我国主要麦区农户施肥评价及减肥潜力分析

【目的】明确我国主要麦区农户小麦施肥存在的问题及减肥潜力,为科学施肥、合理减肥提供依据。【方法】连续3年对我国主要麦区的小麦种植户进行施肥调研和取样,基于农户产量、养分需求量和土壤养分供应水平对其施肥状况和减肥潜力进行评价和分析。【结果】我国主要麦区农户小麦产量和生物量平均为6.0和13.2 t·hm^-2,二者极显著线性相关。小麦产量与施肥量和土壤养分无显著相关。我国小麦氮(N)、磷(P₂O₅)和钾(K₂O)肥用量平均分别为191.1、112.8和53.4 kg·hm^-2,春麦区农户氮、磷和钾肥用量平均分别为171.7、108.9和10.6 kg·hm^-2,旱作区分别为154.3、111.8和32.6 kg·hm^-2,麦玉区分别为236.4、128.1和74.0 kg·hm^-2,稻麦区分别为177.5、77.0和71.8 kg·hm^-2。就施氮量而言,春麦区过量施氮的农户较少,为34%,其次...     

磷石膏改良剂对土壤水分扩散率的影响

以靖远(A)、瓜州(B)、金昌(C)和新疆(D)4个区域盐渍化土壤为对象,采用水平土柱入渗法,探讨磷石膏盐碱土改良剂不同用量(0、6 000、12 000 kg·hm~(-2))对湿润峰移动平均速率和土壤水分扩散率的影响。结果表明:不同用量磷石膏盐碱土改良剂对4种盐渍化土壤水分扩散率影响不同。对于A、B、D 3个区域盐渍化土壤,改良剂用量为6 000 kg·hm~(-2)以下时,对土壤水分传输潜在能力有促进作用;当改良剂用量超过6 000 kg·hm~(-2)时,改良剂对土壤水分传输潜在能力有抑制作用。而对于C区域盐渍化土壤,随着改良剂用量的增加,对水分传输潜在能力有明显的促进作用,这说明改良剂对C区域盐渍化土壤作用效果明显。     

磷肥对白蜡生长量的影响

以乔木白蜡为试验品种,通过对不同时期土壤和植物叶片取样的养分检测及对植物生长量的监测,探讨不同施肥处理,特别是施用磷肥对白蜡生长量的影响。结果表明:合理使用有机肥和无机肥,特别是磷肥的合理使用,增加了植物对氮肥的吸收,促进了土壤氮素释放。说明了磷肥在白蜡生长过程中所起的重要作用,打破了园林苗木生长过程中主要以氮肥为主要肥料施用的观念,阐述了磷肥在白蜡生长过程中对其生长量所产生的显著影响。     

黄泥田晚稻肥料效应的研究

肥料是作物增产的重要因素。在农田施肥实践中,氮、磷、钾肥最佳用量及合理配比因土壤不同而不同。为此,采用3个因子4、个不同施肥用量水平,展开了黄泥田氮磷钾肥料效应的试验,推荐了最佳施肥用量。     

一株柠条内生解磷菌的分离鉴定及实时荧光定量PCR检测

结合形态观察与16S rDNA序列测定对柠条根系内分离筛选得到的解磷细菌C9进行鉴定,Blast比对结果表明C9为泛菌(Pantoea vagans)。在无机磷液体培养基培养条件下,用钼锑抗比色法研究它的解磷能力。结果表明,随着时间的延长,培养液中速效磷含量逐渐增加到4.45mg/L,溶液pH可降至4.2。进一步利用实时荧光定量 PCR 检测柠条根系中、柠条根际土壤与柠条根围土壤中该细菌存在的相对基因拷贝数,结果发现该基因在3种样品中的数量为:柠条根系>柠条根际土壤>柠条根围土壤,表明泛菌解磷细菌能聚集生长在柠条根系内,随着与根系接触距离的增加而呈逐级递减趋势。     

近代黄河三角洲段渤海海岸线缓冲带土地利用时空特征分析

通过解译1986、1996和2001年遥感图像,获得了黄河三角洲海岸带土地覆被数据。将该地区土地覆被分为9种类型：水域、芦苇、林地、耕地、柽柳、柽柳芦苇、翅碱蓬獐茅、滩涂、建设用地,并将3 km宽的海岸带分为6个缓冲带,每个带宽0.5 km。运用ARC/INFO软件和景观生态学方法,研究了渤海海岸线缓冲带的土地利用和景观格局变化。主要结果如下：在空间上,1986年和2001年各缓冲带土地利用程度综合指数随着距海岸线距离的增大而升高,两者呈直线关系;但在1996年,两者之间未呈现直线关系。在时间上,各缓冲带土地利用程度综合指数逐年增加;除了优势度外,所研究的其他景观格局指标与距海岸线的距离没有显著关系。     

