Factor Analysis and Comprehensive Evaluation of the Fruit Quality of ‘Yangjiaocui’ Melons

【Objective】The aim of this study was to identify the typical indices of fruit quality and high quality materials of ‘Yangjiaocui’ melons to provide reference and basis for melon quality evaluation and breeding. 【Method】Eighteen indices, including economic trait and flesh quality of fruits from 6 cultivars and 26 high-generation inbred lines of ‘Yangjiaocui’ melons were determined. Based on factor analysis, stepwise regression, correlation analysis and two-dimension ordination map, the typical indices of fruit quality and high quality materials were identified. 【Result】 Among the 18 indices, single fruit mass, hardness, vitamin C (V_C) content, titratable acid content, sugar-acid ratio and solid acid ratio showed greater variability, and the variation coefficients were more than 20%. However, the variability of fruit transverse diameter, flesh thickness rate and pH were relatively lower and the variation coefficients were less than 10%. Three common factors were extracted from economic trait indices with 87.71% cumulative variance contribution rate and the explanatory indices were fruit longitudinal diameter, fruit transverse diameter, fruit shape index, fruit cavity longitudinal diameter, fruit cavity transverse diameter and flesh thickness rate. From quality indices, 4 common factors were extracted with 90.03% cumulative variance contribution rate and the explanatory indices were V_C content, hardness, total soluble solids content, total sugar content, titratable acid content, sugar-acid ratio and solid-acid ratio. Based on the comprehensive analysis of these selected 6 economic trait indices and 7 flesh quality indices, 5 common factors were extracted with 87.35% cumulative variance contribution rate. According to the correlation among the indices and the principle of simplicity and ease of use and avoidance of overlapping information, 5 indices, including fruit longitudinal diameter, flesh thickness rate, hardness, total sugar content and titratable acid content, could be used as typical indices to evaluate the fruit quality of ‘Yangjiaocui’ melons. The comprehensive quality score of all materials were ranked depended on scores and variance contribution rate of the above 5 common factor and two-dimension ordination map. Finally, 10 materials were selected as high quality materials. 【Conclusion】 Fruit longitudinal diameter, flesh thickness rate, hardness, total sugar content and titratable acid content could be used as typical indices to evaluate fruit quality of ‘Yangjiaocui’ melons. Inbred lines 25, 24, 22, 21, 8, 1, 17, 18, 20 and 23 were high quality materials.     

基于‘罗氏沼虾’养殖尾水灌溉条件下稻田养分利用效能提升技术研究

为了实现稻田消纳罗氏沼虾养殖尾水过程中水稻稳产及养分利用效率最大化,设置CK（空白对照）、BC（添加生物炭）、TG（添加土壤改良液）、BT（生物炭及土壤改良液分别减半）4个处理,依据养分运移状况及水稻生长指标的比较,筛选适宜材料类型。结果表明,BC、TG及BT的添加对表层土壤(0~10cm)TN累积、土壤(0~25cm)TP累积及养分损失,BC、TG对土壤(0~25cm)TN累积及养分损失均存在正面效应;TG显著促进表层土壤(0~10 cm)NO₃ ^-累积(P<0.05);除BC外,其他材料的养分表聚作用均促使TN、TP向根部运移(P<0.05);BT处理中灌浆期叶绿素含量显著高于TG和CK(P<0.05),材料处理组在第一节间长度均显著低于CK(P<0.05),其余处理间的差异性均未达到显著水平(P>0.05)。各处理的理论和实际产量间的差异性均未达到显著水平(P>0.05)。综上所述,相较于其他材料,有效截留NO₃ ^-为主氮素养分,提高养殖尾水养分利用率,降低淋洗污染风险;显著控制水稻底部节间生长,降低倒伏风险;且其对水稻生长及产量性状并无显著抑制作用,因而将其作为水稻消纳罗氏沼虾养殖尾水中养分利用效率提升材料具有一定应用潜力。     

