Investigation of environmental and land use impacts in forested permafrost headwaters of the Selenga-Baikal river system,Mongolia - Effects on discharge,water quality and macroinvertebrate diversity

Headwater streams play a major role for provision of ecosystem services, e.g. drinking water. We investigated a high-altitude headwater catchment of the Kharaa River (including 41 1st-order rivers) to understand the impact of land cover (especially forest cover), environment and human usage on runoff, chemical water quality and macroinvertebrate fauna in a river basin under discontinuous permafrost conditions in an arid, sparsely populated region of Mongolia. To verify our hypotheses that different landuses and environmental impacts in permafrost headwaters influence water quality, we investigated 105 sampling sites, 37 of them at intermittent stream sections without water flow. Discharge was positively impacted by land cover types steppe, grassland and forest and negatively by shrubland, forest burnt by wild fires (indicating a reduction of permafrost) and slope. Water quality was affected by altitude, longitude and latitude, shrub growth and water temperature. Shannon diversity of macroinvertebrates was driven by water temperature, iron content of the water, flow velocity, and subbasin size (adjusted R² = 0.54). Sample plots clustered in three groups that differed in water chemistry, macroinvertebrate diversity, species composition and bio-indicators. Our study confirms that steppes and grasslands have a higher contribution to runoff than forests, forest cover has a positive impact on water quality, and diversity of macroinvertebrates is higher in sites with less nutrients and pollutants. The excellent ecological status of the upper reaches of the Kharaa is severely threatened by forest fires and human-induced climate change and urgently needs to be conserved.     

Integrated Evaluation and the Physiological and Biochemical Responses of Semi-Wild Cotton under Complex Salt-Alkali Stress

[Objective] The genetic diversity of semi-wild cotton was abundant, so more new elite resistant genes could be found and applied to improve the resistance of cotton. The aim of this study was to primarily study on the regulation of response to salt-alkali stress, explore the method of integrated evaluation and characterize the resistance of semi-wild cotton. [Method] Four semi-wild and two cultivated Gossypium hirsutum accessions were evaluated for phonotypical, physiological and biochemical traits under alkali-salt versus normal conditions in hydroponic solutions. The alkali-salt tolerance was determined based on principal component analysis, cluster analysis, correlation analysis, grey rational analysis and analysis of variance. [Result] On the basis of the overall results, the alkali-salt stress tolerance of the six accessions was ranked as: Marie-galante85 > Latifolium32 > CRI16 > CRI12 > Latifolium40 > Latifolium130. Roots were found to be the most important responsive systems to complex alkali-salt stress. At seedling growth stage, the active scavenging system played a crucial role in response to alkali-salt stress. In addition, the salt tolerant and salt sensitive accessions showed different response tends towards leaf peroxidase, root ascorbate peroxidase and root catalase within 48 h, suggesting the accessions have different levels of response to salt stress. [Conclusion]Our study identified the most alkali-salt tolerant accessions and provided basic concept of complex alkali-salt tolerance mechanism within cotton accessions. And, our study provided a simple and rapid, highly accurate and precise method for evaluating salt resistance of cotton, and proved that the balance of ROS system play an important role in response to salt-alkali stress. Hence, mining of salt tolerant genes from these accessions may facilitate the development of novel salt tolerant variety.     

Using near‐infrared reflectance spectroscopy to predict the digestible protein and digestible energy values of diets when fed to barramundi,Lates calcarifer

This study examined the potential of using near‐infrared spectroscopy (NIRS) to predict nutrient digestibility parameters (digestible protein and digestible energy) of compound diets when fed to barramundi. A series of 60 diets were assessed for their protein and energy digestibilities in a series of five experiments over a 5‐year period from 2009 to 2014. Considerable variance was observed in the digestibility parameters of diets across the experiments, providing a suitable range in diet digestible protein and digestible energy values from which to develop a NIRS calibration. Samples of the same diets were also scanned using a diode array near‐infrared spectrophotometer (DA‐NIRS). The spectra were obtained by the DA‐NIRS and were chemometrically calibrated against the digestible value data using multivariate analysis software. The results in terms of standard error of cross‐validation (SECV), residual prediction deviation (RPD) and correlation coefficient (R²) show good relationships (R² > 0.8) between the predicted and observed parameters for both the digestible protein and digestible energy parameters assessed. This study therefore demonstrates that it is possible to use NIRS technology to provide rapid estimates of the digestible protein and digestible energy values of compound diets for barramundi in near real time.     

