生态文明教育系统:一个亟待完善的服务系统

生态文明教育是形成生态意识和生态行为的重要途径,是影响生态文明建设进程的重要因素。理想的生态文明教育系统是包含家庭教育、学校教育、企业教育和社会教育四大分系统在内的、拥有完整功能和流程的服务系统;各分系统在时间维度上相互衔接、在空间维度上相互独立,并在不同时空发挥各自的作用。但现行的生态文明教育系统尚未意识到其服务属性,在自我定位、系统设计和流程安排上仍按照传统教育模式进行,不利于形成节约资源和保护环境的生态意识和生态行为。因此,提出重新设计从受教者角度出发、以产生生态行为为根本目的的生态文明教育系统,是实现生态文明教育目标的有效途径。     

Metal leaching and toxicity of denitrifying woodchip bioreactor outflow—Potential reuse application

The denitrifying woodchip bioreactor is designed to remediate nitrate-rich water, including those produced from aquaculture effluents. Reuse of treated bioreactor outflows in recirculating aquaculture would offer considerable water savings and valuable alkalinity recuperation. However, such bioreactors may leach detrimental wood-bound contaminants, preventing outflow reuse. To determine water reuse potential, woodchip media from two hardwood species (white ash, Fraxinus americana; Norway maple, Acer platanoides) were evaluated for 206 d under a range of operating conditions (start-up, steady-state, reducing conditions, and drying-rewetting cycles) for a spectrum of potentially harmful dissolved contaminants. Aerated outflows also were evaluated for acute and chronic toxicity to the biologically sensitive invertebrate Ceriodaphnia dubia. Dissolved metal leaching subsided within the first few weeks of operation, though initial concentrations of copper and zinc were detected at concentrations of concern. Elevated concentrations of tannins-lignin and total ammonia nitrogen were detected throughout the study and were influenced by operational phase. Acute toxicity was not generally detected, though chronic toxicity was observed during drying-rewetting cycles in the maple outflows. The measured toxicity was not correlated with water chemistry, indicating an additive effect of several toxicants. Overall, significant differences in outflow water quality between ash and maple wood species were negligible. Results indicated that bioreactor outflows may be applicable for aquacultural reuse, though reusing outflows immediately following start-up or restarting after a dry period would not be recommended.     

Comparison of yield,fruit quality,antioxidant activity,and some phenolic compounds of white seedless grape obtained from organic,conventional, and integrated fertilization

The aim of this research was to determine the effect of three different fertilizing systems, including organic, conventional, and integrated ones, on the yield, fruit quality, antioxidant activity, and some phenolic compounds of white seedless grape. The study was carried out on several 6-year-old white seedless grapevine located in the Urmia Province (North-West Iran). The results showed that the fruit physical characteristics and yield were affected significantly by different fertilization systems. The highest yield was observed in the conventional fertilization, followed by organic and integrated systems. The leaf mineral contents were obviously dependent on the different fertilization used, whereas pH and titratable acidity were not affected. Based on our results, the highest values of antioxidant activity and total flavonoid were found in the organic and integrated fertilization systems, respectively. Also, the highest contents of total phenolic, catechin, and quercetin-3-galactoside were observed in the organic fertilization system. Overall, the highest nutritional quality and biochemical characteristics of white seedless grape were obtained in organic fertilization system, which improved antioxidant capacity by enhancing total phenolics, total flavonoids and valuable phenolic compounds.     

甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物纳米微球作为口蹄疫病毒核酸疫苗递送载体的特性评价

旨在对制备的甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物[poly （D，L-lactide-co-glycolide），PLGA]纳米微球作为口蹄疫病毒（foot-and-mouth disease virus，FMDV）核酸疫苗递送载体进行评价。采用西佛碱反应和元素分析制备具有一定取代度的甘露糖修饰的壳聚糖衍生物（mannose modified chitosan，MCS），然后，经双重乳化挥发法制备得到甘露糖修饰的壳聚糖PLGA纳米微球（MCS-PLGA-NPs）。采用纳米粒径仪检测MCS-PLGA-NPs粒径分布和表面电势（zeta）、扫描电镜考察其形态、琼脂糖凝胶电泳观察其对质粒的吸附和吸附质粒后抵抗核酸酶降解能力、CCK-8法检测MCS-PLGA-NPs的细胞毒性、激光共聚焦观察巨噬细胞对MCS-PLGA-NPs-质粒DNA复合物的摄取、荧光显微镜和Western blot验证MCS-PLGA-NPs加载质粒DNA在细胞中的表达。元素分析结果表明，成功制备了取代度为5%~10%的MCS。纳米粒径测定和扫描电镜结果表明，MCS-PLGA-NPs的zeta为正值、粒径分布均匀且形态规则呈球形。琼脂糖凝胶电泳结果显示，MCS-PLGA-NPs吸附质粒的能力随着其质量的增加而增强并且可以在一定程度上抵抗核酸酶降解质粒DNA。在细胞毒性试验中，不同浓度的MCS-PLGA-NPs与RAW264.7细胞共孵育24 h后，细胞存活率仍在85%以上。在细胞摄取试验中，用激光共聚焦显微镜可以明显观察到质粒DNA结合到纳米微球表面被RAW264.7细胞摄取。荧光显微镜和Western blot试验证明MCS-PLGA-NPs加载质粒DNA可以在细胞中进行表达。综上表明，本研究成功制备了MCS以及具有递送核酸疫苗能力的MCS-PLGA-NPs，为FMDV核酸疫苗的递送研究提供了新的方向和见解，也为该递送载体携带特定抗原靶向抗原递呈细胞表面甘露糖受体以及应用于动物免疫的研究奠定基础。     

Understanding root,tuber, and banana seed systems and coordination breakdown: a multi-stakeholder framework

Vegetatively propagated crop (VPC) seed tends to remain true to varietal type but is bulky, often carries disease, and is slow to produce. So VPC seed needs to be handled differently than that of other crops, e.g., it tends to be sourced locally, often must be fresh, and it is less often sold on the market. Hence, a framework was adapted to describe and support interventions in such seed systems. The framework was used with 13 case studies to understand VPC seed systems for roots, tubers, and bananas, including differing roles and sometimes conflicting goals of stakeholders, and to identify potential coordination breakdowns when actors fail to develop a shared understanding and vision. In this article, we review those case studies. The framework is a critical tool to (a) document VPC seed systems and build evidence; (b) diagnose and treat coordination breakdown and (c) guide decision-makers and donors on the design of more sustainable seed system interventions for VPCs. The framework can be used to analyze past interventions and will be useful for planning future VPC seed programs.     

大熊猫源枝孢样枝孢霉野生株(Z20)与突变株(Zt)部分生物学特性及药敏试验比较

为探明枝孢样枝孢霉野生株Z20与突变株Zt在生物学特性及药物敏感上的差异,将枝孢样枝孢霉Z20和Zt于PDA培养基上连续培养7 d,每天观察菌落形态及长势。将菌株Z20和Zt在沙氏液基上培养4 d后用透射电镜对其超微结构进行观察。采用硫酸-苯酚法测定枝孢样枝孢霉Z20和Zt的菌丝体多糖含量。运用96孔板微量稀释法测定5种常见抗真菌药物对枝孢样枝孢霉Z20和Zt的最小抑菌浓度。结果表明:在PDA培养基上菌株Zt的生长速率高于Z20,菌落颜色较浅,孢子数量减少。在透射电镜下,野生株Z20的菌丝壁厚度和菌丝宽度显著大于突变株Zt,且菌丝的含糖量也显著高于突变株Zt。药敏试验中,除灰黄霉素外,枝孢样枝孢霉Zt对5种药物的敏感性均高于Z20。     

An approach to determining forest areas with recreational potential: The case of forest areas around main transportation roads in the Maçka Region of Trabzon,Turkey

It is most important to determine areas with recreation potential that can permit sustainable use of forest resources and also meet recreational uses. Under the method described in this study, using the Geographic Information System (GIS), a spatial suitability model for recreational use was combined with a recreational priority perceptual suitability model that depends on principal component analysis (PCA) and is based on the perceptions of both recreational users and experts. Using principal component analysis, five priority components explaining 69.371% of the total variance were identified out of 12 perceptual suitability components. These components are scenic beauty, accessibility, the surface of the landscape, tree type, and forest stand condition. The recreation terrain suitability value of an area with recreational potential was assessed with the assistance of these five priority components. This enabled us to generate a recreation terrain suitability value equation and a recreation terrain suitability value scale for a potential recreation area. This applicability of this approach was demonstrated in the identification of potential recreation areas in Trabzon, Turkey.     

