Characteristics and Functional Properties of Ovary from Squid Loligo Formosana

The characteristics and functional properties of the ovary from Loligo formosana were studied. Moisture (72.07 ± 0.24%) was dominant, followed by protein (18.64 ± 0.51%) and carbohydrate (7.44 ± 0.2%). Ash (1.39 ± 0.03%) and lipids (0.46 ± 0.5%) were found as the minor constituents. Albumin (79.02 ± 0.79%) was the major protein of the squid ovary, followed by glutelin-1 (8.31 ± 0.62%) and globulin (6.68 ± 0.08%). Nevertheless, prolamin and glutelin-2 constituted approximately 1% of the total proteins. Based on the electrophoretic studies, albumin had the largest band intensity. The squid ovary was rich in non-essential amino acids (52.26%) and high in hydrophobic amino acids (48.03%). It was also rich in polyunsaturated fatty acid (PUFA, 43.76 ± 0.84%), followed by saturated fatty acid (SFA, 39.36 ± 0.12%) and monounsaturated fatty acid (MUFA, 12.94 ± 0.55%). Ovary lipids had a high amount of docosahexaenoic acid (C22:6) (28.59%). At pH 3, the squid ovary powder (SOP) had the maximum solubility (96.39%), whereas the lowest solubility (38.33%) was observed at pH 9. The foaming capacity and stability of SOP were increased with increasing concentration up to 8% (p < 0.05). The globulin fraction showed the higher foaming capacity, as compared to albumin and glutelin-1 fractions. The squid ovary had good nutritional value and possessed excellent foaming properties. Therefore, the squid ovary could serve as a novel food additive or ingredient.     

Physicochemical and Structural Properties of Recovered Elastin from Jumbo Squid (Dosidicus gigas) By-Products

Physicochemical and structural properties of soluble jumbo squid (Dosidicus gigas) elastin recovered from skin by-products were evaluated. The molecular weight of isolated elastin was ~40 kDa with an isoelectric point (pI) between 9 and 10. Aspartic, glutamic, arginine, proline, glycine, and lysine amino acids were the most abundant in squid elastin, whereas the hydroxyproline absence, ~0.7% cysteine content, and the calculated 0.35 isoleucine/leucine ratio were used as purity index. Total and reactive sulfhydryl contents were similar (247.0 ± 5.1 vs 242.0 ± 7.5 μmol mg^?1 of protein, P ≥ 0.05) in purified squid elastin but surprisingly higher than previously reported in other elastins. On the other hand, the secondary structures of squid elastin analyzed by Fourier transform infrared spectroscopy (FTIR) were ~45% β-sheets, ~15% α-helices, ~10% β-turns, and ~30% undefined structures. In addition, squid elastin experienced glass transition at 82.01 ± 0.01ºC, denaturation temperature at 110.45 ± 0.64ºC, and aggregation at 197.5 ± 0.23ºC. In conclusion, the prevalence of charged amino acids and pI of squid elastin can facilitate its solubilization in hydrophilic systems, whereas the secondary structure profile and thermostability are desirable features in proteins used for biopolymer designs such as food biofilms or barrier systems.     

鱿鱼内脏的营养及其开发利用

按照国标方法,对鱿鱼内脏的营养成分进行检测。结果表明,鱿鱼内脏营养丰富,每100 g鱿鱼内脏中含脂肪21.15 g,蛋白质21.24 g,钙51.46 mg,铁609.07μg和磷95.88μg。此外,鱿鱼内脏消化液中还含有18种氨基酸(包括全部人体必需氨基酸),鱿鱼内脏油脂脂肪酸中含有10.58%的EPA和15.23%的DHA,非常值得研究开发与利用。     

Preparation and Characterization of Protein Hydrolysates From Little Loligo Squid (Uroteuthis chinensis)

Research on the little loligo squid (Uroteuthis chinensis) protein hydrolyzed by papain and Flavourzyme and characterization of hydrolysates was carried out in this article. The optimum hydrolysis conditions of papain were obtained by orthogonal experiments, and the influence factors on the degree of hydrolysis such as enzyme/substrate ratio, hydrolysis time, pH, and temperature were investigated. The effect of Flavourzyme on the degree of hydrolysis was also studied. The characteristics of freeze-dried hydrolysates such as yield, moisture, nitrogen recovery, molecular mass distribution, and amino acid composition were determined. The results showed that papain and Flavourzyme could hydrolyze the little loligo squid protein efficiently, and the degree of hydrolysis was up to 25.82% under the optimum hydrolysis conditions. The average molecular mass of hydrolysates obtained with papain and Flavourzyme was 1,246 Da, and the low molecular mass peptides were the main fraction of hydrolysates. The yield, moisture, and nitrogen recovery of freeze-dried hydrolysates was 19.8, 4.4, and 74.2%, respectively. Compared with suggested patterns of amino acid requirements by the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), the hydrolysates of the little loligo squid had high nutritional value, and was a potential nutritious supplement used in various food products.     

世界头足类资源开发现状和中国远洋鱿钓渔业发展概况

本文简述了世界头足类的资源开发与利用现状，还分析了世界八个主要海区的头足类产量及主要种类。西北太平洋的头足类产量一直保持领先，９０年代仍保持增的势头。１９９５年占世界头足类产量的３５．３％。     

不同处理条件下柔鱼胴体特性的变化

以北太平洋的柔鱼为原料，研究柔鱼的胴体在2℃、50℃和100℃的处理条件下，重量、水分含量及总氮量的变化。通过研究发现，去皮柔鱼胴体在2℃冷水中漂洗时，水分含量的变化是引起重量变化的主要原因，而在漂洗过程中总氮量变化不明显；带皮柔鱼胴体在50℃热水中漂洗时，漂洗水量的增加比时间的延长更有利于防止营养成分的损失；100℃漂烫时，为防止脱水及营养成分的流失，应控制在4分钟内完成。     

不同工艺方法对鱿鱼热风干燥效果的影响

采用热风干燥技术对鱿鱼胴体进行了2组不同工艺方法的试验研究,对2种试验结果进行了对比分析。结果表明,不同干燥工艺方法对鱿鱼干燥效果产生显著影响。采用第2组试验方案进行干燥,可以明显改善干燥中存在的干燥速度不一致、干燥不均匀问题;与第1组试验方案相比缩短干燥时间约2 h,制品的质量也明显提高。     

Interannual variability in stock abundance of the neon flying squid, Ommastrephes bartramii, in the North Pacific Ocean during 1979–1998: impact of driftnet fishing and oceanographic conditions

Variability in catch-per-unit-effort (CPUE) was examined for the autumn cohort of Ommastrephes bartramii collected with research driftnets during 1979–1998 along five longitudinal transects passing through the Subarctic, Transitional and Subtropical Domains in the North Pacific. CPUE was generally low during the period of intensive commercial driftnet fishing (1980–1992) and increased following the 1992 moratorium on the use of large-scale driftnets. However, CPUE levels were low for the cohorts hatched in 1992 and 1996 (captured in subsequent years owing to a one-year life of O. bartramii ) that experienced low sea surface temperatures from hatching to recruitment. Among similar-aged squid collected from 180° and 179°30'W in June, mantle lengths were significantly greater in 1997 than during 1995–96. These findings suggest that the driftnet fishery and sea surface temperatures from hatching to recruitment strongly affected stock abundance and possibly growth.