鲍蒸煮液复合调味品制作配方和工艺研究

为了提高鮑资源的利用率,开发新型香精,以鲍蒸煮液美拉德反应产物为基料,感官评定为指标,在单因素及L_9(3~4)正交试验基础上,确立了口感和海鲜风味俱佳的鲍复合调味品最佳配方为:盐添加量35%、味精添加量40%、玉米淀粉添加量9%、鲍风味基料添加量为6%、白砂糖添加量4%、麦芽糊精添加量3%、呈味核苷酸二钠(I+G)添加量1.5%、酵母抽提物添加量1.5%。研究了不同的造粒方式和干燥方法对颗粒产品吸湿性、溶解性、流动性、质构特性及感官评定的影响;相关理化、性能指标为:完全溶解于90~100℃热水中的时间需要72 s,且分散良好、溶液微浑浊、无沉淀,鲜味足;堆密度为(0.61±0.01)g/cm~3,硬度为(3 642±122.198)g,吸湿性为(9.35±0.01)%,临界湿度值为60%,休止角为33.0°,总体可接受度为(7.81±0.12)分。研究结果表明,旋转造粒和流化床干燥的产品质量最佳,吸湿性比较小,溶解性、流动性和质地性都比较好,感官评分最高。     

WSSV envelope protein VP51B links structural protein complexes and may mediate virus infection

White spot syndrome virus (WSSV), an enveloped double‐stranded DNA virus, is the causative agent of a disease that has led to severe mortalities of cultured shrimps in Taiwan and many other countries. In the previous study, Penaeus monodon chitin‐binding protein (CBP) and glucose transporter 1 (Glut1), two cell membrane proteins, were found to at least interact with other 10 WSSV envelope proteins including VP51B. These envelope proteins might form a protein complex. According to the known information, VP51B was used to identify its role in the protein complex. Western blotting of the intact viral particles and fractionation of the viral components confirmed that VP51B is one of WSSV envelope proteins. In this study, the protein–protein interaction between VP51B and other WSSV envelope proteins was identified by far‐western blot experiment and VP51B was found to interact with VP24, VP31, VP32, VP39B and VP41A. Furthermore, the in vivo neutralization experiment using recombinant VP51B plus with VP39B showed the best inhibition. These data indicate that VP51B participates in the WSSV protein complex and plays an important role in WSSV infection.     

广西田园综合体的建设现状和发展建议

文章通过对田园综合体的概念进行介绍，找出广西田园综合体发展情况和存在的问题，最后 提出解决广西田园综合体存在问题的对策和建议，从而促进广西农业的快速发展，带动当地经济稳步 提升。     

响应面试验优化霍山石斛原球茎增殖培养基

为可持续开发霍山石斛资源，采用响应面法优化霍山石斛原球茎增殖培养基。在单因素试验基础上，根据Box Behnken试验设计原理，采用三因素三水平的响应面分析法，以增殖率为响应值进行回归分析。结果表明，萘乙酸（简称NAA）及6 苄氨基嘌呤（简称6 BA）浓度对增殖率影响最为显著，6 BA与马铃薯两者间交互作用显著，适宜的NAA、6 BA及马铃薯浓度分别为0.05 mg·L-1、0.70 mg·L-1和185 g·L-1，此时增殖率为2 989.82%±205.55%，与模型预测值基本符合。响应面法优化霍山石斛原球茎增殖培养基切实可行。     

Effect of dietary chitosan oligosaccharide complex with Ce (IV) on growth,immunity and disease resistance against Vibrio splendidus of sea cucumber,Apostichopus japonicas

An 8‐week feeding trial was conducted to investigate the effects of dietary chitosan oligosaccharide complex with cerium (Ce IV) (COS‐Ce) on growth performance, nonspecific immunity and disease resistance of sea cucumber, Apostichopus japonicas. Five isonitrogenous (18.6%) and isolipidic (1.1%) practical diets were formulated with graded level of COS‐Ce (0, 150, 300, 600 and 1200 mg kg^?1 dry feed), which were named as COS‐Ce/0, COS‐Ce/150, COS‐Ce/300, COS‐Ce/600, COS‐Ce/1200 respectively. Each diet was allocated to four replicates of sea cucumbers (Initial weight: 6.72 ± 0.02 g). Sea cucumbers were fed to apparent satiation once daily (19:00 hours) for 56 days. During the experiment, water temperature was kept at 16 ± 0.5°C, pH 7.8–8.2, dissolved oxygen beyond 5 mg L^?1, ammonia nitrogen below 0.5 mg L^?1 and salinity from 30‰ to 31‰. Results showed that the specific growth rate of sea cucumbers was significantly higher in COS‐Ce/600 than that in other four treatments. Activities of phagocytosis, respiratory burst, acid phosphatase and alkaline phosphatase in COS‐Ce/600 were significantly higher than that in COS‐Ce/0 (P < 0.05) respectively. On the contrary, cumulative mortality was the lowest in COS‐Ce/600 following 14 days exposure to Vibrio splendidus (P < 0.05). In conclusion, these results confirmed that dietary COS‐Ce had beneficial effects on growth performance, nonspecific immunity and disease resistance of sea cucumber.     

猴头菇双层奶工艺和配方的研究

以猴头菇、牛奶、鸡蛋为主要原料研发一款猴头菇双层奶，通过感官评价的方法优化猴头菇双层奶的配方和工艺。结果表明，双层奶上层配方为：牛奶200 g、绵白糖38 g、菠萝汁62.5 g、全蛋液41 g、奶粉5 g、明胶1.2 g；下层配方是：牛奶200 g、菇浆24 g、奶粉2.88 g、绵白糖20 g、蛋清35 g、白醋0.2%；上层与下层质量比为8∶92。加工条件为：下层奶在90 ℃的蒸汽下加热35 min，加入上层奶，蒸20 min。按此工艺和配方制得的双层奶层次清晰，颜色分明，上层暖黄色、有弹性及菠萝芳香，下层纯白色、绵软顺滑、味道清淡。