‘新海16’愈伤组织诱导及白霜的优化

为获得高品质、活力旺盛且具有分化能力的愈伤组织,本研究在已建立的海岛棉(Gossypium barbadense)‘新海16’再生体系基础上,以MSB附加0.1 mg/L 2,4-D及0.1 mg/L KT为基本培养基,采用单因素随机试验研究外植体不同部位、光照及培养基成分等对‘新海16’愈伤组织诱导的影响。结果表明:‘新海16’愈伤诱导的外植体选用下胚轴中上部绿色茎段诱导效果较优;除此之外,愈伤诱导效果最佳的处理为2 000 lx光照强度、90%MSB无机盐成分、3 g/L PVP及1.2~1.4 g/L Gelrite或Phytagel,均能够降低愈伤组织出现白霜现象,对愈伤组织疏松生长有促进作用。本研究通过优化愈伤组织的诱导方法,提高胚性愈伤组织发生率,为‘新海16’高频再生体系提供科学依据。     

Effect of apple cultivars and storage periods on the virulence of Neofabraea spp.

Bull’s eye rot is a typical quiescent postharvest apple disease in major fruit-growing areas. The susceptibility of different apple cultivars to Neofabraea spp. (N. vagabunda and N. malicorticis) was assessed, with Granny Smith showing the most resistance and Cripps Pink the most susceptibility. To assess the factors involved in conidial germination, Neofabraea spp. were grown on crude protein extracts (CPEs) collected from apple fruits at different storage periods. Fungal germ tube growth rate and pathogenic enzyme (cellulase and xylanase) activity were assessed. Results showed that CPEs collected after 2 and 4 months of storage progressively stimulated conidial germination and germ tube elongation, while a lesser effect was observed from CPEs after 1 month of storage. Xylanase proved to be the main degrading enzyme secreted by all the isolates, while cellulase was produced only by N. vagabunda isolates. Overall, the isolate ID02 was the most virulent, based on more rapid germ tube elongation and greater activity of the lytic enzymes.     

高光谱成像快速检测壳聚糖涂膜草莓可溶性固形物

为了对壳聚糖涂膜草莓可溶性固形物含量(soluble solids content, SSC)进行快速检测,该文采用高光谱成像仪(400～1 000 nm)对0,0.5%,1%浓度的壳聚糖(chitosan, CTS)涂膜草莓分别储藏1,2,4 d后进行成像,并测量样本SSC。通过分析SSC发现,0.5%和1%壳聚糖涂膜草莓,其SSC随着储藏天数的增加均高于0浓度壳聚糖涂膜草莓,说明了0.5%和1%壳聚糖涂层抑制了草莓中SSC的降低,能够延长草莓的新鲜口味。随后采用蒙特卡罗-偏最小二乘法(monte carlo-partial least squares, MCPLS)对异常样本进行剔除。对剔除异常样本后的光谱数据进行不同预处理,以确定最优的预处理方法。为提高运行速度和降低数据维数,采用竞争性自适应权重取样法(competitive adaptive reweighted sampling, CARS)和连续投影算法(successive projections algorithm, SPA)进行特征波段选择。最后,采用偏最小二乘回归(partial least square regression, PLSR)和支持向量回归(support vector regression, SVR)法建立回归模型。最终结果表明:SPA-SVR模型效果最佳,0浓度的壳聚糖涂膜的草莓,建模集精度Rc2为0.865,预测集精度Rv2为0.835;0.5%浓度的壳聚糖涂膜的草莓,建模集精度Rc2为0.808,预测集精度Rv2为0.799;1%浓度的壳聚糖涂膜的草莓,建模集精度Rc2为0.834,预测集精度Rv2为0.875。对储藏第4天的部分样本图像进行主成分分析(principal component analysis, PCA),结果显示除第二主成分图像(PC2)中有部分噪声影响外,PC1和PC3均能完整反映草莓信息,且PC3图像明显呈现出不同浓度壳聚糖涂膜草莓的褐变程度,说明不同浓度的壳聚糖涂膜也会对草莓货架期产生不同影响。综上说明利用高光谱成像技术可以实现壳聚糖涂膜草莓SSC快速检测,有效指导草莓保鲜处理。     

Occurrence of prophage and historical perspectives associated with the dissemination of huanglongbing in mainland China

