不同饲养水平下蛋白质与氨基酸在阉牛小肠内消化率的研究

选择6头体重500～600kg ,安装有瘤胃、十二指肠前端和回肠末端瘘管的阉牛 ,分3期饲喂以玉米、棉籽饼和氨化稻草为基础原料配合的3种不同饲养水平的日粮 ,测定十二指肠、回肠和粪便中的粗蛋白质和各种必需氨基酸流量 ,计算粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和各种必需氨基酸在小肠内的表观消化率。结果显示 :随着饲养水平的下降 ,粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和亮氨酸、蛋氨酸、色氨酸、缬氨酸、胱氨酸的小肠表观消化率逐渐降低(P<0.05)。在每种饲养水平下 ,真蛋白的小肠表观消化率均大于粗蛋白质 (P<0.05) ;各种必需氨基酸的小肠表观消化率均有差异 ,精氨酸和亮氨酸的小肠消化率通常比真蛋白高 ,色氨酸和苏氨酸的小肠消化率比真蛋白低 ,但各种必需氨基酸的小肠表观消化率并没有一致的大小顺序。与日粮相比 ,十二指肠中的必需氨基酸组成发生了较大的变化 ;与回肠流量相比 ,粪便中的必需氨基酸排泄量也发生了较大的变化。     

高效液相色谱法测定齿毛芝中的碱基

采用250SS4.6-ODSZ-8/5色谱柱,体积分数20%的甲醇为流动相,紫外260nm检测,尿嘧啶,尿苷,腺嘌呤腺苷的保留时间分别为2.23min,2.97min,4.13min和6.74min,检测齿毛芝中碱基的摩尔比依次为1`.24:0.41:2.29:2.05.     

Essential Fatty Acids and the Cat

Abstract— The physiology of essential fatty acid metabolism in the cat is reviewed. Emphasis is placed on those aspects of the n:6 and n:3 fatty acids, their metabolites and interactions, which relate primarily to the skin. The functional roles, if known, of the fatty acids are discussed. Recent clinical research into the use of essential fatty acid supplements in the management of feline dermatoses is presented. Current indications for the therapeutic supplementation with essential fatty acids are summarised.     

高压电压互感器的可靠性数据统计分析

本文根据高压电压互感器现场运行的调查材料,用概率统计的方法求出其寿命函数,并估计其平均故障率,为高压电压互感器的可靠性管理提供依据和参考。     

ULTRASONOGRAPHIC FEATURES OF DIFFUSE HEPATOCELLULAR DISORDERS IN DAIRY CATTLE

Ultrasonography of the liver was performed in 200 Holstein-Friesian dairy cattle using a 3.5 MHz transducer with a linear array electronic scanner. Liver samples were taken, processed and examined microscopically and the fat occupying rate was calculated. The hepatic ultrasonograms were evaluated according to the presence of bright pattern, dark pattern, deep attenuation, vascular blurring and blurring of edges. Of the 200 animals, 96 had a normal liver, 63 had hydropic degeneration of the liver, 37 had fatty infiltration of the liver, 3 had liver dystrophy and I had hepatic amyloidosis, diagnosed through histopathological examination. Amyloidosis was characterized by bright pattern and blurring of edges. Liver dystrophy had higher percentages of bright pattern and blurring of edges than normal liver. Hydropic degeneration had higher percentages of dark pattern and blurring of edges than normal liver. Fatty infiltration had higher percentages of bright pattern, deep attenuation, vascular blurring and blurring of edges than normal liver. The present results suggest that different ultrasonographic patterns can be observed in various diffuse hepatocellular disorders in dairy cattle     

Serum bile acids: reference values in healthy dogs and comparison of two kit methods

Serum bile acid (SBA) reference intervals were established by use of a radioimmunoassay method for fasting dogs to be 0.2 to 4.3 micro mol/L (n = 60) and for 2 hour postprandial samples to be 0.6 to 24.2 micro mol/L (n = 37). The SBA reference intervals estimated using an enzymatic method were 0 to 8.6 micro mol/L for fasting (n = 26) and 0 to 29.8 micro mol/L for 2 hour postprandial samples (n = 36). The correlation between the two methods including samples from healthy dogs and clinical cases is good (n = 128, r = 0.82, p < 0.0001). The radioimmunoassay method is linear to 50 micro mol/L and the enzymatic method is linear to 100 micro mol/L, thus both methods require serum dilutions to be made in many cases of primary liver disease. The enzymatic method is less expensive and more convenient for use in a clinical laboratory but requires a greater sample volume (400 micro I) than the RIA method (50 micro I). Both methods have adequate precision and accuracy to be useful as diagnostic tests of liver function in dogs.     

