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The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine. 相似文献
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Cassiicolin毒素蛋白是橡胶树棒孢霉落叶病菌的主要致病因子。本研究对分离自海南省5个市县的橡胶树棒孢霉落叶病菌进行了鉴定,形态学鉴定结合分子生物学鉴定结果表明,病原菌为多主棒孢。利用Cassiicolin毒素不同亚型编码基因特异性引物和菌饼活体接种法分别鉴定了分离菌株的毒素蛋白类型和致病性,结果显示,分离的35个菌株都含有Cas5基因,未发现含其他类型的毒素基因,表明海南橡胶树棒孢霉落叶病菌为含Cas5毒素蛋白优势群体;所有菌株对橡胶树品种‘RRIM600’都能够致病,但是在不同抗性品种上致病性存在一定差异。 相似文献
5.
针叶树是芬兰、瑞典、挪威、美国、加拿大等欧美国家主要的商品林树种。容器苗是针叶树重要的育苗类型。与裸根苗比较,容器苗具有育苗周期更短、造林季节可延长、苗木适应性更广、苗木质量更一致的优点。因此,容器育苗成为精准林业发展的必然趋势。为了借鉴欧美容器育苗的技术方法,提高我国容器苗的质量,为我国针叶树容器苗发展提供参考,文中主要从4个方面综述了欧美国家检测与提高苗木质量的方法,即列举了针叶树常用的苗木质量检测指标,总结了育苗容器与育苗基质的改良进展,介绍了养分、水分、光照与温度等培养条件的优化手段,简要概述了容器苗病虫害防控的进展,提出了我国相关树种容器苗生产的启示与借鉴。 相似文献
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利用高脂饲料诱导建立巴马小型猪代谢疾病模型并分析肝脏转录组变化。选取1月龄巴马小型猪24头,随机分为对照组与高脂诱导组,对照组饲喂普通饲料,高脂诱导组饲喂含2.0%胆固醇饲料,定时监测体重、血常规及生化指标,6个月后试验结束,作心脏冠状动脉造影、器官组织学检测及肝脏转录组分析。结果表明,高脂诱导组个体饲喂4个月体重显著高于对照组,诱导期后血液生化指标中甘油三酯显著高于对照组,饲喂6个月时冠状动脉造影及器官组织切片未见显著病变,肝脏转录组分析显示高脂诱导组与对照组相比有2 468个差异表达基因,主要与代谢途径相关。为期6个月的高脂饲喂未致巴马小型猪明显代谢性疾病及心血管病变,但血液甘油三酯水平显著升高,显著影响肝脏代谢途径相关基因表达。文章系统探讨高脂诱导模型,结合血液生化、组织学、冠脉造影及肝脏转录组分析,进一步获得代谢通路相关潜在标记基因,为建立理想小型猪代谢疾病模型提供参考。 相似文献
7.
Vunjia TIONG Shih-Keng LOONG Haryanti Azura MOHAMAD WALI Kim-Kee TAN Pui-Fong JEE Fang-Shiang LIM Jing-Jing KHOO Pooi-Fong WONG Li-Yen CHANG Sazaly ABUBAKAR 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):280
Corneal lesions appearing as white mass beneath intact epithelium, with ocular discharge in one mouse, was observed in a batch of laboratory-raised BALB/c mice (n=9 of 56). The affected mice remained active, well-groomed and had normal appetite. Isolates recovered from swab cultures of the external and internal contents of the eye had partial 16S rRNA gene sequence of 99.1% similarity to Streptococcus cuniculi. No previous report of S. cuniculi infection in laboratory rodents has been presented. The isolate was susceptible to all antibiotics tested. We suggest S. cuniculi is an opportunistic bacteria in laboratory mice but are uncertain of its source. Our findings revealed that S. cuniculi is able to colonize laboratory mice and should be considered when mice present with eye lesion or ocular discharge. 相似文献
8.
