紫花苜蓿组织离体培养的形态建成及其调控

本文对紫花苜蓿 (Medicago Sativa L .)组织离体培养并附加植物生长调节物质进行调控。研究表明 :紫花苜蓿愈伤组织的形成可分为 3个阶段 :启动期、分裂期及形成期。同时阐述紫花苜蓿愈伤组织的分化及其调控过程以及植物器官的发生 (苗的发生和根的发生 )。     

Genotypic differences in organogenesis from callus of ten triticale lines

Summary Callus was obtained from immature excised embryos of triticale using MS medium supplemented with 3 mg/l 2,4-D and 1 mg/l kinetin. The presence of 2,4-D was essential for continued callus proliferation. Plantlets were induced from the calli by sub-culturing on medium either devoid of auxin or containing 0.1 mg/l 2,4-D. The capacity to produce callus and to form organs and plantlets differed markedly among the genotypes used. Lines also had distinct response to presence and absence of 2,4-D in the regeneration media. The callus of most triticale lines used differentiated into organs more readily on MS medium supplemented with 0.1 mg/l 2,4-D than on medium without growth regulators. Very high frequencies (up to 75%) of plantlet regeneration were observed in several of the triticale lines studied.     

Genetic control of somatic embryogenesis in cotton petiole callus cultures

Summary Three commercial varieties (Acala SJ-5, Coker 312 and Paymaster 303) and three exotic accessions (T1, T25 and T169) of cotton (Gossypium hirsutum L.) were tested for ability to undergo somatic embryogenesis. Sections of split petiole were cultured on 3 media and evaluated for embryogenesis after 180 days. Embryogenic T25 and Coker 312 plants were selected and crossed in a diallel with non-embryogenic Acala SJ-5, Paymaster 303, T1 and T169 plants. F₁, F₂ and BC₁ populations were generated and tested for embryogenesis on a medium of MS salts and vitamins (1962) plus (per liter) 4.0 mg NAA, 1.0 mg Kn, 30 g glucose, 100 mg myo-inositol, 2.0 g Gelrite and 0.75 g MgCl₂. Segregation for both occurrence and magnitude of embryogenesis was observed, suggesting the action of more than one gene.     

Effect of the Substrates upon the Regeneration of the Engrafted Cuttings and the Yield of Engrafted Rooted Vines

Engrafted cuttings of the variety Rkaciteli on stocks B?×?R Cober 5BB and R?×?R 101-14, having endured open and covered stratification on different substrates, were planted in a polyethylene glasshouse on four artificial substrates after the postplanting preparation. The complex effect of the stock variety was examined, as well as the effects of the stratification methods and the substrates on the yield of engrafted vines. The characteristics of the regeneration of the engrafted cuttings during the stratification were established. The optimal substrates for stratification of the transplants and the growing of engrafted vines in glasshouses were defined.     

Factors influencing mansonone induction in elm callus by culture filtrate elicitors of Qphiostoma ulmi

Mansonone F (phytoalexin) production in elm callus induced by elicitors of Ophiostoma ulmi is affLited by cultural and experimental conditions. These factors include different fun gal isolates, fungal culture age, elicitor concentration, the time of elicitor treatment, the callus cu Ytured with or without light and the callus subculturing frequency.     

紫叶桃（Prunus persica f．atropurpurea）的愈伤组织诱导和培养

紫叶桃是重要的园林彩叶树种,叶色美丽,观赏期长,具有很高的园林应用价值。以紫叶桃的叶片和嫩茎段为外植体,用不同浓度生长调节物质进行愈伤组织诱导试验,建立紫叶桃的再生体系。结果表明,诱导愈伤组织时,MS＋2mg／LBA＋1mg／L2,4-D对叶片诱导效果最佳,其诱导率达80％;MS＋1mg／LBA＋1mg／L2,4-D对茎段诱导效果最佳,其诱导率达100％。在MS＋1mg／LBA＋1mg／L NAA培养基上愈伤组织能直接分化出不定芽。     

苎麻组织培养及遗传转化研究进展

概述了国内外苎麻组织培养的快速繁殖、器官发生、体细胞胚胎发生、原生质体培养、花药培养等几个方面以及遗传转化的研究进展,并对存在的问题和前景进行了讨论。     

不同浓度CPPU对欧亚旋覆花愈伤组织诱导的影响

以欧亚旋覆花(Inula britannica L.)无菌苗为材料,研究了不同浓度调吡脲(CPPU)对其叶片、根愈伤组织诱导的影响。结果显示,CPPU的浓度为0.10 mg/L时,叶片的愈伤组织诱导率为82.9%,且诱导出的愈伤组织生长好。CPPU的浓度为0.50 mg/L时,根的愈伤组织诱导率最高,达100.0%,愈伤组织生长最好,呈浅黄绿色,部分带有红色斑点,质地疏松,具光泽。