Diurnal and individual variations in bile acids in the plasma of normal dairy cows

Blood samples were collected every 2 h during a 24 h period from 6 cows of one herd and 10 cows of another herd. In a third herd 9 cows were sampled every 2 h from 6 a.m. to 8 p.m. Concentrations of total bile acids, acetoacetate, glucose and free fatty acids were determined in blood plasma. A marked difference in individual bile acid concentrations and patterns of diurnal variation was found. For most cows the highest bile acid values were observed between 2 and 6 a.m. (overall mean (+/- SD) at 6 a.m.: 104 +/- 84 mumol/l, range: 20-307 mumol/l). Fourteen cows with a bile acid value greater than 90 mumol/l at 6 a.m. ("high BA") were characterized as a group by showing a pronounced decrease in the mean bile acid concentration after morning feeding. In the group of 11 cows with a 6 a.m. bile acid value less than 90 mumol/l ("low BA") the time of day did not contribute significantly to the bile acid variation. For the "high BA" group a nearly synchronous variation between the mean values of the 3 feeding dependent parameters (acetoacetate, glucose and free fatty acids) and the mean values of bile acids was found. The within animal coefficients of correlation between bile acids and the feeding dependent parameters were significantly higher in the "high BA" group than in the "low BA" group. No direct connection was found between bile acid levels and the quantity of concentrates fed or the individual milk yield.     

以豆粕为原料制备反刍动物饲用肽的研究

本文通过正交试验,选用碱性微生物蛋白酶,研究得出酶解法制备大豆肽的最佳工艺参数:豆粕预处理条件为90℃水浴加热10min,酶解条件为底物浓度5%(W/V)、加酶量5万单位/g蛋白质、温度50℃、pH值10、酶解时间5.5h。蛋白质水解率达到25%,平均肽链长度为4.0。制得大豆肽粗蛋白质含量66.83%(DM)。并对制得大豆肽和原料豆粕的氨基酸含量进行分析。     

山羊高床舍饲配套饲养技术探讨

山羊高床舍饲技术通过修建标准化山羊圈舍，实现山羊饲养过程中对山羊个体的有效控制和辅助，降低饲养劳动量和劳动强度；通过带动种草养羊、养羊与沼气结合，在实现山羊饲养的同时，促进了退耕还草、封山育林等生态建设项目的实施。     

Tall oil fatty acid inclusion in the diet improves performance and increases ileal density of lactobacilli in broiler chickens

1. Studies were conducted with tall oil fatty acids (TOFA) to determine their effect on broiler chicken performance and ileal microbiota. TOFA, a product originating from coniferous trees and recovered by fractional distillation of side-streams from pulp production, mainly comprises free long-chain fatty acids (~90%) and resin acids (~8%). Conjugated linolenic acids and pinolenic acid are characteristic fatty acid components of TOFA.

2. TOFA products at 750 mg/kg feed were tested in two 35-day broiler chicken trials, each using a wheat soya-based diet and with 12 replicate pens per treatment. In both trials, TOFA improved body weight gain at all time points (P < 0.001) and feed conversion efficiency during the first 21 days (P < 0.01). Two different dry TOFA formulations (silica carrier and palm oil coating) were tested and showed performance effects similar to liquid TOFA.

3. Ileal digesta of the broiler chickens was analysed for total eubacteria, Lactobacillus spp., Enterococcus spp., Escherichia coli and Clostridium perfringens on days 14 and 35. TOFA significantly increased total eubacteria and lactobacilli density on day 14 (P < 0.05). There was a significant positive correlation between these bacterial groups and broiler body weight on day 14 (P < 0.01).

4. A numerical reduction in C. perfringens was observed. In vitro growth inhibition studies showed that C. perfringens was strongly inhibited by 10 mg/l TOFA (P < 0.001), while common lactobacilli were resistant to >250 mg/l. The in vitro results were thus in line with in vivo observations.

5. The mechanisms behind the bacterial shifts and their role in performance improvement are unknown. Further purification of TOFA components is needed to identify the effective agents.