 |
2005年 | 4026篇 |
2004年 | 3149篇 |
2003年 | 2392篇 |
2002年 | 1889篇 |
2001年 | 1762篇 |
2000年 | 1612篇 |
1999年 | 1380篇 |
1998年 | 1169篇 |
1997年 | 985篇 |
1996年 | 884篇 |
1995年 | 911篇 |
1994年 | 767篇 |
1993年 | 792篇 |
1992年 | 708篇 |
1991年 | 612篇 |
1990年 | 565篇 |
1989年 | 396篇 |
1988年 | 301篇 |
1987年 | 221篇 |
1986年 | 168篇 |
1985年 | 91篇 |
1984年 | 56篇 |
1983年 | 27篇 |
1982年 | 32篇 |
1981年 | 38篇 |
1980年 | 37篇 |
1979年 | 26篇 |
1978年 | 22篇 |
1976年 | 12篇 |
1962年 | 13篇 |
1955年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
数字果树及其技术体系研究进展 总被引:2,自引:0,他引:2
果树是重要的农林植物。在万物智联时代,利用数字化、智能化、网络化技术建立数字果树技术体系,实现对果树生命、生产和生态复杂系统的高效感知、认知和智慧管控,对于果业实现数字化转型具有重要意义。该研究在数字植物技术范畴下,系统论述了数字果树的概念和内涵,提出了数字果树技术体系框架。重点在果树表型信息获取、环境数据获取、三维模型计算、数字育种、果树大数据和虚拟现实技术等方面综述了数字果树研究进展。从产业应用角度,综述了数字果树技术在树形管理、生长监测、种植管理、农技培训、品牌营销等方面应用效果和挑战。最后,展望了数字果树发展趋势、研究热点和技术突破方向,以期为数字果树的进一步发展提供思路与借鉴。 相似文献
2.
木薯是热带和亚热带地区重要的粮食和经济作物。木薯种茎是目前唯一的繁殖方式,然而采收后木薯种茎如何应对脱水胁迫及糖代谢基因的表达尚不清楚。本研究通过检测对照组和保水处理组的木薯种茎在采后不同时间点水分和糖分的含量变化来探究离体条件下木薯种茎内糖类发挥的作用。通过检测糖类代谢相关基因的表达来解析不同糖分间的代谢关系及离体种茎的活性状态。结果表明,在保水处理组中随着采后时间的延长,种茎中的果糖、葡萄糖和蔗糖的含量先降低后升高,而海藻糖含量变化正好相反。同时,保水处理组的果糖、葡萄糖和蔗糖的含量显著高于对照组,而海藻糖含量显著低于对照组。qPCR分析发现,严重的脱水胁迫显著地提高了糖酵解基因的表达。这些结论表明,脱水胁迫显著影响木薯茎中糖类的相互转化。推测脱水胁迫前20 d通过合成海藻糖来响应脱水胁迫,在20~30 d则通过分解海藻糖为木薯种茎提供必要的能量物质。同时本研究通过甘油处理提高了木薯种茎的贮藏时间,为木薯种茎的贮藏提供新的方法。 相似文献
3.
为进一步研究国产人工打洞法所结沉香中的化学成分,本研究采用硅胶柱色谱、Sephadex LH-20、半制备高效液相等色谱方法从其乙酸乙酯萃取物中分离纯化得到2个单体化合物。根据核磁共振(NMR)、质谱(MS)等波谱数据分别鉴定为2-(2-苯乙基)色酮二聚体(+)-3′,3′′′-dihydroxy-4′,4′′′-dimethoxyaquisinenone G (1)和aquilasinenone F (2),其中化合物1为新化合物。抗氧化活性测试结果表明,化合物1和2都对DPPH自由基具有一定的清除能力,其中化合物2的IC50 值为(64.6±1.6)mol/L,阳性对照为L(+)-抗坏血酸。 相似文献
4.
5.
The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza⁃tion. Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health. Nowadays, the common detection methods are instrument-based for carbamate pesticide residue, which dis⁃ satisfy the request of an on-site rapid, sensitive and simultaneous detection for multiple carbamate pesticide resi⁃ dues. Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity, a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min. Under the optimized conditions, the limits of detection(LODs)were found to be 0.11 and 0.09 ng/g, while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively. By using spiked peanut samples, it recorded recoveries of 88.4%-98.3%, 90.1%-118.8% for carbofuran and carbaryl respectively. The RSD of intraarray were found at 9.0%, 3.5%, while the RSD of inter-array were recorded at 12.3%, 16.3% respectively. The de⁃
tection results of the microarray were highly consistent with that of HPLC-MS/MS. This proposed microarray provid⁃ ed an on-site high-sensitivity, simultaneous and rapid detection of carbofuran and carbaryl, and could be widely used in the fields of agricultural product quality safety, food safety and environmental monitoring. 相似文献
6.
