Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue

Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR)in vitro, and the availability of purified toxin has facilitated development of immunological detection methods. A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to detect COR in various host plants infected by P. syringae. The estimated detection limit for COR was 50 pg per well, and COR could be reliably quantified from 5 to 40 ng ml⁻¹. The subcellular localization of COR within infected tomato tissue was investigated using the COR-specific antibody MAb 8H3G2. Immunofluorescence microscopy and immunogold labelling showed that COR was present inside tomato cells and was associated with chloroplasts and particles of proteinase inhibitor I. Localization studies indicated that COR is mobile in infected plant tissue and can be detected in healthy tissue adjacent to the bacterial lesions.     

Collard landraces are novel sources of glucoraphanin and other aliphatic glucosinolates

The glucosinolate make‐up of the edible parts of some Brassica oleracea L. crops has been investigated previously, but the leafy‐green collard (B. oleracea var. viridis) remains relatively unstudied on this topic. Due to this lack of information, a collection of US landraces was examined for glucosinolate content of leaves. The specific objectives of this examination were to compare levels of certain glucosinolates among the conserved collard landraces, identify any individuals with a distinct glucosinolate profile and determine the potential of collard as a target for chemoprotective‐based plant breeding. During winter 2010/2011, 81 collard landraces, four other viridis and four collard cultivars were evaluated in the field and harvested leaves were assayed for glucosinolates. In a subsequent study, 19 selected landraces plus the cultivars were included in a repeat trial in 2012/2013. Eighteen collard landraces contained relatively high levels of glucoraphanin in leaves in both years, and three (designated G 32575, G 32580, G 32586 in the US National Plant Germplasm System) were found to contain glucoraphanin in excess of 9.5 μmol/g DM. The examined landraces are rich sources of important aliphatic glucosinolates, previously thought to be most abundant in other B. oleracea vegetables.     

抱子甘蓝和羽衣甘蓝的核型分析

以抱子甘蓝和羽衣甘蓝为材料,采用常规压片法进行核型分析,结果表明:抱子甘蓝的核型公式为2n=2x=18=10+8sm(2SAT),其中第3、4、5、6、8对为中着丝粒染色体,第1、2、7、9对为近中着丝粒染色体,第7对染色体具有随体;相对长度组成为2n=2L+6M2+10M1;核型分类为2A型,属于基本对称型。羽衣甘蓝的核型公式为,2n=2x=18=10m+8sm,其中第3、4、6、7、8对为中着丝粒染色体,第1、2、5、9对为近中着丝粒染色体,未观察到随体染色体;染色体相对长度组成为2n=8M2+10M1;核型分类为2A型,属于基本对称型。     

观赏羽衣甘蓝新品种梦幻的选育

观赏羽衣甘蓝新品种梦幻是以青花菜细胞质雄性不育系CMS305为母本,观赏羽衣甘蓝自交系438为父本配制的杂交一代品种,该品种生长势好、抗病性强、耐寒性突出,观赏性状优良,显色早、抽薹晚,最佳观赏期长达130 d。