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1.
为建立黑水虻幼虫与收集、输送、分离等机械工作部件间发生碰撞时的碰撞模型,基于黑水虻幼虫生物特性,应用Hertz弹性碰撞理论推导了黑水虻幼虫碰撞过程动力学方程,结合运动学方程原理构建了黑水虻幼虫恢复系数的测定装置并进行了黑水虻幼虫恢复系数测定试验。试验针对第5龄期的黑水虻幼虫,采用L16(44×23)混合正交试验方案研究了碰撞材料、材料厚度、下落高度、碰撞角、跌落方向、含水率等因素对黑水虻幼虫恢复系数的影响,然后对碰撞材料、材料厚度、下落高度、碰撞角、跌落方向进行单因素试验,并获得了材料厚度、下落高度、碰撞角对恢复系数的影响规律与回归方程,且方程的决定系数均不小于0.9427。试验结果表明,影响黑水虻幼虫恢复系数的因素影响由大到小为:碰撞材料、下落高度、碰撞角、跌落方向、碰撞材料厚度、含水率,其中含水率对恢复系数影响不显著。单因素试验结果可得:黑水虻幼虫与Q235钢、铝合金、有机玻璃、橡胶等碰撞材料间的恢复系数依次降低,随下落高度的增大而逐渐减小,随材料厚度的增加而逐渐增大,恢复系数随碰撞角的增大而整体呈增大趋势,且横向跌落方向大于纵向跌落方向。该文研究结果可为黑水虻幼虫收集、输送、分离等机械相关工作部件优化设计提供参考依据。 相似文献
2.
不同杀虫剂及其组合对梨小食心虫初孵幼虫的毒力及田间防效 总被引:1,自引:0,他引:1
采用浸泡幼果法测定了6种杀虫剂及其两元复配组合对梨小食心虫初孵幼虫的毒力, 并将筛选出的增效组合在田间进行防效验证。结果表明, 阿维菌素、氯虫苯甲酰胺、高效氯氟氰菊酯、呋虫胺、噻虫嗪和溴氰虫酰胺对梨小食心虫初孵幼虫的致死中浓度(LC50)分别为:0.691、4.883、5.350、10.326、13.966和27.942 mg/L。两元复配组合中, 阿维菌素与呋虫胺复配组合(8∶1、2∶1、1∶1、1∶2和1∶4)、呋虫胺与氯虫苯甲酰胺复配组合(1∶4、1∶2、1∶1、2∶1和4∶1)、阿维菌素与氯虫苯甲酰胺复配组合(4∶1、1∶1和1∶4)和高效氯氟氰菊酯与呋虫胺复配组合(1∶4)具有增效作用。在梨小食心虫暴发为害年份、于梨果不同发育阶段进行田间防效验证, 阿维菌素与呋虫胺1∶4(12.5 mg/kg+50 mg/kg)、呋虫胺与氯虫苯甲酰胺2∶1(25 mg/kg+12.5 mg/kg)和阿维菌素与氯虫苯甲酰胺1∶1(12.5 mg/kg+12.5 mg/kg)对梨小食心虫的防效分别为75.02%~94.81%、50.04%~89.15%和42.90%~90.57%, 其中, 阿维菌素与呋虫胺(1∶4)的防治效果最好。 相似文献
3.
4.
桉树尺蠖是闽南地区桉树林中发生最严重的食叶害虫,为控制其危害,确定最佳防治适期,通过采用林间喷洒2%阿维菌素+苏云金杆菌粉剂的方法,比较第1代幼虫危害早期(5月2日)、危害中期(5月13日)和危害后期(5月30日)分别进行防治的效果,结果表明:在第1代尺蠖幼虫危害早期,每667 m2使用500 g药剂进行防治,5 d后虫口减退率为97.5%;危害中期每667 m2施用500 g药剂进行防治,5 d后虫口减退率为95.3%;危害后期每667 m2施用1000 g药剂进行防治,5 d后虫口减退率为83.9%,明显比早期和中期开展防治的低。危害早期和中期施药防治,第2代幼虫不产生危害;而危害后期开展防治,第2代幼虫仍有轻度危害。在危害中期以前防治对提高防治效果、减少损失有显著作用,同时还能减少农药的使用量。 相似文献
5.
