首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   2760篇
  免费   335篇
  国内免费   169篇
林业   152篇
农学   312篇
基础科学   43篇
  125篇
综合类   1121篇
农作物   371篇
水产渔业   21篇
畜牧兽医   178篇
园艺   157篇
植物保护   784篇
  2024年   6篇
  2023年   77篇
  2022年   139篇
  2021年   129篇
  2020年   146篇
  2019年   99篇
  2018年   83篇
  2017年   126篇
  2016年   129篇
  2015年   113篇
  2014年   117篇
  2013年   136篇
  2012年   182篇
  2011年   209篇
  2010年   153篇
  2009年   115篇
  2008年   121篇
  2007年   117篇
  2006年   102篇
  2005年   81篇
  2004年   82篇
  2003年   72篇
  2002年   77篇
  2001年   87篇
  2000年   65篇
  1999年   73篇
  1998年   44篇
  1997年   44篇
  1996年   38篇
  1995年   51篇
  1994年   39篇
  1993年   35篇
  1992年   47篇
  1991年   25篇
  1990年   28篇
  1989年   22篇
  1988年   14篇
  1987年   6篇
  1986年   4篇
  1985年   7篇
  1984年   2篇
  1982年   2篇
  1981年   2篇
  1980年   2篇
  1979年   4篇
  1978年   4篇
  1977年   2篇
  1962年   2篇
  1955年   4篇
排序方式: 共有3264条查询结果,搜索用时 15 毫秒
1.
为获得烟草镰刀菌根腐病拮抗真菌菌株,试验以河南省豫中烟区烟草根际土壤为试材,以主要致病菌尖孢镰刀菌Fusarium oxysporum为主要靶标,采用稀释涂布平板法分离获得土壤真菌371株,平板对峙培养法测定获得1株具有明显抑制效果的拮抗菌株,利用形态学和分子生物学的方法将其鉴定为棘孢木霉Trichoderma asperellum Tr-0111。抑制效果测定表明,该菌株对尖孢镰刀菌的抑制率最高可达93.13%,对根串珠霉菌、拟茎点霉、链格孢菌等其他8种烟草病原菌均具有较强的抑制效果。促生作用测定结果表明,菌株发酵液使烟草种子萌发率提高5.34%,烟苗根长增长率为62.39%,对盆栽烟草的最大叶面积和地上部鲜重也具有显著的促生效果。为进一步相关机理的研究和生防菌剂的研制奠定了基础。  相似文献   
2.
3.
水稻无人机撒肥系统设计与试验   总被引:3,自引:0,他引:3  
针对水稻穗肥撒施效率和机械化程度低的问题,为提高无人机撒肥均匀性,基于多旋翼无人机平台设计了一种水稻撒肥系统,确定了离心盘、流量调节装置等主要结构参数,同时应用EDEM软件仿真分析了离心盘转速、落入位置角、肥料流量、无人机飞行速度和飞行姿态对肥料分布的影响。试验表明:肥料分布呈同心圆时,撒肥有利于幅宽边界确定;幅宽随着离心盘转速的增加而增大,肥料分布峰值随流量增大由左侧逐渐变为右侧;撒肥均匀性随流量增加呈先增、后减的趋势,无人机俯仰角与横滚倾斜角对肥料分布均有影响,俯仰造成肥料向中部堆积,横滚使肥料向单侧堆积。肥料流量、无人机飞行速度和离心盘转速与落入位置角均存在交互作用,对撒肥均匀性有显著影响。当落入位置角为40°、离心盘转速为1 100 r/min、肥料流量为3 460颗/s、飞行速度为5 m/s时,撒肥性能最佳,此时变异系数为8.86%。田间试验结果表明,无人机施肥效率约为人工施肥的12.5倍。本研究为水稻穗肥的机械化施用提供了解决方案,可为水稻穗肥的无人机撒肥系统设计提供参考。  相似文献   
4.
