无核葡萄胚发育及早期离体培养的研究:Ⅲ.培养方式对离体胚发育的影响

对5个无核葡萄品种的试验表明,不同培养方式对离体胚发育的影响是很明显的,其中4个品种胚发育率液体培养高于固体培养,‘京早晶’在液体培养中胚发育率高达73.7%,是目前国内外胚发育率最高的报道;液体培养比固体培养胚的发育程度高,发育整齐,无畸形胚,易成苗.     

High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.     

精梳棉卷成卷过程中起泡问题分析

介绍了成卷工艺对棉卷结构的影响以及棉卷成卷过程中起泡现象与准备工艺的关系.分析了棉卷成卷工艺过程中起泡过程和起泡原因,提出了防止起泡的措施.     

影响番茄离体培养再生的主要因素探讨

以5个番茄品种的子叶和下胚轴为外植体,对无菌苗的生理状态、激素组合、激素浓度和基因型等影响组培效果的主要因素进行探讨,建立了番茄的高效离体培养再生体系。结果表明,下胚轴和子叶均得到了高达100%的出愈率和95%以上的分化率;最佳的品种是T431;最佳激素组合为ZT1.0mg·L-1和IAA1.0mg·L-1。     

Effects of Three Drugs on Membrane Metabolism Against Cysticerci cellulosae in vitro

To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one.     

八种中草药体外抑制大肠杆菌的效果观察

选取马尾松、田基黄、艾草、雷公根、芭蕉芯、浮萍、野菊花、番石榴叶8种中草药，采用常规中草药药敏试验打孔法和试管法对猪大肠杆菌（K88）、大肠杆菌（0157）进行体外抑菌试验。结果显示，艾草、野菊花、番石榴叶对大肠杆菌呈高度敏感，其中艾草对大肠杆菌抑菌力最强；马尾松挥发油对大肠杆菌呈中度敏感；田基黄、雷公根、芭蕉芯、浮萍对大肠杆菌无抑菌作用。     

大豆室内耐冷筛选及其在田间的应用研究

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。     

冷却水循环用离心泵汽蚀的原因与防止方法探讨

通过对冷却水循环用离心泵产生汽蚀的因素进行分析，提出了防止汽蚀产生的技术措施，经生产应用效果良好。     

矸酆恐?,垩白率与糙米率、蛋白

Χ灾绷吹矸酆康挠跋焓钦?,直接影响比间接影响大。Study on the correlation between rice quality and agronomic traits of quality riceNIE Cheng rong, WEN Yu hui, WANG Yun bo, CHEN Ming zhong,LI Mei, LIU Dao quan(Agronomy Department, Foshan University , Nanhai 528231,China)The correlative analy     

水闸管理的微机通信网络

在水闸管理上,以多台Tp801单板微型计算机、输入输出接口电路、SIO串行通信接口电路、Tp801D板、12吋绿色显示器、打印机等组成一种远距离传送数据的微机通信网络,它利用电话线路作为数据传输的通道,在淮阴市盐东控制工程管理处投付使用。运行稳定,性能可靠,于1985年9月通过技术鉴定。