食品添加剂磷酸氢二铵中砷含量的测定

运用原子荧光光谱法对进出口食品添加剂磷酸氢二铵中砷含量进行了测定,通过线性范围、检出限、精密度、回收率和实验室间比对测试,证明了原子荧光光谱法能快速、准确地检测出进出口食品添加剂中砷的含量。该方法线性关系良好,检出限低,准确度高,重复性和再现性好,可广泛用于进出口食品添加剂中砷含量的检测。     

Proton accumulation accelerated by heavy chemical nitrogen fertilization and its long-term impact on acidifying rate in a typical arable soil in the Huang-Huai-Hai Plain

Cropland productivity has been signiifcantly impacted by soil acidiifcation resulted from nitrogen (N) fertilization, especialy as a result of excess ammoniacal N input. With decades’ intensive agricul...     

DMPP mitigates N2O and NO productions by inhibiting ammonia-oxidizing bacteria in an intensified vegetable field under different temperature and moisture regimes

Vegetable soils with high nitrogen input are major sources of nitrous oxide (N₂O) and nitric oxide (NO), and incorporation of the nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) into soils has been documented to effectively reduce emissions. However, the efficiency of DMPP in terms of soil N₂O and NO mitigations varies greatly depending on soil temperature and moisture levels. Thus, further evaluations of DMPP efficiency in diverse environments are required to encourage widespread application. A laboratory incubation study (28 d) was established to investigate the interactive effects of DMPP, temperature (15, 25, and 35 ℃), and soil moisture (55% and 80% of water-holding capacity (WHC)) on net nitrification rate, N₂O and NO productions, and gene abundances of nitrifiers and denitrifiers in an intensive vegetable soil. Results showed that incubating soil with 1% DMPP led to partial inhibition of the net nitrification rate and N₂O and NO productions, and the reduction percentage of N₂O production was higher than that of NO production (69.3% vs. 38.2%) regardless of temperature and soil moisture conditions. The increased temperatures promoted the net nitrification rate but decreased soil N₂O and NO productions. Soil moisture influenced NO production more than N₂O production, decreasing with the increased moisture level (80%). The inhibitory effect of DMPP on cumulative N₂O and NO productions decreased with increased temperatures at 55% WHC. Conversely, the inhibitory effect of DMPP on cumulative N₂O production increased with increased temperatures at 80% WHC. Based on the correlation analyses and automatic linear modeling, the mitigation of both N₂O and NO productions from the soil induced by DMPP was attributed to the decreases in ammonia-oxidizing bacteria (AOB) amoA gene abundance and NO^-₂-N concentration. Overall, our study indicated that DMPP reduced both N₂O and NO productions by regulating the associated AOB amoA gene abundance and NO^-₂-N concentration. These findings improve our insights regarding the implications of DMPP for N₂O and NO mitigations in vegetable soils under various climate scenarios.     

一株分离自葛藤根际高效溶磷细菌特性研究及菌株鉴定

对分离自葛藤(Pueraria lobata L.)根际的一株高效溶磷细菌GTR15进行促生特性、主要生理生化指标测定和16S rDNA序列分析。结果表明,菌株GTR15的HD/CD值(溶磷圈直径HD,菌落直径CD)为2.22,28℃液体振荡培养7 d后对磷酸钙的溶解量为138.72 mg.L-1,分泌IAA(3-吲哚乙酸)及有机酸量分别为14.44 mg.L-1、46.00 mmol.L-1。菌株革兰氏染色为阴性,细胞短杆状;淀粉水解、吲哚、V-P(二乙酰试验)、苯丙氨酸脱氨酶、明胶液化及M-R(甲基红)试验呈阴性;柠檬酸盐、过氧化氢酶、硫化氢及硝酸盐还原等试验呈阳性,结合菌株16S rDNA序列分析结果,初步鉴定为肠杆菌(Enterobacter sp.)。该菌株在研制高效微生物磷肥接种剂方面可能具有较大潜力。