镰孢菌是引起大豆根腐病的常见病原菌,传统检测病原镰孢菌的方法存在操作繁琐、时间长、成本高和 效率低等缺点,建立一种快速检测方法显得尤为重要。本研究基于翻译延伸因子序列(EF-1α)建立了检测镰孢菌 (Fusarium species)的多重PCR反应体系,检测了多重PCR体系灵敏度,模拟侵染样本验证多重PCR技术的可行性。 实验结果表明,该方法能够通过扩增片段的大小区分锐顶镰孢菌(Fusarium acuminatum)、尖孢镰刀菌(Fusarium oxysporum)、茄病镰孢菌(Fusarium solani)和禾谷镰孢菌(Fusarium graminearum)。灵敏度检测显示,最低检测基因 组DNA浓度达1×10-4 ng/μL;模拟侵染样本实验表明,使用镰孢菌和大豆基因组混合样本作为模板,在DNA浓度为 100 ng/μL时仍能准确检测出目的条带。因此。以翻译延伸因子序列为靶标建立的多重PCR技术,能够灵敏、特异 性地检测出该四种镰孢菌,可在复合侵染引起大豆镰孢菌根腐病的病原菌鉴定中准确检测。  相似文献   
5.
The goal of this research was to identify quantitative trait loci (QTLs) for potato tuber resistance to the soil- and seedborne bacterium Dickeya solani and for tuber starch content, to study the relationship between these traits. A resistant diploid hybrid of potato, DG 00-270, was crossed with a susceptible hybrid, DG 08-305, to generate the F1 mapping population. Tubers that were wound-inoculated with bacteria were evaluated for disease severity, expressed as the mean weight of rotted tubers, and disease incidence, measured as the proportion of rotten tubers. Diversity array technology (DArTseq) was used for genetic map construction and QTL analysis. The most prominent QTLs for disease severity and incidence were identified in overlapping regions on potato chromosome IV and explained 22.4% and 22.9% of the phenotypic variance, respectively. The second QTL for disease severity was mapped to chromosome II and explained 16.5% of the variance. QTLs for starch content were detected on chromosomes III, V, VI, VII, VIII, IX, XI, and XII in regions different from the QTLs for soft rot resistance. Two strong and reproducible QTLs for resistance to D. solani on potato chromosomes IV and II might be useful for further study of candidate genes and marker development in potato breeding programmes. The relationship between tuber resistance to bacteria and the starch content in potato tubers was not confirmed by QTL mapping, which makes the selection of genotypes highly resistant to soft rot with a desirable starch content feasible.  相似文献   
6.
马铃薯产业是宁夏“1+4”特色优势产业,是西吉县所有农作物中种植面积最大、涉及农户最多、比较效益最高的农民脱贫致富的主导产业。近年来西吉县马铃薯产业由商品薯种植大县向种薯繁育大县的转变,推动了农业增效,农民增收的快速发展。但各类土传性病害的发生影响了马铃薯产业的发展,同时磋商了农民种植的积极性。为了测定筛选出对马铃薯枯萎病和干腐病防治效果好、成本较低药剂,为大田防治提供科学依据。  相似文献   
7.
In Brazil, sour rot is an important postharvest disease on fruits and vegetables. Geotrichum candidum (synonym Galactomyces candidus) has been reported as the main species causing this disease. However, the identity of the causal agent is still uncertain. This research aimed to determine the identity of 165 fungal isolates associated with sour rot obtained from fruits and vegetables in Brazil, and to evaluate the effect of different temperatures on the incidence of sour rot on artificially inoculated tomato fruits. Based on the phylogenetic analyses of DNA sequences from the D1/D2 domain of the large subunit (LSU) rRNA gene and morphological analyses, 129 samples belonged to Galactomyces candidus, 15 to G. candidum var. citri-aurantii, 6 to G. phurueaense, 2 to Gal. pseudocandidus, 1 to Hyphopichia burtonii, 1 to H. khmerensis, 3 to Saccharomycopsis crataegensis, 1 to S. vini, 1 to Magnusiomyces tetrasperma, 1 to Trichosporon coremiiforme, and 1 to Zygoascus meyerae. Two new species were found, namely, Geotrichum solani (on potato) and Geotrichum spondiadis (on red mombin). All isolates were pathogenic when inoculated on healthy tomato fruits, including the new species of Geotrichum, which were also inoculated into their respective hosts, that is, potatoes and red mombins. To the best of our knowledge, this study is the first to report the presence of five other genera besides Geotrichum associated with sour rot on fruits and vegetables in Brazil, which demonstrates the diversity of fungi and yeasts associated with this disease.  相似文献   
8.
