首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1328篇
  免费   202篇
  国内免费   102篇
林业   8篇
农学   21篇
基础科学   1篇
  11篇
综合类   403篇
农作物   24篇
水产渔业   275篇
畜牧兽医   815篇
园艺   67篇
植物保护   7篇
  2023年   19篇
  2022年   48篇
  2021年   63篇
  2020年   62篇
  2019年   78篇
  2018年   45篇
  2017年   92篇
  2016年   81篇
  2015年   56篇
  2014年   52篇
  2013年   102篇
  2012年   106篇
  2011年   91篇
  2010年   72篇
  2009年   56篇
  2008年   53篇
  2007年   58篇
  2006年   54篇
  2005年   54篇
  2004年   48篇
  2003年   38篇
  2002年   32篇
  2001年   32篇
  2000年   21篇
  1999年   18篇
  1998年   17篇
  1997年   23篇
  1996年   15篇
  1995年   21篇
  1994年   17篇
  1993年   14篇
  1992年   13篇
  1991年   14篇
  1990年   10篇
  1989年   16篇
  1988年   7篇
  1987年   5篇
  1986年   4篇
  1984年   2篇
  1983年   3篇
  1982年   1篇
  1981年   1篇
  1980年   4篇
  1979年   1篇
  1977年   1篇
  1976年   2篇
  1975年   1篇
  1974年   4篇
  1973年   1篇
  1956年   2篇
排序方式: 共有1632条查询结果,搜索用时 15 毫秒
1.
旨在评价不同剂量伊曲康唑-环糊精制剂通过皮下注射途径对健康比格犬肝肾功能及血常规的影响。选用体重±10 kg的健康纯种比格犬32只,按试验要求随机分为4组,每组8只,建立稳定的生活制度。分别按照0.25 mg/kg、0.75 mg/kg和1.25 mg/kg剂量,皮下注射伊曲康唑制剂,3日1次,连续7次,对照组注射生理盐水。于试验过程中第0、5、10、15、19天采集相关样品,对犬临床表现、血常规指标、血液生化指标、尿常规指标及肝肾功能进行全面的评价。结果表明,给药组与对照组相比,除白细胞相关指标外,其余指标都在正常范围内。给药组在试验过程中均出现白细胞升高现象,但在试验结束后5 d内均恢复正常。因此,伊曲康唑-环糊精制剂作为犬皮下注射剂型,具有良好的安全性和耐受性,操作方便,动物依从性好,适合兽医临床应用。  相似文献   
2.
[Objectives]This study aimed to explore the protective effect of Oxalis coriniculata L.on rats with acute liver injury induced by carbon tetrachloride(CCl4)and ...  相似文献   
3.
MA Wei  YANG Jia-yao  AN Liu  ZOU Qi  ZHANG Xiao  LIU Nian 《园艺学报》2021,36(12):2258-2263
AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease (NAFLD) rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups: control group, model group, high dose (20 mg·kg-1·d-1), middle dose (10 mg·kg-1·d-1), low dose (5 mg·kg-1·d-1) Fuzilizhong decoction group and Yishanfu (30 mg·kg-1·d-1)group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2(IL-2), IL-6 and tumor necrosis factor-α(TNF-α) were measured by ELISA. The expression of toll like receptor 4(TLR4) and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group(P<0.05). However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced(P<0.05).In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.  相似文献   
4.
