输卵管炎种鸭大肠杆菌的分离鉴定及生物学特性分析

为分析表现输卵管炎种鸭体内分离的大肠杆菌的致病性、药敏试验、血清型及毒力基因携带情况,无菌采集表现输卵管炎的种鸭肝脏,进行大肠杆菌的分离鉴定,经易感鸭皮下注射,测定其致病性;纸片法进行药敏试验;凝集试验确定血清型;PCR法检测毒力基因.结果共分离鉴定大肠杆菌16株,其中强毒菌株11株.70％以上菌株表现敏感的药物有丁胺卡那霉素、氟苯尼考、头孢唑啉、左氧氟沙星和壮观霉素,70％以上菌株表现耐受的药物有复方新诺明、卡那霉素、庆大霉素、恩诺沙星和氟哌酸.16株大肠杆菌分属于4个血清型,078占56.3％,为主要血清型.毒力基因irp2、yuA、imC和pa pC的检出率分别为68.8％、87.5％、75％和100％.总结说明,从表现输卵管炎的种鸭体内共分离到16株大肠杆菌,078是主要的血清型,首选壮观霉素和氟苯尼考等敏感药物进行治疗.     

一例种鸭输卵管炎大肠杆菌的分离鉴定及药敏试验

对发病种鸭的肝脏、心脏、输卵管内容物进行分离培养、形态镜检和生化试验,在输卵管内容物中分离到1株大肠杆菌。经过药敏试验,结果显示分离菌株对头孢类和氨基糖苷类药物高度敏感,特别是对头孢曲松、庆大霉素高度敏感;对羧苄青霉素、阿莫西林、四环素、强力霉素、多黏菌素B、复方新诺明中度敏感;对喹诺酮类药物、利福平不敏感。     

刘丽教授论治气滞血瘀型输卵管炎不孕症经验

刘丽教授认为输卵管炎型不孕症以气滞血瘀证型多见，临床采用疏肝理气行滞、活血化瘀清热法疏通输卵管，方用加减膈下逐瘀汤，形成疏通输卵管、周期疗法调经、情志疗法助孕三联治疗思路。并附验案2则介绍刘教授临证经验。     

45株鸡卡氏杆菌的分离鉴定

通过形态学检查、鉴别培养、Vitek-32全自动细菌鉴定系统、PCR鉴定和序列分析等方法,对101只鸡的10种组织脏器进行细菌分离和鉴定。最终鉴定出45株鸡卡氏杆菌,其中43株分离自输卵管炎产蛋病鸡,2株分离自具有呼吸道病史的产蛋鸡,而从SPF鸡、公鸡及马立克病患鸡体内未能分出卡氏杆菌;分离的卡氏杆菌对庆大霉素、链霉素、氨苄西林、青霉素、强力霉素、诺氟沙星及磺胺类药物等都具有不同程度的耐药性。试验结果表明,卡氏杆菌与产蛋鸡的输卵管炎具有相关性,鸡卡氏杆菌的抗生素耐药现象较普遍,为预防该病原在我国的流行提供了依据。     

ABDOMINAL MASS DUE TO CHRONIC SALPINGITIS IN AN AFRICAN GREY PARROT (PSITTACUS ERITHACUS)

An adult female African Grey Parrot ( Psittacus erithacus ) was referred with a two year history of intermittent illness and a recently palpated abdominal mass. Hematologic and microbiologic laboratory results on presentation were non-diagnostic. In survey and barium sulfate contrast radiographs there was a large soft tissue mass in the abdominal cavity displacing the proventriculus craniodorsally, and the ventriculus and small intestines cranially. Euthanasia was elected after exploratory laparotomy revealed extensive peritonitis and intestinal adhesions in the coelomic cavity. At necropsy, a 3.5 cm diameter mass was removed from the left oviduct. The histopathologic diagnosis was chronic salpingitis with intraluminal mass, and serositis of many abdominal organs. The oviduct mass consisted of concentric layers of egg yolk and inflammatory cell debris.