Protective Role of Comfrey Leave Extracts on UV-induced Zebrafish Fin Damage

In zebrafish, UV exposure leads to fin malformation phenotypes including fin reduction or absence. The present study evaluated UV-protective activities of comfrey leaves extracts in a zebrafish model by recording fin morphological changes. Chemopreventive effects of comfrey leave extracts were evaluated using Kaplan-Meier analysis and Cox proportional hazards regression. The results showed that (1) the mean times of return to normal fin in the UV+comfrey (50 and 100 ppm) groups were 3.43 and 2.86 days and were quicker compared with that in the UV only group (4.21 days); (2) zebrafish fins in the UV+comfrey (50 and 100 ppm) groups were 2.05 and 3.25 times more likely to return to normal than those in the UV only group; and (3) comfrey leave extracts had UV-absorbance abilities and significantly reduced ROS production in UV-exposed zebrafish embryos, which may attenuate UV-mediated apoptosis. In conclusion, comfrey leaves extracts may have the potential to be developed as UV-protective agents to protect zebrafish embryos from UV-induced damage.     

牧草叶绿素含量与蛋白质含量的相关性研究

为探讨牧草中叶绿素含量和蛋白质含量的相关性,分别测定了苜蓿、聚合草、串叶松香草3种牧草初花期的叶绿素含量和蛋白质含量,并采用Duncan’s新复极差法进行多重比较分析。结果表明:不同牧草品种间的蛋白质含量和叶绿素含量存在差异,牧草叶绿素a、b、a+b含量及a/b间存在一定的相关性,苜蓿、聚合草、串叶松香草中蛋白质含量与叶绿素a含量、叶绿素a/b显著相关,从综合效应看,其相关系数分别为0.624、0.677。表明,影响牧草蛋白质合成的最主要因素是叶绿素a/b,其次是叶绿素a含量,叶绿素b含量影响较小。     

不同生育期串叶松香草和聚合草氨基酸瘤胃降解特性研究

[目的]研究不同生育期串叶松香草和聚合草氨基酸的瘤胃降解特性,为牧草适宜收获期的确定及其资源利用提供依据.[方法]以6头装有永久瘤胃瘘管的小尾寒羊为试验动物,用尼龙袋法测定不同生育期串叶松香草和聚合草在绵羊瘤胃降解前后氨基酸含量的变化,研究氨基酸的瘤胃降解特性.[结果](1)随生育期的推进,串叶松香草和聚合草中氨基酸含...     

添加聚合草对猪粪中温厌氧发酵的影响

【目的】探索添加景观植物聚合草对沼气厌氧发酵的影响。【方法】以猪粪为发酵原料,添加不同量聚合草,于(35±1)℃条件下,用自行设计的发酵装置进行发酵试验,通过对发酵液的CH4含量、pH和产气量的监测,研究聚合草添加量对猪粪中温厌氧发酵过程的影响,并以接种物和接种物加猪粪为参比。【结果】在聚合草与猪粪总固体含量比分别为1∶10、2∶10、3∶10、4∶10的试验组中,1∶10、2∶10试验组的甲烷含量率先达到40%(体积分数),其pH分别为6.6～7.7和6.6～7.6,总体优于其他试验组及空白参比组;且以1∶10试验组的日均产气量最高(756.20mL),2∶10试验组的总产气量最高(43 540mL)。【结论】添加适量聚合草对猪粪厌氧发酵有促进作用,通过二次曲线拟合,推测聚合草与猪粪总固体含量的最优配比为1.40∶10～1.48∶10。