During 2017–2019, we observed Trichothecium spp. causing fruit rot in the field and in storage. This study was conducted to examine morphological differences of the species from different hosts, reveal the species’ potential host range, and evaluate the efficacy of five fungicides. Six strains of Trichothecium spp. isolated from nectarine, peach and walnut were selected. Although the colony morphology, mycelial growth rate and spore size differed among hosts, phylogenetic analysis based on the internal transcribed spacer and part of the 5ʹ end of the β-tubulin gene showed that all tested strains belonged to Trichothecium roseum. For its host range, 23 kinds of fruit were examined using T. roseum strain YT-1 as an inoculum; 10 kinds of fruit, including pear, apple, mango, Chinese chestnut, pepino melon, fig and durian, were susceptible to T. roseum, with minimum inoculation concentrations ranging from 104 to 105 spores/ml. The fungicides that most effectively inhibited the six isolates were fluazinam and fludioxonil, with EC50 values of 0.07–0.1 and 0.01–0.04 μg/ml, respectively, followed by difenoconazole (0.81–2.96 μg/ml), boscalid (5.43–13.51 μg/ml) and azoxystrobin (9.18–27.25 μg/ml). Improvement of the shelf life of nectarines held in plastic trays was explored using allyl isothiocyanate (AITC) against T. roseum YT-1. The application of 10 μl/L AITC significantly suppressed disease symptoms. The findings provide useful information for future disease emergency management in the field and for food preservation.  相似文献   
9.
The pea root rot complex is a major concern for green pea production worldwide. This study aimed at characterizing its composition and dynamics throughout a cropping season in northern France. To this end, fungi and oomycetes were isolated from green pea plant roots with symptoms sampled at the flowering stage in 22 fields in 2017, and at the pea emergence, elongation and flowering stages in two fields in 2018. Out of 646 isolates collected, 317 were identified using molecular markers. Fusarium oxysporum, F. solani and F. redolens were highly predominant. Pathogenicity tests separated the isolates into four aggressiveness groups. F. solani isolates were the most aggressive. Phylogenetic analysis of their TEF1 sequences showed that they mainly belonged to the F. pisi lineage, and that F. oxysporum isolates were genetically close to isolates from the UK that did not belong to the forma specialis pisi. In addition, several Clonostachys rhizophaga isolates are reported for the first time to cause pea root rot. The oomycetes were rarely found and were represented by a few Pythium spp. isolates. Lastly, this study shows that the fungal and oomycete communities associated with pea root rot change during the cropping season. The level of dissimilarity of the root-rot-associated communities decreased throughout the cropping season towards a more similar composition at the flowering stage, dominated by F. solani, F. oxysporum and F. redolens. The proportion of nonpathogenic to weakly pathogenic isolates decreased progressively during the growing season in favour of moderately to highly pathogenic isolates.  相似文献   
10.
罗远明 《猪业科学》2021,38(9):52-54
智慧养猪已成为生猪产业发展的新趋势,其核心主要聚焦于智能环控、智能饲喂和对猪群的视频监控等技术的应用。大数据采集是实现智能化的前提,因此,通过射频识别(RFID)技术建立猪只大数据是实现智慧养猪的基础。通过正确的猪只个体识别技术的选型,在不同生命周期给猪只选配合适的电子耳标,给猪只建立精准的身份证明。在不同阶段的数据采集场景中,通过识别电子耳标产生真实数据的关联,准确掌握每一头猪从出生到屠宰再到消费市场终端之间的信息,从而确保了动物健康养殖、疫病控制和食品安全,真正意义上实现智慧养猪。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号