全文获取类型
收费全文 | 347篇 |
免费 | 35篇 |
国内免费 | 11篇 |
专业分类
林业 | 32篇 |
农学 | 15篇 |
基础科学 | 36篇 |
31篇 | |
综合类 | 191篇 |
农作物 | 12篇 |
水产渔业 | 12篇 |
畜牧兽医 | 43篇 |
园艺 | 6篇 |
植物保护 | 15篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 15篇 |
2021年 | 18篇 |
2020年 | 15篇 |
2019年 | 8篇 |
2018年 | 12篇 |
2017年 | 6篇 |
2016年 | 16篇 |
2015年 | 16篇 |
2014年 | 23篇 |
2013年 | 16篇 |
2012年 | 29篇 |
2011年 | 17篇 |
2010年 | 18篇 |
2009年 | 29篇 |
2008年 | 20篇 |
2007年 | 27篇 |
2006年 | 15篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 15篇 |
2002年 | 10篇 |
2001年 | 5篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有393条查询结果,搜索用时 15 毫秒
1.
为探究苦参碱灌胃和腹腔注射不同给药途径对肠道菌群的影响,将昆明小鼠随机分为4组,分别为生理盐水腹腔注射组(NCip)、生理盐水灌胃组(NCig)、苦参碱腹腔注射组(MTip)及苦参碱灌胃组(MTig),连续给药5d后,收集各组粪便,进行小鼠肠道微生物α多样性指数分析和β多样性分析,利用LEfSe和Metastats分析不同组间肠道菌群的差异。结果表明:灌胃和腹腔注射苦参碱的不同给药方式对小鼠肠道菌群的α多样性指数及β多样性的影响差异不显著(P0.05);LEfSe分析发现,与灌胃苦参碱相比,腹腔注射苦参碱组的小鼠肠道有益菌拟杆菌目(Bacteroidales)、调节机体代谢的另枝菌属(Alistipes)丰度显著增加(P0.05);Metastats分析发现,与灌胃苦参碱相比,苦参碱腹腔注射组的小鼠肠道与结肠炎相关的脱硫弧菌属(Desulfovibrio)、[Eubacterium]_fissicatena_group丰度显著降低(P0.05)。综上,不同给药途径会引起肠道菌群的差异,相比于灌胃给予苦参碱,通过腹腔注射给予苦参碱更有利于优化肠道菌群的结构及功能。 相似文献
2.
近年,生猪养殖产业不断向着集约化规模化方向发展,养殖密度增加的同时,各种传染性疾病呈现高发趋势。猪传染性疾病发生后,会造成生长发育不良,行动迟缓,生理指标失衡,甚至引发死亡,带来的经济损失不可估量,某些人畜共患病还会威胁人类的身体健康。在疫病防控过程中,需要从传染源、传播途径和易感群体等角度出发,减少养殖环境病原数量,切断病原传播途径,保护易感群体,确保生猪健康生长。该文主要结合一个乡镇的疫病流行特点,分析切断传染源和传播途径对防治猪病发生流行的重要性。 相似文献
3.
果品产业高质量发展是兼顾经营者、生产者、消费者利益的更高层面的发展;推动山东果品产业高质量发展对于应对产业面临的挑战与压力、促进乡村振兴、落实新发展理念、增加农民收入等都具有十分重要的意义;山东果品产业发展水平具备了由追求高产量向追求高质量发展转变的客观条件;推动山东果品产业高质量发展,需要在充分发挥市场配置资源的决定性作用的同时,更好地发挥政府作用。 相似文献
4.
Colonization of Aeromonas salmonicida subsp. masoucida strains in Atlantic salmon (Salmo salar L.) during infection 下载免费PDF全文
Yishuai Du Pengfei Liu Lingjie Meng Zaki Sharawy Ying Liu 《Aquaculture Research》2018,49(5):1826-1833
Aeromonas salmonicida subsp. masoucida (ASM) is classified as atypical A. salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS‐C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salar L.) by an immersion challenge with the control group (T0, no AS‐C4), group T1 (2.67 × 104 CFU/ml AS‐C4) and group T2 (2.67 × 107 CFU/ml AS‐C4). The numbers of AS‐C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real‐time PCR (qRT‐PCR). AS‐C4 were detected in the gill and intestine as early as 0 hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS‐C4 into salmon. Although AS‐C4 could not be detected in the skin until 24 hr after the challenge in T1 group, it could be detected in the skin as early as 0 hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS‐C4 into salmon. AS‐C4 was immediately detected in the blood within 3 hr after it entered the host, suggesting that AS‐C4 successfully invaded the bloodstream of fish. After AS‐C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture. 相似文献
5.