Fillet Quality and Processing Attributes of Postsmolt Atlantic Salmon,Salmo salar,Fed a Fishmeal‐free Diet and a Fishmeal‐based Diet in Recirculation Aquaculture Systems

Many studies have evaluated the adequacy of alternate ingredient diets for Atlantic salmon, Salmo salar, mainly with focus on fish performance and health; however, comprehensive analysis of fillet quality is lacking, particularly for salmon fed these diets in recirculation aquaculture systems (RAS). To this end, a study was conducted comparing fillet quality and processing attributes of postsmolt Atlantic salmon fed a fishmeal‐free diet (FMF) versus a standard fishmeal‐based diet, in replicate RAS. Mean weight of Atlantic salmon fed both diets was 1.72 kg following the 6‐mo trial and survival was >99%. Diet did not affect (P > 0.05) processing and fillet yields, whole‐body proximate composition(fat, moisture, protein), fillet proximate composition, cook yield, fillet texture, color, or omega‐3 fatty acid fillet content, including eicosapentaenoic acid and docosahexaenoic acid levels. Whole‐body ash content was greater in salmon fed the FMF diet. The FMF diet resulted in a wild fish‐in to farmed fish‐out ratio of 0:1 per Monterey Bay Aquarium's Seafood Watch criteria due to its fishmeal‐free status and use of lipids from fishery byproduct. Overall, fillet quality and processing attributes were generally unaffected when feeding a diet devoid of fishmeal to postsmolt Atlantic salmon cultured in RAS. [Correction added on 7 September 2017, after first online publication: the P value in Abstract has been changed from “P < 0.05” to “P > 0.05”.].     

The possibility of application of agglomerate elastomers (EPP) as media for biological bed in aquaculture

The aim of the study was to determine the possibility of experimental media (agglomerate elastomers EPP) application as biological media bed, which serves the purpose of water purification in recirculating aquaculture systems (RAS). RAS enables mass‐production of fish in small volume of water in a limited area. This involves the possibility of multiple usage of water during culture. However, for that purpose of maintaining proper physico‐chemical parameters, water purification from products of metabolism, especially toxic nitrogen compounds, is required. One of the simplest and most effective ways to achieve it is combining application of two types of water filtration: mechanical and biological. It is needed to study new media for biological bed with proper filling is able to purify water from toxic nitrogen compounds.     

长江中游地区水旱复种轮作模式资源利用率比较研究

为了实现农田资源高效利用,维护农业生态良性循环,优化长江中游地区传统种植模式,本研究于2013年10月—2015年11月,以冬闲连作为对照,分析比较了不同水旱复种轮作模式(冬闲-早稻-晚稻→冬闲-早稻-晚稻、马铃薯-玉米‖大豆-晚稻→蔬菜-花生‖玉米-晚稻、蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻、绿肥-早稻-晚稻→油菜-花生-晚稻、油菜-花生-晚稻→马铃薯-玉米‖大豆-晚稻)的光、温、水、土资源利用效率。结果表明:水旱复种轮作模式的冬季、晚季和周年的光能利用率均比冬闲连作处理高,周年光能利用率两年间分别高8.26%~82.50%和2.63%~121.42%,其中均以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式最高。水旱复种轮作模式的年有效积温利用率均高于冬闲连作模式,两年间分别高12.87%~21.26%和11.17%~25.88%,以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式最佳。两年间水旱复种轮作模式的冬季、晚季和周年的水分利用率均比冬闲连作处理高,其中周年水分利用率分别高45.36%~83.50%和40.00%~118.75%,以"马铃薯-玉米‖大豆-晚稻→蔬菜-花生‖玉米-晚稻"轮作模式的晚季和周年水分利用率最高,"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"模式的冬季水分利用率最高。两年间土地利用率均以"绿肥-早稻-晚稻"复种模式最高,平均利用率达96.11%,"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式的土地利用率表现最好。因此,各水旱复种轮作模式的周年光能利用率、年有效积温利用率、水分利用率和土地利用率均高于冬闲对照,其中以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"和"绿肥-早稻-晚稻→油菜-花生-晚稻"轮作模式表现较好,适宜在长江中游地区推广应用。