黄瓜花叶病毒(CMV)2个山西分离物CP序列测定及亚组分类分析

在生物学检测的基础上,利用酶联免疫检测、非序列依赖性PCR(sequence-independent amp-lification,SIA)对感病番茄进行分子鉴定,表现卷叶和花叶症状的番茄均被黄瓜花叶病毒(Cucumber mosaic virus,CMV)侵染,分离物分别命名为SXFQ(GenBank登录号为JX993914)和FQ(GenBank登录号为JX993912)。为明确其分类地位,对二者外壳蛋白(coat protein,CP)进行克隆和测序分析。应用DNAMAN软件对本课题组前期检测的7个CMV山西分离物、SXFQ、FQ以及其他3个CMV典型分离物CP序列进行比较分析,发现核苷酸和氨基酸序列最大相似性分别为77.1%~100%和81.6%~100%。氨基酸序列系统进化分析表明,9个CMV山西分离物属于CMV亚组I B的2个分支,其中在指示植物上表现较强症状的SXFQ与其他5个分离物为一分支,在指示植物上表现较弱症状的FQ与其他2个分离物为另一分支。对9个山西分离物CP进行亚组分类分析,结果表明其理化性质、稳定性、疏水性与预测结果相近,2个分支的分离物分别出现相近的氨基酸变异和蛋白结构,存在一定规律性。     

黄瓜花叶病毒浙江白术分离物全基因组序列测定与分析

正白术(Atractylodes macrocephala Koidz.)为菊科(Compositae)苍术属(Atractylodes)多年生草本植物,其根茎入药具补脾健胃、燥湿利水、止汗安胎等功效,在我国广泛栽培,并以于术(浙江临安于潜)品质最佳[1]。近年来白术生产由于品种单一,长期连作等因素导致病毒病害日趋严重。据报道,黄瓜花叶病毒(Cucumber mosaic virus,CMV)~([2])、蚕豆萎蔫病毒2号(Broad bean wilt virus 2,     

分数次Hardy算子的交换子在Lipschitz空间上的端点估计

主要研究分数次Hardy算子和Lipschitz函数生成的交换子在Lipschitz空间上的端点估计.分数次积分算子的方法不适用于分数次Hardy算子,将给出新的方法,同时也将考虑分数次极大算子的交换子的结果.     

基于主成分分析的海南植胶区砖红壤亚类土壤质量评价

[目的]研究海南植胶区砖红壤亚类土壤质量及其变化,并对其土壤质量进行评价。[方法]采用主成分分析法,以少数的综合变量取代原有的多个变量。[结果]第1主成分中,主要以pH、有机质、全N和有效Mo的因子载荷量较大,均达0.600以上。第2主成分中,以代换性Ca、代换性Mg和有效Cu的因子负载量最大,均达0.600以上。第3主成分中,载荷量较大的因子为速效P。提取的3个主成分累计方差贡献率达79.649%,表征了79.648%的土壤质量信息;第1主成分为土壤常规分析因素,第2主成分为土壤阳离子交换性能因素,第3主成分为土壤速效P因素。[结论]土壤速效P为影响海南植胶区砖红壤亚类土壤质量的一个重要因素。     

福州市周边蛋鸡场禽白血病亚群的血清流行病学检测

应用ELISA方法对分别采集自福州市周边30个蛋鸡场的597羽海兰蛋鸡、512羽罗曼蛋鸡、486羽伊莎蛋鸡和553羽特佳蛋鸡共2148羽的血样进行禽白血病病毒(ALV)亚群ALV-A/B和ALV-J抗体检测.结果表明:样品ALV-A/B抗体阳性率由高至低依次为:特佳蛋鸡群11.39%(63/553)、罗曼蛋鸡群11.32%(58/512)、海兰蛋鸡群8.54%(51/597)和伊莎蛋鸡群7.61%(37/486);样品ALV-J抗体阳性率由高至低依次为:特佳蛋鸡群5.24%(29/553)、罗曼蛋鸡群4.10%(21/512)、伊莎蛋鸡群3.51%(13/486)和海兰蛋鸡群3.18%(19/597);而样品ALV-A/B抗体总阳性率为9.73%(209/2148),高于ALV-J抗体总阳性率3.82%(82/2148),其中有0.70%(15/2148)的ALV-A/B和ALV-J抗体双阳性样品;蛋鸡场ALV-A/B抗体阳性率为70.00%(21/30),高于ALV-J抗体阳性率36.7%(11/30),其中有16.7%(5/30)的ALVA/B和ALV-J抗体双阳性样品.结论:福州市周边蛋鸡场不同品系和不同鸡群间存在不同程度的ALV流行,相比ALV-J,ALV-A/B流行更普遍,且存在ALV-A/B和ALV-J抗体双阳性样品,应予重视.     