AIM:To investigate the protective effect of ethanol extract from Cortex Albiziae on acute liver injury, and to explore its possible mechanism. METHODS:Acute liver injury in mice was induced by single intraperitoneal injection of 25% carbon tetrachloride (olive oil solubilization). The effective parts of ethanol extract from Cortex Albizziae against acute liver injury were screened. The pathological changes of the liver tissues were examined by pathological sections with HE staining. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) of the liver tissues were detected, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were mea-sured by ELISA, and the protein expression levels of NF-κB p65, Bcl-2 and Bax in the liver cells of the mice in each group were determined by Western blot. RESULTS:Compared with model group, the serum levels of AST and ALT in low-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-L, 4 mg·kg-1·d-1) group and high-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-H, 8 mg·kg-1·d-1) group were significantly decreased. The necrosis extent and degree of the hepatocytes and infiltration of inflammatory cells were significantly lower than that in model group. Compared with model group, the serum levels of TNF-α and IL-6 in AB-H group and AB-L group were significantly decreased (P<0.05). The protein level of NF-κB p65 in the nuclei of mouse liver cells in AB-H group and AB-L group were also decreased significantly (P<0.05). Compared with model group, the protein expression of Bax was decreased, the protein expression of Bcl-2 was increased, and the Bcl-2/Bax ratio was increased in AB-L group and AB-H group. CONCLUSION:The n-butanol phase of ethanol extract from Cortex Albiziae may protect the liver by reducing the activation of NF-κB p65, inhibiting the excessive release of inflammatory cytokines IL-6 and TNF-α, and decreasing hepatocyte apoptosis via regulating Bcl-2 and Bax expression.  相似文献   
5.
旨在研究宫内发育迟缓(IUGR)对断奶仔猪胰岛素水平和肝发育的影响,以及日粮添加80 mg·kg-1双氢青蒿素(DHA)对其修复作用。本试验选取10头正常出生重(NBW)仔猪和20头IUGR仔猪,分为NBW组、IUGR组和IUGR-DHA组,每组10头仔猪。NBW组和IUGR组饲喂基础日粮,IUGR-DHA组饲喂基础日粮+80 mg·kg-1 DHA。所有仔猪21日龄断奶后饲喂相应日粮至49日龄,试验期为29天。结果显示,与NBW组相比,IUGR组显著降低了血清空腹葡萄糖(FBG)和胰岛素生长因子1(IGF-1)含量以及肝重量(P<0.05),显著提高了空腹胰岛素(FINS)含量和胰岛素抵抗(HOMA-IR)指数以及肝脏指数(P<0.05)。同时,IUGR组断奶仔猪肝组织形态结构受损,出现明显的空泡化,细胞器结构受损,线粒体和内质网明显肿胀,细胞核严重变形。与IUGR组相比,IUGR-DHA组显著升高了肝重量和IGF-1含量(P<0.05),显著降低了血清FINS含量和HOMA-IR指数,且肝组织形态结构得到明显改善。与NBW组相比,IUGR断奶仔猪显著下调了肝中IGF1、胰岛素受体底物1(IRS1)、磷脂酰肌醇-3激酶(PI3K)和蛋白激酶B(AKT2)的mRNA相对表达量(P<0.05)。与IUGR组相比,IUGR-DHA组显著上调了断奶仔猪肝中IRS1和AKT2的mRNA相对表达量(P<0.05)。结果表明:DHA对IUGR导致的断奶仔猪胰岛素抵抗以及肝发育受损具有一定的修复作用。本研究为畜牧生产中对IUGR的早期治疗以及DHA的推广应用提供了一定的理论依据。  相似文献   
6.
本试验旨在研究骆驼乳清蛋白(CWP)对热应激(HS)大鼠肝损伤、氧化应激及肝功能的保护作用。选取6周龄SD大鼠36只,适应性饲养2周后随机分为6组:正常对照组饲喂基础日粮;CWP对照组添加400 mg·kg-1的CWP;HS组除饲喂基础日粮外,每天进行2 h HS处理,连续8 d;3个CWP干预组分别于每次HS前灌服100、200和400 mg·kg-1的CWP。试验结束后,取大鼠肝组织,HE染色观察肝组织病理学变化,同时检测肝功能生物标志物、氧化应激标志物水平及抗氧化酶活性。结果表明:CWP降低了HS大鼠血清谷草转氨酶(AST)和谷丙转氨酶(ALT)活性,400 mg·kg-1的CWP干预效果最好(P<0.01);400 mg·kg-1CWP干预组有效降低了HS诱导的大鼠肝组织病理学改变; CWP降低了大鼠肝活性氧(ROS)及丙二醛(MDA)水平,增加了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及谷胱甘肽(GSH)水平,400 mg·kg-1的CWP干预效果最好(P<0.01)。结果提示,CWP干预能够以剂量依赖性方式降低大鼠肝氧化应激,增加抗氧化系统防御能力,从而缓解HS所致大鼠肝损伤。  相似文献   
7.