M. A. Macedo A. K. Inoue-Nagata T. N. Z. Silva D. M. S. Freitas J. A. M. Rezende J. C. Barbosa M. Michereff-Filho A. R. Nascimento A. L. Lourenção A. Bergamin Filho 《Plant pathology》2019,68(1):72-84
Efficient management of whitefly-borne diseases remains a challenge due to the lack of a comprehensive understanding of their epidemiology, particularly of the diseases tomato golden mosaic and tomato yellowing. Here, by monitoring 16 plots in four commercial fields, the temporal and spatial distribution of these two diseases were studied in tomato fields in Brazil. In the experimental plots these diseases were caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV), respectively. The incidence of each virus was similar in the plots within a field but varied greatly among fields. Plants with symptoms for both diseases were randomly distributed in three of four spatial analyses. The curves representing the progress of both diseases were similar and contained small fluctuations, indicating that the spread of both viruses was similar under field conditions. In transmission experiments of ToSRV and ToCV by Bemisia tabaci MEAM1 (former biotype B), these viruses had a similar transmission rate in single or mixed infections. It was then shown that primary and secondary spread of ToCV were not efficiently controlled by insecticide applications. Finally, in a typical monomolecular model of disease progress, simulation of the primary dissemination of ToSRV and ToCV showed that infected plants were predominantly randomly distributed. It is concluded that, although the manner of vector transmission differs between ToSRV (persistent) and ToCV (semipersistent), the main dispersal mechanisms are most probably similar for these two diseases: primary spread is the predominant mechanism, and epidemics of these diseases have been caused by several influxes of viruliferous whiteflies. 相似文献
9.
大蒜根腐病根际土壤真菌群落结构及多样性分析 总被引:1,自引:0,他引:1
研究旨在解析根腐病发生时大蒜根际土壤真菌群落结构,明确大蒜根腐病与根际土壤真菌群落多样性变化的关系,探明根腐病发生的微生态机制,为病害的防控提供理论基础。以新疆连续3年的大蒜根腐病发病田的根际土壤为研究对象,采用高通量测序方法对大蒜根际土壤总DNA的真菌ITS区序列进行大规模测序分析。结果表明,与健康对照相比,在门的水平上,子囊菌门和接合菌门真菌是大蒜根腐病根际土壤的优势真菌类群,其中高水平的子囊菌门菌群与病害发生有着密切的关系。在属的水平上,注释获得137个属,其中19个属与大蒜根腐病有较大的相关性。镰刀菌属真菌数量在病土中高于健康对照。随着连作年限的延续,根腐病的发生愈发严重,土壤中真菌群落的Shannon指数、Simpson指数、真菌属的数量逐年降低,大蒜根腐病的发生与植株根际真菌群落组成及多样性密切相关,土壤真菌类群的平衡和多样性改变是根腐病发生的一大诱因。 相似文献
10.
Partho Pratim Debnath Jerome Delamare-Deboutteville Mona Dverdal Jansen Kornsunee Phiwsaiya Afsana Dalia Md. Abir Hasan Saengchan Senapin Chadag Vishnumurthy Mohan Ha Thanh Dong Channarong Rodkhum 《Journal of fish diseases》2020,43(11):1381-1389
Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV might be widespread, we executed a surveillance programme on tilapia grow-out farms and hatcheries from 10 districts of Bangladesh in 2017 and 2019. Among farms experiencing unusual mortality, eight out of 11 farms tested positive for TiLV in 2017, and two out of seven tested positive in 2019. Investigation of asymptomatic broodstock collected from 16 tilapia hatcheries revealed that six hatcheries tested positive for TiLV. Representative samples subjected to histopathology confirmed pathognomonic lesions of syncytial hepatitis. We recovered three complete genomes of TiLV from infected fish, one from 2017 and two from 2019. Phylogenetic analyses based on both the concatenated coding sequences of 10 segments and only segment 1 consistently revealed that Bangladeshi TiLV isolates formed a unique cluster within Thai clade, suggesting a close genetic relation. In summary, this study revealed the circulation of TiLV in 10 farms and six hatcheries located in eight districts of Bangladesh. We recommend continuing TiLV-targeted surveillance efforts to identify contaminated sources to minimize the countrywide spread and severity of TiLV infection. 相似文献