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排序方式: 共有24条查询结果,搜索用时 31 毫秒
1.
为了了解高温和利巴韦林处理对马铃薯(Solanum tuberosum L.)卷叶病毒(Potato leave-roll virus,PLRV)和马铃薯Y病毒(Potato Y virus,PYV)脱毒的影响,以宝塔百利、红王和"803"为材料,采用高温和化学处理结合茎尖培养技术对上述马铃薯进行处理,采用RT-PCR方法检测病毒基因。结果表明,长时间的高温处理抑制马铃薯组培苗的生长,"803"的存活率最高为80.1%,宝塔百利的存活率最低为41.1%;利巴韦林对马铃薯生长的抑制作用较明显,其浓度超过75 mg/L时生根效果差,到125 mg/L时对马铃薯生长抑制效果最明显;RT-PCR结果显示,高温处理结合利巴韦林的处理上均未检测到病毒。  相似文献   
2.
Liu YQ  Zhang J  Feng G  Li LH  Yang L  Kou L 《Pest management science》2012,68(10):1400-1404
BACKGROUND: The triazole-based antiviral nucleoside ribavirin exhibited significant in vitro insecticidal effects against Spodoptera litura, a finding that has prompted further investigations into its in vivo insecticidal properties and mode of action against this insect pest. RESULTS: Ribavirin showed delayed insecticidal effects on third-instar larvae of S. litura at a concentration of 1 mg mL−1. When incorporated into an artificial diet, ribavirin caused various effects, depending on the concentration used. Compared with controls, food intake was lower, larval growth was reduced and larval development prolonged. Pupae weight was reduced, and adult morphology was also affected. Relative to the untreated group, treated groups showed higher mortality at larval and pupal stages, which was generally caused by moult disruption. Results of this study with ribavirin resemble those found in treatment with insect growth regulators. CONCLUSION: This work shows that ribavirin merits further study as a lead compound in a novel class of potential insect control agents or for managing field populations of Lepidoptera pests on cruciferous crops. Copyright © 2012 Society of Chemical Industry  相似文献   
3.
对中兽药散剂中利巴韦林含量的高效液相检测方法的不确定度进行评估,分析了各种可能引起利巴韦林测定不确定度的因素,包括标准溶液、称样、体积、稀释量具、检测仪器、色谱峰面积、重复性等进行分析,并通过计算各不确定度分量及合成标准不确定度,得出了液相色谱法测定中兽药散剂中利巴韦林含量的不确定度结果。结果显示影响较大的因素为峰面积测量、标准溶液浓度和重复性。当利巴韦林含量为0.973 mg/g时,其扩展不确定度为±0.017 mg/g。  相似文献   
4.
设计和合成了病毒唑的光探针:5-叠氮基-1-1-(β-呋喃核糖基)-1,2,4-三唑-3-酰胺(1)和3-叠氨-1-(β-呋喃核糖基)-1,2,4-三唑-5-酰胺(2),并以光探针1的前体化合物5-叠氮基-1-(2,3,5-三乙酰基-β-呋喃核糖基)-1,2,4-三唑-3-酰甲酯(3)为模型化合物,初步研究了叠氮基-1,2,4-三唑的光化学性质。结果表明:在不同溶剂(环戊烷和甲醇)中,化合物3在光辐射下得到不同光化学产物,其光化学反应速度快,主要产物明显,且对碳-氢键的插入能力要比插入氧-氢键的强。  相似文献   
5.
复方三氮唑核苷注射液对大白鼠的亚慢性毒性试验   总被引:1,自引:1,他引:0  
将复方三氮唑核苷主射液按高,中,低剂量分别给大白鼠皮下注射,每天1次,连续注射30d,观察试验鼠的生理状态及活动情况测定增重及血液生理生化指标,并进行病理解剖及病理组织学检查。结果表明,复方三氮唑核苷注射液对大白鼠的生理活动血液生理生化指标均无不良影响;病理组织学检查,仅高剂量组有10%大白鼠发生肾小管上皮细胞轻度颗粒变性,其它组各器官均未见异常。  相似文献   
6.