Razan F. Al-Qadri Ehab A. Abu-Basha Mohammed M. Ababneh Zuhair Bani Ismail Nasir M. Idkaidek 《Journal of veterinary pharmacology and therapeutics》2020,43(4):339-346
The objectives of this study were to determine the pharmacokinetics of toltrazuril and its metabolites in pregnant and nonpregnant ewes following a single oral dose and to determine the plasma concentrations of these compounds in milk, allantoic fluid, and newborn plasma. Eighteen healthy ewes were randomly divided into three groups (n = 6 each): pregnant ewes at 12–13 weeks of gestation (group A), nonpregnant ewes (group B), and pregnant ewes at 1–2 weeks before expected lambing date (group C). Ewes in all groups received a single oral dose of toltrazuril at 20 mg/kg body weight. In groups A and B, blood samples were collected at 1, 3, 5, 7, 9, 12, 15, 18 hr, every 6 hr to day 3, every 12 hr to day 7 and thereafter every 24 hr to day 14 post-toltrazuril administration. In group C, parturition was induced 24–36 hr after toltrazuril administration then milk, allantoic fluid, and newborn plasma samples were collected immediately after birth. Drug metabolites were assayed using ultra high-performance liquid chromatography–ultraviolet detection method (UHPLC-UV). The maximum concentration (Cmax), area under the plasma concentration-time curve (AUC0–t), AUC to 24 and 48 hr (AUC0–24), and (AUC0–48) were significantly higher in pregnant ewes. Longer apparent half-life (T1/2), significantly higher apparent volume of distribution (Vd/F) and total clearance (Cl/F) were observed in nonpregnant ewes. The time to maximum plasma concentration (Tmax), mean residence time (MRT) and elimination rate constant (Kel) were similar in both groups. The AUC0–24 and AUC0–48 were significantly higher in nonpregnant ewes. The AUC0–t was significantly higher in pregnant ones. The ratio of plasma toltrazuril concentrations in ewes and toltrazuril concentrations in newborn lambs' plasma, allantoic fluid, and milk were 68%, 2.3%, and 5.3%, respectively. Results of this study showed that toltrazuril is well absorbed after a single oral dose in ewes with widespread distribution in different body tissues. 相似文献
6.
7.
基于WEB的无人机三维仿地飞行规划在农业植保中的应用 总被引:1,自引:0,他引:1
【目的】我国是一个农业大国,农业生产在我国占着举足轻重的地位,无人机植保在农业生产中应用越来越广泛,极大地促进了农业植保的快速发展。建立易操作的智能化平台更有利于无人机植保的快速推广应用。【方法】文章提出了建立基于Web的无人机飞行规划平台,在平台中集成影像和DEM等各类数据,由专业技术人员进行远程三维仿地飞行规划,普通用户只需要在移动飞行规划平台上同步下载规划任务,完成傻瓜式的一键飞行,顺利完成植保任务。【结果】通过实地实验测试,文章中的研究方案可以智能化地完成农业植保任务,显著提高无人机植保的安全性和易用性。【结论】针对现代化农业植保中存在的问题,文章提出的基于WEB的无人机三维仿地飞行规划在农业植保中的应用方案,可以有效解决无人机植保操控复杂、技术程度要求高等问题,提升了农业植保的信息化、智能化程度。 相似文献
8.
9.
10.
2012年,中央“一号文件”提出要“大力培育新型职业农民”。新型职业农民是指以农业为职业、具有一定的专业技能、收入主要来自农业的现代农业从业者。本次调查统计的结果表明安徽省新型职业农民的法律意识不强,知权、权利运用以及依法维权能力严重不足。而造成这一问题的主要原因有:农民文化水平整体较低、法制教育重视不足、小农意识及农村社会环境对农民的影响等。针对以上问题,结合当下安徽以及中国的实际,加强新型职业农民的权利实现能力应当采取以下措施:夯实并完善新型职业农民培训、加强新型职业农民自治组织建设、建设“权利本位”的新法律文化,等。 相似文献