油菜黑胫病是由Leptosphaeria biglobosa引起的一种真菌病害。这种病害在我国油菜产区广泛发生,并造成一定的经济损失。为通过获取突变体来研究L. biglobosa的生态适应性机制及致病机制,本文优化了影响农杆菌介导转化(ATMT)油菜黑胫病菌L. biglobosa菌株Lb731的因素,评估转化子质量,并筛选相关突变体。结果明确了农杆菌介导转化菌株Lb731的最佳因素:潮霉素B浓度为50 μg/mL,转化受体(分生孢子)培养时间为15 d (20℃),浓度为2 × 107~8孢子/mL,农杆菌-受体共培养温度为25℃,共培养时间为72 h。在最适条件下的转化效率达到80个转化子/百万分生孢子。T-DNA插入基因组的频率为100%,单拷贝插入频率为72.7%,转化子抗潮霉素性状能稳定遗传。从2136个转化子中获得了11个菌丝生长减缓突变体,7个色素产生缺陷突变体和14个分生孢子产生缺陷突变体,并从这些突变体中鉴定出7个致病力丧失突变体。采用hiTAIL-PCR技术,从3个突变体中获得了T-DNA插入位点侧翼序列。上述结果为深入研究L. biglobosa的生态适应性机制及致病机制提供了材料和线索。 相似文献
7.
背主动脉注射是生产转基因鸡的经典方法,该方法不需换壳培养,但壳内注射技术难度大,并且无法实时观察后期胚胎发育情况。本实验对背主动脉注射法进行了改进,将增强型绿色荧光蛋白(EGFP)腺相关病毒(Adeno-associated Virus,AAV)壳外注射至150枚HH 14~16期(Hamburger-Hamilton Stage 14~16)鸡胚背主动脉中,再进行换壳培养,继续孵化至出壳,观察和分析改良背主动脉注射法与传统背主动脉注射法和胚盘下腔注射法对发育8、14、18、21 d鸡胚存活率、孵化率以及EGFP阳性检出率的影响。结果表明:改良背主动脉注射组鸡胚存活率均高于传统背主动脉注射组与胚盘下腔注射组(P<0.05或P<0.01);改良背主动脉注射组的鸡胚孵化率(37%)高于传统背主动脉注射组(16%)与胚盘下腔注射组(28%)(P<0.01);荧光蛋白手电筒检测显示,改良背主动脉注射组鸡胚EGFP阳性率(17%)明显高于传统背主动脉注射组(13%)与胚盘下腔注射组(12%)(P<0.01)。综上,背主动脉壳外注射结合换壳培养提高了转基因鸡胚胎孵化率和EGFP阳性检出率,对提高转基因鸡效率具有重要价值。 相似文献
8.
Synergistic effect of Si and K in improving the growth,ion distribution and partitioning of Lolium perenne L.under saline-alkali stress 下载免费PDF全文
The application of Si or K has proven to be beneficial for the growth of plants under saline-alkali stress. However, the synergistic effect of Si and K in improving the growth, ion distribution, and partitioning in Lolium perenne L. under saline-alkali stress remains unclear. In this study, the growth characteristics and ion-selective absorption of ryegrass(Lolium perenne L.) exposed to different levels of saline-alkali stress were evaluated. The growth parameters of ryegrass were significantly improved when Si was applied by itself or coupled with K under low saline-alkali stress. Under a high saline-alkali level, only simultaneous application of Si and K could significantly improve the growth of ryegrass. When Si and K were applied together, the K~+/Na~+ and Ca~(2+)/Na~+ ratios in root, stem, and leaf of ryegrass were maximally improved as compared to the individual treatments and control. The K~+ and Ca~(2+) concentrations in the vacuole, cell wall, and organelle of leaf were increased dramatically. This improvement was due to the ability of applied ions to compete with Na+, allowing the plant to maintain osmotic potential and leaf water content. The concentration of Na~+ was significantly reduced when Si and K were applied and mainly concentrated in the soluble fraction and cell wall. The Si concentration in ryegrass increased markedly by the combined application of Si and K, and most of it was accumulated in the cell wall and soluble fraction, which could help in chlorophyll synthesis, reduce membrane injury, and increase water absorption under saline-alkali stress. This study emphasized the advantage of Si and/or K on the growth of plants under different saline-alkaline levels and provided a guide for the production of Si-K fertilizer and its application in saline-alkali soil. 相似文献
9.
The priority of management factors for reducing the yield gap of summer maize in the north of Huang-Huai-Hai region,China 下载免费PDF全文
Yue-e LIU Yu-xin LI Tian-fang LÜ Jin-feng XING Tian-jun XU Wan-tao CAI Yong ZHANG Jiu-ran ZHAO Rong-huan WANG 《农业科学学报》2021,20(2):450-459
Understanding yield potential, yield gap and the priority of management factors for reducing the yield gap in current intensive maize production is essential for meeting future food demand with the limited resources. In this study, we conducted field experiments using different planting modes, which were basic productivity(CK), farmer practice(FP), high yield and high efficiency(HH), and super high yield(SH), to estimate the yield gap. Different factorial experiments(fertilizer, planting density, hybrids, and irrigation) were also conducted to evaluate the priority of individual management factors for reducing the yield gap between the different planting modes. We found significant differences between the maize yields of different planting modes. The treatments of CK, FP, HH, and SH achieved 54.26, 58.76, 65.77, and 71.99% of the yield potential, respectively. The yield gaps between three pairs: CK and FP, FP and HH, and HH and SH, were 0.76, 1.23 and 0.85 t ha~(–1), respectively. By further analyzing the priority of management factors for reducing the yield gap between FP and HH, as well as HH and SH, we found that the priorities of the management factors(contribution rates) were plant density(13.29%)fertilizer(11.95%)hybrids(8.19%)irrigation(4%) for FP to HH, and hybrids(8.94%)plant density(4.84%)fertilizer(1.91%) for HH to SH. Therefore, increasing the planting density of FP was the key factor for decreasing the yield gap between FP and HH, while choosing hybrids with density and lodging tolerance was the key factor for decreasing the yield gap between HH and SH. 相似文献
10.