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1.
矮牵牛ECE 支TCP 基因的克隆及表达分析   总被引:1,自引:0,他引:1  
 ECE 支TCP 基因调控植物的分枝和花的对称性,但其功能在不同物种间存在差异。利用简 并PCR 法结合RACE 技术从矮牵牛(Petunia hybrida Vilm)GL8 自交系中获得了4 个矮牵牛ECE 支TCP 基因家族成员的全长cDNA 序列,分别命名为PhTCP1 ~ 4(登录号:JQ400104 ~ JQ400107)。cDNA 和 基因组序列分析表明,PhTCP1 ~ 4 分别编码406、332、341 和343 个氨基酸,PhTCP1 不含内含子,其 余3 个基因含1 ~ 2 个内含子。进化树分析发现,PhTCP1 ~ 4 分属于CYC1、CYC2 和CYC3 分支。荧光 定量PCR 分析表明,PhTCP1 ~ 4 均在成株期矮牵牛腋芽中优势表达,在不同节位腋芽中表现的表达趋势 各不相同,提示矮牵牛ECE 支TCP 基因可能主要与腋芽的生长发育相关  相似文献   
2.
育苗基质中适量的养分供应是培育优质壮苗的关键.选用东北草炭和蛭石4:1配制基质,运用正交试验设计,加入不同浓度的氮、磷、钾肥料,进行了万寿菊和矮牵牛穴盘育苗研究.结果表明,不同浓度配比氮、磷、钾肥料对两种草花种苗生长的影响不同.低氮中磷钾(O.1kg/m3 N、0.4kg/m3 P2O5、0.4kg/m3K2O)和低氮高磷钾(0.1kg/m3 N、0.8kg/m3 P205、0.8kg/m3 K2O)下,2种草花生长最快,接近或超过进口基质;万寿菊和矮牵牛种苗分别以低N中P高K(N 0.2kg/m3、P205 0.4 kg/m3、K20 0.8kg/m3)和中N高P高Km 0.4kg/m3、P205 0.8kg/m3、K20 0.8kg/m3)育苗效果最好.  相似文献   
3.
矮牵牛Tidal Wave品种遗传转化受体再生体系的建立   总被引:3,自引:0,他引:3  
以矮牵牛Tidal Wave品种无菌苗叶片为外植体,在附加不同质量浓度激素的MS基本培养基上诱导培养,通过器官发生途径获得再生植株。在附加3.0mg/LBA、0.3mg/LNAA的培养基上获得了90%以上芽的再生率。再生芽在附加0.02mg/LNAA、1.0mg/LKT、5.0mg/LGA3的培养基上发育良好。在3种选择抗生素的筛选中发现,叶片对潮霉素和庆大霉素较为敏感,这2种抗生素质量浓度为5mg/L时,2周内可将外植体全部杀死;而卡那霉素质量浓度达到15mg/L时,芽分化才被完全抑制。因此可确定卡那霉素为理想的选择抗生素,其选择压质量浓度为15mg/L。2种抑菌抗生素中,头孢霉素对叶片再生影响较大;而250mg/L的羧苄青霉素能有效地抑制农杆菌菌株LBA4404的生长,却对矮牵牛叶片的芽分化影响不大,为适宜的抑菌抗生素。  相似文献   
4.
选用东北草炭和蛭石4:1配制基质,运用正交试验设计,加入不同浓度的氮、磷、钾肥料,进行了万寿菊和矮牵牛穴盘育苗研究。结果表明,不同浓度配比氮、磷、钾肥料对两种草花种苗生长的影响不同。低氮中磷钾(0.1 Kg/m3 N、0.4 Kg/m3 P2O5、0.4 Kg/m3 K2O)和低氮高磷钾(0.1 Kg/m3 N、0.8 Kg/m3 P2O5、0.8Kg/m3 K2O)下,两种草花生长最快,接近或超过进口基质;万寿菊和矮牵牛种苗分别以低N中P高K(N 0.2 kg/m3、P2O5 0.4 kg/m3、K2O 0.8 kg/m3)和中N高P高K(N 0.4 kg/m3、P2O5 0.8 kg/m3、K2O 0.8 kg/m3)育苗效果最好。  相似文献   
5.
Dana  Michael N.  Ascher  Peter D. 《Euphytica》1985,34(2):237-244
Summary Discriminating styles (DS) in Petunia hybrida discriminate among incompatible pollen sources with pollen-mediated pseudo-self compatibility (PMPSC) similarly to those in Nemesia strumosa. Both species exhibit a single-locus gametophytic self-incompatibility system. Correlation of PMPSC to PSC level of the male was not universal and DS/PMPSC operated in heterozygous S genotypes. PSC levels of progenies generated from compatible or incompatible bud pollination were not significantly different from those of open-flower pollination of a DS plant with a given male, suggesting that DS did not select among male gametophytes for increased PSC.Scientific Journal Series Paper Number 13,077 of the Minnesota Agricultural Experiment Station.  相似文献   
6.