PI3K/Akt信号转导通路在ALV-J感染中作用的初步研究

 【目的】探讨ALV-J在宿主细胞中复制与PI3K/Akt信号转导通路的关系。【方法】将血管瘤病变型ALV-J毒株HN06和骨髓瘤病变型ALV-J毒株NX0101分别感染DF-1细胞，通过Western blot、Real-time PCR、IFA和ELISA等方法，观察细胞Akt蛋白磷酸化水平、病毒RNA表达水平和病毒蛋白表达水平等指标。【结果】HN06株和NX0101株在体外细胞中复制水平有差异。HN06株的早期感染可引起Akt转导通路的活化，病毒引起的Akt磷酸化具有病毒滴度依赖性，而且能被PI3K特异性抑制剂LY294002所抑制，表明HN06株诱导的Akt活化是PI3K途径依赖的。LY294002可在病毒感染早期呈剂量依赖性地显著降低受染细胞中HN06 RNA水平、囊膜蛋白水平和细胞培养物上清中的病毒粒子含量。【结论】PI3K/Akt信号转导通路活化对HN06株在细胞感染早期具有重要的作用，该结果与已报道的有关细胞PI3K/Akt信号转导通路参与NX0101株的早期感染的结论一致。本研究为进一步阐明ALV-J入侵宿主细胞和复制的精确机制等研究奠定了基础。     

Response of hot pepper (Capsicum annuum L.) to mulching practices under planted greenhouse condition

Mulch is considered a desirable management technology for conserving soil moisture, improving soil temperature and soil quality. This study aimed to investigate soil conditions and hot pepper (Capsicum annuum L.) performance in terms of leaf photosynthetic capacity, fruit yield and quality, and irrigation water use efficiency (IWUE) under such practices in greenhouse condition. A field experiment across 3 years was carried out with four types of mulch (without mulch [CK], wheat straw mulch [SM], plastic film mulch [FM], and combined mulch with plastic film and wheat straw [CM]). Mulch could improve soil physical properties regardless of mulch materials. FM and CM treatments improved soil moistures status and soil temperature in comparison to CK control, while SM increased soil water content and decreased soil temperature. Mulch increased leaf net photosynthesis rate (P_N), stomatal conductance to water vapor (gs), intercellular CO₂ concentration (Ci), and transpiration rate (E), but declined instant water use efficiency (WUEi). No significant effect of mulch application on chlorophyll fluorescence was existent for the entire growth season. Fruit yield and irrigation water use efficiency (IWUE) showed some increment under all the mulch conditions. Compared to CK, the yield was enhanced by 82.3%, 65.0%, and 111.5% in 2008; 38.1%, 17.4%, and 46.5% in 2009; and 14.3%, 6.5%, and 19.6% in 2010 under SM, FM, and CM conditions, respectively. Although FM produced better fruit quality than other treatments, CM is the recommended practice for hot pepper cultivation in greenhouse condition due to working well to facilitate soil condition (moisture and temperature), plant growth, and marketable yield.     

公路绿化主要树种滞尘潜力模拟试验研究

利用绿化植物滞尘已成为缓解大气颗粒污染的最有效途径,选择具有代表性的常见公路绿化树种,通过对其叶片滞尘潜力的探讨研究,以期筛选出滞尘能力强的植物,为公路交通环境的改善提供理论依据。结果表明,不同植物的滞尘潜力差异显著,阔叶乔木中二球悬铃木、杨树、国槐叶片的滞尘潜力最大,分别为6.495 9 g/m2,5.736 8 g/m2,4.791 9 g/m2;常绿乔木中,侧柏是较好的滞尘树种,最大滞尘量为7.568 8 g/m2;灌木植物以忍冬叶片的滞尘能力最强,其次为紫丁香及卫矛,最大滞尘量分别为7.084 7 g/m2,6.062 5 g/m2,5.878 9 g/m2.     

An improved rotor side control strategy of double fedwind turbine generation system

Based on a on site wind turbine’s output active power vs. generator speed curve, the shortage of conventional power control strategies of doubly fed wind turbine generation system are analyzed. Considering the generator speed restriction and active power fast track control requirement, the power decoupling control strategy in the rotor side converter and variable pitch control strategy are improved, that is, an output mechanical torque of wind turbine and the generator speed of DFIG are used in the rotor side active power control loop in order to achieve an accurate optimal speed control, as well as a direct active power control method is also adopted in the variable pitch control strategy. Finally, the overall operational performances of DFIG wind turbine generation system using the proposed control strategies are simulated, and also compared with the conventional control strategies. The results show that the improved control strategy is more valid and correct.