复方三氮唑核苷注射液对鸡新城疫病毒的抑制试验   总被引:2,自引:0,他引:2  
将复方三氮唑核苷(毒菌杀平)注射液、三氮唑核苷液和盐酸吗啉双胍液分别稀释成2,10,20,100μl/L的溶液,分别与等量鸡新城疫病毒(NDV)液混合,其中含抗病毒药含量分别为0.025,0.125,0.250,1.250μg/mL。将混合液37℃感作2h,尿囊腔接种9-11日龄鸡胚,每组接种11个鸡胚,每胚0.1mL,37℃培养,每6-8h照蛋1次,死亡鸡胚及时取出冷冻,活胚孵化至96h全部取出,冷冻后测定血凝效价,计算病毒滴度和病毒灭活率。试验结果表明:含量为0.025,0.125,0.250,1.250μg/mL的毒菌杀平注射液、三氮唑核苷液、盐酸吗啉双胍液对NDV均有不同程度的抑制作用,复方制剂没有降低原抗病毒药的药效,三氮唑核苷对该NDV的抑制效果好于盐酸吗啉双胍。  相似文献   
7.
Summary Potato virus S was eliminated from systemically infected stem cuttings of five potato cultivars after three passages in nutrient media containing 0.003% of the synthetic riboside ribavirin (Virazole). Treatment effects could be detected after only two passages which also revealed differences in cultivar response. Treated plants transferred to ribavirin-free culture media and to soil remained PVS-free. This simple method is less time-consuming than the usual meristem (axillary) tip culture procedures combined with chemotherapy or heat treatment.  相似文献   
8.
The experiment was aimed to establish a drug metabolic model of the blood cells in chicken. The effects of three kinds of culture medium (L-15,M199 and RPMI1640) and different concentrations of fetal bovine serum (FBS) were compared to optimize the culture condition of blood cells,and the proper time of medication was determined after the cell vitality was measured by MTT assay. The content of ribavirin and its metabolites (TCONH2 and RTCOOH) were detected after the ribavirin was dosed. The results showed that the cell viability was highest when the blood cells cultured in L-15 medium,the state of blood cells was better when 10% FBS was added to the medium. The best time for medication was when blood cells were cultured for 3 h. The content of ribavirin was decreased with the time of administration,the metabolites of ribavirin were increased quickly after half an hour, it changed slowly after 3 h. In conclusion,the metabolic model of blood cells in chicken was successfully established,and the blood cells cultured in vitro were better using L-15 medium supplemented with 10% FBS. The metabolic transformation function of blood cells in chicken was indicated by the medication test of ribavirin and it could be used to study the drug metabolism in vitro.  相似文献   
9.
百合病毒脱除技术研究   总被引:2,自引:0,他引:2  
为了探究生产脱毒百合种球的最佳途径,通过对5种百合进行茎尖切块大小、热处理方式以及病毒唑浓度三方面的试验,探茎尖培养、茎尖培养结合热处理、茎尖培养结合病毒唑、茎尖培养加热处理结合病毒唑4种方法对LVX,CMV,LSV 3种病毒的脱除效果。结果显示:综合考虑存活率和脱毒率,单纯的茎尖培养难以脱除病毒,3种方法结合脱毒的效果最好。即茎尖培养(0.5~0.8mm)加热处理(变温热处理)结合病毒唑(10mg/L)的方法脱除病毒的效果最好。  相似文献   
10.
Junin virus (JUNV) is the aetiological agent of Argentine haemorrhagic fever. The pathogenesis of the infection is not well understood, no licensed vaccines exist and no specific antiviral therapy is available. Previous studies have demonstrated the ability of ribavirin to delay and reduce JUNV disease and virus burden in guinea pigs without preventing death. Based on available data, we performed three different studies to determine the efficacy of ribavirin against JUNV in the guinea pig model with a focus on survival. Different doses and treatment schedules of ribavirin were tested in a lethal model of JUNV infection. Our results show that prolonged treatment with high doses of ribavirin significantly reduces the mortality in guinea pigs infected with JUNV. These results may be useful in future experimental studies or clinical testing.  相似文献   
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