基于30余年渤海鱼卵、仔稚鱼历史调查资料的整理分析并结合产卵场补充调查,以1982~1983年周年逐月调查资料为本底,采用多元统计学方法分析30余年渤海鱼类种群早期补充群体群聚特性(物种多样性和关键种群)的季节变化和年代际变化,并掌握结构更替过程中优势种和重要种协同消长规律。分析结果显示,渤海各调查季节(冬季除外)鱼卵、仔稚鱼种类数以及资源丰度指数呈先降后升变动趋势。当前鱼卵种类数仅为20世纪80年代1/2左右,资源丰度不足20世纪80年代的1/10;仔稚鱼种类数和资源丰度仅为20世纪80年代的3/4左右,但冬季仔稚鱼种类数和资源丰度指数呈现上升趋势。各调查时期相同季节鱼卵优势种变化不明显,但仔稚鱼优势种变化幅度超过鱼卵,底层重要经济种类早期补充群体优势度急剧下降;鱼卵和仔稚鱼物种多样性水平在升温季节较高而在降温季节较低,调查期内各季主要呈现先降后升变动趋势。鱼类早期补充群体种类更替现象明显,近年来种类更替率呈现明显加快趋势。各调查时期相同季节各适温类型产卵亲体种数均呈现先降后升变动趋势,但各适温类型种数所占比例和全年综合各适温类型种数所占比例基本稳定。各调查时期相同季节各主要栖所类型产卵亲体种类数也均呈现先降后升变动趋势,全年综合陆架浅水中上层鱼类种数所占比例升高,中底层和底层鱼类所占比例有所下降。近30年在多重外来干扰作用下,渤海鱼类早期补充过程各个关键环节已随其栖息地(产卵场)生境要素发生不可逆变化或变迁。渤海鱼类种群早期补充群体群聚特性和结构更替是环境-捕捞胁迫下鱼类群落内多重生态位的交替失调和渔业资源结构性衰退的具体表现。 相似文献
6.
Cryopreservation of trochophore larvae from the hard clam Mercenaria mercenaria: Evaluation of the cryoprotectant toxicity,cooling rate and thawing temperature 下载免费PDF全文
Aquaculture of hard clams Mercenaria mercenaria is a $65 million industry along the east coast and Gulf of Mexico coast in the United States. The goal of this study was to develop a preliminary protocol to cryopreserve trochophore larvae of hard clams. The objectives were to evaluate the: 1) toxicity of cryoprotectants, including dimethyl sulfoxide (DMSO), propylene glycol, ethylene glycol and glycerol, at 5, 10, 15 and 20% for exposure time of 1, 15, 30, 45, 60 and 75 min; 2) effects of cooling rates (5, 10, 20 and 30°C/min for the first trial; and 1, 3 and 5°C/min for second trial from 4 to ?80°C), thawing temperature (30, 40 and 50°C) and their interactions on post‐thaw viability. A basic protocol was concluded as: 15‐hr trochophore larvae mixed with DMSO or propylene glycol (5, 10%), equilibrated for 15 min, cooled in a programmable freezer from 4 to ?80°C at a cooling rate of 5°C/min and thawed at 50°C for 6 s. With this protocol, the immediate post‐thaw trochophore survival was 23 ± 14%, and survival to D‐stage was 27 ± 14%. This is the first report on larval cryopreservation in the hard clam and would have application for genetic breeding and seed production. 相似文献
7.
Immunostimulatory effects of dietary poly‐β‐hydroxybutyrate in European sea bass postlarvae 下载免费PDF全文
Andrea Franke Catriona Clemmesen Peter De Schryver Linsey Garcia‐Gonzalez Joanna J Miest Olivia Roth 《Aquaculture Research》2017,48(12):5707-5717
The stable production of high‐quality fry in marine aquaculture is still hampered by unpredictable mortality caused by infectious diseases during larval rearing. Consequently, the development of new biocontrol agents is crucial for a viable aquaculture industry. The bacterial energy storage compound poly‐β‐hydroxybutyrate (PHB) has been shown to exhibit beneficial properties on aquatic organisms such as enhanced survival, growth, disease resistance and a controlling effect on the gastrointestinal microbiota. However, the effect of PHB on the developing immune system of fish larvae has so far not been investigated. In this study, the effect of feeding PHB‐enriched Artemia nauplii on survival, growth and immune response in European sea bass (Dicentrarchus labrax) postlarvae was examined. Amorphous PHB was administered to 28‐day‐old sea bass postlarvae over a period of 10 days. The survival and growth performance were monitored, and the expression of 29 genes involved in immunity, growth, metabolism and stress‐response was measured. While the expression of the insulin‐like growth factor 1 (igf1), an indicator of relative growth, was upregulated in response to feeding PHB, the larval survival and growth performance remained unaffected. After 10 days of PHB treatment, the expression of the antimicrobial peptides dicentracin (dic) and hepcidin (hep) as well as mhc class IIa and mhc class IIb was elevated in the PHB fed postlarvae. This indicates that PHB is capable of stimulating the immune system of fish early life stages, which may be the cause of the increased resistance to diseases and robustness observed in previous studies. 相似文献
8.