试验旨在建立蛋鸡脂肪肝综合征病理模型。选取5日龄的海兰褐蛋鸡公雏280只,随机分为对照组(基础日粮组)和3个模型组(A、B、C组),试验第1~10天给模型组饲喂不同配比的高脂饲料,第11~20天饲喂常规基础日粮,每天观察并记录试验鸡精神状态、外观体征、饮水量和食欲情况,试验第0、10、20天从各组随机抽取15只鸡进行翅静脉采血并剖取肝脏和腹脂,测定肝脏系数、肝脂率、腹脂率,血清总胆固醇(TC)、甘油三酯(TG)、谷丙转氨酶(ALT)和谷草转氨酶(AST)水平。结果显示,试验第10天,3个模型组剖检时可见腹腔和肠系膜有大量的脂肪沉积,肝脏系数、肝脂率和腹脂率,以及血清中总胆固醇、甘油三酯、谷丙转氨酶和谷草转氨酶指标均符合鸡脂肪肝综合征模型的诊断标准;试验第20天,模型A、B组谷丙转氨酶和谷草转氨酶水平有所恢复,其他指标仍高于对照组,而模型C组的临床症状和各项检测指标仍符合鸡脂肪肝综合征模型的诊断标准。因此采用连续饲喂高脂饲料C(74.5%基础日粮、6%胆固醇、14%猪油、5%蔗糖、0.5%丙基硫氧嘧啶)可成功建立蛋鸡脂肪肝综合征模型。  相似文献   
8.
国家级名中医王行宽教授善于从心肝肾三脏论治糖尿病,认为心肝火旺、耗伤阴血为发病之本;心火亢盛,肝气郁结,郁火燔灼游行三焦,内外皆病,故善饥消渴;肾精亏损,阴阳不调,津液不布,燥热内生,发为消渴则是疾病演变的必然结果。王教授临床采用自拟消渴方清肝泻心、补益肝肾、滋阴润燥治疗消渴,获满意疗效。  相似文献   
9.
蒋益兰教授为湖南省名中医,从事中西医结合防治肿瘤的临床、科研工作30余年,认为乳腺癌的发生发展与肝郁、脾虚、肾亏、冲任失和密切相关,主要病理因素为痰、瘀、毒,从平调肝气、健脾化痰、补肾滋阴、调补冲任出发,以四君子汤、逍遥散、二至丸等方药加减,佐以白花蛇舌草、半枝莲、重楼、莪术、鸡血藤、土贝母、夏枯草、猫抓草、生牡蛎、山慈菇等治疗乳腺癌。  相似文献   
10.
A 3‐year‐old, spayed female, Domestic Shorthair cat presented with anorexia, lethargy, vomiting, probable hemoabdomen, and multiple masses on the right lateral liver lobe. Clinicopathologic and imaging abnormalities included anemia, azotemia, icterus, and hepatomegaly with hypoechoic masses. On cytologic evaluation of a fine‐needle aspiration of a liver mass there was abundant extracellular pink‐ to purple‐colored material between hepatocytes. The amorphous material was stained with direct fast scarlet (DFS), and green birefringent areas were observed under polarized light, confirming the presence of amyloid. A unique finding on the cytologic smear were macrophages containing amorphous and fibrillar amyloid‐like protein. Histopathologic examination using H&E and Congo red staining confirmed amyloid deposits within the space of Disse, along the sinusoids, portal tracts, blood vessel walls, and within the cytoplasm of macrophages. Immunohistochemical staining with anti‐AA amyloid antibodies further confirmed the presence of AA amyloid. To the author's knowledge, this is the first report of the cytologic finding of AA amyloid protein within macrophages and DFS stain detection of amyloid on a cytologic smear.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号