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1.
The aim of this study was to evaluate the efficacy of Cervidil®, a prostaglandin E2 (PgE2)-releasing vaginal insert used for induction of cervical ripening and labour in women, to enhance the ease of transcervical artificial insemination (TCAI) in anestrous ewes (June). It was hypothesized that the use of Cervidil® prior to AI would cause dilation of the cervix, and thus alleviate the difficulty associated with traversing the cervix for semen deposition in sheep. Cervidil® was inserted 12 h before insemination in six Rideau-Arcott ewes; six ewes served as untreated controls.Semen was deposited into the uterus of all six treated ewes but TCAI was possible only in four of the six control ewes. It can be concluded that Cervidil® facilitated transcervical semen deposition in anestrous ewes. The treatment with Cervidil® has the promise of a technique to improve transcervical AI and to enable non-invasive embryo transfer procedures in sheep.  相似文献   
2.
目的探讨欣普贝生联合地西泮静脉推注用于足月妊娠引产的临床效果及母婴安全性。方法采用随机对照前瞻性研究方法,对正常单胎头位初产妇足月妊娠。宫颈评分小于6分,无瘢痕子宫,具有引产指征而无使用前列腺素禁忌症的孕妇100例,随机分成观察组50例和对照组50例,观察组阴道放人欣普贝生,有不规律宫缩时静推地西泮10mg,对照组阴道放人欣普贝生,用药12h观察用药前后宫颈Bishop评分变化及用药至分娩时问、阴道分娩率、剖宫产率、总产程、产后24h出血量和新生儿体重、Apgar评分及出现子宫过度刺激等情况的差异。结果观察组用药促宫颈成熟有效率(98%)明显高于对照组(72%)(P〈0.05);观察组总产程平均为468min,阴道分娩成功率占82%,对照组总产程平均648min,阴道分娩成功率66%,两组差异有显著性(P〈0.05);产后出血量、新生儿Apgar评分、新生儿体重两组差异无显著性。结论欣普贝生联合应用地西泮对足月妊娠引产安全有效。  相似文献   
3.
This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (κ = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route.  相似文献   
4.
Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results. The ideal methodology (frozen‐thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen‐thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen‐thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application.  相似文献   
5.
This case report describes an uncommon case of a congenitally incompetent cervix and a resulting pneumouterus in a 5‐year‐old maiden Quarter Horse mare. The mare was presented for artificial insemination at the Clinic for Animal Reproduction, Freie Universität Berlin, Germany. Routine vaginal and rectal examinations revealed a shortened cervix of 3 cm length and a dilated uterus filled with a considerable amount of air. During ultrasonographic examination, ovaries of normal size with regular cyclic activity were detected. Chromosomal analysis could not confirm any genetic anomalies such as an expected XO karyotype. The mare was excluded from breeding due to its anatomical constitution.  相似文献   
6.
目的初步研究宫颈外观与宫颈病变的关系.方法选取2005-01—2009-12月河北北方学院附属第一医院妇科所有宫颈病变患者临床资料,回顾性分析其临床资料,按病变程度分组,比较各组病变之间宫颈外观的构成比,并作分析.结果在所有阳性病例中宫颈光滑和轻度糜烂与中重度糜烂组比较,差异无统计学意义.但在高度病变和癌变中中重度糜烂...  相似文献   
7.
8.
目的 :了解不同临床分期、组织学分级子宫颈鳞癌组织中端粒酶活性。方法 :采用以 PCR为基础的端粒酶重复扩增方法及非变性 PAGE银染定性法检测不同临床分期、组织学分级子宫颈鳞癌组织中端粒酶活性。结果 :在子宫颈癌组织中端粒酶活性的表达率较正常宫颈、不典型增生宫颈组织的端粒酶活性高 (P<0 .0 1 ) ,且不同临床分期的子宫颈癌宫颈组织间端粒酶的表达率差异有显著性 (P〈0 .0 5) ,但不同病理分级的子宫颈癌宫颈组织间端粒酶活性的表达率差异无显著性 (P>0 .0 5)。结论 :端粒酶在子宫颈癌组织中呈现高表达 ,且与临床分期有关 ,端粒酶可望成为检测子宫颈癌的一个重要指标。  相似文献   
9.
目的探讨子宫颈冷刀锥切术和LEEP锥切术在子宫颈上皮内瘤变诊治中的临床意义。方法比较患者子宫颈冷刀锥切与宫颈LEEP锥切术后结果与术前多点病理活检结果符合率并比较治疗效果。结果子宫颈冷刀锥切病理与术前多点病理活检结果完全符合者有70例(81.4%);有差异者16例(18.6%),其中7例术前多点活检为CINⅡ和CINⅢ,而锥切为CINⅢ和微小早期浸润癌。子宫颈LEEP锥切术病理与术前多点病理活检结果完全符合者有45例(75%);有差异者15例(25%),其中5例术前多点活检为CINⅡ和CINⅢ,而锥切为微小早期浸润癌。65例为CINⅢ或以上,常伴有累及宫颈管腺体;同时累及2个象限例57例,伴有HPV感染132例;切缘病变残留率分别是CINⅡ(3.2%)和CINⅢ(8.1%)。结论在子宫颈上皮内瘤变的诊治中,子宫颈冷刀锥切在完整切除病变、低残留方面占有优势,而LEEP锥切术省时、简单、安全、价廉。  相似文献   
10.
目的:观察宫颈癌年轻患者卵巢移位术后卵巢内分泌的变化。方法:对10例年轻、早期宫颈癌患者在行根治术的同时行卵巢移位,其中7例移位到髂脊上2cm皮下或腹外斜肌筋膜下方,3例移位到结肠旁沟中上侧腹部。10例中8例术后1月行外照射放射治疗,总剂量40 ̄50Gy。10例术前及术后(包括放疗后)6个月、1a、2a用放免法行血清性激素包括雌二醇(E2)、孕酮(P)、睾酮(T)、促卵泡素(FSH)和促黄体素(L  相似文献   
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