The importance of dietary HUFA for meagre larvae (Argyrosomus regius; Asso, 1801) and its relation with antioxidant vitamins E and C 下载免费PDF全文
Najlae El Kertaoui Carmen María Hernández‐Cruz Daniel Montero María José Caballero Reda Saleh Juan Manuel Afonso Marisol Izquierdo 《Aquaculture Research》2017,48(2):419-433
Despite the interest of meagre (Argyrosomus regius) as a fast‐growing candidate for Mediterranean aquaculture diversification, there is a lack of information on nutrition along larval development. Importance of highly unsaturated fatty acids (HUFA) and the antioxidant vitamins E and vitamin C has not been investigated yet in this species. Six diets with two levels of HUFA (0.4% and 3% dw), two of vitamin E (1500 and 3000 mg kg?1) and two of vitamin C (1800 and 3600 mg kg?1) were fed to 15 dah meagre larvae. Larval growth in total length and dry body weight was significantly lowest in larvae fed diet 0.4/150/180 and showed few lipid droplets in enterocytes and hepatocytes and lower HUFA contents than the initial larvae. Increase in dietary HUFA up to 3%, significantly improved larval growth and lipid absorption and deposition. Besides, among fish fed 3% HUFA, increase in vitamin E and vitamin C significantly improved body weight, as well as total lipid, 22:6n‐3 and n‐3 fatty acids contents in the larvae. Thus, the results showed that 0.4% dietary HUFA is not enough to cover the essential fatty acid requirements of larval meagre and a high HUFA requirement in weaning diets is foreseen for this species. Besides, the results also pointed out the importance of dietary vitamin E and C to protect these essential fatty acids from oxidation, increase their contents in the larvae and promote growth, suggesting high vitamin E and C requirements in meagre larvae (higher than 1500 and 1800 mg kg?1 for vitamin E and vitamin C respectively). 相似文献
9.
Evaluation of dietary taurine concentrations in microparticulate diets provided to larval California yellowtail (Seriola dorsalis) postlarvae 下载免费PDF全文
Taurine is an important amino acid derivative for marine and freshwater fish, especially during early development. We investigated the range of taurine concentrations that influence the growth and survival rates of California yellowtail (CYT; Seriola dorsalis) during transition from live feeds to microparticulate diets, as well as the extent to which nutrient leaching from the microparticulate diets affects these ranges. We tested particle‐assisted rotationally agglomerated (PARA) particles with four levels of taurine: 4 (low taurine; LT), 45 (medium taurine; MT), 93 (high taurine; HT) and 122 g/kg (very high taurine; VHT). Our results showed that CYT postlarvae had no significant differences in growth, survival and feed consumption rates between the MT, HT and VHT treatments. However, it should be noted that the PARA particles containing 122 g/kg (VHT) taurine were especially prone to leaching and may have had taurine concentrations as low as 34.9 g/kg before they settled on the bottom of the tank. Therefore, the actual dietary taurine concentrations experienced by the larvae were likely lower than the initial dietary concentrations. More research is needed to evaluate the potential nutrient toxicity of elevated dietary taurine concentrations for marine fish larvae and juveniles. 相似文献
10.
Phenotypic effects of antibiotic‐induced axenity and egg disinfection in early larval European seabass (Dicentrarchus labrax L.) 下载免费PDF全文
Spyridon Nikolakakis Kristof Dierckens Peter Bossier Dominique Adriaens 《Aquaculture Research》2018,49(7):2536-2552
In the present study, the hypothesis that the difference in axenic conditions in the incubation and rearing environment of European seabass larvae induces size and shape effects on the specimens is tested. This difference is studied between xenic and axenic seabass larvae of DAH (day after hatching) 0, 5, 11 and 15. The axenic rearing protocol involves an egg disinfection with glutaraldehyde after the primary one with iodine in the hatchery, and the hypothesis that this secondary disinfection induces size and shape effects is also tested. In order to accomplish this, three egg and larvae treatments are included: “DA” (disinfected axenic), “DX” (disinfected xenic) and “NX” (nondisinfected xenic). Regarding the effect of antibiotic‐induced axenity, DA larvae exhibited larger bodies than both DX and NX on DAH 5 and 11. They also had a smaller yolk sac than DX at hatching, but consumed it slower. Towards the end of the experiment, DA larvae were thicker, but slightly more curved than DX and NX, which may be an abnormal shape, or a slightly more advanced ontogenetic stage. As far as egg disinfection, it had significant but very moderate shape effects on DAH 5 and 11, and disinfected larvae consumed their yolk sac faster. To the best of our knowledge, this study is one of the first to illustrate the subtle but significant size and shape effects caused by antibiotic‐induced axenity and secondary egg disinfection in the early larval stages, which suggest the existence of bacterial mechanisms that play a phenotypic role. 相似文献