自噬调节剂对感染日本脑炎病毒小鼠脑部细胞凋亡的影响

旨在研究自噬调控药物对感染日本脑炎病毒（Japanese encephalitis virus，JEV）小鼠脑部细胞凋亡的影响，本试验建立自噬调控药物处理的感染日本脑炎病毒小鼠的动物模型，其中，雷帕霉素为自噬诱导剂，渥漫青霉素及氯喹为自噬抑制剂。实验动物分成8组：DMEM对照组（Control）；JEV感染组（JEV）；JEV+雷帕霉素（Rapamycin）组（JEV+Rapa）；JEV+渥漫青霉素（Wortmannin）组（JEV+Wort）；JEV+氯喹（Chloroquine）组（JEV+CQ）；雷帕霉素组（Rapa）；渥漫青霉素组（Wort）；氯喹组（CQ）。观察不同处理组小鼠的临床症状；透射电镜观察小鼠脑部神经元及胶质细胞的线粒体损伤程度；Tunel染色观察统计小鼠脑部凋亡细胞分布；检测小鼠脑部凋亡因子及凋亡蛋白的表达量。与JEV+Rapa及JEV组相比较，JEV+Wort及JEV+CQ组小鼠出现轻微的神经症状，脑部神经元及胶质细胞线粒体轻度损伤，脑组织较少细胞发生凋亡。不同处理组小鼠脑部凋亡因子及凋亡蛋白的表达量变化差异不显著。综上表明，自噬抑制剂渥漫青霉素和氯喹可以在一定程度上抑制感染日本脑炎病毒小鼠脑组织中细胞凋亡的发生。     

Effects of five compound attractants in high plant‐based diets on feed intake and growth performance of juvenile turbot (Scophthalmus maximus L.)

An experiment was conducted to evaluate the effects of five attractants on feed intake, growth performance and appetite‐regulating genes expression for juvenile turbot (Scophthalmus maximus L.). Fish were fed with seven diets: fish‐meal‐based diet (FM), fermented soybean meal replacing 45% fish meal (FSBM) and other diets with 1% compound attractant A (CA‐A) consisting of amino acids, compound attractant B (CA‐B) consisting of quaternary ammonium bases, compound attractant C (CA‐C) consisting of nucleotides, compound attractant G (CA‐G) consisting of the combination of CA‐A, CA‐B and CA‐C and blue mussel protein hydrolysate (BMH) on the basis of FSBM diet respectively. Daily feed intake in CA‐A group was significantly higher, but that in CA‐B group was significantly lower than FSBM group. Similarly, specific growth rate was significantly enhanced in CA‐A and CA‐G groups and were significantly decreased in CA‐B group. Protein efficiency ratio and protein retention in CA‐B group were significantly lower than FSBM group. Furthermore, BMH supplement increased the mRNA expression of Ghrelin in the intestine of fish. These results showed that CA‐A and CA‐G supplement could enhance growth in plant‐based diets, but CA‐B supplement had the opposite effect, which might be associated with lower feed intake and lower feed utilization.     

黑龙江省国有林区林业产业结构调整与优化研究

黑龙江省国有林区林业曾为我国经济与社会建设做出过突出贡献,尽管目前该区域已形成了较完整的林业产业体系,但林业产业结构的诸多弊端表现得愈发突出,致使林业产业效益低下.随着环境的变化,黑龙江省国有林区林业产业结构的调整与优化显得越来越必要和迫切.黑龙江省国有林区林业产业结构的调整与优化必须依据相关理论,根据林业产业发展的特点和规律,遵循相关的经济政策进行.同时根据产业结构调整优化的目标,形成科学合理的产业发展模式,并采取有效的措施,达到黑龙江省国有林区产业结构合理化和高度化的目的.     