Flavonoids, in particular the anthocyanins,are responsible for flower colour in manyspecies. The dihydroflavonols represent abranch point in flavonoid biosynthesis,being the intermediates for production ofboth the coloured anthocyanins, through theaction of the enzyme dihydroflavonol4-reductase (DFR), and the colourlessflavonols, produced by flavonol synthase(FLS). In this study the white-flowered,flavonol accumulating Mitchell line ofpetunia was used as a model to examine theinteraction between DFR and FLS enzymeactivities and possibilities forredirecting flavonoid biosynthesis awayfrom production of flavonols and towardsanthocyanins. Introduction of a 35SCaMV-DFR sense transgene construct causedthe production of anthocyanins, resultingin a pink-flowered phenotype. Furthermore,inhibition of FLS production throughintroduction of an FLS antisense RNAconstruct also led to anthocyaninproduction and a pink-flowered phenotype. A combination of both transgenes gave thehighest level of anthocyanin formation. Anthocyanins were produced in the DFR-senseand FLS-antisense transgenic lines in spiteof the greatly reduced levels of geneexpression in the Mitchell line for threeenzymes late in anthocyanin biosynthesis,anthocyanindin synthase, UDP-glucose:flavonoid 3-O-glucosyltransferase andUDP-rhamnose: anthocyanidin-3-glucosiderhamnosyltransferase. Thus, the level ofgene activity required for visibleanthocyanin formation is much lower thanthe high levels normally induced duringpetal development. Altering the balancebetween the DFR and FLS enzyme activities,using genetic modification, may be a usefulstrategy for introducing or increasinganthocyanin production in target ornamentalspecies.  相似文献   
7.
Summary SI inbreds of P. hybrida and crosses between self-incompatible (SI) petunia plants were brought to flower under winter and summer glasshouse conditions. SI response, as measured by self seed, ranged from zero seed set under both conditions to low or zero seed set during the summer and high seed set during the winter. Some plants produced comparable seed yields during either pollination time. Increased expression of pseudo-self-compatibility (PSC) during the winter months was attributed to a breakdown of the Si system by the low light conditions of Minnesota winters (45°N). Genotypic differences within and between populations in the winter provided differences facilitating selection for increased SI. These differences were masked in some populations by the summer environmental conditions. The use of simulated or natural low light conditions as a technique for selecting for SI should increase selection efficiency.Scientific Journal Series Paper Number 10,499 of the Minnesota Agricultural Experiment Station.  相似文献   
8.
Summary Anthers of male fertile, cytosterile and restored male fertile clones of Petunia hybrida were compared for esterase activity and composition in subsequent stages of microsporogenesis. Three methods were applied (i) ultra-thin layer isoelectric focussing on polyacryl amide gels, (ii) quantitative spectrophotometrical assay, (iii) histochemical determination of total esterase activity associated to the azo-coupling method (Pearse, 1972).In male fertile and restorer idiotypes the isozyme patterns were quite similar. Both the number and intensity of bands increased gradually till the tetrad stage. In contrast, esterase activity in cytosterile anthers remained at a low level and hardy any new bands showed up in later stages. This unvariable, low activity level in cytosterile anthers was also found in the spectrophotometric assay. Histochemical determinations revealed that in male fertile anthers esterase activity is concentrated in the outer tapetal layer at late prophase and that it accumulates there till the early microspore stage. In male sterile anthers esterase accumulation in the tapetal cells stops at the moment that tapetal breakdown becomes visible. This suggests that differences in esterase activity and composition are an effect rather than a cause of the failing pollen formation.  相似文献   
9.
最近研究证明,在叶绿体中超表达甲基营养菌固定甲醛关键酶HPS-PHI的融合蛋白(由rmpAB基因编码)是提高转基因天竺葵代谢和吸收甲醛(HCHO)的一个有效策略。以rmpAB转化模式观赏植物矮牵牛,经基因组PCR分析确认5个转基因株系,RT-PCR检测结果表明rmpAB基因在这5个株系的叶片中可以正常转录。选取3个转录水平高的株系进行Western分析,结果证明融合蛋白HPS-PHI能在这3个转基因株系的叶片中表达,表达的融合蛋白HPS-PHI有生物学活性,酶活性最高的株系(P26)是野生型的2.5倍。经检测,3个转基因株系对液体甲醛的吸收速率均高于野生型。用P26株系进行H14 CHO标记试验,结果证实融合蛋白HPS-PHI的超表达也增强矮牵牛同化HCHO的能力。在含有7、10mmol.L-1甲醛的MS培养基上,P26的生长优势很明显,气体HCHO处理也观察到类似的结果,表明转基因矮牵牛HCHO抗性增强。证明应用该策略提高转基因观赏植物代谢和吸收HCHO的能力具有可行性。  相似文献   
10.
矮牵牛的快速繁殖技术   总被引:9,自引:0,他引:9  
以矮牵牛为试材,筛选了快速繁殖优良苗木时的最适基本培养基浓度及激素条件.结果表明分化培养时的最适培养基为1/4 MS+ BA0 .5 mg/L+IBA0 .02 mg/L;增殖培养时为MS+ BA0 .5mg/L+ IBA0 .2 mg/L;诱导根系培养时为1/2 MS+ IBA0 .5 mg/L+ 活性炭100 mg/L.生根培养基中添加活性炭有效地促进了矮牵牛根系的生长和发育,同时促进了叶片和茎的分化生长.  相似文献   
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