Huanglongbing (HLB), associated with a non-culturable bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), is a highly destructive citrus disease with a long but poorly documented history in China. No effective treatment for HLB is available. The identification of new prophages in abundant CLas genomic sequence data provides new insights into both the diversity of CLas strains and HLB management. In this study, CLas populations from nine provinces were surveyed for the presence of prophage. Two major prophage typing groups (PTGs) were discovered to be associated with two different altitude regions: strains of CLas in PTG1 from high altitude regions (HAR) mainly contained prophage Type 1 only or Types 1 and 3, whereas strains of CLas in PTG2 from low altitude regions (LAR) mainly contained prophage Type 2. The discovery of these CLas population patterns provides evidence for independent origins of HLB in HAR and LAR. Guangdong province is the generally recognized domestic region of origin for HLB and is primarily responsible for the dissemination of HLB in LAR through transport of seedlings. Both Yunnan and Sichuan provinces are the probable regions of origin for HLB in HAR. PTG2 was further divided into two subgroups: PTG2-1, found in Guangdong, Fujian and Guangxi and PTG2-2, found in Jiangxi, Zhejiang and Hunan. These regions and prophage types are correlated with early and late introductions of HLB in LAR. These molecular analyses were supported by studying the history of the dissemination of HLB in historical documents.     

水果番茄新品种“晶杏”的选育

"晶杏"是由XS12为母本、CM14为父本杂交而成,为无限生长型,生长势旺盛,中熟,果实淡黄色,高圆形,单果质量约50 g。色泽亮丽,坐果率高,坐果整齐一致,果实大小均匀。丰产性好,口味佳,抗裂果,高抗叶枯病和枯萎病。产量约达97.5 t·hm^-2,是目前栽培的理想樱桃番茄品种,适合于内蒙古中部地区早秋、早春日光温室和大棚越夏栽培。     

辣椒新品种“鄂椒香帅”的选育

"鄂椒香帅"是以BL8为母本,CA1324-4为父本育成的鲜食长线椒辣椒杂交一代品种。该品种植株生长势较强,株高55 cm,开展度52 cm,叶片绿色,卵圆形,少茸毛,单叶互生,分枝性较强且分枝处有紫色斑块,始花节位8~9节,花冠白色,花萼平展;坐果力较强,果实长28~32 cm,果实横径1.3 cm,果肉厚0.2 cm,果实长羊角形,椒条顺直,果面较光滑,三心室,单果质量约26 g,果色绿色,肉质脆,味辣,适合鲜食。抗疮痂病、中抗黄瓜花叶病毒病(CMV),挂果性好,产量高;适合长江流域地区春夏季露地和高山栽培。     

1-MCP处理对“东红”猕猴桃货架期品质的影响

以"东红"猕猴桃为试材,通过采后用不同浓度(0、0.25、0.50、0.75μL·L^-1)1-甲基环丙烯(1-methylcyclopropene,1-MCP)进行处理后,将果实至于(20.0±0.5)℃层析冷柜中对其进行货架期贮藏,研究"东红"猕猴桃果实品质的变化,以期为延长猕猴桃货架期品质提供参考依据。结果表明:0.75μL·L^-11-MCP能够有效地抑制"东红"猕猴桃果实硬度、b*值、超氧化物歧化酶(SOD)活性及过氧化氢酶(CAT)活性的下降;0.75μL·L^-1及0.50μL·L^-11-MCP均能够显著降低"东红"猕猴桃果实呼吸强度、乙烯生成速率和脂氧合酶(LOX)活性;0.25、0.50、0.75μL·L^-11-MCP能够更好地推迟"东红"猕猴桃果实可溶性固形物含量的上升,而不同浓度1-MCP处理间对"东红"猕猴桃果实可滴定酸含量的作用效果无显著差异。综合比较,0.75μL·L^-11-MCP能够更好地保持"东红"猕猴桃的货架期品质,其次为0.50μL·L^-11-MCP。综合考虑"东红"猕猴桃货架期品质变化及成本,建议"东红"猕猴桃的1-MCP使用浓度为0.50~0.75μL·L^-1。     

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.