通用小型汽油机游车故障的一种非常规排除方法

分析了通用小型汽油机游车的原因，提出了一种较为经济的游车故障排除方法。     

渍前和渍后喷施外源调节物质对花期渍水小麦生长和产量减损效应的差异

为明确渍前和渍后施用外源物对长江中下游地区渍水小麦生长和产量减损效应的差异，以当地主栽品种扬辐麦4号为供试材料，对渍前和渍后喷施外源调节物质(DDTC、GA、肥料元素和复配外源物)后花期渍水胁迫下小麦光合与叶绿素荧光特性、干物质积累与转运及籽粒产量进行了比较分析。结果表明，花期渍水胁迫后小麦光合性能减弱，光能利用和电子传递受阻，花后光合同化物积累减少，籽粒灌浆速率降低，粒重和产量下降。渍前和渍后喷施外源调节物质均可提高花期渍水小麦的叶片光合性能，增强叶片光能利用和电子传递能力，促进籽粒中花后光合同化物积累，提升籽粒灌浆速率以及粒重，从而增加籽粒产量。与渍后喷施相比，渍前喷施外源物能够有效提高花期渍水小麦的叶片光合速率、光化学效率和叶绿素含量以及降低热耗散；渍前喷施外源物能够有效促进花期渍水小麦的花后光合同化物积累，降低花前营养器官贮藏物转运；渍前喷施外源物能够有效提升花期渍水小麦的籽粒灌浆速率，促进粒重和籽粒产量增加。综上所述，与渍后喷施相比，渍前喷施外源调节物质能够有效提升花期渍水小麦光合性能，促进干物质积累与转运以及降低籽粒产量损失，从而减少渍水胁迫对小麦生长和产量的不利影响，其中...     

机器视觉技术在温室营养液调控系统中的应用

现代设施农业趋向于采用无土栽培.无土栽培是一种采用灌溉营养液来栽培作物的方法.为此,介绍了国内温室无土栽培中营养液供给系统的研究现状,针对目前营养液供给控制存在的问题,将机器视觉技术应用到温室营养液调控中,利用作物的图像信息和环境信息,参考作物专家系统,提出控制策略,调整营养液配置参数,并阐述了系统的软硬件实现方法.     

Tentative Research About the Macroscopic Regulating to Blast

The paper analyzes the difference of the pathogenic varieties and the relationship of the variety composition,allording to the historic material to the varieties of the main hybid fece varieties in Southeast Sichuan during 1992 to 1998.It's the first time to advance to use the camparison on rate of pathogenic isolates between norm and refrieving to define the susceptible extent of the varity.It's an scientific base to adjust the structures of the vartieties,to reach the objective of the macroscopic regulating to rice blast.     

Simulation Investigation for the Regulating Characteristics of Real and Reactive Power of Asynchronized Turbogenerators

This paper discuas the controlling principle of asynchronized turbogenerators,deduces their mathematical model,and studies their regulating characteristics of real and reactive power(or voltage)by means of simulation calculation of operating characteristics for a 200MW asychronlzed turbogenerator.The conclusion is that the asychronlzed tubogenerators not only can regulate real and reactive power independently but also has an excellent dynamic performance.     

水土保持在综合调控流域水资源配置中的作用

 水是人类社会发展和经济活动的重要战略资源。从总体来看,我国水旱灾害呈现出发生频率逐渐增大、灾情愈渐加重的趋势。水土流失是导致水旱灾害日益严重的重要原因之一。水土保持是水资源调控和优化配置的重要措施,是解决水问题,实现水利事业可持续发展的重要途径。从调控和优化配置水资源的角度出发,如何科学合理地配置水土保持措施,还有必要系统地开展理论和技术研究。     

PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响

为探明东北地区常见野生葱属植物耐旱能力,筛选野生葱属植物耐旱种质资源,本研究以野韭（Allium ramosum L.）、山韭（Allium senescens L.）和蒙古韭（Allium mongolicum Regel）3种葱属植物为材料,采用不同浓度的PEG-6000模拟干旱胁迫,分析其种子萌发期渗透调节物质及酶活性的影响,并结合主成分分析和隶属函数分析比较抗旱性。结果表明：干旱胁迫下,3种葱属植物种子萌发期相对电导率显著升高（P<0.05）,丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量及超氧化物歧化酶、过氧化氢酶、过氧化物酶、多酚氧化酶活性均呈现先上升后下降的趋势。3种植物相比,山韭相对电导率最低,渗透调节物质积累量最高,酶活性变化明显。主成分分析表明,4种酶活性之间显示出强相关性。隶属函数分析得出3种植物种子的抗旱性由高到低依次为：山韭 > 野韭 > 蒙古韭。