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Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila   总被引:1,自引:0,他引:1  
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A nested polymerase chain reaction (PCR) system was developed for the detection of Flexibacter maritimus from fish tissue. The total procedure for the diagnosis of marine flexibacteriosis, from the point of DNA extraction to the electrophoretic analysis, can be performed in < 4 h. This was achieved by the combination of a short thermal cycling programme with a rapid DNA extraction procedure. The assay was extremely sensitive, capable of detecting as few as 75 cfu mg(-1) fish tissue. The accuracy of the nested PCR was confirmed under field conditions using tissue samples recovered during 1993-2002 from fish suffering marine flexibacteriosis. The nested PCR method proved to be efficient for the rapid and sensitive detection of F. maritimus from fish tissues and can be used for routine diagnosis of the disease caused by this pathogen.  相似文献   
4.
Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL−1 of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g−1 tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   
5.
Dynamic Monitoring of the Mud   总被引:2,自引:0,他引:2  
[Objective] The research aimed to provide basic files and theoretical guidance for constructing sluicing-siltation dam using soil with high clay content soil.[Method] The soils of Dagou basin near Xiwu Village of Baishui County,Shaanxi Province were taken as experimental materials.pvc pipes with same height and diameter were used to construct testing model for dynamically determining settlement,shear strength,wet density of grouting bulk under 2 different grouting speeds(15 cm/d and 25 cm/d).[Result] Under different grouting speeds,general change trend was similar during grouting course.The subsidence,deformation,shear strength and wet density increased with the increase of grouting speed.Five or six days after grouting,daily displacement under 25 cm/d grouting speed was fewer than that under 15 cm/d grouting speed.[Conclusion] The increase of grouting speed could shorten the time for reaching the same subsidence,deformation,shear strength and wet density and increased displacement at the initial stage of grouting,however,with the increase of grouting time,lower grouting bulk was bad for displacement at later grouting period because it was near impermeable layer.  相似文献   
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Weed Detection Using Canopy Reflection   总被引:1,自引:0,他引:1  
For site-specific application of herbicides, automatic detection and evaluation of weeds is desirable. Since reflectance of crop, weeds and soil differs in the visual and near infrared wavelengths, there is potential for using reflection measurements at different wavelengths to distinguish between them. Reflectance spectra of crop and weed canopies were used to evaluate the possibilities of weed detection with reflection measurements in laboratory circumstances. Sugarbeet and maize and 7 weed species were included in the measurements. Classification into crop and weeds was possible in laboratory tests, using a limited number of wavelength band ratios. Crop and weed spectra could be separated with more than 97% correct classification. Field measurements of crop and weed reflection were conducted for testing spectral weed detection. Canopy reflection was measured with a line spectrograph in the wavelength range from 480 to 820 nm (visual to near infrared) with ambient light. The discriminant model uses a limited number of narrow wavelength bands. Over 90% of crop and weed spectra can be identified correctly, when the discriminant model is specific to the prevailing light conditions.  相似文献   
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GB19725—2005《便携式割灌机和割草机安全要求》中,附录C抛物试验是GB/T14176—2003《便携式割灌机和割草机技术条件》3.6安全要求中重要的检测项目。根据抛射物体的形状和抛射物体试验的要求,开发设计了抛射物体试验台,以完善割灌机安全性能试验项目的检测。  相似文献   
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Exploring and utilizing resistant germplasm resources plays a pivotal role in breeding for disease resistance, while screening resistant germplasm is important for selecting and breeding varieties resistant to disease. In the present study, we used PCR to detect the ratoon stunting disease (RSD) bacterium Leifsonia xyli subsp. xyli (Lxx) in 137 sugarcane core germplasms from the National Nursery of Sugarcane Germplasm Resources (NNSGR, Kaiyuan, China) in 2009, 2010 and 2011. A total of 21 germplasms that tested negative for Lxx in 2009 and 2010 were selected for further Lxx detection after being subjected to artificial inoculation in 2011 and 2012. The 21 core germplasms that were negative for Lxx after natural infection and artificial inoculation can provide elite resistance source materials and reference frames for the effective breeding of RSD-resistant sugarcane varieties. Under natural conditions, 116 (84.67%) and 21 (15.33%) out of 137 germplasms were positive and negative for Lxx, respectively, as determined by PCR detection, which suggests that a relatively high ratio of sugarcane core germplasms was sensitive to RSD, while few were resistant to RSD. The sequencing and analysis of 30 randomly selected PCR products showed that all 30 sequences were identical or highly homologous to the corresponding Lxx genome region published in GenBank (99.54–100% similarity). Lxx can be detected effectively and precisely by PCR. We therefore recommend PCR as a rapid, low cost and simple procedure to score sugarcane core germplasms for RSD resistance.  相似文献   
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This study aims to determine the authenticity of the geographical origin of rice using inductively coupled plasma atomic emission spectroscopy (ICP-AES) and chemometrics. The profiles of 25 elements in brown rice measured by ICP-AES were subjected to data-mining processes, including principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). PLS-DA clearly discriminated the geographical origin of rice samples grown in three countries. Eleven elements (Cu, Ag, Zn, Cr, Ca, Ba, Cd, Bi, K, Pb, and In) significantly contributed to the ability to discriminate the geographical origin of the rice. These results demonstrate the use of multielement profiling combined with chemometrics as a tool for discriminating food origins. This study extends our knowledge about the applications of both multielement profiling and chemometrics for the determination of food authenticity, and thus can be useful for controlling the geographical origin of rice by governmental administration and protecting consumers from improper domestic labeling.  相似文献   
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